链霉菌HU2014诱导小麦抗性基因的表达

doi:10.6048/j.issn.1001-4330.2025.06.005

新疆农业科学 ›› 2025, Vol. 62 ›› Issue (6): 1337-1343.DOI: 10.6048/j.issn.1001-4330.2025.06.005

• 作物遗传育种·耕作栽培·生理生化 • 上一篇下一篇

链霉菌HU2014诱导小麦抗性基因的表达

朱红霞¹(), 胡林峰²(), 王琦琦¹, 张军高¹, 张少民¹, 周小云¹, 雷斌², 张志东³()

1.新疆维吾尔自治区农业科学院农业资源与环境研究所/新疆作物化学调控工程技术研究中心,乌鲁木齐 830091
2.新疆农业科学院农药试制中心/新疆作物化学调控工程技术研究中心,乌鲁木齐 830091
3.新疆维吾尔自治区农业科学院微生物研究所,乌鲁木齐 830091

收稿日期:2024-12-05 出版日期:2025-06-20 发布日期:2025-07-29
通信作者: 胡林峰(1981-),男,湖北天门人,副研究员,研究方向为生物农药资源开发与利用,(E-mail)wood9818@sina.com;
张志东(1977-),男,河南永城人,研究员,博士,研究方向为特殊环境微生物及益生菌资源的挖掘和利用,(E-mail)Zhangzheedong@qq.com
作者简介:朱红霞(1982-),女,山西汾阳人,助理研究员,研究方向为微生物资源挖掘与利用,(E-mail)zhxhg105@163.com
基金资助:
新疆维吾尔自治区科技创新科技援疆项目(2024E02002);新疆农业科学院稳定支持项目(xjnkywdzc-2024001-04);新疆农业科学院稳定支持项目(xjnkywdzc-2024003-70)

Expression analysis of disease resistance-related genes in wheat induced by Streptomyces sp. HU2014

ZHU Hongxia¹(), HU Linfeng²(), WANG Qiqi¹, ZHANG Jungao¹, ZHANG Shaomin¹, ZHOU Xiaoyun¹, LEI Bin², ZHANG Zhidong³()

1. Institute of Agricultural Resources and Environment / Xinjiang crop chemical control engineering technology research center, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China
2. Pesticide Trial Production Center, Xinjiang Academy of Agricultural Sciences/Xinjiang Crop Chemical Control Engineering Technology Research Center, Urumqi 830091, China
3. Institute of Applied Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China

Received:2024-12-05 Published:2025-06-20 Online:2025-07-29
Supported by:
S & T Assisting Xinjiang Project of Xinjiang Uygur Autonomous Region(2024E02002);The Stable Support Project to Agricultural Sci-Tech Renovation of Xinjiang Academy of Agricultural Sciences(xjnkywdzc-2024001-04);The Stable Support Project to Agricultural Sci-Tech Renovation of Xinjiang Academy of Agricultural Sciences(xjnkywdzc-2024003-70)

摘要/Abstract

摘要：

【目的】研究链霉菌HU2014处理对小麦抗性基因表达的动态规律,探讨该菌株诱导寄主植物产生抗病性的可能机制。【方法】采用链霉菌HU2014无细胞发酵滤液对矮抗58(AK58)、百农307(BN307)和周麦22(ZM22)等3个小麦品种进行灌根处理,分别于灌根后2、6、12、24、48和96 h采集叶片,利用实时荧光定量PCR技术检测小麦叶片中相关抗性基因表达量的变化。【结果】 HU2014菌株无细胞发酵液能够诱导4个防卫反应基因、1个水杨酸途径基因和1个茉莉酸途径基因的表达。其中TaPR1基因在处理AK58品种6 h后,BN307品种24 h后和ZM22样品48 h后表达量最大;TaPR2基因的表达在处理AK58的6 h后,BN307的12 h后和ZM22的24 h后达到最大水平;TaPR3基因表达量在AK58的2 h后,BN307的6 h后和ZM22的2 h后达到峰值;TaPR5基因在AK58的72 h后、BN307的96 h后和ZM22的12 h后达到峰值;PAL基因在AK58的48 h后、BN307的24 h后和ZM22的24 h后显示出最高的倍数变化;LOX基因表达在AK58、BN307和ZM22的6 h后达到峰值。【结论】TaPR1基因在AK58和ZM22中下调,TaPR3基因在AK58和BN307中下调。ZM22中TaPR2、TaPR3、TaPR5、PAL和LOX的最高表达水平高于AK58和BN307,而BN307中TaPR1的最高表达水平高于AK58和ZM22。链霉菌HU2014可以诱导小麦系统性获得抗性和诱导性系统抗性。

关键词: 链霉菌; 小麦; 抗病相关基因; 生物防治; 诱导抗病性

Abstract:

【Objective】 The purpose of this study is to reveal the dynamic changes in the expression of wheat resistance genes under the treatment of Streptomyces sp. HU2014 and to explore the possible mechanism by which this strain induces disease resistance in the host. 【Methods】 In this paper, the cell-free fermentation filtrate of this strain was used to irrigate the roots of three wheat varieties Aikang 58 (AK58), Bainong 307 (BN307), and Zhoumai 22 (ZM22). After irrigation, leaves were collected at 2 h, 6 h, 12 h, 24 h, 48 h, and 96 h, and the changes in the expression of related resistance genes were detected by real-time fluorescent quantitative PCR technology. 【Results】 The fermentation filtrate of this strain could induce the expression of 4 defense response genes, 1 salicylic acid pathway gene, and 1 jasmonic acid pathway gene. Among them, the transcript of TaPR1 gene accumulated at highest levels at 6 h in AK58, 24 h in BN307, and 48 h in ZM22 respectively. The expression of TaPR2 gene reached the highest level at 6 hours in AK58, 12 h in BN307, and 24 h in ZM22 respectively. The transcript of TaPR3 gene reached its peak at 2 h in AK58, 6 h in BN307, and 2 h in ZM22 respectively. TaPR5 gene reached its peak at 72 h in AK58, 96 h in BN307, and 12 h in ZM22 respectively. The PAL gene showed the highest fold change at 48 h in AK58, 24 h in BN307, and 24 h in ZM22 respectively. The expression of LOX gene reached its peak at 6 h in AK58, BN307, and ZM22 respectively. 【Conclusion】 The results indicate that the TaPR1 gene is down-regulated in AK58 and ZM22, and the TaPR3 gene is down-regulated in AK58 and BN307. The highest expression levels of TaPR2, TaPR3, TaPR5, PAL, and LOX in ZM22 are higher than those in AK58 and BN307, while the highest expression level of TaPR1 in BN307 is higher than that in AK58 and ZM22. Streptomyces sp. HU2014 can induce systemic acquired resistance and systemic resistance in wheat.

Key words: streptomyces; wheat; disease resistance related genes; biological control; inducing resistance

中图分类号:

S182

朱红霞, 胡林峰, 王琦琦, 张军高, 张少民, 周小云, 雷斌, 张志东. 链霉菌HU2014诱导小麦抗性基因的表达[J]. 新疆农业科学, 2025, 62(6): 1337-1343.

ZHU Hongxia, HU Linfeng, WANG Qiqi, ZHANG Jungao, ZHANG Shaomin, ZHOU Xiaoyun, LEI Bin, ZHANG Zhidong. Expression analysis of disease resistance-related genes in wheat induced by Streptomyces sp. HU2014[J]. Xinjiang Agricultural Sciences, 2025, 62(6): 1337-1343.

图/表 4

表1 定量PCR引物序列

Tab.1 Quantitative PCR primer sequence used in this study

引物 Gene name	上下游引物 Forward and reverse primers (5'-3')
TaActin	GGACCTCACGGATAATCTAATG TGACCATCAGGCATCTCA
TaPR1	AACCTCGGCGTCTTCATCA TTTACTCGCTCGGTCCCTCT
TaPR2TGCCGTTGCTCTCTTCAT ATGCCCTTGGACCTGTAGA
TaPR3	AGAGATAAGCAAGGCCACGTC GGTTGCTCACCAGGTCCTTC
TaPR5	ACAGCTACGCCAAGGACGAC CGCGTCCTAATCTAAGGGCAG
PAL	CCAATGTTCTGTCCGTCCT GCTGCTTCAATCTGTCCAG
LOX	AGGCAACTACATCTACGCTTC GCCATCAACACCAGAGTCA

图1 链霉菌HU2014对小麦AK58的抗性基因表达

Fig.1 The strain HU2014 on the expression of resistance genes in wheat (cultivar AK58) plant

图2 链霉菌HU2014对小麦BN307的抗性基因表达

Fig.2 The strain HU2014 on the expression of resistance genes in wheat (cultivar BN307) plant

图3 链霉菌HU2014对小麦ZM22的抗性基因表达

Fig.3 The strain HU2014 on the expression of resistance genes in wheat (cultivar ZM22) plant

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链霉菌HU2014诱导小麦抗性基因的表达

Expression analysis of disease resistance-related genes in wheat induced by Streptomyces sp. HU2014

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