Cloning and functional analysis of LiMYB60 gene from lavender

doi:10.6048/j.issn.1001-4330.2025.08.001

Abstract

Abstract: 【Objective】MYB is one of the important transcription factors involved in the regulation of terpenoid biosynthesis. The function of lavender MYB transcription factor in the accumulation of flower-scented terpenoids is not clear, so the aim of this study is to analyze the biological function of LiMYB60 in the linalool synthesis pathway of lavender and explore its regulatory role in the accumulation of flower terpenoids.【Methods】The expression of LiMYB60 in different tissues of lavender and the expression pattern of LiMYB60 in corolla at different developmental stages were analyzed by real-time quantitative PCR and the function of LiMYB60 gene was studied by subcellular localization, yeast self-activation and Arabidopsis genetic transformation.【Results】TThe open reading frame of LiMYB60 was 945 bp in length, encoding 314 amino acids, with a molecular weight of 35 307.73 Da and an isoelectric point of 7.13. LiMYB60 was a hydrophilic protein without transmembrane structure and signal peptide. The expression level of LiMYB60 gene was the highest in calyx and Corolla, and it was a nuclear localization protein with transcriptional self-activation activity, the expression levels of MEP pathway-related rate-limiting enzymes and the contents of β-cyclocitral, Borneenal and other monoterpenes were higher than those of wild-type Arabidopsis thaliana.【Conclusion】The lavender LiMYB60 gene has the characteristic of MYB transcription factor, which can regulate the synthesis of monoterpenes.

Key words: lavender; LiMYB60; gene cloning; expression analysis; terpenoid accumulation

摘要： 【目的】MYB是参与调控萜类次生代谢物合成途径的重要转录因子之一，薰衣草MYB转录因子在花香萜类物质积累中的功能尚不清晰，研究旨在分析转录因子LiMYB60在薰衣草芳樟醇合成途径中的生物学功能，探究其在花香萜类物质积累中的调控作用。【方法】以杂花薰衣草为材料，克隆LiMYB60基因，利用实时荧光定量PCR分析LiMYB60在薰衣草不同组织的表达以及在不同发育时期花冠的表达模式。通过亚细胞定位、酵母自激活、拟南芥遗传转化等研究LiMYB60基因的功能。【结果】LiMYB60基因开放阅读框（ORF）长为945 bp，编码314个氨基酸，编码蛋白质分子质量为35 307.73 Da，等电点数为7.13，为无跨膜结构、无信号肽的亲水性蛋白；LiMYB60 在花萼与盛开期花冠中的表达量最高；是具有转录自激活活性的核定位蛋白；在LiMYB60转基因拟南芥叶片中，MEP途径相关合成限速酶的表达量与β-环柠檬醛、龙脑烯醛等单萜类物质含量均高于野生型拟南芥。【结论】薰衣草LiMYB60基因具有典型MYB转录因子的特征，对薰衣草中单萜类花香物质的合成具有正向调控作用。

关键词: 薰衣草, LiMYB60, 基因克隆, 表达分析, 萜类物质积累

CLC Number:

MA Yunting, WANG Aifan, YAN Zhongjian, LIAO Yan, Maierziyaguli Abulaikemu, GUO Danli, SU Xiujuan. Cloning and functional analysis of LiMYB60 gene from lavender[J]. Xinjiang Agricultural Sciences, 2025, 62(8): 1821-1832.

马芸婷, 王爱凡, 严中建, 廖燕, 买尔孜亚古丽·阿布来克木, 郭丹丽, 苏秀娟. 薰衣草LiMYB60基因的克隆及功能分析[J]. 新疆农业科学, 2025, 62(8): 1821-1832.

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