Xinjiang Agricultural Sciences ›› 2023, Vol. 60 ›› Issue (12): 2869-2877.DOI: 10.6048/j.issn.1001-4330.2023.12.002
• Crop Genetics and Breeding·Germplasm Resources·Molecular Genetics·Soil Fertilizer • Previous Articles Next Articles
LIU Chenxi1(), ZHU Yuting1, ZHOU Qiang1, CHEN Jin1, ZHAO Wenjie1, ZHENG Kai1,2(
)
Received:
2023-04-09
Online:
2023-12-20
Published:
2024-01-03
Correspondence author:
ZHENG Kai(1988-), male, Xinyang, Henan Province, associate professor, doctoral and master tutor, research direction: cotton molecular breeding, (E-mail)Supported by:
刘晨曦1(), 朱雨婷1, 周强1, 陈瑾1, 赵文杰1, 郑凯1,2(
)
通讯作者:
郑凯(1988-),男,河南信阳人,讲师,博士,硕士生导师,研究方向为棉花分子育种,(E-mail)zhengkai555@126.com
作者简介:
刘晨曦(2001-),男,山东临沂人,研究方向为种子科学与工程,(E-mail)1176666997@qq.com
基金资助:
CLC Number:
LIU Chenxi, ZHU Yuting, ZHOU Qiang, CHEN Jin, ZHAO Wenjie, ZHENG Kai. Cloning and bioinformatics analysis of β-carotene isomerase GbD27-6 gene in sea island cotton[J]. Xinjiang Agricultural Sciences, 2023, 60(12): 2869-2877.
刘晨曦, 朱雨婷, 周强, 陈瑾, 赵文杰, 郑凯. 海岛棉β-胡萝卜素异构酶GbD27-6基因克隆及表达分析[J]. 新疆农业科学, 2023, 60(12): 2869-2877.
基因名称 Gene name | 引物名称 Primer name | 引物序列 Primer sequence(5'-3') |
---|---|---|
GbD27-6 | qGbD27-6F | ATGAAAATGATGCCTCTGGCTCTGA |
qGbD27-6R | TTACGCGTTTGGACATTTCATCACAT | |
UBQ7 | UBQ7-F | GACCTACACCAAGCCCAAGAAG |
UBQ7-R | TGAGCCCACACTTACCACAATAGT |
Tab.1 Primer sequence
基因名称 Gene name | 引物名称 Primer name | 引物序列 Primer sequence(5'-3') |
---|---|---|
GbD27-6 | qGbD27-6F | ATGAAAATGATGCCTCTGGCTCTGA |
qGbD27-6R | TTACGCGTTTGGACATTTCATCACAT | |
UBQ7 | UBQ7-F | GACCTACACCAAGCCCAAGAAG |
UBQ7-R | TGAGCCCACACTTACCACAATAGT |
软件名称 Software name | 网址 URL | 作用 Effect |
---|---|---|
Genbank | https://www.ncbi.nlm.nih.gov/genbank/ | CDS序列分析 |
ExPasy | | 理化性质预测 |
WoLF PSORT | | 亚细胞定位预测 |
SOPMA | | 蛋白质二级、三级结构预测 |
_automat.pl page=npsa_sopma.html | ||
SignaIP | | 蛋白质信号肽预测分析 |
TMHMM | | 蛋白质跨膜区分析 |
SMART | | 保守结构域分析 |
NetPhos | | 磷酸化位点分析 |
MEME | | motif分析 |
MEGA | | 多序列比对、进化树分析 |
Tab.2 Bioinformatics analysis software
软件名称 Software name | 网址 URL | 作用 Effect |
---|---|---|
Genbank | https://www.ncbi.nlm.nih.gov/genbank/ | CDS序列分析 |
ExPasy | | 理化性质预测 |
WoLF PSORT | | 亚细胞定位预测 |
SOPMA | | 蛋白质二级、三级结构预测 |
_automat.pl page=npsa_sopma.html | ||
SignaIP | | 蛋白质信号肽预测分析 |
TMHMM | | 蛋白质跨膜区分析 |
SMART | | 保守结构域分析 |
NetPhos | | 磷酸化位点分析 |
MEME | | motif分析 |
MEGA | | 多序列比对、进化树分析 |
Fig.2 Electrophoresis diagram of GbD27-6 gene bacterial fluid of sea island cotton Note:M:DL2000 DNA maker;2:Positive identification of the bacterial fluid of GbD27-6 gene
蛋白质折叠类型 Protein folding type | 折叠个数 Number of folds | 占比 Proportion(%) |
---|---|---|
无规则卷曲 Random coil | 133 | 49.08 |
α-螺旋 α-spiral | 88 | 32.47 |
延伸链 Extended chain | 37 | 13.65 |
β-转角 β-corner | 13 | 4.80 |
Tab.3 Secondary structure prediction of GbD27-6 protein
蛋白质折叠类型 Protein folding type | 折叠个数 Number of folds | 占比 Proportion(%) |
---|---|---|
无规则卷曲 Random coil | 133 | 49.08 |
α-螺旋 α-spiral | 88 | 32.47 |
延伸链 Extended chain | 37 | 13.65 |
β-转角 β-corner | 13 | 4.80 |
Fig.12 Expression of GbD27-6 gene of Gossypium barbadense in different tissues of floral organs Note:*,** and*** mean significant differences among different treatments of same gene at the 0.05, 0.01 and 0.001 probability levels,respectively,the same as below
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