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    20 November 2017, Volume 54 Issue 11 Previous Issue    Next Issue

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    Effects of Fire Severity on Niche of Main Species in the Coniferous Broad-leaved Forest in Kanas, Xinjiang
    LIU Jing, PAN Cun-de, YU Ge-bi, LI Gui-hua, LIU Xiao-ju, CUI Qian, YANG Yu-ping
    2017, 54(11): 1961-1971.  DOI: 10.6048/j.issn.1001-4330.2017.11.001
    Abstract ( )   PDF (1149KB) ( )  
    【Objective】 Through the analysis of niche transition under different fire severity in a coniferous broad-leaved forest in Kanas to explain the mechanism of succession of plant community fire disturbance from the niche perspective, this project aims to provide scientific basis for the Kanas taiga forest community species diversity conservation and sustainable forest management.【Method】In this paper, the value of ecological niche width and niche overlap of the main species in tree layer, shrub layer and herbaceous layer under different fire severity were quantitatively analyzed by the ecological niche width of Shannon-Wiener and the niche overlap of Pianka.【Result】The niche width of Pinus sibirica and Elymus sibiricus had significant differences (P < 0.05) under different fire severity, the niche breadth of Betula pendula, Vaccinium hirtum, Cerastium pauciflorum and other nine main species had slight difference(P > 0.05), the niche width of Larix sibirica, Lonicera caerulea, Carex polyphylla and other plants had significant difference (P < 0.05) between any fire disturbance intensity and other two kinds of fire disturbance intensity. Tree layer niche overlap value of main plant species of forest community reached the maximum in mild fire disturbance intensity, but there was little difference between moderate fire disturbance intensity. The niche overlap values of main plant species in shrub layer and herb layer were the largest under moderate fire disturbance intensity. The species of niche overlap value more than 0.8 under the moderate fire disturbance intensity was the largest and the least under the weak fire severity.【Conclusion】Different natural fire severity can lead to the ecological niche change of the main species in the coniferous broad-leaved forest of the Kanas by the environment heterogeneity and it is also affected by the biological characteristics of plants.
    Effect of Drip Irrigation Pattern and Planting Density on Leaf Senescence of Cotton
    MA Hui, NIU Yu-ping, XIA Jun, CHEN Zong-kui, LUO Hong-hai
    2017, 54(11): 1972-1982.  DOI: 10.6048/j.issn.1001-4330.2017.11.002
    Abstract ( )   PDF (1944KB) ( )  
    【Objective】 To explore the regulation of drip irrigation pattern and planting density on the cotton yield-forming stage of leaf senescence and dry matter production and provide evidence for regulation drip irrigation of cotton premature senescence and increase yield. 【Method】Used Xinluzao 45 as the experimental material, field experiments were conducted with two drip irrigation patterns (I1, 600 nm and I2, 450 nm) and three levels of planting densities (D1, 12×104 plant/hm2; 24×104 plant/hm2; 36×104 plant/hm2). The leaf area index, chlorophyll content, protective enzyme of cotton and dry matter accumulation were measured.【Result】Compared with the conventional drip irrigation, limited drip irrigation treatment of leaf area index(LAI) decreased by 17.8%, but superoxide dismutase activity (SOD) and peroxidase activity (POD) increased by 8.4%, 44.7%, 12.9%, respectively. There was not significant difference between the two treatments in dry matter accumulation. With the increase of planting density, LAI, dry matter accumulation as well soluble protein (Pr), malondialdehyde (MDA) and chlorophyll content at full boll stage showed a trend of increase gradually. Correlation analysis showed that the SOD was very significant positive correlation between LAI and (r = 0.485**), LAI and reproductive organs dry and total dry matter were extremely significant positive correlation (r = 0.721**, r = 0.721**). Under limited drip irrigation condition, the high density treatment enhanced the leaves chlorophyll content, SOD activity, POD activity and Pr content after full boll stage, and extended LAI maximum duration, which finally achieved the highest total and reproductive dry matter accumulation.【Conclusion】Therefore, under the condition of limited drip irrigation a modest increase in planting density can be used as effective control measures to delay the cotton senescence and increase the production in arid areas.
    Effects of Water-Nitrogen Coupling on Growth and Yield of Cotton under Mulch Drip Irrigation
    HE Huai-jie, WANG Zhen-hua, ZHENG Xu-rong, ZHANG Jin-zhu, LI Wen-hao, WU Qiang
    2017, 54(11): 1983-1989.  DOI: 10.6048/j.issn.1001-4330.2017.11.003
    Abstract ( )   PDF (1240KB) ( )  
    【Objective】 To study the effects of water and nitrogen coupling on the growth and yield of cotton under mulch drip irrigation in Shihezi area of Xinjiang, pot experiment was designed.【Method】Nongfeng No.133, an early-maturing variety commonly cultivated in the region was used as the test material under drip-irrigated condition in a pot experiment that was completely randomized with two-factor and three-level treatments. Three irrigation levels were set: 4,350, 5,250 and 6,150 m3/hm2 (labeled W1, W2, W3) and three nitrogen application levels were set: 300, 500, 700 kg/hm2 (labeled F0.6, F1.0, F1.4). The effects of different treatments on plant height, leaf area index, dry matter accumulation and yield of cotton were analyzed and measured.【Result】The results showed plant height and leaf area index were increased with the increase of irrigation amount under the condition of mulch drip irrigation. At the same irrigation level, with the increase of the amount of nitrogen, the index showed that the trend increased at first and then decreased and the maximum value arrived at F1.0, however, excessive fertilization inhibited the growth of cotton to some extent.【Conclusion】Based on the effects of water-nitrogen coupling on the growth of drip-irrigated cotton,water and nitrogen fertilizer were recommended to be applied in the amounts of 5,250 m3/hm2 and 500 kg/hm2,respectively in the Shihezi area in order to achieve the highest yield.
    Effects of Nitrogen Application Rate on Canopy Characteristics of Machine Harvesting Cotton under Drip Irrigation during Different Growth Stages
    CHEN Bao-yan, MA Hong-hong, NIU Xin-xiang, MA Xing-wang, YANG Tao, ZHU Jing-rong
    2017, 54(11): 1990-1998.  DOI: 10.6048/j.issn.1001-4330.2017.11.004
    Abstract ( )   PDF (1236KB) ( )  
    【Objective】 The project aims to improve the canopy structure and the yield and increase the quality of machine harvesting cotton by adjustin the strategy nitrogen application rate.【Method】Different amounts of nitrogen fertilizer were applied at different growth periods based on the same basic fertilizer. Canopy characteristics of cotton was monitored with the CI-110 canopy instrument.【Result】The amount of nitrogen regulation in bud stage, LAI index, MFIA index and T index were increased with the increase of amount of nitrogen fertilizer, TC index and K index decreased with the increase of amount of nitrogen fertilizer; In flowering period, LAI index and K index first increased and then decreased with the increase of application amount of nitrogen, MFIA index and T index decreased first and then increased with the amount of nitrogen increased, TC index increased first and then tended to be stable with the increase of the amount of nitrogen; In full boll stage, with the increase of nitrogen, LAI index increased first and then decreased, and the MFIA index increased with it. What was more, TC index increased first and then decreased, T index decreased first and then increased, K index increased first and then decreased.【Conclusion】At the full boll stage, nitrogen regulation has a good effect on regulating LAI index; The differences of MFIA index, K index and T index in the bud stage, flowering stage and full boll stage are not so great. The TC index value of bud stage treatment was higher than those of full flowering stage and full boll stage.
    Analysis of DNA Damage Repair Related Genes in Drought Stress Cotton Transcriptome
    BAO Qiu-juan, ZHANG Li-li, Hainar Wulazibai, ZHANG Fu-chun
    2017, 54(11): 1999-2005.  DOI: 10.6048/j.issn.1001-4330.2017.11.005
    Abstract ( )   PDF (1196KB) ( )  
    【Objective】 The study aims to investigate the expression of DNA damage repair related genes in cotton under drought stress, and to explore the correlation between the expression of DNA damage repair related genes and drought resistance at the whole level.【Method】2.5% PEG 6000 was used to treat cotton, and transcriptome was sequenced using RNA-Seq technology of cotton seedlings under drought stress.【Result】A total of 51 genes with differentially expressed DNA damage were filtered from the transcripts of cotton drought stress response, among which there were 23 differentially up-regulated expressed genes and 28 differentially down-regulated expressed genes, which showed that drought stress could affect the expression of DNA repair related genes in cotton. 4 genes were selected by bioinformatics analysis and verification results showed that the expression of qRT-PCR, HMGB1, recA1, UDGs amplitude and GMP synthase gene displayed some differences, but the gene expression trends were consistent, showing that cotton transcriptome sequencing results verified by qRT-PCR were reliable.【Conclusion】The DNA damage repair related genes may be related to drought stress, so drought stress could affect the expression of DNA damage related genes.
    Study on K Absorption, Distribution and Utilization of Cotton under Different Planting Densities
    LOU Shan-wei, WANG Da-guang, MA Teng-fei, Tuoheti Aimait, BIAN Yang, ZHANG Peng-zhong
    2017, 54(11): 2006-2012.  DOI: 10.6048/j.issn.1001-4330.2017.11.006
    Abstract ( )   PDF (1103KB) ( )  
    【Objective】 To explore the difference between cotton plant potassium absorption, distribution and utilization under different cotton planting densities and clarify the relationship between potassium and cotton yield.【Method】The experiment was conducted to study the distribution and variation of potassium content and organ potassium content in plants by setting 5 (A1-A5) different planting densities.【Result】The potassium absorption and utilization was influenced by density, with the increase of density, plant K content decreased gradually, but in group accumulation, A3 treatment's content was the highest before boll opening, reaching 483 kg/hm2. Potassium distribution ratio in different organs, early flowering stem and leaf potassium K ratio accounted for more than 70%, and the highest proportion of stem, bud to boll opening, the lowest proportion; leaf potassium ratio became lower and the highest with the density increasing trend, while the bell potassium content in A3 treatment was the highest, up to 29.36. Density was the main factor affecting the yield. With the increase of density, the yield was first increased and then decreased, In A3 treatment, the highest lint yield was 3,112.11 kg/hm2 and the correlation coefficient was 0.979,2, but it was also consistent with the higher trend of the group potassium accumulation at the stage of boll opening and rapid accumulation of A3 value.【Conclusion】The correlation between density and yield is two times, and it can affect the absorption and utilization of potassium, but also it is also related to cotton yield, K accumulation rate, K content in the population, potassium content in reproductive organs.
    Effect of Drip Irrigation and Fertilizer Coupling on Peanut (Arachis hypogaea L.) Dry Matter Accumulation and Its Yield
    LIU Xin-ting, WANG Juan, HOU Xian-fei, CHEN Yue-hua, LAN Hai-yan, LI Qiang
    2017, 54(11): 2013-2021.  DOI: 10.6048/j.issn.1001-4330.2017.11.007
    Abstract ( )   PDF (1577KB) ( )  
    【Objective】 In order to study the effect of irrigation and fertilizer coupling on peanut dry matter accumulation dynamic, physiological index and yield, in this experiment, Huayu 33 was used as fertilizer to analyze the effect of water and fertilizer coupling at different growth stages.【Method】The experiment was divided into five parts: no fertilizer(A control), seedling fertilizing (B), top dressing at flowering stage (C), floricome period fertilizing (D), pod setting period fertilizing (E) according to the different fertilizer periods. The quantity of fertilizer was the same with each other: N 180 kg, 90 kg P2O5, K2O 45 kg and CaO 30 kg for per hectare.【Result】During pod setting period, fertilization promoted the growth of peanut plant height; The ratio of root to shoot of peanut was the highest at the beginning period of flowering, which showed that fertilization in this period was conducive to the reproductive growth and dry matter accumulation of peanut. At the same time, according to the table of area yield, at the beginning of flowering period, fertilizing the peanut, peanuts area yield was the highest, reaching 527.93 kg; The analysis of peanut per plant and main agronomic characters showed the stem height, lateral branch length, the total number of branches, number of fruit, full fruit number per plant, hundred kernel weight and other agronomic traits had the impact on individual plant productivity; fertilization at floricome period was conducive to the growth of the agronomic traits and to improvement of peanut productivity per plant.【Conclusion】In conclusion, water and fertilizer treatments have different effects on peanut at different developmental stages, and fertilization at first flowering stage can increase peanut yield per mu and create more revenue.
    Effect of Occurrence Grades of Verticillium Wilt on Yield and Fiber Quality in Island Cotton
    DAI Cui-rong, LIAN Wen-ming, TAI Hong-zhong, LU Jin-bao, WU Bo, WANG Xian-li, HE Mei-qiu, WU Gang, ZHAO Jing
    2017, 54(11): 2022-2027.  DOI: 10.6048/j.issn.1001-4330.2017.11.008
    Abstract ( )   PDF (1039KB) ( )  
    【Objective】 To study the effect of verticillium wilt on yield and fiber quality in Island Cotton Xinhai No. 42 in the hope of providing a theoretical basis for the further prevention and control of verticillium wilt in Island Cotton.【Method】With the island cotton Xinhai No.42 as the research object, all samples of cotton stalk in field desease mursery conditon were cut on October 30th and the occurrence grades were classified and the yield tested and analysed.【Result】The effect of the of cotton verticillium wilt on yield showed that the boll number, boll weight, lint decreased with the increase of the grades of cotton verticillium wilt; the effect of the occurrence grades of cotton verticillium wilt on fiber quality showed that the differences between healthy plant and grade IV diseased plants were significant in upper half mean length, HVI strength, micronaire value, maturity, uniformity and short fiber.【Conclusion】The effect of occurrence grades of cotton verticillium wilt on Yield in Xinhai 42 was great, which showed that the disease had different degrees of effect on different indexes such fiber quality of the plant.
    Effects of Combined Application of Nitrogen, Phosphorus and Potassium Fertilizer on Agronomic Traits and Yield of Barley in Dryland
    WANG Xian, NIE Shi-hui, ZHANG Jin-shan, REN Yi, GENG Hong-wei, ZHANG Jian-ping, XU Qi-jiang, DONG Qing-guo, FANG Fu-rong
    2017, 54(11): 2028-2035.  DOI: 10.6048/j.issn.1001-4330.2017.11.009
    Abstract ( )   PDF (1138KB) ( )  
    【Objective】 To explore the optimal ratio of fertilization to barley, build fertilization model of dry barley and provide the basis for fertilization zoning and fertilizer formula design, we study the effect of different NPK treatments on yield and agronomic traits of barley in the dry conditions.【Method】Under the condition of dry farming, using barley Ganpi No. 7 as the test material, the three elements, two effect equations of nitrogen, phosphorus, potassium were fitted with the optimum design of 3 factors, 4 levels and 14 treatments.【Result】The effects of nitrogen, phosphorus, and potassium on barley yield were N> K2O >P2O5; plant height, panicle length, grains per spike, 1,000-grain weight and panicle number were significantly changed with the growth of nitrogen fertilization in barley, 1,000-grain weight and panicle number in barley fertilizer changed significantly with the increasing of P fertilizer application, and plant height, panicle length, spikelet number, grains per spike and panicle number varied significantly with increasing K fertilizer application under dry condition. The amount of nitrogen, phosphorus and potassium in the production were controlled within 95.85, 120.6 and 14.96 kg/hm2.【Conclusion】Under the low fertility condition, nitrogen inputs should be first considered in fertilizer inputs, phosphorus and potassium are determined by nitrogen; N∶P2O5∶K2O with 1∶0.83∶0.02 is appropriate for barley in dryland.
    Effects of Drip Irrigation Frequency on Field Microclimate Characteristics of Winter Wheat Population
    HUANG Zhen-jiang, CHEN Hui, WANG Ji-chuan, PAN Xue-jiao, ZHENG Lei
    2017, 54(11): 2036-2045.  DOI: 10.6048/j.issn.1001-4330.2017.11.010
    Abstract ( )   PDF (1429KB) ( )  
    【Objective】 Under the drip irrigation plot test condition, the soil water distribution and field microclimate characteristics of winter wheat under different drip frequencies in the oasis area of Southern Xinjiang were studied in the hope of providing basis for making winter wheat drip irrigation system.【Method】Based on the unified winter irrigation of 900 m3/hm2, after the spring, the drip irrigation amount was 4,050 m3/hm2 using three irrigation frequencies: low frequency P1 was 4, medium frequency P2 was 7, high frequency P3 was 10.【Result】Soil water distribution was greatly affected by drip frequency. The average soil moisture contents of 0-50 cm soil layer in high frequency, medium frequency and low frequency irrigation treatments were 21.97%, 20.04% and 19.76%, respectively, and the soil moisture contents of 0-30 cm layer were more stable when treated with high frequency; In the growth period, the average soil temperature of high frequency irrigation treatment was 0.40℃ and 0.83℃ lower than those of medium and low frequency irrigation treatments the average temperature in the canopy decreased by 0.37℃ and 1.15℃, canopy leaf temperature decreased by 0.42 ℃ and 1.30℃, relative humidity in the canopy increased by 1.11 and 2.28 percentage points, canopy light interception rate increased by 0.025,6 and 0.055,1. Compared with medium frequency and low frequency irrigation treatments, the yield and irrigation water use efficiency of high frequency irrigation treatment increased by 1.36% and 20.47%. 【Conclusion】High frequency drip irrigation in winter wheat field can improve field microclimate and increase yield and water use efficiency in the oasis area of Southern Xinjiang. In practice, 7-10 times drip irrigation model is appropriate after spring.
    Species and Distribution of Cotton Root and Stem Diseases in Eastern Xinjiang Based on Molecular Detection
    Mayinuer Mijiti, Zumina, Sajida Aizizi, GUO Qing -yuan
    2017, 54(11): 2046-2053.  DOI: 10.6048/j.issn.1001-4330.2017.11.011
    Abstract ( )   PDF (1689KB) ( )  
    【Objective】 Clarifying species and distribution of cotton root and stem diseases in eastern Xinjiang can provide a basis for effective prevention and control of such diseases in the local area.【Method】The reported specific primers of the 4 species pathogens (Fusarium oxysporum, Rhizoctonia solani, verticillium dahliae and Fusarium verticilliodes) which cause the root and stem disease of cotton are used for the specific verification, and carrying out PCR detection for the mixed disease samples of the collected according to different villages (townships) from the cotton areas in Eastern Xinjiang.【Result】In the tested system with sensitivity of 0.5 ng/μL, 3 species of 4 pathogenic bacteria were detected from the 22 mixed samples of cotton infected plants from the Eastern Xinjiang. They were respectively Fusarium oxysporum, Rhizoctonia solani and verticillium dahliae;Among them, Fusarium oxysporum had the highest detection rate,Rhizoctonia solani was the second, V. dahliae dad the lower detection rate. But, the detection rate ofFusarium verticilliodes was 0% in the 22 mixed samples.【Conclusion】The detection results indicated that the Fusarium wilt,Rhizoctonia damping-off and Verticillium yellow wilt were the main root and stem diseases of cotton in Eastern Xinjiang; Among which, Fusarium wilt is the most common one, followed by Rhizoctonia damping-off, and the incidence of Verticillium wilt is relatively low. In Eastern Xinjiang, red rot has not occurred or distribute very little.
    A Preliminary Study on Resistance of Hami Melon Varieties to Acidovorax avenae subsp. citrulli in Turpan Area
    MAO Liang, JI Yan-ling, ZHANG Juan, WANG Xuan-cang, PAN Wei-ping, ZHANG Yi-he, WANG Xiao-dong
    2017, 54(11): 2054-2059.  DOI: 10.6048/j.issn.1001-4330.2017.11.012
    Abstract ( )   PDF (1960KB) ( )  
    【Objective】 This project aims to provide the theoretical basis for the prevention and breeding of bacterial fruit blotch of Hami melon through the study on the resistance to Acidovorax avenae subsp. Citrulli in Turpan,【Method】10 Hami melon varieties planted in Turpan area were used as materials,and the disease index of resistance to Acidovorax avenae subsp. citrulli was investigated by artificial inoculation at the seedling stage. At the same time, the stomatal and leaf hair number of seedlings leaf surface between different resistant cultivars were compared and the defense enzymes PPO and POD in seedling leaves were measured.【Result】Among the 10 cultivars, there were no immune varieties, Xizhoumi No. 25 and Imperial Concubine showed the middle resistance, Xinmi No. 36 showed a high susceptibility, and the other seven varieties showed a middle susceptibility; There were no significant differences in leaf hair number between susceptible varieties Xinmi No. 36 and middle susceptible varieties 9818 (green leaf trichomes). Leaf hair number of the middle resistance Xizhoumi No.25 was 1.68 times that of Xinmi No. 36. The number of stomata of Xizhoumi No. 25 was 1.42 times that of 9818 (green leaf trichomes) and 1.91 times that of Xinmi No.36. The PPO enzyme activities of different resistant varieties showed susceptible varieties > middle susceptible varieties > resistant varieties, POD enzyme activity showed susceptible varieties > middle susceptible varieties > resistant varieties.【Conclusion】Different Hami melon varieties have significant difference resistance to Acidovorax avenae subsp. Citrulli, and no immune and high resistant varieties among them have been found. Xizhoumi No. 25 and Imperial Concubine show middle resistance, Xinmi No. 36 is a high susceptible variety, other varieties are middle and high susceptible varieties. There was a positive correlation between disease resistance and the number of leaf hair and stomata, showing positive correlation trend. The PPO enzyme showed a downward trend, which was negatively related to disease resistance, and POD enzyme showed an upward trend, which was negatively related to disease resistance of varieties.
    Identification of Pathogens Causing Leaf Spot Disease at Cotton Seedling Stage in Shihezi Area
    LI Ying-cheng, ZHANG Guo-li, YANG Chao, REN Yu-zhong, LI Zhuo, ZHANG Li, LI Guo-ying
    2017, 54(11): 2060-2066.  DOI: 10.6048/j.issn.1001-4330.2017.11.013
    Abstract ( )   PDF (2359KB) ( )  
    【Objective】 The aim of the study is to verify the pathogens that cause cotton seedling leaf spot in Shihezi area.【Method】120 samples of cotton leaf spot were collected from Shihezi area, and 105 strains of Alternaria spp were isolated and purified by conventional method, and 15 representative strains were identified through survey of morphological characteristics, test of pathogenicity and sequence analysis of histone 3 gene.【Result】The selected 15 representative strains were pathogenic. The pathogens were preliminarily identified as A.alternata and A.tenuissima according to morphological characteristics, respectively. Based on histone 3 gene sequence analysis, the results showed that the similarity of nucleotide homogeneity of A.alternata (AF404620, KF997067 and KR866858) was 99% and A.tenuissima (KT384348) was more than 99%, respectively. 【Conclusion】The pathogens causing cotton seedling leaf spot are A.alternata and A.tenuissima in Shihezi in 2016.
    Diversity of the endophytes and screening of antagonistic microbes in Garlic varieties from different producing areas
    CHU Min, ZHANG Li-juan, LIU Xiao-Jing, ZHU Jing, WANG Wei, GU Mei-ying, XIE Yu-qing, ZHANG Zhi-dong
    2017, 54(11): 2067-2074.  DOI: 10.6048/j.issn.1001-4330.2017.11.014
    Abstract ( )   PDF (1427KB) ( )  
    【Objective】 In order to investigate diversity of the endophytes in Garlic varieties from different producing area, and obtain antagonistic microbes.【Method】Garlic varieties, white garlic and red garlic from different producing areas were sampled. And then, Biolog Eco method was employed to investigate metabolic characteristics of endophytes. Meanwhile, Endophytes were isolated and antagonistic bacteria to Rhizoctonia soleni,Fusarium oxysporumf,Penicillium expansum,Escherichia coli and Staphyloccocus aureus were screened.【Result】Low metabolic activity of endophytes in Garlic were determined, and some differences in the utilization and diversity in Garlic varieties from different producing areas were analyzed. 45 endophytes were isolated which were involved in 15 species belonging to genus Bacillus, genus Paenibacillus , genus Streptomyces , genus Nocardiopsis , and genus Dietz . Isolates in Genus Bacillus were dominant in Garlic endophytes, accounted for more than 85%, but there were differences in the distribution of species. Additionally, lots of antagonistic bacteria were obtained. 【Conclusion】Diversity of the endophytes in Garlic varieties from different producing area were all poor, but effective resource of antagonistic microbes needs further explore.
    Influences of Two Kinds of Coarse Cereals on Flour Quality Characteristics
    MAO Hong-yan, XU Xin, YU Ming
    2017, 54(11): 2075-2085.  DOI: 10.6048/j.issn.1001-4330.2017.11.015
    Abstract ( )   PDF (1436KB) ( )  
    【Objective】 This project aims to study the influences of wheat flour and millet flour on flour quality by using different proportion of wheat flour and coarse flour (chickpeas, millet) as main raw materials.【Method】The rheological properties, gelatinization properties and the drop value of the dough of different mixed proportions of mixed cereals were analyzed and the quality evaluation of the steamed bread made of the coarse flour was evaluated.【Result】Results showed that the wheat flour mixed with grain flour of 5%,10%,15% and 20% , the mix powder of each index is on the decline, and with the increase of adding amount of the rheological properties of dough and pasting properties, falling number and wet gluten content were decreased, the quality characteristics of flour were changed by addition of grains . By adding grain flour although can make the structure of the steamed bread, specific volume declined slightly, the color, taste and smell, make the steamed bread which has contained unique aroma and taste.The results indicated that adding 15% of the grain powderin the wheat flour, the effect on the dough quality characteristics and steamed bread was not obvious.【Conclusion】The best comprehensive evaluation of steamed bread is when wheat flour is added with 15% cereal powder and the suitable ration of chickpea and millet flour should be chosen in combination with the quality of the final processed products.
    Isolation and identification of Poly (butylene adipate-co-terephthalate) -degrading Bacteria
    HUO Xiang-dong, GAO Yan, LIN Qing, ZENG Jun, ZHANG Tao, CHU Min, YANG Hong-mei, SHI Ying-wu, WANG Bin, SUN Jiu-sheng, WANG Jin-xin
    2017, 54(11): 2086-2091.  DOI: 10.6048/j.issn.1001-4330.2017.11.016
    Abstract ( )   PDF (1805KB) ( )  
    【Objective】 To isolate poly (butylene adipate-co-terephthalate)-degrading bacteria and study the capability of biodegradation of the bacteria.【Method】Using PBAT powder as the sole carbon source to isolate Poly (butylene adipate-co-terephthalate)-degrading bacteria from the soil which was covered with PBAT mulch. The strain was identified by 16S rDNA sequence comparative analysis. The degradation capability of strain in the liquid medium was evaluated by weight loss method and scanning electron microscope.【Result】1 Poly (butylene adipate-co-terephthalate) - degrading bacteria was isolated and identified as Sphingopyxis ginsengisoli . In the liquid medium, degradation rate of PBAT reached up to 0.92% after 60 days.【Conclusion】The Sphingopyxis ginsengisoli strain XJSL2 can be used to recycle of PBAT mulch. There are still a lot of soil microorganisms able to degrade PBAT, This still needs further research.
    Rapid and Nondestructive Detection of the pH Value and Luminosity for Vacuum Packed Chilled Mutton by Using Near Infrared Reflectance Spectroscopy Technique
    QIU Yuan-yuan, ZHU Rong-guang, HUANG Kun-peng, FAN Zhong-jian, YAN Cong, MENG Ling-feng
    2017, 54(11): 2092-2099.  DOI: 10.6048/j.issn.1001-4330.2017.11.017
    Abstract ( )   PDF (1266KB) ( )  
    【Objective】 The purpose of this research is to solve the problems in the traditional determination of mutton freshness physicochemical indexes, such as the fussy operation, the necessary chemical reagents, and destruction and contact of samples and so on.【Method】The small tailed Han Sheep in Xinjiang was taken as the research object, and the near infrared reflectance spectroscopy (NIRS) technique was used to carry out the rapid and nondestructive detection of physicochemical indexes in vacuum packed chilled mutton during the storage. For the chemical index pH and the physical index luminosity (L*), the spectral pretreatment and the feature bands selection methods were adopted to optimize and simplify the prediction model of each index content.【Result】The optimal models of pH and L* were the multiple linear regression (MLR) models established with the feature bands, which were selected by the combination of genetic algorithm (GA) and successive projections algorithm (SPA) rather than the single method. The correlation coefficients of prediction sets were 0.91 and 0.91, and the root mean square errors of prediction sets were 0.13 and 1.91.【Conclusion】NIRS technique has the detection advantages of non-pollution, nondestructive, and simultaneous analysis of multiple indexes, and it can replace traditional detection methods to achieve rapid, nondestructive and accurate detection of freshness physicochemical indexes in meat.
    Biological Characteristics Evaluation and Principal Component Analysis of Different Onion Germplasm Resources
    ZHUANG Hong-mei, WANG Hao, HAN Hong-wei, WANG Qiang, LIU Hui-fang, YANG Sheng-bao
    2017, 54(11): 2100-2110.  DOI: 10.6048/j.issn.1001-4330.2017.11.018
    Abstract ( )   PDF (1146KB) ( )  
    【Objective】 The purpose of this paper is to evaluate the characters of different onion varieties, analyze the diversity and specificity, and screen out the suitable varieties which can express good agronomic traits and have preferred specific traits for the local people.【Method】Field experiments about different onion varieties were carried out in Anningqu and Fukang respectively. Moreover, agronomic traits of the introduced different onion varieties were also investigated. Furthermore, the agronomic traits of different onion varieties were analyzed via principal component analysis and correlation analysis.【Result】The results showed that Jinqiu-white, Chunyuan-red, Lufeng-white and Xiyanghong-white had good performance on biological characteristics and high yield among all the various characters, except the variety Yuyuan-red and Jinqiu-red. According to the results of correlation analysis, a significant correlation existed among the single bulb weight, the yield, the vertical diameter of the bulb and the bulb diameter. The leaf number was significantly correlated with stem diameter and the number of fleshy scales. The leaf length also had a significant correlation with plant height, width and leaf diameter. A negative significant correlation of wax powder between disc stem diameter and thick fleshy scales was also found.【Conclusion】Jinqiu-white,Chunyuan-red,Lufeng-white,Xiyanghong-white can be used as excellent germplasm resources for native onion varieties cultivar improvement.
    Effects of Different Doses of Melatonin on Duolang Sheep Pregnancy and Lambing Effect
    Aerman Haire, Aihemaitijiang Tuerxun, SONG Yu-kun, WANG Xu-guang, Nuribiyamu Maimaitituoheti, LIU Guo-shi, Abulizi Wusiman
    2017, 54(11): 2111-2117.  DOI: 10.6048/j.issn.1001-4330.2017.11.019
    Abstract ( )   PDF (1139KB) ( )  
    【Objective】 To study the influence of melatonin on Duolang sheep pregnancy and lambing effect, to select the optimal amount to improve the pregnancy rate and lambing rate and improve the reproduction rate. 【Method】This study selected 57 Duolang sheep ewes with similar age, parity and body condition from November 2016 to April 2017 to conduct experiments in the professional farmers' cooperatives in Luopu County of Hotan area. Sheep were randomly divided into three groups at the same time for the same period of estrus, and after the withdrawal of 36 hours, the test group I was injected melatonin 1 mL (5 mg), the test group II 2 mL (10 mg), the control group 0ml. And before the burying bolt (12 o'clock midday), bolt withdrawal (12 o'clock midday), 36 hours after the bolt withdrawal (12 o'clock midnight), the breeding (48 hours after embolization, serum withdrawal) of the five time periods for 20 days after the acquisition of its breeding, melatonin contents were determined and analyzed by radioimmunoassay; forty-fifth days after mating, they went through B-monitoring assay in three groups' pregnancy for the lambing rate; After the birth, the lambing rate of the three groups was compared.【Result】The test results showed that the pregnancy rate of test group I was significantly higher than that of the test group II and the control group (P < 0.05), there was no significant difference between test group II and control group (P > 0.05); As for the the lambing rate, comparison between the three groups demonstrated no significant difference (P > 0.05).【Conclusion】When treating Duolang sheep estrus, subcutaneous injection of 1 mL, 2 mL melatonin 36 hours after the withdrawal of thrombus can improve the pregnancy rate. The pregnancy rate of 1 mL injection amount is higher than that of 2 mL injection, but no significant effect is found on the lambing rate.
    Genetic Evolution Analysis of F gene of PPRV in Southern Xinjiang
    ZHANG Lu-yao, LI Fei, JIAO Hai-hong, ZHAO Li, LIU Yong-hong
    2017, 54(11): 2118-2129.  DOI: 10.6048/j.issn.1001-4330.2017.11.020
    Abstract ( )   PDF (2048KB) ( )  
    【Objective】 In order to provide a theoretical basis for the prevention and control of PPR in southern Xinjiang, the PPRV strains in 2014 was analyzed.【Method】The incidence of sheep in a sheep farm in Akesu Prefecture of southern Xinjiang was investigated. The clinical symptoms and pathological examination were performed. The PPRV F gene RT-PCR and F gene sequencing were carried out in the laboratory, and the sequence analysis of PPRV F gene was further carried out.【Result】PPRV F gene sequence analysis showed that the test China/XJYL/2013 in Xinjiang had the highest homology with domestic PPRV strainF gene nucleotide. Compared with the Foreign strains, the highest homology of nucleotide is India strains Izatnagar/94 and Izatnagar-94 strains; PPRV strain F gene phylogenetic tree analysis in this study showed that the relationship of this PPRV strain had recently evolved from Beijing PPRV strain and belonged to the same branch of Ili PPRV strain, which had close evolutionary relationship with foreign strains in India and Bangladesh.【Conclusion】There existed PPR epidemic in southern Xinjiang in 2014, and the epidemic strains and the domestic PPR before and after the outbreak of the PPR strains and the surrounding countries strains were relatively close to the evolution.
    Differences in Theileria equi Infection and Its Phylogenetic Development Analysis in Some Areas of Xinjiang
    LIU Shi-fang, GUO Qing-yong, WEN Xiu-xiu, ZHANG Meng-yuan, DANG Na-na, Alax Abing, Bayinchahan
    2017, 54(11): 2130-2137.  DOI: 10.6048/j.issn.1001-4330.2017.11.021
    Abstract ( )   PDF (1709KB) ( )  
    【Objective】 To understand the situation of Theileria equi and the genetic distance of local endemic strains strain of Theileria equi in Xinjiang.【Method】478 horse blood samples were collected from Southern and Northern Xinjiang, and Equine Theileriosis was investigated by PCR, the 18S rRNA gene was analyzed and the phylogeny tree was constructed based on Maximum Likelihood algorithm.【Result】The result showed that the infection rate in Northern Xinjiang was 13.96% (25/179), and the infection rate in Southern Xinjiang was 27.09% (78/299); It was thought that the strains in India, South Africa, Spain, Iran and other places clustered into one branch and the strains with the genes (MF398476, MF398477) and Swedish local strains clustered into another branch.【Conclusion】The infection rate of Equine Theileriosis between Northern and Southern Xinjiang displayed significant difference(P=0.001<0.05),but there was no significant difference(P>0.05)in different age groups. The amplified Theileria equi in Altay and Tuokexun endemic strains (MF398477, MF398476) and the local strains of Switzerland (KM046918.1) had the closest relationship. The test provided a data to support the control of Equine Theileriosis and the accurate classifying status of Theileria equi.
    Case Study on the Factors Influencing the Survival of Mutton Sheep Embryo Transfer
    GUO Yan-hua, LIU Chang-bin, WAN Peng-cheng, NI Jian-hong, WANG Li-min, ZHOU Ping, YANG Yong-lin, FANG Zhi-zhong, GU Hong-wei, FU Bing-zhe, LU Quan-sheng
    2017, 54(11): 2138-2145.  DOI: 10.6048/j.issn.1001-4330.2017.11.022
    Abstract ( )   PDF (1137KB) ( )  
    【Objective】 Multiple ovulation and embryo transfer (MOET) technology is an important technique for obtaining excellent germplasm resources, but many factors affect embryo survival and pregnancy, and a large amount of data is needed to support it. The purpose of this study is to investigate the influence of embryonic and receptor factors on survival after embryo transfer.【Method】A retrospective survey about embryonic and recipient factors on the survival rate of transferred embryo was done in our application in mutton sheep.【Result】These results showed that the remote transition of donor, recipient and fresh embryo were adverse to gestation than on site transition (P<0.01). There was no significant difference on pregnancy between embryonic cleavage and blastocyst stage(P>0.05), but the rate of pregnancy with two cell transplantation on the embryonic cleavage stage was the lowest. And it was the same to embryo survival and recipient pregnancy rates after transfer of the fresh and vitrified in vitro produced ovine blastocysts (P>0.05). The pregnancy rate of 2 embryos was higher than that of 1 embryo. The rate of embryo pregnancy decreased with the quality of embryo. The recipient pregnancy rate was not be affected by the blastocysts earlier than the synchronizing recipient on 24 hours after embryonic transfer (P>0.05). There was no significant difference in the receptor pregnancy rate between the grazing and feeding sheep embryos (P>0.05).【Conclusion】These results confirm that both embryonic and recipient factors play an important role in the success after embryo transfer in sheep.
    Comparative Study on Soybean Production in Kazakhstan and Xinjiang of China
    Sailihan Sai, WANG Zhi-min, JI Liang, Mukhtar Kudaibergenov, Azamat Khidirov, HU Bao-min, WANG Chang-hai, XUE Li-hua , CHEN Xing-wu, LU Jing, LEI Jun-jie, ZHANG Yong-qiang, WU Xin-yuan
    2017, 54(11): 2146-2156.  DOI: 10.6048/j.issn.1001-4330.2017.11.023
    Abstract ( )   PDF (2434KB) ( )  
    【Objective】 The soybean production, planting status and economic benefits in Kazakhstan were studied and compared with those in Xinjiang, China in the hope of exploring the potential of soybean production cooperation between Kazakhstan and Xinjiang, China.【Method】By means of field investigation and literature review, the soybean planting situation, existing varieties characteristics and planting distribution status in Kazakhstan were collected, sorted out and summarized. 【Result】Kazakhstan climate environment was similar to that of Xinjiang, China, which was favorable condition and foundation for planting soybean. In terms of production cost, planting management technology, germplasm resources development and utilization, land resources utilization and other fields, complementary advantages between the two sides were obvious.【Conclusion】Soybean production in Kazakhstan has such characteristics as low input cost, low yield and low price, so the cooperation in the fields of soybean planting, production, processing and trade between Xinjiang, China and Kazakhstan will have a broad promising prospect.