Analysis of DNA Damage Repair Related Genes in Drought Stress Cotton Transcriptome

doi:10.6048/j.issn.1001-4330.2017.11.005

Abstract

Abstract: 【Objective】 The study aims to investigate the expression of DNA damage repair related genes in cotton under drought stress, and to explore the correlation between the expression of DNA damage repair related genes and drought resistance at the whole level.【Method】2.5% PEG 6000 was used to treat cotton, and transcriptome was sequenced using RNA-Seq technology of cotton seedlings under drought stress.【Result】A total of 51 genes with differentially expressed DNA damage were filtered from the transcripts of cotton drought stress response, among which there were 23 differentially up-regulated expressed genes and 28 differentially down-regulated expressed genes, which showed that drought stress could affect the expression of DNA repair related genes in cotton. 4 genes were selected by bioinformatics analysis and verification results showed that the expression of qRT-PCR, HMGB1, recA1, UDGs amplitude and GMP synthase gene displayed some differences, but the gene expression trends were consistent, showing that cotton transcriptome sequencing results verified by qRT-PCR were reliable.【Conclusion】The DNA damage repair related genes may be related to drought stress, so drought stress could affect the expression of DNA damage related genes.

Key words: cotton; drought stress; transcriptome sequencing; DNA damage repair related gene; differential expression

摘要： 【目的】研究干旱胁迫下,棉花 DNA 损伤修复基因的表达情况,从整体水平探讨棉花DNA损伤修复相关基因表达与抗旱的相关性。【方法】用2.5%PEG6000处理棉花幼苗,利用RNA-Seq技术对干旱胁迫下棉花幼苗的转录组进行测序。【结果】从棉花干旱胁迫响应的转录组中,筛选获得差异表达的 DNA损伤修复相关基因共51个,其中差异表达的上调基因23个,差异表达的下调基因28个,干旱胁迫能够影响棉花DNA损伤修复相关基因的表达。选取4个差异基因进行生物信息学分析及qRT-PCR验证,HMGB1、recA1、UDGs和GMP synthase基因的表达变幅有一定的差异,但基因的表达趋势一致,棉花转录组测序结果通过qRT-PCR验证是可靠的。【结论】DNA损伤修复相关基因可能与干旱胁迫有一定的相关性,干旱胁迫能够影响棉花DNA损伤修复相关基因的表达。

关键词: 棉花, 干旱胁迫, 转录组测序, DNA损伤修复相关基因, 差异表达

CLC Number:

S562
Q756

BAO Qiu-juan, ZHANG Li-li, Hainar Wulazibai, ZHANG Fu-chun. Analysis of DNA Damage Repair Related Genes in Drought Stress Cotton Transcriptome[J]. Xinjiang Agricultural Sciences, 2017, 54(11): 1999-2005.

包秋娟, 张丽丽, 海那尔·乌拉孜巴依, 张富春. 干旱胁迫棉花转录组DNA损伤修复相关基因的分析[J]. 新疆农业科学, 2017, 54(11): 1999-2005.

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