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Crop Genetics and Breeding·Cultivation Physiology·Physiology and Biochemistry
Comparative study on the development of cotton bolls between Gossypium barbadense L. and Gossypium hirsutum L. in Southern Xinjiang
SHAO Yongjie, ZHAO Yuling, XIE Zongming, HE Liangrong
2025, 62(3): 521-530.  DOI: 10.6048/j.issn.1001-4330.2025.03.001
Abstract ( 61 )   HTML ( 12 )   PDF (1213KB) ( 26 )  

Objective】To study and compare the dynamic changes of boll components in the development of cotton bolls and to explore the dynamic development law of Gossypium barbadense L. and Gossypium hirsutum L. in southern Xinjiang in the hope of providing scientific theoretical basis for the management of high-yield and high-quality cultivation and high-yield and high-quality breeding of cotton.【Methods】The bolls of Xinhai No.25 and Xinluzhong No.37 were taken as the research objects, and the external traits of boll development and dry weight of boll components were compared, and besides, logistic fitting was also carried out for comparison. 【Results】External traits: the length, diameter and volume of the bolls reached their maximum values 9, 9 and 15 d later than those of land cotton, and were 15.539 mm, 7.167 mm and 3.4 cm3 less, respectively; the length-to-diameter ratio of island cotton was 0.725 more than that of land cotton; the bolls of island cotton had 3-4 chambers and 20 seeds; the chambers of land cotton had 4-5 chambers and 36 seeds. Boll traits: the dry weight of the boll shells, seeds and fibres of sea island cotton reached its maximum value 12, 9 and 3 d later than that of terrestrial cotton, and 0.419, 0.654 and 1.204 g lighter; the ratio of boll shells∶seeds∶fibres was 29∶46∶25 for sea island cotton, and 24∶43∶33 for terrestrial cotton; the dry matter of each component of the bolls of sea island and terrestrial cotton was fitted with the Logistic equation, and both showed the same dry matter as that of the land. Fitting results were "S" curve, island cotton and land cotton boll fresh boll weight, boll dry weight, volume with boll age change trends were all similar; Island cotton boll length, boll diameter and cotton seed dry weight entered and ended the vigorous growth time 1-4 d later than terrestrial cotton.【Conclusion】The single boll weight, boll chamber and cotton seed number of upland cotton are higher than that of island cotton, the boll period is shorter than that of island cotton, and the proportion of boll shell and cottonseed is lower than that of island cotton, which shows the characteristics of high lint content and significant single boll.

Study on the relationship between plant water content and morphological characteristics of top stem and leaf during the whole growth period of cotton
ZHANG Lingjian, ZHANG Kai, ZHANG Hui, GUO Xiaomeng, CHEN Guoyue, WANG Yiding, JIA Qingyu
2025, 62(3): 531-538.  DOI: 10.6048/j.issn.1001-4330.2025.03.002
Abstract ( 47 )   HTML ( 6 )   PDF (1299KB) ( 17 )  

Objective】To clarify the relationship between water status of cotton plants and morphological characteristics of top stems and leaves in the hope of providing a scientific basis for the identification, modeling and non-destructive diagnosis of cotton water characteristics. 【Methods】Using the method of field experiment, referring to the local field cotton conventional irrigation cycle and irrigation time, in the bud stage, early flowering stage, early flowering and boll-setting stage, middle flowering and boll-setting stage, late flowering and boll-setting stage, the morphological characteristics of cotton (the distance between the top two leaves, the angle between the petiole and the main stem, the angle between the leaf and the main stem) and the variation characteristics of plant water content in different growth stages were observed. 【Results】(1) In an irrigation cycle of bud stage and early flowering stage, with the decrease of plant water content, the distance between the top two leaves gradually increased, and the angle between leaf and main stem and the angle between petiole and main stem gradually decreased. (2) In the three irrigation cycles of early flowering and boll-setting stage, middle flowering and boll-setting stage and late flowering and boll-setting stage, the distance between the top two leaves and the angle between leaf and main stem increased with the decrease of plant water content, and the angle between petiole and main stem decreased with the decrease of plant water content. The distance between the top two leaves and the angle between leaf and main stem were similar to those at the initial flowering stage, while the angle between leaf and main stem was opposite to that at the initial flowering stage. (3) There was a significant linear relationship between the distance between the top two leaves, the angle between the leaf and the main stem, the angle between the petiole and the main stem and the water content of cotton. 【Conclusion】In summary, during the whole growth period of cotton, the morphological characteristics of cotton, such as the distance between the top two leaves, the angle between petiole and main stem, and the angle between leaf and main stem, have a good response to the water condition of cotton.

Effects of high-strength mulch on soil temperature, humidity and cotton growth
ZHANG Lian, CHEN Xiangyao, WANG Tangang, MA Xiaomei, CHENG Bin, WANG Gang, DUAN Zhenyu
2025, 62(3): 539-545.  DOI: 10.6048/j.issn.1001-4330.2025.03.003
Abstract ( 42 )   HTML ( 9 )   PDF (892KB) ( 9 )  

Objective】The objective of this study is to study the effects of high-strength plastic film on soil temperature, humidity and cotton growth in cotton field. 【Methods】In this experiment, two treatments, high-strength plastic film (H) and ordinary plastic film (T), were carried out in the drip irrigation cotton field in Tumshuk City, Xinjiang, and the effects of the two kinds of plastic film mulching on soil temperature, humidity and cotton agronomic and economic traits of cotton field were statistically analyzed, and the residual plastic film after use was recovered and compared. 【Results】Compared with ordinary mulch film, the high-strength plastic film warmed the 0-5cm soil by 1.72%-8.7%, and the soil at the 5-10cm layer by 1.24%-3.1%, and the warming and moisture retention effect was better in the early growth stage of cotton. Compared with the ordinary plastic film T treatment, the number of leaves increased by 1.04-1.54, the plant height increased by 1.5-7.2cm, the number of buds increased by 0.96-1.13, the yield of high-strength plastic film increased by 5.5% compared with ordinary plastic film, and the recovery rate of residual plastic film of high-strength plastic film increased by 18.86% compared with ordinary plastic film. 【Conclusion】Compared with ordinary plastic film, high-strength plastic film has better warming and moisture preservation effect, and high-strength plastic film treatment is conducive to the growth and development of cotton roots, stems, leaves and other organs, and at the same time improves the yield of seed cotton, and has high recovery efficiency.

Effects of different row spacing and defoliant on cotton defoliation
XU Shouzhen, MA Qi, NING Xinzhu, LI Jilian, SU Junji, HAN Huanyong, WANG Fangyong, LIN Hai
2025, 62(3): 546-555.  DOI: 10.6048/j.issn.1001-4330.2025.03.004
Abstract ( 39 )   HTML ( 3 )   PDF (1315KB) ( 7 )  

Objective】To reveal the physiological and ecological mechanism of the synergistic improvement of cotton plant row spacing configuration and defoliation ripening effect, reduce machine-picked impurities and improve the quality of raw cotton. 【Methods】Jinken 1643 was used as the experimental material, and the main row spacing configuration and defoliant in the northern Xinjiang cotton area were selected. The two-factor split-plot design was used in the project and the main area was the row spacing configuration, which was P6: Wide-narrow row-spacing configuration, the row spacing configuration was 66 cm + 10 cm and P3: Uniform row-spacing configuration, the row spacing configuration was 76 cm; the sub-area was sprayed with different defoliants, which were TR: Ruituolong (80% thidiazuron) and T : Xinthili (thidiazuron · ethephon suspension). By measuring the defoliation rate, miscellaneous leaf rate, boll dehydration rate, cotton boll opening rate and fiber quality of cotton plants under different treatment combinations, the effect of row spacing configuration on defoliation and ripening of cotton spraying different defoliants was discussed. 【Results】The main time period for the efficacy of the defoliant was 0-15 days after spraying the agent. The defoliation and boll opening effect of Xinthili on cotton was more rapid (0-10 d), and the defoliation rate and defoliation rate were high, the dehydration rate of cotton boll was high, and the net boll opening rate was high under the treatment of one film and six lines, which could rapidly promote defoliation and boll opening, but it would cause a significant increase in miscellaneous leaf rate and damage to the length of upper cotton boll fiber. However, under the treatment of one film and three lines, the effect of spraying Xinthili on defoliation and boll opening was poor. The defoliation and boll opening effect of Ruituolong on cotton was weak at the initial stage (0-5 d), and it mainly played a role after application (5-15 d). Although the defoliation rate and boll opening rate of cotton were slow under the treatment of one film and three lines, the final defoliation rate was not significantly different from other treatments, and it would not cause damage to cotton fiber length and increase of miscellaneous leaf rate. But the effect of spraying Ruituolong on defoliation and boll opening was poor under the treatment of one film and six lines. 【Conclusion】In summary, Xinseli has better and faster defoliation and boll opening under the configuration of one film and six rows, but it would cause the increase of raw cotton leaf impurities and the decrease of upper cotton boll fiber quality. Under the configuration of one film and three lines, the defoliation and boll opening effect of Ruituolong is relatively slow, but the final defoliation and boll opening effect is still high, and it would not cause the increase of leaf impurities and the decrease of fiber quality.

Effects of different carbon source inputs on the characteristics of compacted clay and the growth of cotton seedlings
ZHAO Yupeng, CHEN Bolang, WANG Zhiguo, FU Yanbo, BIAN Qingyong
2025, 62(3): 556-571.  DOI: 10.6048/j.issn.1001-4330.2025.03.005
Abstract ( 39 )   HTML ( 3 )   PDF (1730KB) ( 9 )  

Objective】Soil compaction and caking can lead to a significant decrease in soil quality in agricultural farmland, and a serious lack of comprehensive agricultural production capacity. Carbon source materials may have the ability to improve the structure of clayey soil. 【Methods】By using field experiments, a control group (CK), farmyard fertilizer (N group), biochar (T group), biological bacterial fertilizer (B group), commercial organic fertilizer (J group), and mineral potassium humate (H group) were designed, with three gradient treatments in each group. The aim was to explore the effects of five carbon source substances on the physicochemical properties of clayey soil and plant agronomic traits. 【Results】(1) The input of five carbon source substances had a significant impact on soil bulk density and soil porosity, with biochar having the most significant improvement effect. (2) The input of five carbon source substances had a certain improvement effect on soil aggregates of various particle sizes. Among the 1-2 mm and 1mm soil aggregates, T2 treatment had the best improvement effect, N3 treatment had the best improvement effect on 0.5-1 mm soil aggregates, and J3 treatment has the best improvement effect on 0.1-0.25 mm soil aggregates; Compared to CK, it increased by 455.70%, 504.01%, 207.41%, 65.55%, and 41.70% respectively; (3) The input of five carbon source substances could significantly improve the plant height and stem diameter of cotton plants. In terms of the improvement effect on plant height and stem diameter, the pattern presented was N>T>S>J>H and S>J>T>N>H; (4) The five carbon source substances had a significant effect on improving the dry and fresh weight of plants, and the effect of farmyard fertilizer was the best in improving the agronomic properties of cotton. 【Conclusion】In the overall evaluation, different carbon source inputs are beneficial for the physicochemical properties and plant agronomic traits of clayey soil, and the recommended amount of farmyard fertilizer+50% is the best. In summary, adding carbon source substances to clay soil can promote the formation of soil aggregates and alleviate the stress of compaction on soil by regulating soil structure and biochemical organic matter.

Effects of “dry sowing and wet emergence” on water and salt distribution, nutrients and cotton yield in different salinized soils
JIAO Runxing, BU Dongsheng, SHAO Yanhui, ZHANG Tao, CHEN Ling, ZHANG Dongdong
2025, 62(3): 572-583.  DOI: 10.6048/j.issn.1001-4330.2025.03.006
Abstract ( 37 )   HTML ( 6 )   PDF (2087KB) ( 17 )  

Objective】To investigate the effects of dry sowing and wet emergence on water and salt distribution, nutrients and cotton yield in different salinized soils.【Methods】Three cotton fields with light, moderate and severe salinity degrees were selected to dynamically monitor the water and salt changes in different locations of the seed holes, wide rows and membranes within 30 days of the seedling emergence water drips, and the cotton growth indexes, indicators of soil nutrients, salinity, pH content, etc., and the yields were analyzed during the critical period of growth and development.【Results】The overall trend of soil volumetric water content, seed hole soil conductivity and soil salinity was: heavy > medium > light cotton field, 0-20 cm soil layer of light, medium and heavy cotton field soil salinity between the membrane was generally greater than the seed holes and the wide rows; light and medium cotton field soil quick-acting nutrient content of 0-20 cm > 20-40 cm, soil alkaline dissolved nitrogen and quick-acting potassium content of the seed holes of the heavy cotton field showed an opposite trend; The soil pH of heavy cotton fields was basically below 8, and light cotton fields were above 8; the highest seedling emergence rate of light cotton fields was 83.4%, which increased by 4.51%, 5.44% and 6.59% compared with light (conventional), medium and heavy cotton fields respectively; the highest seed cotton yield of medium cotton fields was 513.5 kg/667m2, which increased by 0.51%, 37.0% and 15.0% compared with light, light (conventional) and heavy cotton fields respectively.【Conclusion】There is no obvious difference in cotton field emergence rates of different saline-alkali levels, but the high moisture content of soil volume is not conducive to cotton emergence. The moderate cotton field seed yield is the highest. The soil salt below 4 g/kg has little impact on seed germination and cotton seedling growth, but the salt content above 6g/kg will affect the yield of seed cotton.

Effects of different water and nitrogen treatments on the growth, water and nitrogen use efficiency and yield of ultra-late sowing winter wheat
NIE Lingfan, ZHANG Jinshan, TIAN Wenqiang, SUN Ganggang, WANG Hongyi, ZHANG Jun, ZHANG Qiangbin, GUO Fei, WU Li, SHI Shubing
2025, 62(3): 584-592.  DOI: 10.6048/j.issn.1001-4330.2025.03.007
Abstract ( 38 )   HTML ( 6 )   PDF (1386KB) ( 11 )  

Objective】To explore the optimal ratio of water and fertilizer for ultra-late sowing wheat in northern Xinjiang and solve the problems of low yield and low utilization rate of water and fertilizer resources for ultra-late sowing wheat in local agricultural production. 【Methods】With the local main plant Xindong 18 as the test material, three irrigation treatments and four urea treatments were wet up: W1 (2,750 m3/hm3), W2 (3,750 m3/hm3), W3 (4,750 m3/hm3), N0 (0 kg / hm3), N1 (180 kg/hm3), N2 (360 kg/hm3), N3 (5,400 kg/hm3), to analyze the effects of different water and nitrogen transportation on wheat growth, water and nitrogen utilization, dry matter accumulation and yield.【Results】The increase of irrigation volume and nitrogen application could increase wheat plant height and leaf area index. Compared with W1N0, W2N2 mature plant height increased by 13.22 cm, and the leaf area index by 3.92 m2/m2. The accumulation of dry material in wheat increased from jointing-filling-slow, and W2N2 treatment was significantly higher than those of other treatments; On the basis of W2N2, the consumption of soil water and the amount of nitrogen, and the yield was also reduced, leading to the decrease of water use efficiency. Under W2N2 treatment, the water utilization rate was 20.71% higher than that of W3N3, and the agricultural utilization rate of nitrogen fertilizer was 64.14%; the number of ear grains increased with the increase of irrigation water and nitrogen application, N2 was significantly higher than those of N0 and N1 treatments, the 1,000 grain weight increased with the increase of irrigation water, W3 treatment was significantly higher than that of W1 treatment, decreasing with the increase of nitrogen application, and reaching the maximum at W3N0. The yield of W2N2 (9,743.01 kg/hm2) was the highest and significantly higher than those of the other treatments.【Conclusion】The yield of ultra-late sowing winter wheat is 3,750 m3/hm2 with urea content 360 kg/hm2, which is the best combination of both yield and efficiency.

Effects of seed fertilizer separation on photosynthesis, dry matter accumulation and yield formation of winter wheat
SUN Ganggang, GUO Fei, NIE Lingfan, TIAN Wenqiang, WANG Hongyi, SHI Yongqing, WU Li, AI Hongyu, ZHANG Jinshan, SHI Shubing
2025, 62(3): 593-599.  DOI: 10.6048/j.issn.1001-4330.2025.03.008
Abstract ( 38 )   HTML ( 4 )   PDF (1023KB) ( 3 )  

Objective】To reveal the response mechanism of winter wheat to seed fertilizer separation spacing by studying the effects of seed fertilizer separation on the growth and development, photosynthetic characteristics, dry matter accumulation, distribution and yield formation of winter wheat in Xinjiang Tacheng area in the hope of providing technical support for the high-yield cultivation of winter wheat in Tae Basin. 【Methods】A randomized block design was conducted, and four treatments B1 (0 cm), B2 (4 cm), B3 (6 cm) and B4 (8 cm) were set up at the separation distance of seed fertilizer and then the effects of different treatments on the emergence rate, leaf area index, SPAD value, photosynthesis, dry matter accumulation, distribution and yield formation of winter wheat were studied, taking local field production as CK. 【Results】With the increase of seed fertilizer spacing in the range, wheat emergence rate, leaf area index, SPAD value, dry matter accumulation, panicle number, kernel number per spike and yield all showed an increasing trend, and B4 (8 cm) reached the maximum level. Compared with CK, the seed separation rate was significantly increased by 4.45%, panicle number increased by 4.32%, kernel number increased by 16.73% and yield increased by 5.46%. The yield was B4 > B3 > B2 > CK > B1, and the difference between B4 and B1, B2 and B3 was significant, with a significant increase of 9.35%, 3.14% and 2.25%. 【Conclusion】Adjusting the separation distance of winter wheat seed fertilizer in a certain range can improve the effect of fertilizer, increase seedling emergence rate and leaf area index, and affect winter wheat yield and yield composition. In this experimental study, B4 performs best.

Analysis of spatiotemporal variation characteristics of wheat dry hot wind disasters in Xinjiang in the past 60 years
ZHAO Yi, WU Hongqi, FAN Yanmin, SHENG Jiandong, GU Haibin, SHI Mingjie
2025, 62(3): 600-608.  DOI: 10.6048/j.issn.1001-4330.2025.03.009
Abstract ( 34 )   HTML ( 1 )   PDF (1417KB) ( 2 )  

Objective】This study aims to analyze the spatiotemporal changes of dry and hot wind disasters in Xinjiang over the past 60 years, and improve understanding of the changes in meteorological disasters in Xinjiang, in the hope of providing reference for regional large-scale dry and hot wind monitoring, warning, and defense by using daily temperature, relative humidity, and wind speed data from 103 meteorological stations in Xinjiang from 1961 to 2019.【Methods】Linear trend method was used to analyze the interannual variation characteristics and trends of Xinjiang dry hot wind disasters in time series, and Mann-Kendall mutation test method is applied to compare and analyze the mutation situation of Xinjiang dry hot wind disasters. Meanshile, inverse distance weighted (IDW) interpolation method was employed to analyze the spatial distribution characteristics of mild, moderate, and severe dry hot wind disasters in various regions of Xinjiang.【Results】Since 1960 to 2019, the dry and hot wind disasters in Xinjiang included two types: high temperature and low humidity type and dry wind type, with high temperature and low humidity type being the main disaster type; In terms of time variation, the occurrence of dry and hot winds in Xinjiang showed an overall downward trend, but the interannual fluctuation was significant, with an overall downward trend in fluctuations. Among them, the overall downward trend of high temperature and low humidity dry and hot winds was significant; The dry hot wind type showed an upward trend with significant difference; In terms of spatial variation, the spatial distribution of dry and hot winds was the highest in eastern Xinjiang, followed by northern and southern Xinjiang; The probability of high temperature and low humidity severe dry hot winds occurring in eastern and southern Xinjiang was higher than that in northern Xinjiang; Dry and hot winds mainly occurred in Turpan, Hami, and other places; Naomao Lake and Tokson were heavily affected areas by dry and hot winds.【Conclusion】The research results indicate that the number of days of dry and hot wind disasters in Xinjiang shows a decreasing trend in both time and space, but the proportion of severe high temperature and low humidity types and dry and hot wind types gradually increases, and the degree of disasters gradually worsens.

Research on virtual wheat growth simulation and visualization system based on PyOpenGL
LEI Jiacheng, ZHANG Jingjing, HAN Bo, LU Ziao
2025, 62(3): 609-618.  DOI: 10.6048/j.issn.1001-4330.2025.03.010
Abstract ( 30 )   HTML ( 2 )   PDF (1773KB) ( 5 )  

Objective】The 3D visualization function of virtual wheat growth holds significant importance for the quantitative research of wheat growth patterns.【Methods】In order to present the morphological and color changes of virtual wheat during growth, as well as its perception of lighting conditions, PyOpenGL was utilized to construct a 3D visualization system of simulated wheat growth. The system integrated morphological data with model construction, texture mapping, and lighting and shading techniques to simulate dynamic wheat growth and its field environment. The interactive and information display functions of the visual simulation system were designed by using Pyside6 user interface development tool.【Results】The study was based on the morphological characteristics of real wheat and the measured data,design the construction of the visualization system,can dynamically show the changes of wheat growth,real-time display of current wheat information description and simulation of the surrounding environment.【Conclusion】The system can intuitively and coherently present the growth process of wheat,it has a high degree of parameterization and extensibility,the corresponding growth effect can be quickly generated by modifying the basic data and parameters.

Selection and comprehensive evaluation of nitrogen-efficient foxtail millet varieties based on principal component analysis
HAO Xiyu, LIU Tingting, WANG Hui, LENG Jingwen, GONG Shihang, LIU Wei
2025, 62(3): 619-626.  DOI: 10.6048/j.issn.1001-4330.2025.03.011
Abstract ( 39 )   HTML ( 9 )   PDF (1841KB) ( 15 )  

Objective】In order to explore the effects of different organic fertilizers on fresh corn and determine suitable organic fertilizers for different planting needs. 【Methods】two varieties of fresh corn were used as the experimental materials and five different types of organic fertilizers were used as treatments. Soil fertility, agronomic traits, yield, and quality were conduct by comprehensive evaluation. 【Results】The results showed that the application of different organic fertilizers over two years led to a slow increase followed by a decrease in soil available potassium content, while other nutrient components increased annually. Amino acid organic fertilizer had the highest increase in organic matter content (0.32%), followed by microbial organic fertilizer. Chemical fertilizer had the highest increase in available nitrogen (4.75 mg/kg), available phosphorus (2.24 mg/kg), available potassium (13.55 mg/kg), total nitrogen (119.72 mg/kg), total phosphorus (33.28 mg/kg), and total potassium (1384.18 mg/kg) content, followed by amino acid organic fertilizer. Different organic fertilizers had varying effects on the agronomic traits of fresh corn, with the yield index of seaweed organic fertilizer combined with microbial agents being superior to chemical fertilizer. The nutrient content of amino acid organic fertilizer was generally at a higher level in the organic fertilizer treatments.【Conclusion】The results of comprehensive evaluation using membership function method showed that the combination of seaweed organic fertilizer and microbial agents was found to most effectively promote the ability of fresh corn, while amino acid organic fertilizer could maintain high yields while enhancing the nutritional content of fresh corn and soil fertility.

Effect of salt stress on soybean roots and GWAS analysis in seedling stage
LI Ming, ZHENG Huanbin, WANG Mingjing, ZHAO Beifang, ZHANG Sitong, YANG Suxin
2025, 62(3): 627-643.  DOI: 10.6048/j.issn.1001-4330.2025.03.012
Abstract ( 33 )   HTML ( 1 )   PDF (2251KB) ( 3 )  

Objective】So in view of this, our project aims to explore excellent salt tolerant soybean germplasm resources and identify stable expression sites related to salt tolerance.【Methods】In this study, 250 mmol/L NaCl solution was used to simulate the saline-stressed growth environment, and treat 301 soybean seedlings. 7 days later,five traits including total number of roots, number of root tips, total length of roots, root fresh weight,root dry weight were measured and then salt tolerance of soybean seedling was evaluated by principal component analysis(PCA), membership function analysis, and cluster analysis based on stress indices of various indicators, and finally, genome-wide association analysis(GWAS)was conducted based the root trait value under different treatment conditions.【Results】According to the strength of salt tolerance, the 301 materials could be divided into 4 categories. 4 strong salt tolerant varieties were classified as the first category, 73 salt tolerant varieties classified as the second category, 137 moderate salt tolerant varieties classified as the third category, and 87 salt sensitive varieties classified as the fourth category. GWAS showed that under normal treatment, two significant SNP loci were detected in root fresh weight, total root length, and total root number. A total of 7 SNP loci that might be associated with salt tolerance traits in soybean roots were detected under salt stress conditions, including the total number of roots and the number of root tips.【Conclusion】The phenotype of soybean root system is closely related to soybean salt tolerance, and screening sites related to salt tolerance through GWAS provides effective basis for the cultivation of new salt tolerant soybean varieties. A total of 9 significant SNP loci possibly related to salt tolerance traits in soybean roots were detected.

Effects of different types of fertilizers on peanut yield formation and nitrogen utilization in peanuts under border cultivation
LI Yuangao, YAN Yuemin, LIU Meijia, LI Xiuxia, GE Zhanchang, ZHANG Wensheng, ZHAO Tingting, WANG Jianguo, ZHANG Jialei
2025, 62(3): 644-651.  DOI: 10.6048/j.issn.1001-4330.2025.03.013
Abstract ( 33 )   HTML ( 0 )   PDF (986KB) ( 3 )  

Objective】To explore the effects of different types of fertilizers on peanut yield formation and nitrogen utilization under border cultivation. 【Methods】Field experiments were conducted to study the effects of different types of fertilizers on peanut yield, nitrogen,phosphorus, potassium, calcium nutrient accumulation and distribution, and nitrogen utilizat【Results】The results showed that the pod yield of CFG (Composite fertilizer and calcium fertilizer treatment), BBF (Biochar based fertilizer treatment), and SRF (Slow-release compound fertilizer treatment) were significantly increased by 15.0%, 7.3%, 12.9% compared to CF (Compound fertilizer treatment), respectively. Compared with CF treatment, CFG, BBF, and SRF treatments increased the dry matter accumulation of single plant by 8.2%, 7.1%, and 8.3%, respectively, and also increased the number of pods per plant and the weight of 100 pods, which was beneficial for improving the peanut harvest index. The leaf, pod and total plant nitrogen accumulation were 17.4%, 24.9%, 20.7% and 15.1%, 22.3%, 19.2% higher under CFG and SRF compared with CF, respectively. CFG had the highest total phosphorus accumulation in pods and plants, followed by SRF. There were no significant differences in stem and leaf potassium and calcium accumulation between SRF, BBF, and CFG treatments relative to CF, but potassium and calcium accumulation in pods were significantly higher than those of CF treatments, by 31.6%, 22.2%, 27.5% and 20.7%, 11.1%, 16.2%, respectively. Fertilization application reduced the distribution ratio of nitrogen, phosphorus, potassium and, calcium in roots, stems, and leaves, and significantly increased the distribution ratio of nitrogen, phosphorus potassium and calcium in pods. Compared with CF treatment, the agronomic efficiency of CFG, SRF, and BBF treatments increased by 56.4%, 27.3%, and 39.6%, respectively. 【Conclusion】The application of compound fertilizer combined with calcium magnesium phosphorus fertilizer, slow-release compound fertilizer, and biochar based fertilizer treatment can promote the absorption and accumulation of nitrogen, phosphorus, potassium and calcium in peanut roots, and also promote the distribution ratio of nitrogen, phosphorus, potassium and calcium in reproductive organs and pods, increase pod yield, nitrogen partial productivity, and nitrogen fertilizer agronomic efficiency.

Horticultural Special Local Products
Effects of ethylene response factor on the fruit softening of melon during storage
ZHENG Heyun, YAO Jun, ZHANG Cuihuan, WANG Zhiwei, GENG Xinli
2025, 62(3): 652-659.  DOI: 10.6048/j.issn.1001-4330.2025.03.014
Abstract ( 32 )   HTML ( 0 )   PDF (1202KB) ( 2 )  

Objective】To investigate the effect of ethylene factor (ERF) on the softening process of melon pulp during storage,this findings has provided reference for post-harvest storage and fresh-keeping of melon.【Methods】The effect of ethrel and 1-methylcyclopropene (1-MCP) on the hardness, protopectin (PP), and water soluble pectin (WSP), cellulose content, enzyme activity related to cell wall metabolism, and the correlation between hardness and cell wall metabolic index of ‘Xizhoumi 25’ melon fruits during postharvest storage were studied. 【Results】Melon was sensitive to both ethephon and 1-MCP treatments. The results showed that 1-MCP treatment slowed down the decrease in fruit firmness, protopectin and cellulose content, and retarded the increase in soluble pectin content. The result of ethephon treatment was opposite to that of-MCP treatment. There was a negative correlation between fruit hardness and PG and Cx activities during storage and the hardness was negatively correlated with PP and cellulose, but positively correlated with WSP.【Conclusion】1-MCP treatment can delay the post-ripening and softening of melon fruit by regulating cell wall metabolism, prolong storage time and reduce economic loss caused by softening.

Sulfur sensitivity of melon at different times
WANG Jing, WANG Huilin, XU Baolin, WANG Zikun, YIN Jie
2025, 62(3): 660-668.  DOI: 10.6048/j.issn.1001-4330.2025.03.015
Abstract ( 32 )   HTML ( 1 )   PDF (980KB) ( 4 )  

Objective】In order to investigate the degree of differences in sulfur sensitivity of melons in different periods, M446 (a high-generation self-compatible line) and DM18 (a high-generation self-compatible line) were used as experimental materials, and a preliminary study was carried out to investigate the influence of the growth effect of melons by spraying sulfur suspension 250 times at different periods of the melons plants. 【Methods】ANOVA, correlation analysis, principal component analysis and subordinate function analysis were used to determine the growth indexes and to evaluate melon materials M446 and DM18 comprehensively. 【Results】Sulfur spraying at different times had different degrees of inhibition on the growth indexes of melons, and the degree of sulfur sensitivity of different melon materials varied to a certain extent; all growth indexes showed a decreasing trend with the increase of the growth period, and the rate of diseased leaves and the sulfur damage index showed an increasing trend with the increase of the growth period. 【Conclusion】The degree of sulfur sensitivity of melon is summarized as fruiting period > vining period > seedling period.

Analysis of sugar and acid components and contents in different varieties of apricot fruits
MA Xintong, QIN Lihuan, Sumirikezi Kaisaer, ZHANG Dahai, Gulimire Kasimu, Waili Kadier, ZENG Bin, XIE Hui
2025, 62(3): 669-677.  DOI: 10.6048/j.issn.1001-4330.2025.03.016
Abstract ( 30 )   HTML ( 0 )   PDF (1906KB) ( 2 )  

Objective】To analyze the contents and components of main soluble sugars and organic acids in apricot fruits of different varieties, so as to provide the basis for apricot fruit quality evaluation and varieties improvement.【Methods】The contents and components of soluble sugar and organic acid in two Xinjiang apricot varieties in different developmental stages were determined by high performance liquid chromatography (HPLC), and the changes of their components and contents in different developmental stages were analyzed. 【Results】Three main soluble sugars, fructose, glucose and sucrose, were detected in Xinjiang apricot fruit and their contents of three kinds of soluble sugars and total sugars showed an increasing trend during fruit development. In the early stage of fruit development, that was, from the fruitlet to the hardcore stage, glucose was the most important soluble sugar, accounting for 62.4% to 72.8% of the total sugar. With the development of fruit, glucose content began to decrease, while sucrose content obviously started to increase in the turn to full-ripe stage, accounting for 1/3 of the total sugar. The fructose content of different varieties began to increase in the middle and late stages of fruit development. At fruit ripening, glucose and sucrose become the main soluble sugars, accounting for 78.7%-87.3% of the total sugars. The accumulation mode of soluble sugar changed from glucose accumulation mode to glucose and sucrose accumulation mode. Four organic acids, malic acid, citric acid, quinic acid and tartaric acid, were detected in Xinjiang apricot fruit. At ripening stage, the main organic acids in fruit were malic acid, citric acid and quinic acid, accounting for 82.6%-99.8% of the total acids. In the whole development stage, malic acid and tartaric acid showed a downward trend, while citric acid and quinic acid showed an upward trend. From the fruitlet stage to the enlargement stage, both of the varieties were dominated by malic acid and quinic acid. Fromf the color conversion stage of Chang saimaiti, the content of quinic acid increased rapidly, the content of malic acid decreased, and gradually changed from malic acid to quinic acid. The malic acid content of Yilangtumuxiuke decreased rapidly, the citric acid increased rapidly, the tartaric acid decreased gradually, and the quinic acid did not change significantly.【Conclusion】In the later stage of fruit development, soluble sugars change from glucose to sucrose and glucose, organic acids change from malic acid to malic acid and quinic acid and citric acid and quinic acid. Quinic acid has little effect on fruit acidity. The soluble sugars and organic acids of different apricot varieties show significant differences in the whole process of fruit development. The difference of sugar and acid components and content cause the difference of taste of apricot fruits of different varieties.

Cultivation characteristics and berry quality analysis of twelve grape cultivars in Turpan grape production area
BAI Shijian, HU Jinge, CAI Junshe, WU Jiuyun, MA Xiaocai, YUAN Sen, WU Guohong
2025, 62(3): 678-687.  DOI: 10.6048/j.issn.1001-4330.2025.03.017
Abstract ( 35 )   HTML ( 1 )   PDF (1366KB) ( 4 )  

Objective】This study aims to select suitable grape varieties for Turpan producing area and optimize the grape cultivars structure in the production area. 【Methods】Twelve grape cultivars, including Xinyu, Xinya, Qiuhongbao, Shennongjinhuanghou, Guifeimeigui, Zixiameigui, Lvzhoubaoshi, Huozhouziyu, Huozhoucuiyu, Zitianwuhe, Zicuiwuhe, and Lihongbao were used as materials. Cultivation characteristics and berry quality were compared by field investigations and laboratory analysis method in open field cultivation, and berry quality indexes were analyzed by principal component analysis.【Results】There were differences in phenology, growth and fruiting habits, resistance to powdery mildew and berry quality among the twelve grape cultivars. Shennongjinhuanghou and Guifeimeigui were early maturing cultivars, Xinyu and Xinya were medium maturing cultivars; Huozhoucuiyu and Huozhouziyu were late maturing cultivars, others were mid-late maturing cultivars. Xinyu, Xinya, Qiuhongbao, Huozhouziyu and Zitianwuhe had high yield with the mean yields per plant were 21.13, 30.70, 20.59, 18.22 and 25.70 kg, respectively, and all had strong or moderate growth potential. Guifeimeigui, Qiuhongbao, Huozhoucui and Xinyu were susceptible to powdery mildew, other cultivars were resistant. The cluster mass of Xinyu grape (1,243.11 g) and berry mass (14.12 g) were both the highest, soluble solid content of Guifeimeigui was the highest (22.56 ° Brix), while other cultivars had soluble solid content ranging from 18.15 to 19.82 ° Brix; Lvzhoubaoshi and Huozhoucuiyu had higher titratable acid content than other grapes, 0.55% and 0.54%, respectively. The edible rates of seeded grape cultivars Xinyu and Xinya were relatively high, 98.81% and 98.62%, respectively. The berry quality of the twelve grape cultivars was ranked as follows: Xinyu > Zitianwuhe > Xinya > Qiuhongbao > Zicuiwuhe > Guifeimeigui> Huozhouziyu > Shennongjinhuanghou > Lihongbao > Huozhoucuiyu > Zixiameigui > Lvzhoubaoshi. Cluster analysis showed that twelve grape cultivars could be divided into five categories, each type of grape had its typical characteristics. 【Conclusion】The cultivation characteristics and berry quality of five grape varieties: Xinyu, Xinya, Zitianwuhe, Qiuhongbao and Huozhouziyu are superior to other cultivars, which are suitable for promotion and planting in Turpan area.

Control of Plodia interpunctella by hypobaric treatment combined with sulfur dioxide (SO2) fumigation on dried apricot
ZHANG Hao, ZHANG Zhen, YUAN Yuyao, WANG Chengwei, ZHAO Zhengyu, WEI Jia, WU Bin
2025, 62(3): 688-695.  DOI: 10.6048/j.issn.1001-4330.2025.03.018
Abstract ( 29 )   HTML ( 1 )   PDF (978KB) ( 2 )  

Objective】This study aims to enhance the storage and transportation quality of Xinjiang dried apricots, thus ensuring food safety during processing and minimizing losses from insect pests and economic losses in the industry by applying hypobaric treatment combined with SO2 fumigation to manage insect pests in order to reduce SO2 residues and mitigate storage losses of dried apricots.【Methods】The efficacy of hypobaric treatment combined with SO2 fumigation was evaluated using different SO2 concentrations (250, 500, 1000 μL/L) and six C×T values (0, 2,000, 4,000, 6,000, 8,000, 10,000 μL/(L·h)). The control effects on eggs, larvae, pupae, and adults of Plodia interpunctella in dried apricots were evaluated, along with changes in dried apricots quality.【Results】When the C×T value reached to 10,000 μL/(L·h), the mortality rate of Plodia interpunctella in different life stages reached 100%. Analysis of LT50 and LT95 values revealed the sensitivity order to hypobaric treatment combined with SO2 fumigation: adult > egg > pupa > larva. This method effectively reduced SO2 residues, improved food safety, and maintained sensory quality of dried apricots.【Conclusion】Hypobaric treatment combined with SO2 fumigation shows promise in preventing and controlling Plodia interpunctella infestations and reducing SO2 residues in dried apricots.

Plant Protection·Soil Fertilizer·Microbes
Behavioral responses of Orchestes steppensis to five species elm leaves and initial exploration of active volatiles of host plants
YANG Meng, LU Zihan, ZHANG Ya, XUE Xiaomeng, ZHONG Wen, PENG Zeming, LI Qin
2025, 62(3): 696-705.  DOI: 10.6048/j.issn.1001-4330.2025.03.019
Abstract ( 38 )   HTML ( 5 )   PDF (1151KB) ( 5 )  

Objective】This study aims to investigate the selective behavioral response of Orchestes steppensis to five Ulmus (elm) leaf species and analyze the volatile composition of these leaves. The goal is to find out whether plant volatiles are responsible for the selection preference of O. steppensis for five species of elm. 【Methods】Air was used as a blank control odor source, and the behavioral responses of air-U. pumila, air-U. pumila L. cv. 'Tenue', air-U. davidiana ‘Changzhi’, air-U. densa, and air-U. laciniata were determined using a four-armed olfactometer. On this basis, the behavioral responses of O. steppensis to the above selected elm leaves were determined using a four-armed olfactometer, and the number of males and females arriving at the odor source vials and the time of arrival were recorded to compare the selective preferences of O. steppensis for the leaves of the five species of elm. The volatile components of the five species of elm leaves were detected by solid-phase microextraction combined with gas chromatography-mass spectrometry.【ResultsO. steppensis showed no selective behavior for U. laciniata and chose the remaining four elm species, but there was a selective preference, preferring U. pumila, followed by U. pumila L. cv. 'Tenue', then U. densa, and finally U. davidiana. The number of choices made by females for U. pumila, U. pumila L. cv. 'Tenue', and U. densa were all highly significantly higher than those made by males, while there was no significant difference between the number of choices made by male and female adults for U. davidiana. In terms of reaction time, O. steppensis females took the longest time to reach U. davidiana, which was significantly higher than those of U. densa, U. pumila, and U. pumila L. cv. 'Tenue', while males took longer time to reach U. davidiana and U. densa, which was significantly higher than that of U. pumila and U. pumila L. cv. 'Tenue'. The results of the GC-MS analyses showed that a total of 79 compounds were identified from five elm leaf volatiles, and 20, 18, 31, 23 and 10 compounds were detected respectively in U. pumila, U. pumila L. cv. 'Tenue', U. davidiana, U. densa, and U. laciniata leaf volatiles. 【Conclusion】Elm leaf volatiles are responsible for O. steppensis feeding preferences on five species of elm.

Changes in bacterial diversity and community structure in rhizosphere soil of lavender with different continuous cropping years
ZHANG Xiaole, Wueren Ahebieerdi, Entemake Bulatibai, Muguli Muhaxi, YUAN Qingqing, BAI Ru
2025, 62(3): 706-714.  DOI: 10.6048/j.issn.1001-4330.2025.03.020
Abstract ( 36 )   HTML ( 4 )   PDF (1195KB) ( 5 )  

Objective】To explore the structure and diversity characteristics of bacterial communities in the rhizosphere soil of lavender with different continuous cropping years, as well as their evolutionary patterns.This article provides reference for the scientific management and growth and yield increase of lavender soil.【Methods】Three sets of soil samples were collected from Huocheng County, Ili Kazakh Autonomous Prefecture, with different crop continuously years and without lavender planted. The 16S rDNA sequence was subjected to high-throughput Illumina sequencing. Afterwards, the sequencing results were analyzed, the bacterial diversity and community distribution patterns of each sample group were compared to find their correlation with planting years.【Results】Alpha diversity analysis showed that with the increase of continuous cropping years, both Shannon index and Chao1 index first decreased and then increased. At the phylum level, Acidobacteria, Proteobacteria, and Actinobacteria were dominant phyla. At the genus level, the dominant bacterial genera were an unclassified genus in the phylum Acidobacteria, the genus Sphingomonas, and the genus Acidophilus. The bacterial community composition of lavender soil with different ages was similar, but the relative abundance varied. Beta diversity also showed significant changes compared to unplanted soil, with the first and fifth years being similar, and both showing significant differences compared to those of the third year.【Conclusion】The continuous cultivation of lavender causes changes in the bacterial community structure and diversity of rhizosphere soil.

Multimethod screening of bacterial diversity and related strains in radiation-contaminated areas
XIE Wenwen, WANG Huinan, Ainijiang Ersiman, ZHU Jing, GU Meiying, ZHANG Zhidong
2025, 62(3): 715-722.  DOI: 10.6048/j.issn.1001-4330.2025.03.021
Abstract ( 29 )   HTML ( 0 )   PDF (1281KB) ( 6 )  

Objective】To screen and mine the microbial species resources by analyzing the diversity and community composition of soil bacteria in the radiation-contaminated area. which provides a theoretical basis for the comprehensive mining and utilization of the microbial resources in radiation-contaminated areas. 【Methods】Soil samples were randomly collected from three sites in the high radiation area of a radiation-contaminated area in northwest China, and the composition of soil bacterial communities in the area was analyzed by using Illumina NovaSeq high-throughput sequencing technology; the bacteria within the soil were isolated by various screening methods, molecularly identified by using 16S rRNA, and their functional properties were preliminarily studied. 【Results】The results of high-throughput sequencing showed that the bacterial domains within the soil samples involved 30 phyla and 384 genera, with the dominant phyla being Firmicutes (52.15%), Proteobacteria (30.17%), Bacteroidota (10.42%) and Actinobacteriota (4.94%). Actinobacteriota, 4.94%). Planococcus (17.45%), Exiguobacterium (13.65%), Bacillus (6.97%) and Pontibacter (6.78%) were the dominant genera. Culturable methods were used to isolate 277 bacterial strains which belonged to 51 genera and 97 species. The dominant bacteria genera were Microvirga (24.91%), Streptomyces (16.25%), Pontibacter (7.22%), Cellulomonas (5.78%), etc. Ten potential new species were isolated, and majority of the obtained strains had strong salinity tolerance and functional enzyme-producing activities. 【Conclusion】The diversity of soil bacteria in radiation-contaminated areas is relatively rich.

Drought resistance evaluation of 11 wild Agropyron cristatum germplasm at germination stage under PEG simulated stress
YI Fengyan, SUN Shixian, GUO Chengyu, YAN Xiaohong, FANG Yongyu, YANG Ding, DING Haijun
2025, 62(3): 723-731.  DOI: 10.6048/j.issn.1001-4330.2025.03.022
Abstract ( 27 )   HTML ( 0 )   PDF (898KB) ( 1 )  

Objective】The germplasm resources of drought tolerant Agropyron cristatum were screened by PEG simulated water stress.【Methods】The seeds of 11 A. cristatum germplasm resources collected were used as the testing materials to evaluate the effects of different Polyethylene glycol (PEG-6000) concentration on six seed germination indexes (relative germination rate, relative germination potential, relative germination index, relative vitality index, relative seedling height and relative root length), and drought resistance of these materials was was comprehensively evaluated by using the membership function method.【Results】The results showed that PEG simulated drought stress had significant effects on the germination stage of 11 A. cristatum germplasm resources, and there were significant differences among the materials, but the indexes showed a decreasing trend with the increase of PEG concentration. Low concentration of drought stress had a certain promotion on germination indexes of A1, A8 and A9, especially on RGR, RGI and RVI. The germination indexes of 11 A. cristatum germplasm resources was inhibited with the increase of PEG stress concentration, and the inhibition effect on RSH and RRL was stronger, when the concentration reached 25%, the A3 and A4 indexes were completely inhibited. Correlation analysis showed that there was a significant correlation between the six indicators, and the correlation coefficient was 0.831 to 0.981, RGP has the highest correlation with RGI. The germination indexes of 11 A. cristatum germplasm resources were evaluated by membership function method and with D value range of 0.387,7 to 0.504,5, and the drought resistance of A1 and A9 was better than those of other materials.【Conclusion】There are differences in the indexes of the germplasm resources at the germination stage of 11 A. cristatum germplasm resources under drought stress with the increase of PEG-6000 concentration, and the drought resistance is as follows: A1>A9>A6>A2>A7>A5>A8>A10>A11>A4>A3.

Effects of Sophora alopecuroides L. extracts on allelopathy of Hami melon seed germination
SHENG Yangqian, ZHOU Jiaqi, HUANG Yangjie, ZHANG Meiqi, XUE Nana
2025, 62(3): 732-738.  DOI: 10.6048/j.issn.1001-4330.2025.03.023
Abstract ( 40 )   HTML ( 5 )   PDF (760KB) ( 6 )  

Objective】To clarify the impact of Sophora alopecuroides L. extracts on allelopathy of Hami melon seed germination by investigating the interactive effects of soil extracts (continuous cropping for 2 years and 7 years) and different concentrations of Sophora alopecuroides L. (5, 25 and 50 g/L) on the seed germination of Hami melon.【Methods】The germination potential, germination rate, germination index, and allelopathy response index of the Hami melon seeds were determined using the filter paper method in Petri dishes.【Results】The continuous cropping soil extracts significantly inhibited the seed germination rate, with a decrement in germination rate of 30.56% and 41.67% respectively. Similarly, Hami melon seed germination rate decreased by 19.44%-61.11% after treatment of Sophora alopecuroides L. water extracts. However, further findings from the interaction effects of continuous cropping soil extracts and Sophora alopecuroides L. extracts on seed germination indicated that low concentration of Sophora alopecuroides L. extracts (5g/L) could clearly reduce the long-term continuous cropping barrier effect of Hami melon (7 years) compared to 2 years. 【Conclusion】Low concentration of Sophora alopecuroides L. (5 g/L) might mitigate the seed germination allelopathy caused by long-term continuous cropping of Hami melon.

Effects of desert reclamation and fertilization on soil organic carbon storage and microbial community characteristics
FU Pengyu, LIANG Meng, LI Chenhua
2025, 62(3): 739-747.  DOI: 10.6048/j.issn.1001-4330.2025.03.024
Abstract ( 34 )   HTML ( 4 )   PDF (1486KB) ( 6 )  

Objective】To study the effects of reclamation and long-term fertilization on soil organic carbon (SOC) storage and microbial community characteristics (microbial abundance and community structure) in desert soils. 【Methods】Taking the long-term positioning experiment of Fukang Desert Ecological Station of Chinese Academy of Sciences as the platform, 11 management modes were selected: no fertilization (CK [a]), single application of chemical fertilizer (PK [b], NK [c], NP [d], NPK [e], N2P2 [f], N2P2K [g]), chemical fertilizer with straw (NPKR [h], NPKR2 [i], N2P2R3 [j]) and chemical fertilizer with manure (NPKM [k]). They were compared with the adjacent native desert soil by collecting 0-20 cm soil layer samples, and then the characteristics of soil organic carbon storage and microbial community in the process of desert reclamation and the relationship between them were analyzed. 【Results】When reclaimed and long-term fertilized soils to adjacent desert soils were compared, reclamation and fertilization significantly increased soil organic carbon storage. In addition, the abundance of soil bacteria, fungi, archaea, and nitrogen-fixing microorganisms was substantially increased, with the following order of enhancement: fungi > bacteria > archaea. Specifically, treatments g, j, and k showed higher increases in both soil organic carbon storage and microbial abundance. 【Conclusion】Desert reclamation and fertilization, through reducing soil pH and salinity, increasing the input of exogenous organic matter and nutrients, have increased the storage of soil organic carbon and the abundance of microorganisms, and changed the structure of the microbial community. The positive effect of vegetation on the formation of soil organic carbon sinks in arid areas, and the importance of chemical fertilizers (especially N and P) combined with organic fertilizers for soil organic carbon storage and microbial abundance in arid regions.

Animal Husbandry Veterinarian
RT-RAA detection method based on porcine reproductive and respiratory syndrome virus Nsp2 gene
YU Na, ZHAO Aiyun, HUANG Chunyuan, MA Jiamei, ZHANG Ziwei, FAN Yuexuan, ZHENG Jiaxin, ZHANG Yan, LIU Guangliang, QI Meng, CAO Zongxi
2025, 62(3): 748-753.  DOI: 10.6048/j.issn.1001-4330.2025.03.025
Abstract ( 35 )   HTML ( 4 )   PDF (929KB) ( 20 )  

Objective】The objective of this study is to establish a rapid detection method for PRRSV Nsp2 gene by reverse transcription recombinase-mediated isothermal amplification (RT-RAA) fluorescence. 【Methods】Primers and probes of classical strains and highly virogenic strains were designed to detect the sensitivity and specificity of RT-RAA.【Results】The results showed that the proposed method had no cross-reactivity with the nucleic acids of swine fever virus (CSFV), pseudorabies virus (PRV Bartha-K61), porcine epidemic diarrhea virus (PEDV Purdue) and porcine infectious gastroenteritis virus (TGEV LJX). The minimum detection limit of this method was 1.71×101 copies/μL for highly pathogenic strains and 2.19×103 copies/μL for classical strains. Compared with the PCR method, the sensitivity and specificity of RT-RAA fluorescence quantification method were 95.5% and 100%, respectively. The sensitivity and specificity of RT-RAA classical strain fluorescence quantification method were 93.0% and 100%, respectively and the two methods were highly consistent. 【Conclusion】The PRRSV RT-RAA fluorescence method established in this study can distinguish two different strains with good specificity and high sensitivity.

Effects of fermented Chinese herbal medicines on growth performance, serum biochemistry and growth hormone in lamb
LIU Limeng, MA Wenbin, LI Lingui, YUAN Cen, SHI Zhihai, LIU Yanfeng, QIN Rongyan, WANG Wenqi
2025, 62(3): 754-765.  DOI: 10.6048/j.issn.1001-4330.2025.03.026
Abstract ( 35 )   HTML ( 1 )   PDF (1379KB) ( 5 )  

Objective】The purpose of this study is to investigate the effects of fermented Chinese herbal medicines on the growth performance, serum biochemistry and growth hormone of lambs.【Methods】A total of 120 15-day-old lambs with similar newborn weight (3.25±0.52)kg in good body condition were selected and randomly divided into 4 groups (3 replicates in each group, 10 sheep in each replicate), 10 sheep in each replicate) and fed lamb starter feed (0 groups), lamb starter feed + 0.2% fermented Chinese herbal medicine (0.2% group), 0.4% fermented Chinese herbal medicine (0.4% group) and 0.6% fermented Chinese herbal medicine (0.6% group) respectively. The test period lasted 45 days.【Results】The results showed that: 1) The body weight of the experimental group at 30 days of age was significantly higher than that of the control group (P<0.05). The 60-day-old body weight of the 0.2% and 0.6% groups was significantly higher than that of the 0 group (P<0.05). The 21-25 days of age, 26-30 days of age, and 36-40 days of age in the experimental group were significantly higher than those in the control group (P<0.01). The starter intake (SI) of the 41-45-day-old starting feed in the 0.6% group was significantly higher than that in the 0 group (P<0.01). 2) The contents of anti-tumor factor-α (TNF-α) and interleukin-6 (IL-6) in the serum of lambs in the experimental group were significantly lower than those in the control group (P<0.01). The content of protein (GLB) in the serum of lambs in the 0.6% group was significantly lower than that in the 0 group, 0.2% group and 0.4% group (P<0.05). The serum content of superoxide dismutase (SOD) in the experimental group was significantly higher than that in the control group (P<0.01). The serum levels of total antioxidant capacity (T-AOC) and glutathione peroxidase (GSH-Px) in the 0.4% and 0.6% groups were significantly higher than those in the 0 group (P<0.01). The serum levels of malondialdehyde (MDA) in the 0.4% and 0.6% groups were significantly lower than those in the 0 group (P<0.01).【Conclusion】It can be seen that the addition of fermented Chinese herbs to the diet of lambs under the conditions of this experiment can help to improve growth performance, promote the secretion level of growth hormone, increase the body's antioxidant capacity and immune level, thus alleviating the weaning stress of lambs.

Genome-wide selection signaling reveals candidate genes associated with double-coated fleece in Hotan sheep
ZHANG Yanwei, XU Xinming, YU Lijuan, TIAN Yuezhen, XIE Mengwan, TANG Liping, ZHENG Peiyu, SONG Nannan, DI Jiang
2025, 62(3): 766-774.  DOI: 10.6048/j.issn.1001-4330.2025.03.027
Abstract ( 29 )   HTML ( 0 )   PDF (1226KB) ( 1 )  

Objective】This project aims to screen out genes and pathways related to the double-coated fleece of Hotan sheep in the hope of providing a theoretical basis for further analysis of the molecular regulation mechanism ofdouble-coated fleece of the sheep.【Methods】The genome-wide SNPs derived from Hotan sheep with double-coated fleece and Chinese Merino sheep with single-coated fleece were used to determine candidate genes associated with double-coated fleece in Hotan sheep. Healthy adult female Hotan sheep and Chinese Merino sheep were selected with 15 individuals each, blood samples were collected from jugular vein, and genomic DNA was extracted for re-sequencing. Selection signal detection was performed based on whole genome SNPs, and top 1% of Fst and θπRatio value were set as thresholds. SNPs harbored in the strongly selected genomic regions were annotated, then GO function and KEGG pathway enrichment analysis were carried out to further screen out the candidate genes and biologicalpathways related to the double-coated fleece traits of Hotan sheep. 【Results】Our results showed that totally 732 selected genes were identified by Fst and θπRatio approaches, and these genes were significantly enriched into several KEGG pathways related to wool traits, even 1 biological process pathway related to hair follicle development.【Conclusion】Multiple pathways related to wool traits and five key candidate genes (BMPR2, LPAR6, RARA,CDC6 and BNC1) that may be related to Hotan sheep double-coated fleece were screened.

Analysis of calving difficulty of Chinese Simmental Cattle in Yili area
HU Zexu, WANG Chuanjun, ZHANG Xiaoxue, ZHANG Menghua, ZHANG Tao, TAN Xueting, SHANG Yanyan, MA Kailun, WANG Chengcheng, ZHANG Guixia, HUANG Xixia
2025, 62(3): 775-780.  DOI: 10.6048/j.issn.1001-4330.2025.03.028
Abstract ( 30 )   HTML ( 0 )   PDF (620KB) ( 4 )  

Objective】The calving difficulty of Simmental Cattle in China was analyzed by different parity, calving season, calf birth weight, calf sex and calving type.【Methods】Calving information of 18405 cows from 2018 to 2023 was collected from a large-scale cattle farm in Yili, Xinjiang and the least squares analysis of calving difficulty and ease factors was carried out by using SAS9.2 software.【Results】The parity had a significant effect on calving difficulty (P< 0.01). Calving season had significant effect on calving difficulty (P < 0.05). The birth weight of calves had a significant effect on calving difficulty (P< 0.01). The sex of calves had significant effect on calving difficulty (P< 0.01). Calving type (singleton and twin) had a significant effect on calving difficulty (P< 0.01).【Conclusion】The difficult childbirth rate of cows in spring and wither is higher than that in summer;Low temperatares in spring and winter make newborn calves more sensitive to harsh weather conditions;calves with lighter body weight have weaker adaptability to the external environment of the uterus conpared to calves with heavier boby weight; The difficulty of childbirth in male calves is generally higher than that in female calves.

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Recent Advances of Edible Coatings in Reservation of Fruits and Vegetables
FU Yan-yan, MEI Chuang, WANG Yan-peng, WANG Ji-xun, LU Chun-sheng, YAN Peng, ZHANG Fu-chun, FENG Bei-bei
Xinjiang Agricultural Sciences    2019, 56 (12): 2263-2274.   DOI: 10.6048/j.issn.1001-4330.2019.12.012
Abstract753)      PDF (1144KB)(5417)      
Objective】 The research progress of edible film/coating in fruits and vegetables was collected, sorted out and summarized, the effect of different coating materials was comprehensively analyzed, and the physiological indexes after harvest and storage were summarized in the hope of providing a reference for the wide range of application of edible coating in fruits and vegetables. 【Methods】By collecting and consulting relevant research literatures at home and abroad, agricultural and food official website information, combined with existing research results, we summarized and analyzed the data in the past ten years, and then we conducted statistics. 【Results】 The commonly used coatings for fruit and vegetable preservation currently included polysaccharide coatings (konjac glucomannan and chitosan), lipid coatings (wax and paraffin), protein coatings, composite coatings and other coating materials. Fruit and vegetable could enhance the activity of antioxidant enzymes, improve the antioxidant capacity and reduce the production of free radicals after coating treatment. What’s more, these treatments kept the quality and extended the storage time. For example, ‘Nanguo’ pear and strawberry treated by chitosan extended the storage period for 20 days and 12 days, respectively, and maintained the sensory quality at the same time. 【Conclusion】 At present, polysaccharide and lipid coatings are mainly used in the preservation of prosperous fruits such as blueberries, strawberries, grapes, and the fresh-cut fruits such as cantaloupe to prolong the storage period. The protein coatings are mainly used in meats (pork, fish, etc.) to maintain their quality.
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Analysis of the Microbial Community Diversity of Soil from Wine Grape Productiing Area in Xinjiang Based on High-throughput Sequencing
WANG Wei, Buligen Jialengbieke, HU Xiaodong, XIA Junfang, ZHANG Zhidong, GU Meiying, WU Yun
Xinjiang Agricultural Sciences    2020, 57 (5): 859-868.   DOI: 10.6048/j.issn.1001-4330.2020.05.009
Abstract444)      PDF (1856KB)(2604)      
【Objective】 To find out the microbial community structure and diversity in the soil of the four major wine grape producing areas in Xinjiang and to lay a foundation for constructing the microbial bank in the main wine grape producing areas in the region. 【Methods】 After total DNA was extracted, Illumina MiSeq high-throughput sequencing system and bioinformatics analysis were used for the investigation of microbial communities and species diversity of main wine grape producing area in Xinjiang. 【Results】 In the twelve soil samples, the results showed that the highest microbial diversity of bacteria was the soil in Yili river-valley and the lowest one was in the soil of northern slope of Tianshan Mountain, and the highest microbial diversity of fungus was in the soil of Yanqi basin and the lowest microbial diversity of fungus was the Tuha basin soil. The dominant bacteria and fungus were Arthrobacter and Guehomyces in Tuha basin and the northern slope of Tianshan Mountain wine grape producing area. The dominant bacteria and fungus were Gemmatimonas and Tetracladium in Yili river-valley wine grape producing area. The dominant bacteria and fungus were Arthrobacter and Gibberella in Yanqi basin wine grape producing area. Soil pH value had the most significant effect on the composition of bacterial community, and the content of Mn had the most significant effect on the composition of fungal community. 【Conclusion】 This study revealed the composition of bacterial and fungal communities in the soil in main wine grape producing areas in Xinjiang, which indicated that there were differences in microbial community diversity.
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Potential Risk Zone Analysis of Apocheima cinerarius Erschoff disaster in Xinjiang Featured Forest Fruits
WANG Lei, LUO Lei, LIU Ping, HOU Xiao-chen, QIU Qin, GAO Ya-qi, LI Xi-guang
Xinjiang Agricultural Sciences    2019, 56 (9): 1691-1700.   DOI: 10.6048/j.issn.1001-4330.2019.09.015
Abstract391)      PDF (1413KB)(2111)      
【Objective】 To analyze and predict the potential risk areas of Apocheima cinerarius Erschoff in Xinjiang fruit forests in the hope of providing important reference value for effective prevention and key prevention of Apocheima cinerarius Erschoff disaster in Xinjiang fruit forests. 【Method】Based on the actual distribution data and environmental factors data of Apocheima cinerarius Erschoff disaster in Xinjiang fruit forests, the potential risk areas of Apocheima cinerarius Erschoff in Xinjiang fruit forests were predicted by MaxEnt model and GIS spatial analysis technology, and the main environmental factors and niche parameters were analyzed by percentage contribution rate. 【Results】The results showed that: (1) The ROC evaluation (Receiver Operating Characteristic, ROC) showed that the AUC values of the training data set and the test data set of MaxEnt model predicting the potential risk area of Ectropis sinensis were 0.979 and 0.970 respectively, which indicated that the simulation effect was excellent. (2) According to the prediction results of MaxEnt model, the potential distribution area of Apocheima cinerarius Erschoff in Xinjiang fruit forests was divided into risk area and risk-free area, and the total area of risk area was about 74,538,800 hm2.(3)Based on cluster analysis, the potential risk areas of Apocheima cinerarius Erschoff in Xinjiang fruit forests were divided into high-risk areas: 1.158 million , medium-risk areas:2.218 million hm2and low-risk areas:4.226 million hm2. (4) The main environmental factors affecting the risk of Apocheima cinerarius Erschoff disaster in fruit forests in Xinjiang were the highest temperature in cold month, the average temperature in the coldest season, the precipitation in the driest season and the average temperature in the wettest season. The response curves showed that the highest temperature in the cold month > 14.25 C, the average temperature in the coldest quarter > 4.01 C, the pre-cipitation in the driest quarter was between 1.23-8.29 mm, and the average temperature in the wettest quarter was between 21.63-24.82 C, which was the niche parameter suitable for the growth of Apocheima cinerarius Erschoff in Xinjiang fruit forests and fruits. 【Conclusion】Based on MaxEnt model, the prediction results of potential risk areas of Apocheima cinerarius Erschoff in Xinjiang characteristic forests are completely consistent with the actual distribution areas of Apocheima cinerarius Erschoff. The geographical distribution characteristics of Apocheima cinerarius Erschoff are clear: the high-risk areas are mainly located in Kashgar and Hetian areas, and the middle-risk areas are mainly located in Aksu area, Bayingol Mongolian Autonomous Prefecture and Hetian area. The low-risk areas are located in Aksu area, Bayingol Mongolian Autonomous Prefecture, Hotan area, Kashgar area and Kizilesu Kirgiz Autonomous Prefecture, with a small distribution in Turpan area and Urumqi city. This study has a high reference value for formulating quarantine and prevention policies.
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The Recent Advances in Research on Carpel and Related Functional Genes in Angiosperm
Yang Jing, Wang Wei-ran, Zhu Jia-hui, Ning Xin-min, Liu Zhi-qing, Kong jie, Alifu Aierxi
Xinjiang Agricultural Sciences    2019, 56 (8): 1535-1543.   DOI: 10.6048/j.issn.1001-4330.2019.08.019
Abstract906)      PDF (1220KB)(1894)      
Objective】 Carpel is an important female organ in flowering plants, and it as a focus was researched in crop plants development. The origin of the carpel development, the function and development of the carpel, the quantity heredity of carpel and the adjust of hormone, and the gene to control of the carpel development, then the result were summarized the research progress on recent advances in china and abroad. The aim to as a guide for improvement the multi-carpel variety in crop. 【Methods】 The research progress of plant carpels that from the literature databases of china and abroad, then was consulted and analyzed. 【Result】 At present, there have two theories about the origin of carpel: one the theory of carpel and another theory of reproductive leaf; the carpel can protects ovules, receives pollen and makes some egg cells to fertilize, save and disseminates seeds; the development process of carpel is usually folded and curled at the internal space in the organs of flowers, in which the ovules are wrapped and developed. The number of carpels is increase was steady. hormone have important function and effect the carpels development. Genes play an important role in regulating the number and development of carpel. 【Conclusion】As the key of pistil development in angiosperms, carpel is very important for the formation of yield traits. In the future, the study of carpel should focus on the whole process of carpel development. It is a feasible way to identify the functional genes regulating the number and development of carpel as soon as possible, and to breed multi-carpel, high-yield and high-quality varieties through gene improvement.
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Analysis on Yield Potential and Related Agronomic Characters of New Maize Grain Mechine Harvesting Variety Xinyu 108 with Drought Tolerance
LIANG Xiao-ling, Abulaiti Abra, YANG Jie, HAN Deng-xu, WANG Ye-jian, XI Hao-jiang, LI Ming-dong, LEI Zhi-gang, GAO Xue-fei, CHEN Shao-jiang, LIU Wen-xin, DAI Jing-rui
Xinjiang Agricultural Sciences    2019, 56 (9): 1597-1608.   DOI: 10.6048/j.issn.1001-4330.2019.09.004
Abstract1156)      PDF (1054KB)(1780)      
【Objective】 To analyze the yield potential and adaptability of Xinyu108, a new direct - harvesting maize hybrid with early - mid maturity, and to provide a scientific basis for its extension. 【 Method】Based on the regional test, production test and multi - point test results of early - mid maturity spring maize sown in northern Xinjiang, RCT99 software was used for statistical analysis. 【 Result】 The average yield of the two - year regional trial was 953. 5 kg / 667 m2 , 5. 89% higher than that of the control Denghai 3672. The rate of increasing production point was 89% . The yield of production test was 907 kg / 667 m2 , which increased by 2. 97% compared with the control. The rate of increasing production point was 80% . The average output was 1,103.4 kg/667 m2, and the high-yield field production reached to 1,327 kg/667 m2.The average growth period was 120. 1 days, 0. 45 days earlier than that of the control, and the effective accumulated temperature needed was 2, 500℃ ( ≥10℃ ) . Grain bulk density was 782 g/L, crude protein 9. 6% , crude fat 3. 5% , and crude starch 75. 49% . The lodging and breakage rate was 1. 2% , the moisture content was 23. 7% , the damage rate of machine - harvested grains was 3. 78% , the ear - drop rate was 0, the grain - drop rate was 0.7% , and the yield loss rate was 0. 7% . The drought tolerance index is 0.95, which ranked strong tolerance level as same as Xianyu 335 and has two grades of drought tolerance than Zhengdan 958. The density is good with density index of 1.12.【 Conclusion】 Xinyu108 has high yield, good stability, good quality, fast dehydration, lodging resistance, resistance to maize smut, head smut and stem rot, and is suitable for mechanical harvesting.
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Deep Sequencing to Identify Maize miRNA Responding to Drought Stress in Floral Organ Differentiation Stages and Its Target Genes
WANG Yejian, LIANG Xiaoling, Abulati Abra, HAN Dengxu, YANG Jie, XI Haojiang, LIU Jun, LI Mingdong
Xinjiang Agricultural Sciences    2020, 57 (8): 1373-1384.   DOI: 10.6048/j.issn.1001-4330.2020.08.001
Abstract123)      PDF (1921KB)(1496)      
【Objective】 Modern molecular biology and high-throughput sequencing technology were applied to screen different miRNAs and their target genes that respond to drought stress during the differentiation of tassel flower organs in different cultivars of corn, thus further excavating and identifying genes related to maize development and reveal signaling pathways and molecular regulatory networks for differences in traits.【Methods】 The drought-tolerant inbred line "PHBA6" and the drought-sensitive inbred line "Ji 63" were used as the research objects. The miRNA library was constructed using deep sequencing technology to identify the differentially expressed miRNAs. The target genes were subjected to functional annotation, cluster analysis and pathway enrichment.【Results】 A total of 337 precursor miRNAs were identified, including 289 known miRNAs and 48 new miRNAs. A total of 155 differentially expressed miRNAs were found in the three libraries in two groups. Target prediction, GO functional classification and genetic and genomic (KEGG) -based functional enrichment showed that these miRNAs might play a role in drought stress by targeting a range of stress-related genes. Analysis showed that at least 55 predicted target genes were further regulated by 60 miRNAs. Further analysis showed that the NAC, MYB and MAPK gene families scored highest under drought stress, indicating that they played an important role in drought resistance of plants. According to the target gene prediction, a series of cotton miRNAs were related to these top genes, including miR164, miR172, miR1520, miR6158, ghr-n24, ghr-n56, and so on.【Conclusion】 miRNAs may play an important role in drought tolerance during the differentiation of maize tassel flower organs. The screening of miRNAs provided new targets for molecular-assisted breeding and transgenic breeding.
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Research on Object Recognition Based on UAV Multispectral Image
WEI Qing, ZHANG Baozhong, WEI Zheng
Xinjiang Agricultural Sciences    2020, 57 (5): 932-939.   DOI: 10.6048/j.issn.1001-4330.2020.05.018
Abstract421)      PDF (1766KB)(1274)      
【Objective】 In view of the lack of timeliness of farmland information acquisition and the difficulty of grasping basic farmland information in time, in this project, the UAV multi-spectral images acquired in May and June 2018 were used to study the extraction of some farmland types in Daxing experimental base in Beijing. 【Method】Firstly, the species of interest were identified, and the temporal and spectral characteristics of the image were analyzed. Then, the normalized vegetation index NDVI, normalized green-blue difference index NGBDI, modified ratio vegetation index MSR and red-band reflectance were determined as the optimal classification features, and the image was segmented by threshold based on spectral variables. The decision tree classification method based on visual interpretation was used to realize the classification of land features and extract the planting area. The method was validated by selecting the ground survey data based on visual interpretation. 【Result】 The results showed that the decision tree classification method based on temporal and spectral characteristics had good effect and the method was applicable to extracting wheat, fruit trees and big shed with errors of 10.68%, 6.06% and 16.48%, respectively. Besides, the area extraction error was within 17%, so we can safely say that UAV multi-spectral remote sensing image has certain applicability for ground object recognition. 【Conclusion】 The advantages of UAV in low cost and high efficiency provide reference for timely access to farmland information.
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Changes of Root Morphology and Its Relationship with Mineral Elements in Different Soil Depths of Cotton (Gossypium hirsutum L.)
DOU Hai-tao, LEI Chang-ying, LI Xia-fei, JIA Meng-meng, XIANG Dao, ZHANG Ya-li, ZHANG Wang-feng
Xinjiang Agricultural Sciences    2019, 56 (8): 1397-1407.   DOI: 10.6048/j.issn.1001-4330.2019.08.004
Abstract436)      PDF (1894KB)(1170)      
Objective】 To study the changes of cotton root morphology and the response to mineral elements in different soil depths and analyze the regulation of mineral elements on root morphology in the hope of providing theoretical support for the construction of reasonable cotton root configuration. 【Method】 The root length, root surface area, root volume, root biomass, root nitrogen, phosphorus, potassium,calcium, magnesium and sulfur of 37 cotton varieties were tested, and according to the formula, the specific root length, specific root area, root volume and root density were calculated. Finally, the regulation and control relationship between mineral elements and root morphology was studied. 【Result】In 0 - 10 cm soil, due to the action of nitrogen, the specific root length, specific root area and specific root volume increased, and the growth of root density was slightly inhibited. In 10-20 cm soil, nitrogen promoted the specific root length, increased the specific root area, and inhibited the growth of root density and the increase of specific root volume. In 20-30 cm soil, the effect of nitrogen on root length, specific root area and root density was not obvious, but the contrastive root volume showed a negative correlation. Nitrogen showed a positive correlation with root length, specific root area, specific root volume, and root density in 30-50 cm soil. In the soil of 50-60 cm, there was a negative correlation between root length, specific root area, and specific root volume and root density. Principal component analysis showed that the correlation between the first principal component and nitrogen element, specific root length, specific root area, and specific volume was higher. The second principal component was more correlated with root density, and the third principal component was mainly related to phosphorus. The correlation between potassium and potassium was high. In addition, the correlation between the phosphorus and potassium elements was higher than that of the first main component, and the correlation between the nitrogen element and the third main component was high. 【Conclusion】 Under different soil depths, the root length, specific root area and specific root volume gradually increased, and the nitrogen content per unit mass root decreased continuously. The root morphological traits were mainly regulated by nitrogen, and the phosphoric and potassium elements had little effect on the regulation of root morphological traits.
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Xinjiang Agricultural Sciences    2024, 61 (8): 0-0.  
Abstract62)      PDF (44862KB)(1150)      
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Study on Fruit Quality and Aroma Components of Main Apple Varieties in Cold Region
LIU Chang, WANG Kun, AN Meng-meng, MEI Chuang, CAO Yang, YU Wen-quan, BU Hai-dong, CHENG Xian-min, GU Guang-jun, MENG Xiang-hai, DONG Xue-mei, CHENG Cun-gang
Xinjiang Agricultural Sciences    2019, 56 (10): 1846-1859.   DOI: 10.6048/j.issn.1001-4330.2019.10.010
Abstract372)      PDF (1112KB)(1106)      
【Objective】 To analyze the main sugar, organic acid and other fruit characters of four main cold resistant apple varieties and study the main aroma components in the hope of providing the basis for the improvement of apple varieties in cold region. 【Method】 Heilongjiang 4 main planting cold-resistant varieties were selected as test materials to analyze the total phenols, antioxidant capacity, flavonoids, pericarp anthocyanins, soluble solids and VC contents of the fruits. Sugar and acid were analyzed by high performance liquid chromatography (HPLC) and the main composition and content of aroma were detected and analyzed by HS-SPME method. 【Result】 Among the fruits of 4 apple varieties, the order of soluble solids content from high to low was the Longguan > Longfeng > Qiyuexian > Jinhong. On the whole, the total sugar contents from high to low were: Jinhong >Longfeng > Qiyuexian > Longguan. From the point of view of total acid content, they were: Jinhong > Qiyuexian >Longfeng > Longguan. A total of 9 types of volatile compounds were detected, of which 7 types played the main roles, and the sequence of components from large to small was: ester > olefin >heterocyclic >acids > alcohol > aldehyde > alkane. Fruit aroma substances mainly concentrated in alcohols, olefins and esters. Among them, the ester content was the highest (56.24%), and the main expression was "fruit aroma" taste. There were significant differences in sugar - acid ratio and taste evaluation among different varieties. The maximum amount of Longguan was 22.96, and the lowest in July Fresh was 9.92, showing a taste of acid. 【Conclusion】 The sugar and acid composition and content of the fruits of the four apple varieties were significantly different, all of which were "ester aroma type " apples, indicating that esters may play a decisive role in the formation of the fruit flavor of these fruits.
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Effects of Uniconazole on the Growth of Branches and Leaves and Fruit Quality of Korla Fragrant Pear
ZHANG Shikui, Abulaike Niyazi, WANG Shaopeng, WANG Yatong, SUN Zhaozhan, FAN Guoquan
Xinjiang Agricultural Sciences    2020, 57 (9): 1674-1680.   DOI: 10.6048/j.issn.1001-4330.2020.09.012
Abstract701)      PDF (1411KB)(1088)      
【Objective】 The purpose of this study is to explore the effects of Uniconazole treatment with different concentrations on the branch growth and fruit quality of Korla Fragrant Pear in the hope of providing theoretical basis for the fruit cultivation.【Method】 On the 25th day after anthesis, Uniconazole with concentration of 50, 150, 250, 350, 450 mg/L was sprayed on the leaves of the new shoots of Korla Xiangli Pear. The growth indexes such as the length and coarseness of the new shoots were measured regularly, and the fruit quality of each treatment was measured after the fruit matured.【Results】 Uniconazole treatment effectively inhibited the increase of shoot length, internode length, leaf area and chlorophyll value of Korla Fragrant Pear. With the increase of treatment concentrations, the inhibition effect was more obvious, and effectively promoted the increase of shoot diameter. The effect of 150-450 mg/L Uniconazole was obvious. After treatment with Uniconazole, the weight of single fruit, soluble solids, VC content and total soluble sugar of Korla Fragrant Pear were increased to some extents; Meanwhile, with the increase of treatment concentrations, the ratio of stone cells to rough fruits was also increased, and the effect of 450 mg/L Uniconazole was the most significant.【Conclusion】 Spraying 250-350 mg/L of Uniconazole on the leaves during the rapid growth period of the new shoots of Korla Fragrant Pear can effectively inhibit the growth of the new shoots, but has no obvious effect on the fruit quality. It can be applied to the regulation of the growth and development of Korla Fragrant Pear.
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Relationship of Muscle Fiber Characteristics with Meat Quality and Its Breed and Sex Differences in Pigs
OU Xiu-qiong, LI Xing, ZHONG Zheng-ze
Xinjiang Agricultural Sciences    2019, 56 (12): 2345-2352.   DOI: 10.6048/j.issn.1001-4330.2019.12.022
Abstract374)      PDF (1047KB)(1010)      
Objective】 The purpose of this article is to understand the relationship of muscle fiber characteristics with meat quality and its breed and sex differences in pigs by collecting and analyzing the relative references. 【Result】①The formation of muscle fibers is a very complex biological process. The number, size and type of muscle fibers are the basic characteristics of muscle fibers. ②At present, the most commonly used classification method of muscle fibers is based on the polymorphic expression of MyHC gene in muscle fibers. ③Before birth, MyHC isomers were expressed in the order of embryonic, fetal and mature stages . After birth, the muscle fiber types were transformed into each other. ④Different types of muscle fibers can influence muscle color, pH, water hold capacity and so on because of their different metabolic characteristics. The size and density of muscle fiber also affect muscle quality because they are closely related to the type of muscle fiber. ⑤Different breeds of pigs show different muscle quality because of their different muscle fiber characteristics. The characteristics of muscle fibers are different between the sexes in order to adapt to their own growth and physiological needs.【Conclusion】The characteristics of muscle fiber in pigs directly affect the meat quality. Because of the various breeds and sexes, the formation of muscle fiber characteristics is different, which shows muscle quality is different.
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Comparative Study on Production Performance of 10 Oat Varieties
WANG Yanchao, SONG Lei, ZHANG Fanfan, WANG Xuzhe, SUN Yanmei, MA Chunhui
Xinjiang Agricultural Sciences    2020, 57 (2): 254-263.   DOI: 10.6048/j.issn.1001-4330.2020.02.006
Abstract528)      PDF (2090KB)(949)      
【Objective】 By observing the agronomic traits, production performance and nutritional quality of 10 oat varieties at different growth stages, the varieties suitable for local cultivation were selected. 【Method】 The following varieties were selected for experiments: Qingyin 1, Denmark 440, Monida, Jiayan 2, Bayan 3, Charisma, Forage plus, Lena, Baler 2, Everleaf. They were sown on April 28, 2018, and the agronomic traits at heading stage, milking stage and maturity stage were observed, and the nutritional indexes of each variety were measured at the same time. 【Result】 The growth period of early-maturing varieties was 78-83 days, the early-maturing and mid-maturing varieties were 88 days, the middle-maturing varieties were 88-92 days and the late-maturing varieties were 91-103 days. The plant height increased with the growth of oats. The plant height of Baylor 2 was the highest and at the maturity period it reached 128.26 cm. The growth rate of Jiayan 2 at heading stage was significantly higher than that of other varieties (P< 0.05). The yield of hay in milky stage was the highest, and Ivo hay was significantly higher than other oat varieties (P< 0.05), and the yield in milky stage was 10.68 t/hm2. In terms of nutritional quality, crude protein, neutral detergent fiber and acid detergent fiber decreased with the growth of oats, while crude fat increased with the growth of oats. The nutrient indexes of crude protein was between 8.75% and 15.88%, and the crude fat was 1.98%-5.25%, the neutral fiber was 41.84%-59.45%, the acid detergent fiber was 20.35%-39.42%. Everleaf had the highest crude protein content, Lena, Forage plus and Baler 2 had the highest crude fat content. Charisma neutral detergent fibers had the highest content, while Everleaf and Baler 2 neutral detergent fibers had the lowest content. Bayan 3 and Monida acid detergent fibers had the higher content than that of Baylor 2 generation. 【Conclusion】 According to the comprehensive evaluation of membership function, the late-maturing cultivars to be recommended were Everleaf and Baler 2, the early and middle-maturing cultivars to be recommended were Jiayan 2, and the best cutting time was at the milky stage.
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Ecological Vulnerability Assessment in Xinjiang Based on VSD Model
LU Haiyan, SUN Guili, LI Lu, LU Hang, BAI Yichun
Xinjiang Agricultural Sciences    2020, 57 (2): 292-302.   DOI: 10.6048/j.issn.1001-4330.2020.02.010
Abstract412)      PDF (3085KB)(945)      
【Objective】 Ecological vulnerability assessment is an important way to understand ecological status of region. Scientific assessment of ecological vulnerability and its changes is important for the formulation of ecological protection measures and regional sustainable development.【Method】 In this project, 14 prefectures and cities in Xinjiang Uygur Autonomous region were taken as the research object, and the evaluation index system was constructed based on VSD (Vulnerability Scoping Diagram) evaluation model. Principal component analysis and comprehensive index method were used to quantitatively evaluate the ecological vulnerability and comprehensive vulnerability of various prefectures and cities in Xinjiang from 2007 to 2017.【Result】 (1) On the whole, Turpan City was in an extremely vulnerable area affected by climatic conditions, most of the southern Xinjiang was in a severe vulnerable area, while the northern Xinjiang was mainly a mild vulnerable area. (2) Exposure index and sensitivity index of Xinjiang changed little from 2007 to 2017,but the adaptability index increased and the comprehensive ecological vulnerability showed a slight downward trend. (3) The ecological vulnerability was higher in the southern Xinjiang than in northern Xinjiang, which might be closely related to the improvement of adaptability.【Conclusion】The key to reduce the vulnerability is to focus on improving the ecological adaptability of the moderate and severe vulnerable areas.
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The community diversity of butterflies in Huocheng County, Xinjiang
GUO Jie, WU Wei, JIANG Ling-ling, HU Hong-ying
Xinjiang Agricultural Sciences    2019, 56 (3): 446-456.   DOI: 10.6048/j.issn.1001-4330.2019.03.007
Abstract491)      PDF (1223KB)(919)      
Objective In order to explore species diversity of butterfly in five different habitats from Huocheng County .Method Butterfly monitoring was conducted with the line transect method from May to August in 2016 and 2017,and the diversity, evenness and dominance of the community on the levels of family, genus and species were studied by the method of α-diversity.Result The number of 1993 individuals were recorded, which belongs to 7 families, 29 genera and 39 species. Pieridae was the dominant family, which accounts for 54.9%; Parnassiidae、Papilionidae and Hesperiidae were regarded as the rare species. The species richness and quantity of the butterflies are different in different seasons, which reached their peaks in June and July. The diversity and similarity of butterfly communities in different habitats were compared, the diversity indices of butterfly in the typica habitats ranked in descending order of mountain forest > mountain steppe > cropland > subalpine meadow > desert. The largest similarity coefficient of the five different habitats was found between the mountain steppe and subalpine meadow (0.458) , the least was between desert and subalpine meadow (0.2) .Conclusion Our results indicated that butterfly diversity was closely related to the habitat type. At the same time, the factors, including the composition of the plant community, the altitude, the degree of human disturbance and the temperature do have a great influence on the diversity of the butterfly community.
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The Succession of Plant Biodiversity in Different Degraded of Bayinbluk Alpine Grasslands
DONG lei;AN Sha-zhou;DONG Yi-qiang;ZHANG Ai-ning;ZHANG Rui-si;XIA Xiao-wei;Kasidaer·Nuerdanbike
  
Abstract177)      PDF (1222KB)(861)      
[Objective] Bayinbuluke alpine grassland was taken the research object to research the degradation succession of vegetation diversity in the hope of providing a theoretical basis for the scientific management of alpine grassland.[Method]By using the method of space to replace the time degradation gradient degradation successional sequence, the field investigation of plant communities during different alpine grassland degradation stages in Bayinbuluk was carried out.The variation of vegetation diversity was studied by using the index of α and β diversity.[Result]The results showed that Simpson index increased significantly by 9.6;-13.7; compared with non-degradation;and with the increasing degraded succession, Margalef index showed a slight increase and a significant decline trend, but the Shannon-Wiener index and Pielou index showed the fluctuation of upward then downward and upward change.In addition, with the worsening of grassland, the resource heterogeneity was decreased in the quadrat, but increased among quadrats, so the β diversity was increased.Cody index showed the tendency of "up-down", this result showed the species substitution rate increased first and then decreased.Bray-Curtis index was higher in light-degradation, mid-degradation and heavy-degradation grassland than non-degradation grassland, and it indicated that there were more common species among different degraded grasslands.[Conclusion]In the alpine grassland, species composition changed at different degradation succession stages, the forage grass with good palatability reduced, poisonous grass increased, resulting in reduction of the quality of grassland.
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Cloning and Sequence Analysis of 5'UTR Intron of GhFAD2-1 Gene from Cotton (Gossypium hirsutum L.)
SUN Liang, WEN Feng, LIU Feng, ZHANG Xin-yu, SUN Jie
Xinjiang Agricultural Sciences    2018, 55 (1): 1-8.   DOI: 10.6048/j.issn.1001-4330.2018.01.001
Abstract307)      PDF (2046KB)(821)      
【Objective】 Cloning and sequence analysis of the 5'UTR intron of the delta 12- oleic acid desaturase gene GhFAD2-1 would lay the foundation for the study of the expression and regulation of GhFAD2-1 . 【Method】 In this study, the 5'UTR sequence of GhFAD2-1 was cloned by 5'RACE technology. And then, 5'UTR intron of GhFAD2-1 was cloned in combination with the cotton genome sequence. Cis elements were also analyzed by PLACE and other bioinformatics software.【Result】 The GhFAD2-1 5'UTR sequence 77 bp was obtained from Gossypium hirsutum using 5'RACE technique. The result showed that the transcription start site is T based on a combination of 5'UTR and genome sequence analysis. GhFAD2-1 5'UTR contained a full-length 1,103 bp intron sequence in the A genome of cotton. The full-length sequence of GhFAD2-1 5'UTR intron was 1,111 bp in D genome. The cleavage sites of 5' UTR intron were AA-GG, CA-GC, respectively. Cis element analysis showed that the intron included some typical function and optical response related elements and elements related to hormone regulation or stress response.【Conclusion】 5'UTR intron sequences of GhFAD2-1 gene were cloned from the A and D genome, respectively. The transcription start site and the cleavage sites of 5' UTR intron were also identified. The results have laid a foundation to further study expression and regulation of GhFAD2-1 at the molecular level.
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Study on HPLC Fingerprint of Hymenoleana nana
HU Jiang-lan, ZHU Jin-fang, Parhat Dolkun
Xinjiang Agricultural Sciences    2019, 56 (2): 308-316.   DOI: 10.6048/j.issn.1001-4330.2019.02.012
Abstract320)      PDF (1522KB)(819)      
【Objective】 To establish the HPLC Fingerprint of Hymenoleana nana and provide a scientific basis for the quality control, the development and utilization of medicinal plant resources.【Method】HPLC was performed using a WondaCract ODS-2 C18 column(4.6 mm×250 mm,5 μm), with of mobile phase acetonitrile: 0.1% formic acid water gradient elution, flow rate 1.0 mL/min, column temperature 30℃, detection wavelength 283 nm. The fingerprints of 10 batches of medicinal materials were determined and the fingerprints were analyzed by cluster analysis, principal component analysis and least squares-discriminant analysis using the Similarity Evaluation System for Chromatographic Fingerprint of TCM and statistical software.【Result】The chromatographic evaluation software calibrated 13 common chromatograms, and the similarity of 7 fingerprints in 10 batches was >0.9. 【Conclusion】 The established method has good precision, stability and repeatability, which lays a foundation for the total quality control of the medicinal materials of Hymenoleana nana.
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An Anatomical Study on Flower Bud Differentiation of Miguang and Baoguang
JIA Nan, YUAN Jun-wei, HAN Bin, LI Min-min, LIU Chang-jiang, YIN Yong-gang, SUN yan, GUO Zi-juan ZHAO Sheng-jian
Xinjiang Agricultural Sciences    2019, 56 (11): 2015-2022.   DOI: 10.6048/j.issn.1001-4330.2019.11.007
Abstract950)      PDF (1301KB)(813)      
Objective】 The study aims to observe the initiation time of flower bud differentiation and the speed of differentiation in different grape cultivars in the hope of providing theoretical foundation for alternate year bearing. 【Method】 The paraffin section method was used to observe the bud structure in the process of flower (cultivars Miguang, Baoguang and Summer Black).【 Result 】 The results showed that the whole process of the morphological differentiation of flower bud could be divided into the undifferentiating stage, the early differentiation of inflorescence stage, the inflorescence anlagen appearance stage, the formation of the main axis of inflorescence primordium stage, and the second branching of inflorescence primordium stage. The peak period of flower bud differentiation of Summer Black , Miguang and Baoguang occurred between the early June and the early August(about 60 days), between the early June and the mid to late June (about 40 days) and between the late May and the mid to late June(about 50 days) respectively.【Conclusion 】 In general, the morphological characteristics were basically the same in each stage in the three cultivars. The process of flower bud differentiation was slower, the duration of each stage was relatively longer and the stages were more overlapped with each other of Summer Black than Miguang and Baoguang.
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Research on the Impact of Scientific Research Personnel's Scientific Literacy on the Input-Output Efficiency of Scientific Research Innovation
REN Hong-song, CHEN Bao-feng, ZHAO Long, Aihemaiti Maimaiti, WU Jiu-yun
Xinjiang Agricultural Sciences    2018, 55 (12): 2331-2342.   DOI: 10.6048/j.issn.1001-4330.2018.12.021
Abstract314)      PDF (1132KB)(799)      
【Objective】 This research aims to study the impact of scientific research personnel's scientific accomplishment on input-output efficiency of scientific research innovation.【Method】By using DEA-TOBIT two-stage model, and a sample of 178 researchers from the Xinjiang Academy of Agricultural Sciences, we work out the efficiency of scientific research and using researchers' scientific accomplishment to do the impact research into the scientific research. The collection, collation, summary and comparative analysis of relevant literature and questionnaire survey were carried out, and DEA-Tobit two-stage model was used to analyze the efficiency of innovation input-output of each sample, and the relevant variables of scientific research accomplishment were used to study the efficiency.【Result】It was concluded that researchers' scientific accomplishment had a positive impact on the efficiency of their innovation output. However, researchers' scientific literacy only affects the pure technical efficiency of scientific research. By further research, we found that the research accomplishment, individual ability and research foundation of scientific research accomplishment had an impact on the pure technical efficiency. 【Conclusion】Our research has provided a path that improving researchers' scientific research accomplishments can reach a target which promotes scientific research output under constraint conditions.
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Effect of Exogenous Silicon Drip Application on Growth and Physiological Characteristics of Winter Wheat under Low Light Stress
ZHANG Yongqiang, FANG Hui, CHEN Chuanxin, NIE Shihui, Sailihan Sai, XU Qijiang, CHEN Xingwu, LEI Junjie
Xinjiang Agricultural Sciences    2023, 60 (2): 336-343.   DOI: 10.6048/j.issn.1001-4330.2023.02.010
Abstract3047)   HTML12)    PDF (1215KB)(66)      

【Objective】 To explore the effect of exogenous silicon drip application on the growth and physiological characteristics of winter wheat under low light stress. 【Methods】 Pot experiment was conducted in southern Xinjiang from 2018 to 2019, and Xindong 60 was used as the experimental material. From jointing stage to mature stage, the winter wheat was shaded by black shading net at 25%. At the jointing stage of winter wheat, five exogenous silicon (Na2SiO3analysis pure) were applied at the drip rates of 7.5 kg/hm2 (Si1), 15 kg/hm2 (Si2), 22.5 kg/hm2 (Si3), 30 kg/hm2 (Si4) and 37.5 kg/hm2(Si5), respectively. 【Results】 The results showed that under low light stress, exogenous silicon drip application could increase the SPAD value of plant height, stem diameter, ear length and leaves of winter wheat The photosynthetic performance of leaves and the net photosynthetic rate (Pn), transpiration rate (Tr) and stomatal conductance (Gs) of flag leaves were improved. The number of fertile spikelets increased and the number of infertile spikelets decreased. The biomass per stem, grain number per spike and grain weight per spike increased significantly. Each index reached the best in Si3 treatment. 【Conclusion】 Comprehensive analysis shows that silicon can improve the growth of winter wheat plants under low light stress, improve photosynthesis, and increase the number of fertile spikelets, grains per spike and grain weight per spike. Under the experimental conditions, the best application of exogenous silicon is 22.5 kg/hm2, which can provide reference for production.

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The Occurrence and Damage of Wheat Powdery Mildew in Desert Oasis Area and the Control Efficiency Evaluation of Fungicides
CHEN Li, SHEN Yuyang, CUI Yanhua, ZHANG Hang, YANG Anpei, FAN Guiqiang, LEI Junjie, LI Guangkuo, GAO Haifeng
Xinjiang Agricultural Sciences    2022, 59 (5): 1189-1195.   DOI: 10.6048/j.issn.1001-4330.2022.05.018
Abstract2872)   HTML5)    PDF (863KB)(39)      

【Objective】 The aim of this study is to clarify the occurrence and damage of wheat powdery mildew in different areas and screen the fungicides which have better control effectsin the hope of providing scientific basis for the prevention and control of wheat powdery mildew in desert oasis ecological area. 【Method】 General investigation method was used to investigate the occurrence of powdery mildew in different ecological zones. The efficacy test was conducted by randomized blockdesign, and the occurrence of wheat powdery mildew was surveyed in each plot at fixed points. 【Result】 The occurrence and damage of wheat powdery mildew in Hotan, Kashgar and Aksu regions were higher, with disease index of (35.65±0.83),(35.63±1.04) and (30.82±0.91), respectively, significantly higher than that in Changji Hui Autonomous Prefecture and Tacheng region, with the disease index of (9.95±0.71) and (6.00± 0.46), respectively. 240 g/L mefentrifluconazole-pyraclostrobine EC and 19% picoxystrobin-propiconazole SC had the best control efficacy against wheat powdery mildew with 84.94% and 82.77%, respectively. The control efficiency of 23% kresoxim-methyl-epoxiconazole SC, 430 g/L tebuconazole SC, 42% metrafenone SC, 43%fluoxastrobin-tebuconazole SC, 48% difenoconazole-azoxystrobin SC, 75% trifloxystrobin-tebuconazole WG and 40% myclobutanil WP floated in the range of 64.57% to 71.12%. 【Conclusion】 The occurrence and damage of wheat powdery mildew in southern Xinjiang is significantly severer than that in northern areas, with the occurrence degree of 3 to 5. The occurrence of powdery milder in northern Xinjiang was mostly at degree 1 to 2. 240 g/L mefentrifluconazole-pyraclostrobine EC, 19% picoxystrobin-propiconazole SC, 23% kresoxim-methyl-epoxiconazole SC, 430 g/L tebuconazole SC, 42% metrafenone SC, 43% fluoxastrobin-tebuconazole SC, 48% difenoconazole-azoxystrobin SC, 75% trifloxystrobin-tebuconazole WG and 40% myclobutanil WP can effectively control wheat powdery mildew and are safe for wheat growth. They can be applied in rotation in wheat fields.

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Effects of different growth regulator and its application times on stem characteristics and lodging resistance of winter wheat under drip irrigation
ZHANG Jingcan, XU Qijiang, ZHANG Yongqiang, LEI Junjie, LYU Xiaoqing, CHEN Chuanxin, NIE Shihui, XU Wenxiu
Xinjiang Agricultural Sciences    2025, 62 (1): 37-44.   DOI: 10.6048/j.issn.1001-4330.2025.01.005
Abstract2867)   HTML7)    PDF (1136KB)(19)      

【Objective】 To select the most suitable growth regulators and the application times of regulators in the hope of providing theoretical basis for lodging resistance and control measures of Xinjiang drip irrigation wheat. 【Methods】 Xinjong 18 were taken as the material, and different regulators as the main zones under the field drip irrigation conditions from 2021 to 2022: clear water control (CK), calcium modulate · Enodozole (A), nanocin (B), enodozole·methylpiperonium (C), antyloxyl ester (D), polylodozole (E), and in addition, the frequency of regulator application was used as the secondary zones: A total of 12 treatments were applied at rise stage (P1) and rise stage + jointing stage (P2) to analyze the effects of different treatments on plant height, center of gravity height, internode length, stem diameter, stem bending resistance and yield of winter wheat under drip irrigation. 【Results】 The results showed that the plant height could be controlled effectively, the center of gravity height and internode length could be reduced, and the bending resistance of the basal stems could be improved. The bending resistance of the basal stems was increased by 8.97% and 14.89% in the treatment of one-time and twice spraying of regulator. BP2 treatment could effectively increase the effective panicle number and 1000-grain weight, and the panicle number increased significantly under CP2 treatment. Under the premise of spraying one regulator, the yield increased by more than 10% under AP1, BP1 and CP1 treatments, and under the premise of spraying two regulators, the yield increased by more than 10% under AP2, BP2, CP2 and DP2 treatments. 【Conclusion】 The plant height of AP2 and BP2 are relatively suitable, the stem lodging resistance is better, and the grain yield is increased greatly.

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Variation of flour color (whiteness) of Xinjiang wheat varieties and its influencing factors
LIU Na, Halidan Yikeremu, LIU Lianzheng, CAO Junmei, ZHOU Anding, ZHANG Xinzhong, Damailijiang Hezier
Xinjiang Agricultural Sciences    2023, 60 (10): 2426-2432.   DOI: 10.6048/j.issn.1001-4330.2023.10.011
Abstract2654)   HTML14)    PDF (685KB)(53)      

【Objective】 Variation of flour color (whiteness) of Xinjiang wheat varieties and its influencing factors,to improve the flour whiteness of Xinjiang wheat varieties and provide theoretical reference for breeding wheat varieties with excellent flour color in Xinjiang wheat breeding job in the future.【Methods】 In this study, Xinjiang wheat cultivars (lines) were used as materials to understand the distribution law of wheat flour whiteness in this region and then 37 winter wheat varieties (lines) of different quality types in Xinjiang were used as materials to preliminarily explore wheat related quality traits.【Results】 The average distribution range of flour whiteness of 71 Xinjiang wheat variety resources was large, and 13 wheat materials had whiteness values higher than 80; The water absorption rate, grain hardness of flour and whiteness were extremely significantly negatively correlated, and grain hardness, water absorption, starch content and elongation jointly determined 63.3% of flour whiteness variation.There was no significant correlation between grain protein content and flour whiteness, but a very significant positive correlation with flour a* value.【Conclusion】 There are few germplasms of medium and high whiteness in Xinjiang wheat cultivars (lines).

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Study on the Performance of Quality-related Characters of Main Spring Wheat Planted in Xinjiang
LI Jianfeng, FAN Zheru, ZHANG Yueqiang, WANG Zhong, GAO Xin, SHI Jia, ZHANG Hongzhi, WANG Lihong, ZHAO Qi
Xinjiang Agricultural Sciences    2021, 58 (12): 2157-2168.   DOI: 10.6048/j.issn.1001-4330.2021.12.001
Abstract2609)   HTML3)    PDF (1647KB)(203)      

【Objective】 To study the performance and stability of quality-related characters of the major varieties of Xinjiang spring wheat in four different ecological regions. 【Methods】 In the four different ecological regions, eight major cultivars of Xinjiang spring wheat with different gluten strengths were taken as research object and based on the quality-related characters of the wheat, ten quality indexes, including grain protein content, were determined with the aid of PertenDA7200 near-infrared analyzer and with the method of AACC39-10 under different ecological conditions. 【Results】 Content of the grain protein14.28%, degree of gluten strength31.21g/100 g, dough stabilization time: 8.41min, water absorption58.53%, sedimentation value 31.27 mL, flour yield 74.40%, maximum tensile resistance Rm 716.25EU, extension E 165.70 mm, stretching area 119.33 cm 2, volume weight793.97 g/L. Among the ten quality characters, flour yield (%), water absorption (%) and volume weight (g/L) of the four varieties in strong gluten strength group were lower than those in middle gluten strength group. The average performance of the other seven quality characters, including content of the grain protein, degree of gluten strength, dough stabilization time, sedimentation value, maximum tensile resistance, extension and stretching area of the four varieties in strong gluten strength group were higher than those in middle gluten strength group. The character and stability of Xinchun No. 26 and Xinchun No. 44 in strong gluten strength group tested and that of Xinchun No. 6 and Xinchun No. 40 in middle gluten strength group tested all had good performance in four different ecological regions. According to the quality-related characters F test, three characters of content of the grain protein, degree of gluten strength and water absorption had the largest genotype effect in strong gluten strength group and the largest environmental effect in middle gluten strength group, and the other seven characters had the largest environmental effect both in strong gluten strength group and middle gluten strengthgroup. 【Conclusion】 Referring to the review and approval standards for national-level wheat variety, overall quality of the eight varieties tested is evaluated as moderately strong gluten strength level. Ten quality characters are all affected by genotype, environment and interaction; Genotype, environment, and interaction effects of different quality characters of different varieties tested with different gluten strength are different. Stability of the same quality character of different varieties tested is different, and that of different quality characters of the same variety tested is also different. Xinchun No. 6 and Xinchun No. 40 in middle gluten strength group have good performance in character and high stability, and they are the core parent material of spring wheat breeding; Xinchun No. 26 and Xinchun No. 44 in strong gluten strength group have good performance in character and high stability, which can be used for quality improvement in Xinjiang spring wheat.

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Regulation of chlormequat on lodging resistance of winter wheat stem during the period of drip application of CCC
ZHANG Yongqiang, CHEN Chuanxin, NIE Shihui, XU Qijiang, Sailihan Sai, LEI Junjie
Xinjiang Agricultural Sciences    2023, 60 (8): 1873-1878.   DOI: 10.6048/j.issn.1001-4330.2023.08.007
Abstract2567)   HTML15)    PDF (698KB)(51)      

【Objective】 To explore the regulation of chlormequat on lodging resistance of winter wheat stalks at different drip application periods, determine the best drip application period of chlormequat, and provide theoretical basis for drip irrigation wheat lodging resistance and its control measures in Xinjiang.【Methods】 In 2019-2020, the potted plant simulation field drip irrigation mode was adopted, and based on the previous research that the best chlormequat drip rate of 4,500 mL/hm2 was obtained, the greening period (S1), the rising period (S2), and the jointing period (S3) and the non-drip chlormequat control (S0), a total of 4 treatments with different chlormequat application periods were set to study the effects of different treatments on the drip irrigation wheat plant height, stem thickness, center of gravity height, the characteristics of the second internode at the base, and lodging resistance index and the impact of grain yield.【Results】 The results showed that the drop application of chlormequat during the rising stage had a more significant effect on the reduction of the height of the wheat plant height and the height of the center of gravity than the greening and jointing stages.3.38 mm, the maximum wall thickness was 0.543 mm, the maximum internode fullness was 25.57 mg/cm, the maximum bending resistance was 5.44 N, and the stalk lodging resistance index was the maximum 15.96 N/m.Compared with S0 treatment, chlormequat drip application increased the single-stem biomass of wheat, and the maximum single-stem biomass of wheat treated with S2 was 3.42 g/stem.The most fertile spikelets were treated with S1, the most sterile spikelets were S0, and the number of grains per spike was 38.50 grains at most in the S1 treatment.The grain weight per spike was 1.68 g in the S2 treatment, which was not significantly different from those of the S1 and S3 treatments.The difference between treatments reached a significant level (P<0.05).【Conclusion】 Comprehensive analysis showed that dripping chlormequat during the greening stage, rising stage and jointing stage can reduce the plant height of drip-irrigated wheat and improve its lodging resistance, but the dripping application at the rising stage has the best effect.

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Studies on Expression of Hypoxia Inducible Factor, Erythropoietin Gene and Its Receptor in Ovine Tissues
LEI Yan, LAN Bin, YU Wanli, DAI Xiaohua, CAI Peng, GU Weifang, Adilai Ali, ZHAO Hongqiong
Xinjiang Agricultural Sciences    2023, 60 (2): 485-492.   DOI: 10.6048/j.issn.1001-4330.2023.02.028
Abstract2547)   HTML7)    PDF (1292KB)(48)      

【Objective】 To investigate the expression of hypoxia inducible factor (HIF) and its downstream target gene erythropoietin (EPO) and its receptor (EPOR) in ovine tissues under normal physiological conditions.The results have provided a histological basis for the study of targeted regulation or alleviation of ovine hypoxia stress. 【Methods】 The relative expression of HIF, EPO and EPOR genes in 14 tissues of 4 Xinjiang fine wool sheep were detected by real-time fluorescence quantitative PCR. 【Results】 HIF-1α and HIF-2α the relative expression of genes in ovine lung tissues were the highest and significantly higher than those in other tissues (P< 0.01); HIF-2α in lung the gene expression was about HIF-1α 5.2 times.The relative expression of EPO gene in ovine kidney was the highest and significantly higher than that in pituitary, colon, spleen, cecum, liver, adrenal gland, rumen, hypothalamus and heart (P< 0.05).The expression of EPOR gene in lung was the highest, and the relative expression of lung and testis was significantly higher than that in spleen, hypothalamus, kidney and heart (P< 0.05). 【Conclusion】 HIF-1α, HIF-2α and EPO and its receptor genes are highly expressed in the lung or kidney of sheep, suggesting that these two may be important organs for ovine hypoxia stress perception and regulation.

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Effects of different ecological conditions on dry matter accumulation and yield of spring wheat varieties
DONG Yanxue, JIA Yonghong, ZHANG Jinshan, LI Dandan, WANG Kai, LUO Siwei, WANG Runqi, SHI Shubing
Xinjiang Agricultural Sciences    2023, 60 (8): 1848-1857.   DOI: 10.6048/j.issn.1001-4330.2023.08.004
Abstract2497)   HTML21)    PDF (1232KB)(57)      

【Objective】 To study the effects of different ecological conditions in northern Xinjiang on dry matter accumulation and yield formation of spring wheat, and provide a reference for formulating breeding goals and achieving high yield and high quality of spring wheat.【Methods】 A two-factor experimental design was adopted, 5 varieties: Xinchun 44, Xinchun 37, Xinchun 48, Xinchun 26 and Ningchun 16 were planted in three ecological conditions of Qitai area, Mulei area and Tacheng area, to study the effects of different ecological conditions on leaf area index, dry matter accumulation and distribution, yield components and yield of spring wheat.【Results】 The leaf area index, dry matter accumulation in vegetative growth stage and reproductive growth stage, grain filling capacity and yield of spring wheat were the largest in Qitai.The dry matter accumulation in vegetative growth stage, reproductive growth stage, grain filling sink capacity and yield of spring wheat varieties in Qitai were 26.73%, 32.20%, 11.66% and 45.87% higher than those in Mulei, 21.33%, 17.29%, 1.17% and 17.58% higher than those in Tacheng.The grouting rate was the smallest at Qitai, the second in Techeng, and the largest in Mulei.Under the ecological conditions of Qitai, the yield of Xinchun37 was 0.92%~8.25% higher than those of other varieties.Under Mulei ecological conditions, the yield of Xinchun37 was 6.14%~19.52% higher than those of other varieties.Under Tacheng ecological conditions, the yield of Xinchun44 increased by 4.12%~19.64% compared with other varieties.【Conclusion】 Variety characteristics and yield, spring wheat is the most suitable to be planted in Qitai ecological area, namely, Xinchun 37 should be planted in Qitai and Mulei ecological areas, and Xinchun 44 is appropriate to be planted in Tacheng ecological areas.

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Effects of biomass charcoal application rate on the growth, development, and yield of winter wheat under drip irrigation
CHEN Chuanxin, ZHNAG Yongqiang, NIE Shihui, KONG Depeng, Sailihan Sai, XU Qijiang, LEI Junjie
Xinjiang Agricultural Sciences    2023, 60 (9): 2146-2151.   DOI: 10.6048/j.issn.1001-4330.2023.09.008
Abstract2477)   HTML21)    PDF (861KB)(65)      

【Objective】 In order to study the effect of biomass charcoal application rate on the growth, development, and yield of winter wheat under drip irrigation. 【Methods】 Under drip irrigation conditions, a single factor randomized block experimental design was used to set up three levels of biochar application, namely 0 t/hm2 (CK), 20 t/hm2 (T1), and 40 t/hm2 (T2), to study the differences in growth, development, and yield of winter wheat under drip irrigation under different treatments. 【Results】 The application of biochar improved the leaf area index of winter wheat, and the leaf area index of T2 and T1 treatments was significantly higher than that of CK and increased the chlorophyll activity of winter wheat leaves. The SPAD values of chlorophyll in T2 treatment increased by 0.55%, 0.31%, 0.90%, 2.28%, and 1.41%, 1.18%, 2.95%, and 3.67%, respectively, compared to T1 and CK treatment at the jointing, heading, flowering, and filling stages. In addition, the application of biochar significantly increased the dry matter accumulation of winter wheat and promoted the formation of winter wheat yield, with T2 treatment performing the best at 8,244.40 kg/hm2. T2 treatment increased yield by 5.25% and 12.58% compared to T1 and CK treatments, respectively. 【Conclusion】 Under the conditions of this experiment, it can be found that the application of biochar is beneficial for the growth and yield of wheat. Under the T2 treatment of 40 t/hm2 of biochar application, winter wheat yield performs well, which provides reference for field production.

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Morphological and ISSR Analysis of Purple Sweet Potato Resources
WU Qiaoyu, HE Tianjiu
Xinjiang Agricultural Sciences    2022, 59 (7): 1625-1631.   DOI: 10.6048/j.issn.1001-4330.2022.07.008
Abstract2392)   HTML32)    PDF (697KB)(65)      

【Objective】 To analyze the genetic diversity of purple sweet potato varieties collected from China, clarify their genetic differences in the hope of providing a theoretical basis for the selection of purple sweet potato genetic breeding parents. 【Methods】 Morphological characters and ISSR fluorescent labeling capillary electrophoresis were carried out to analyze the genetic diversity of varieties of 10 purple sweet potatoes imported from other provinces. 【Results】 The results showed that, based on the cluster map of 20 morphological traits, the tested materials could be divided into two major groups at the Euclidean distance of 9.97. The first major category included Ningzi-1, Shandongzi, Yuzi-2, Xuzi-8, Yuenanzi; the second major category consisted of E-12, Ningzi-4, Yuzi-3, Yuzi-11, Nanzi-018. 6 pairs of primers were used in ISSR molecular marker analysis, the results showed that, the observed alleles (Na) of the purple sweet potato germplasm resources were 2, the average effective allele number (Ne) was 1.9190, and the expected heterozygosity (He) was 0.2375 and Shannon's index (I) was 0.4333. Cluster analysis performed with NTSYSpc 2.1 software demonstrated that when the genetic distance was 0.96, the 10 tested purple sweet potato materials could be divided into two categories. 【Conclusion】 The clustering results of SSR markers and morphological markers of several tested materials were consistent in genetic background and group classification, but the cluster analysis results of the two identification and classification methods also had certain differences. The purple sweet potato resources presented very rich genetic diversity. The combination of morphology and ISSR markers could effectively improve the identification of specific varieties and determine the genetic relationship of germplasm resources objectively and accurately.

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Analysis of character variation and heterosis of F1 generation of different crosses in wheat
FENG Mei, LIU Chaoqin, CHEN Jie, LIU Wenlong, YANG Zhigang, TIAN Jieying, HUANG Xin
Xinjiang Agricultural Sciences    2023, 60 (8): 1858-1865.   DOI: 10.6048/j.issn.1001-4330.2023.08.005
Abstract2322)   HTML7)    PDF (703KB)(56)      

【Objective】 To explore the rules of genetic variation and heterosis of F1 characters in wheat hybrids.【Methods】 The cross combination with Han 5316 was taken as female parent and six different wheat varieties as male parent and their female parent were used as materials in this study, variance analysis, correlation analysis and principal component analysis were used to analyze 7 phenotypic traits.【Results】 ①The average coefficient of variation of 7 traits ranged from 4.00%-17.71%, indicating that there were abundant genetic diversity in F1 hybrid progeny of wheat hybrids.②The heritability of F1 generation in wheat was different.The heritability of spike length, grains per spike, spike grain weight and 1000-grain weight was the highest, which was more than 100%; ③ The percentage of heterosis was -32.95%-99.13%, and the heterosis of different combinations was different; ④ The 7 characters all affected each other, and the spike grain weight, grains per spike and 1000-grain weight had a greater influence.【Conclusion】 Two excellent hybrid combinations 18-4 and 18-5 were selected by principal component analysis, and their progenies were characterized by long spike, more grains per spike and larger grains per spike.

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Analysis of dry matter production, transport and nitrogen fertilizer utilization caused by yield Gap at different yield levels of winter wheat
WANG Lihong, ZHANG Hongzhi, ZHANG Yueqiang, LI Jianfeng, WANG Zhong, GAO Xin, SHI Jia, WANG Chunsheng, XIA Jianqiang, FAN Zheru
Xinjiang Agricultural Sciences    2023, 60 (9): 2152-2162.   DOI: 10.6048/j.issn.1001-4330.2023.09.009
Abstract2302)   HTML6)    PDF (1072KB)(53)      

【Objective】 To reveal the differences in the accumulation, transport and utilization of dry matter and nitrogen in winter wheat at different yield levels, and to provide a theoretical basis for reducing the yield difference and high-yielding cultivation of winter wheat in Xinjiang.【Methods】 The experiment was carried out in military households in Changji from 2018 to 2020 with Xindong 41 as the test material, and referring to the production practice in Xinjiang, different fertilization and cultivation management measures were used to simulate high yield I (SH: ≥9,000 kg/hm2), high-yield Ⅱ (HH: 7,500 kg/hm2~9,000 kg/hm2), farmers (FP: 6,000 kg/hm2~7,500 kg/hm2), basic (CK: ≤4,500 kg/hm2) four yield levels.Meanwhile, differences in nitrogen accumulation and transport, and nitrogen absorption and utilization were studied.【Results】 The results showed that the two-year average yield difference of SH, HH, FP and CK was 6,863.27 kg/hm2, 5,496.76 kg/hm2, 3,735.73kg/hm2.The number of harvested panicles and grains per panicle were as follows: SH>HH>FP>CK, and the 1000-grain weight was as follows: HH>SH>FP>CK.The difference of dry matter accumulation in flowering stage of SH, HH, FP and CK is 11,221.65 kg/hm2, 8,220.05 kg/hm2, 5,527.81 kg/hm2, and the difference in dry matter accumulation in mature stage was 16,026.10 kg/hm2, 11,918.25 kg/hm2, 7,645.80 kg/hm2, with the increase of the yield gap, the dry matter accumulation, pre-flowering dry matter transport, post-flowering dry matter accumulation and contribution to grains increased, and pre-flowering dry matter transport and contribution to grains increased; nitrogen accumulation in vegetative organs and pre-flowering nitrogen transport in flowering and mature stages increased with the increase of yield level.Efficiency and nitrogen partial productivity were lower; correlation analysis showed that grain yield was significantly positively correlated with pre-flowering dry matter transport, post-flowering dry matter accumulation, pre-flowering nitrogen transport, nitrogen demand and nitrogen absorption efficiency was significantly positively correlated with nitrogen partial productivity.【Conclusion】 To reduce the yield difference, cultivation and management measures such as fertilization should be adopted, especially the drip irrigation of phosphorus and potassium fertilizers in the late growth stage with water to increase the accumulation and transport rate of dry matter and nitrogen before flowering, the accumulation of dry matter after flowering, and increase nitrogen.In the technology of suitable harvesting panicle number, synergistically increases the number of grains per panicle and 1000-grain weight.

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Effects of chasing rate during peak nutrient uptake of transport under n Reduction on spring wheat
LI Huaisheng, AI Hongyu, MENG Ling, WANG Heya, ZHANG Lei, AI Haifeng
Xinjiang Agricultural Sciences    2023, 60 (8): 1866-1872.   DOI: 10.6048/j.issn.1001-4330.2023.08.006
Abstract2290)   HTML11)    PDF (703KB)(49)      

【Objective】 To provide a basis for reasonable nitrogen reduction in high-yield and high-quality cultivation of spring wheat under drip irrigation in Northern Xinjiang and to determine the amount of nitrogen fertilizer from nodulation to flowering.【Methods】 Xinchun 43 was taken as the test material, on the basis of investigating the nitrogen application amount of 390 kg / hm2 in 213 agricultural households, three nitrogen application levels (312, 270 and 234 kg / hm2) and the operation research ratio from nodulation to flowering (7∶3, 5∶5 and 6∶4) were set according to the nitrogen reduction of 20%, 30% and 40%, to study the effect of the transport ratios from nodulation to flowering on drip irrigated spring wheat.【Results】 Under the condition of N1, the yield of R3 was the highest, which was increased by 7.4% and 3.7% compared with those of R1 and R2.The dry matter accumulation increased by 13.9%, 17.5%, 26.2% and 10.3% compared with that of R1, and 7.7%, 3.6%, 10.6% and 2.6% compared with R2 in each period, respectively; There was no significant difference in assimilation transport before and after anthesis, and the leaf area index was R3 > R2 > R1.Under N2 condition, the yield was consistent with that under N1 condition.The dry matter accumulation was R2 > R1 > R3 in each period.The assimilation transport before and after flowering showed a trend of "increasing first and then decreasing", which reached the maximum in R2, 2,614.35 and 6,284.79 kg / hm2 respectively.The leaf area index was R3 > R2 > R1.Under the condition of N3, the yield of R2 was significantly higher than those of R1 and R3, with an increase of 25.4% and 32.0%, respectively.The dry matter accumulation increased by 4.8%, 10.8%, 8.5% and 23.2% compared with that of R1.The assimilates content before and after anthesis of R3 was maximum at 2,242.32 kg/hm2, which increased by 50.6% and 36.8% compared with R1 and R2, respectively, and the leaf area index showed R2>R1>R3.【Conclusion】 The pure nitrogen 234 kg/hm2 and the N fertilizer transportation ratio of 6:4 from nodulation to flowering are more suitable for spring wheat production under the conditions of this experiment.

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Responses of photosynthetic characteristics, dry matter accumulation and yield to drought stress in winter wheat at jointing stage
WANG Xiaoyu, WANG Xiaoping, SHI Wenyu, LIU Meiyan, MA Jian, GUO Yunpeng, SONG Ruixin, WANG Qingtao
Xinjiang Agricultural Sciences    2023, 60 (9): 2163-2172.   DOI: 10.6048/j.issn.1001-4330.2023.09.010
Abstract2244)   HTML5)    PDF (1479KB)(72)      

【Objective】 To study the photosynthetic characteristics, dry matter accumulation, yield to drought stress in the hope of providing a theoretical basis for spring irrigation schedule of winter wheat.【Methods】 A pot culture experiment was conducted with four soil moisture gradients containing full irrigation (CK), mild drought(T1), moderate drought(T2) and severe drought(T3) in winter wheat at jointing stage in the study.【Results】 The results showed that highest leaf water use efficiency of winter wheat at jointing stage was observed in T2.The trend of net photosynthetic rate, transpiration rate, stomata conductance, intercellular carbon dioxide concentration and chlorophyll content decreased with increasing of drought stress.The rank of the effect of drought stress on fluorescence parameters including Fv/Fm, Fv/Fo and Fm/Fo was that: T1 > CK > T2 > T3.Compared with CK, dry matter accumulation of underground increased by 11.8% and 3.0% in T1 and T2, however, which decreased by 4.0% in T3.The proportion of underground dry matter in T1, T2 and T3 treatment increased by 4.0%, 6.0% and 11.0%, respectively.With respect to the performance of irrigation water use efficiency, it was the highest in T1 and the grain yield increased slightly.In terms of grain yield, it was significantly lower than CK by 14.8% and 42.4% (P<0.05)both in T2 and T3, in turn, which increased somewhat in T1.According to the results of Pearson correlation analysis, leaf water use efficiency was significantly (P< 0.05) positively correlated with total dry matter accumulation, grain yield and irrigation water use efficiency, furthermore, a significant(P< 0.01) positive correlation was detected between leaf water use efficiency and dry matter accumulation of aboveground, but a significant(P< 0.01) negative correlation was found between leaf water use efficiency and intercellular carbon dioxide concentration.【Conclusion】 Under the mild drought treatment, the jointing winter wheat is adapted to the drought environment by regulating photosynthesis and increasing the underground accumulation of some dry matter and its proportion, thus improving the grain yield and irrigation water utilization efficiency.However, with the aggravation of drought stress, the photosynthetic rate of winter wheat decrease obviously, which lead to reducing dry matter accumulation and ultimately lead to the reduction of winter wheat yield.

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Differences of Grain Yield, Nitrogen Uptake and Utilization Efficiency of Different Wheat Varieties in Southern Xinjiang
ZHANG Hongzhi, GAO Yonghong, WANG Lihong, KONG Depeng, ZHANG Yueqiang, LI Jianfeng, WANG Zhong, GAO Xin, SHI Jia, ZHAO Qi, FAN Zheru
Xinjiang Agricultural Sciences    2022, 59 (8): 1847-1854.   DOI: 10.6048/j.issn.1001-4330.2022.08.004
Abstract2233)   HTML28)    PDF (10549KB)(99)      

【Objective】 To study the difference of yield, N uptake and utilization efficiency and response to N fertilizer among different winter wheat varieties in southern Xinjiang in the hope of providing reference for N efficient breeding, selection of N efficient varieties and optimum application of N fertilizer.【Method】12 varieties(strain) were planted in Southern Xinjiang with 3 N application rates, the yield, nitrogen accumulation, nitrogen use efficiency(NUE), nitrogen uptake efficiency(UPE) and nitrogen utilization efficiency(UTE) of different wheat varieties under different nitrogen fertilizer levels were studied.【Result】With the increase of nitrogen application amount, the number of panicle and grain per panicle of different cultivars increased, the 1000-grain weight decreased, and the nitrogen accumulation and yield increased. The NUE, UPE and UTE decreased with the increase of N application. Cluster analysis was carried out on the N use efficiency of various varieties at different N levels. The varieties with higher NUE were Xindong 40, Xindong 60, 15/6317. Different nitrogen efficient varieties had different approaches to achieve high NUE. Under the condition of no nitrogen application(N0), Xindong 40, Xindong 60 and 15/6317 had high NUE, which was mainly due to the joint effects of UPE and UTE. The high nitrogen absorption efficiency of new winter 60 and 40 under nitrogen application was due to their higher UPE, while 15 / 6317 high nitrogen utilization rate was the common effect of nitrogen absorption efficiency and nitrogen utilization efficiency.【Conclusion】Different varieties have different approaches to achieve high nitrogen efficiency, so it is necessary to regulate and control the nitrogen absorption and utilization characteristics of different wheat varieties, improve the nitrogen absorption and utilization efficiency of wheat, maximize the exploitation and utilization of their high yield and high efficiency potential, thus achieving the increase in yield and efficiency.

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Comparative Analysis of Gene Expression between Giza 75 and SG 747 Using Cotton Oligonucleotide Microarrays during Fiber Development
SONG Jikun, XIN Yue, LI Longyun, LIU Guoyuan, PEI Wenfeng, MA Jianjiang, QU Yanying, YU Jiwen, WU Man
Xinjiang Agricultural Sciences    2022, 59 (6): 1312-1330.   DOI: 10.6048/j.issn.1001-4330.2022.06.002
Abstract2187)   HTML31)    PDF (1081KB)(67)      

【Objective】 High-throughput gene chip analysis is performed using upland cotton with wide adaptability and high yield and sea island cotton with excellent fiber quality, aiming to screen and identify key genes involved in cotton fiber development. 【Methods】 The upland cotton SG747 (Gossypium hirsutum L.) and Egyptian cotton Giza75 (G. barbadense L.) were used as experimental materials. The Affymetrix Cotton Gene Chip was then used to perform a transcriptome analysis in developing fibers (10 Days after anthesis, DPA). 【Results】 The 3,905 transcripts obtained showed 2-fold or higher levels of expression changes between the two materials. Clustering analysis of these DE genes by Clusters of Orthologous Groups database was performed, which revealed that many genes were related to general function prediction (17.80%), translation, ribosomal structure and biogenesis (16.82%), posttranslational modification (14.32%). According to qRT-PCR analysis, it was speculated that Gra.2198.1.A1_at positively regulated cotton fiber development and Gra.85.1.S1_at, Ghi.249.1.A1_at, Ghi.8448.1.S1_x_at negatively regulated cotton fiber development, and the above genes might be involved in the development of cotton fiber. 【Conclusion】 We screened and identified four key candidate genes related to fiber development by using upland cotton and sea island cotton gene chips combined with qRT-PCR.

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LC/MS-Based Metabolomics Approach for Exploring Plasma Metabolome Modififications before and after 3,600 m Speed Race in Yili horse
ZHANG Shiqi, LI Xiaobin, ZHANG Wenjie, HAN Ming, WANG Shichang, ZHENG Wenxiang, OUYANG Wen, QI Juzhong, YANG Kailun
Xinjiang Agricultural Sciences    2023, 60 (2): 501-510.   DOI: 10.6048/j.issn.1001-4330.2023.02.030
Abstract2155)   HTML9)    PDF (1300KB)(61)      

【Objective】 The aim of this study is to investigate the difference and change of plasma metabolites before and after 3,600 m speed race in Yili horses. 【Method】 According to the genetic pedigree provided by the equestrian club, 5(3♂2♀) unrelated, weight (384.40±34.18)kg, and age 2 speed raced-horses were selected.The horses were trained daily with equal intensity. On the 25 th day of the experimental stage, the 3,600 m speed race was performed 4 h after morning feeding. The horse completed the race at (11.75±0.87) m/s at average speed. The rate of heartbeat and the breathing were measured immediately before and after the race. At the same time, blood was collected, plasma samples were prepared, plasma mass spectrometry was collected, and metabolites were determined by non-target metabolomics method. 【Results】 Compared with the pre-competition heart rate (39.80±0.40) bpm and respiratory rate (14.00±1.80) bpm, the horse heart rate (80.80±7.70) bpm and respiratory rate (78.80±3.90) bpm were significantly increased (P<0.05). Compared with Yili horses plasma at pre-race, the citric acid cycle, metabolism of alanine, aspartic acid and glutamic acid, transformation between pentaccharide and glucuronic acid, metabolism of cystine and methionine, oxidative phosphorylation, fatty acid degradation and sheath lipid metabolism showed significant changes. On the basis of the LC/MS analysis of plasma metabolomics of Yili horse before and after the 3,600 m speed race, LC/MS metabolic profiles of plasma samples of Yili horse 3,600 m before and after the race were different. The differential metabolites involved in citric acid cycle, glycolysis system, fat metabolism, amino acid metabolism. 【Conclusion】 During the 3,600 m speed race, the Yili horse body presents anaerobic, aerobic and mixed oxygen metabolism characteristics show anaerobic, aerobic and mixed oxygen metabolism.

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Influence of sowing dates on cotton seedling aphid population dynamics and cotton yield in the yellow river basin
LI Yaofa, AN Jingjie, DOU Ya'nan, SUN Xiaonuo, ZHANG Qian, LIU Chunhong, DANG Zhihong, GAO Zhanlin
Xinjiang Agricultural Sciences    2023, 60 (10): 2496-2500.   DOI: 10.6048/j.issn.1001-4330.2023.10.019
Abstract2126)   HTML2)    PDF (673KB)(49)      

【Objective】 The population of cotton aphid migrating from overwintering host to cotton field and the environmental conditions in the Yellow River Basin are important factors for the damage caused by this aphid. The paper aims to clarify the effect of sowing date on the aphid population dynamics and cotton yield.【Methods】 Effects of the sowing date April 16, April 26 and May 6 on the aphid population dynamics and cotton yield parameters were studied in cotton field of southern Hebei Province. 【Results】 The results showed that the cotton emergence rate increased with the delay of sowing period, and the cotton emergence rates on April 26 and May 6 were significantly higher than that on April 16. There was an obvious peak of cotton aphids in spring occurrence and a rapid decrease of aphid quantity in the treatments of April 16 and April 26, while there was no obvious peak of Aphids in spring in the treatments of May 6. In terms of yield, cotton yield increased significantly from 3.94 kg (April 16) to 5.46 kg (May 6) with the delay of sowing period. 【Conclusion】 Postponing the sowing dates of cotton can play the role of protecting seedling, reducing early aphid damage and ensuring yield.

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Cloning and Sequence Analysis of cDNA of C3d Gene of Bactrian Camel from Xinjiang
Nasibai Abuduwahapu, GAO Xiaojuan, Kunduziayi Abudushalamu, LI Jiangwei
Xinjiang Agricultural Sciences    2023, 60 (2): 493-500.   DOI: 10.6048/j.issn.1001-4330.2023.02.029
Abstract2115)   HTML7)    PDF (1557KB)(42)      

【Objective】 The C3d gene of camel complement was cloned and its sequence characteristics were analyzed to provide a basis for the study of its adjuvant effect. 【Methods】 Total RNA was extracted from camel liver using Trizol reagent, and cDNA was obtained by reverse transcription.C3d pecific primers were designed to amplify the C3d sequence by PCR technology, and the vector pMD18-T was constructed.The recombinant plasmid was transferred into competent strain and identified by double enzyme digestion.Clustw, DNA Star and Swiss-model software were used to compare the homology of the constructed recombinant C3d gene sequences, establish the phylogenetic tree, and predict and analyze its secondary and tertiary structures. 【Results】 Specific primers were designed and the 909 bp camel complement C3d gene was obtained by PCR amplification.T vector was constructed and transferred into Escherichia coli and a positive clone was obtained.Clustw was used to compare the DNA sequencing results with the sequences of bovine, pig, rabbit and human.The results showed that the cloned Bactrian camel C3d sequence had more than 97% homology with the C3d gene of Camelidae, and the homology with the C3d gene of bovine, pig, rabbit and human was gradually remote (88%, 88%, 83% and 83%, respectively).The sequence of C3d protein was analyzed by Swiss-model software, and the secondary structure of C3d protein was mainly composed of α-helix and β-fold, and it had good immunogenicity and protein-binding activity. 【Conclusion】 The C3d gene of camel complement was cloned.Sequence analysis showed that the C3d gene was relatively conserved in evolution, which provided the experimental basis and technical reference for the adjuvant effect of the gene and the subsequent research.

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EMS mutants of wheat hengguan 35 and identification of the mutants resistant to fusarium crown rot
QIN Pengliang, ZHOU Xiao, Kahsay Tadesse Mawcha, WANG Shuang, LI Jiaqi, LIU Ying, ZHANG Na, YANG Wenxiang
Xinjiang Agricultural Sciences    2023, 60 (9): 2231-2238.   DOI: 10.6048/j.issn.1001-4330.2023.09.018
Abstract2095)   HTML5)    PDF (1079KB)(71)      

【Objective】 Study EMS mutants of wheat hengguan 35 and identification of the mutants resistant to fusarium crown rot.【Methods】 In this study, different concentrations of ethyl methanesul fonate (EMS) were employed on wheat seeds of cultivar Hengguan 35.The untreated Hengguan 35 was used as the susceptible control, and Shiyou 17 was used as the moderately resistant control.Through artificial inoculation of Fusarium pseudograminearum, the resistance of the mutagenized material was identified, and the M3 generation material resistant to FCR was obtained by adding generation in the greenhouse.【Results】 The EMS concentration suitable for Hengguan 35 mutation was finally determined to be 0.4%, and seven mutants showing immunity at the plant stage were obtained from 106 M3 Hengguan 35 materials.The overall disease index of the mutant materials at seedling and adult stages was 34.93% and 34.62% lower than those of the susceptible control, respectively.【Conclusion】 After EMS mutation of wheat Hengguan 35, an appropriate mutation concentration of 0.4% was determined. A total of 7 immune resistant wheat FCR were screened from the M3 generation of the tested mutant varieties.

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