Changes in bacterial diversity and community structure in rhizosphere soil of lavender with different continuous cropping years

doi:10.6048/j.issn.1001-4330.2025.03.020

Abstract

Abstract:

【Objective】To explore the structure and diversity characteristics of bacterial communities in the rhizosphere soil of lavender with different continuous cropping years, as well as their evolutionary patterns.This article provides reference for the scientific management and growth and yield increase of lavender soil.【Methods】Three sets of soil samples were collected from Huocheng County, Ili Kazakh Autonomous Prefecture, with different crop continuously years and without lavender planted. The 16S rDNA sequence was subjected to high-throughput Illumina sequencing. Afterwards, the sequencing results were analyzed, the bacterial diversity and community distribution patterns of each sample group were compared to find their correlation with planting years.【Results】Alpha diversity analysis showed that with the increase of continuous cropping years, both Shannon index and Chao1 index first decreased and then increased. At the phylum level, Acidobacteria, Proteobacteria, and Actinobacteria were dominant phyla. At the genus level, the dominant bacterial genera were an unclassified genus in the phylum Acidobacteria, the genus Sphingomonas, and the genus Acidophilus. The bacterial community composition of lavender soil with different ages was similar, but the relative abundance varied. Beta diversity also showed significant changes compared to unplanted soil, with the first and fifth years being similar, and both showing significant differences compared to those of the third year.【Conclusion】The continuous cultivation of lavender causes changes in the bacterial community structure and diversity of rhizosphere soil.

Key words: lavender; continuous cropping obstacle; high throughput sequencing; bacterial diversity; community structure

摘要：

【目的】探究不同连作年限薰衣草根际土壤细菌群落结构和多样性特征以及演变规律,为薰衣草土壤科学管理及增产提供参考。【方法】采集新疆伊犁哈萨克自治州霍城县不同连作年限以及未种植薰衣草土壤样品各3组,对16S rDNA序列进行 Illumina高通量测序。比较各样品组细菌多样性和群落分布规律及与种植年限的关联。【结果】随着连作年限的增加Shannon 指数和 Chao1指数均先降低后增高。在门水平上,酸杆菌门、变形菌门、放线菌门为优势菌门。在属水平上优势菌属为酸杆菌门中未被分类的一个属、鞘氨醇单胞菌属和嗜酸性杆菌属,不同年限的薰衣草土壤间细菌群组成相近,但相对丰度有所差异。Beta多样性与未种植土壤相比亦发生显著变化,其中第1年与第5年相似,均与第3年有较大差异。【结论】薰衣草连作种植造成根际土壤细菌群落结构和多样性改变。

关键词: 薰衣草, 连作障碍, 高通量测序, 细菌多样性, 群落结构

CLC Number:

S182

ZHANG Xiaole, Wueren Ahebieerdi, Entemake Bulatibai, Muguli Muhaxi, YUAN Qingqing, BAI Ru. Changes in bacterial diversity and community structure in rhizosphere soil of lavender with different continuous cropping years[J]. Xinjiang Agricultural Sciences, 2025, 62(3): 706-714.

张晓乐, 吾尔恩·阿合别尔迪, 恩特马克·布拉提白, 木古丽·木哈西, 袁青青, 白茹. 薰衣草不同连作年限根际土壤细菌多样性及群落结构的变化[J]. 新疆农业科学, 2025, 62(3): 706-714.

Figures/Tables 7

References 52

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样本 Sample	原始序列数 Raw tags	有效序列数 Effective tags	有效序列比例 Effective(%)	OTU数量 Number of OTU	覆盖度 Goods-coverage
L₀	95 166±11 883^a	66 833±2 505^a	71.12±7.26^a	3 701±42^a	0.990^ab
L₁	90 524±9 124^a	68 385±2 469^a	77.07±9.45^a	3 869±218^a	0.990^a
L₃	91 256±225^a	63 631±2 447^a	69.56±2.85^a	3 345±140^b	0.991±0.001^b
L₅	84 562±6 163^a	64 800±2 678^a	77.4±5.98^a	3 810±149^a	0.989^a

样本 Sample	原始序列数 Raw tags	有效序列数 Effective tags	有效序列比例 Effective(%)	OTU数量 Number of OTU	覆盖度 Goods-coverage
L₀	95 166±11 883^a	66 833±2 505^a	71.12±7.26^a	3 701±42^a	0.990^ab
L₁	90 524±9 124^a	68 385±2 469^a	77.07±9.45^a	3 869±218^a	0.990^a
L₃	91 256±225^a	63 631±2 447^a	69.56±2.85^a	3 345±140^b	0.991±0.001^b
L₅	84 562±6 163^a	64 800±2 678^a	77.4±5.98^a	3 810±149^a	0.989^a

样本 Sample	Shannon指数 Shannon index	Simpson指数 Simpson index	Chao1指数 Chao1 index	Ace指数 Ace index
L₀	9.801±0.113^ac	0.997±0.001^a	3 635.526±43.744^ab	3 726.847±67.421^a
L₁	9.680±0.267^ac	0.997±0.002^ab	3 854.589±342.052^b	3 907.551±395.014^a
L₃	9.282±0.357^b	0.995±0.002^b	3 359.662±184.162^a	3 387.528±146.537^b
L₅	9.639±0.025^bc	0.996±0.001^ab	3 757.002±138.369^b	3 815.712±163.43^a

样本 Sample	Shannon指数 Shannon index	Simpson指数 Simpson index	Chao1指数 Chao1 index	Ace指数 Ace index
L₀	9.801±0.113^ac	0.997±0.001^a	3 635.526±43.744^ab	3 726.847±67.421^a
L₁	9.680±0.267^ac	0.997±0.002^ab	3 854.589±342.052^b	3 907.551±395.014^a
L₃	9.282±0.357^b	0.995±0.002^b	3 359.662±184.162^a	3 387.528±146.537^b
L₅	9.639±0.025^bc	0.996±0.001^ab	3 757.002±138.369^b	3 815.712±163.43^a