Isolation and identification of Poly (butylene adipate-co-terephthalate) -degrading Bacteria

doi:10.6048/j.issn.1001-4330.2017.11.016

Xinjiang Agricultural Sciences ›› 2017, Vol. 54 ›› Issue (11): 2086-2091.DOI: 10.6048/j.issn.1001-4330.2017.11.016

Isolation and identification of Poly (butylene adipate-co-terephthalate) -degrading Bacteria

HUO Xiang-dong¹, GAO Yan¹, LIN Qing¹, ZENG Jun¹, ZHANG Tao¹, CHU Min¹, YANG Hong-mei¹, SHI Ying-wu¹, WANG Bin², SUN Jiu-sheng², WANG Jin-xin²

1.Research Institute of Applied Microbiology/Xinjiang Special Environmental Microbiology Laboratory, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China;
2. Research Institute of Soil, Fertilizer and Agricultural Water Conservation, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China

Received:2017-09-30 Online:2017-11-20 Published:2017-12-06
Contact: Huo xiang-dong(1974-),male,Gansu province,associate professor,microbial resources,(E-mail)xiangdonghuo@163.com
Supported by:
The Key Research and Development Program of Xinjiang Uygur Autonomous Region "Biodegradable Plastic Film Innovation Project"(2016B02017-4); The National Natural Science Foundation of China"Metagenomic Library Construction of the Rumen Microbe from Xinjiang Bactrian Camel and cellulase Family Research" ( 31160027);The Special Funding for Enhancing the Agricultural Scientific and Technological Research Innovation Platform of Xinjiang Academy of Agricultural Sciences-Xinjiang Laboratory of Special Environmental Microbiology"(XJNKYPT-2017-002)

聚对苯二甲酸-己二酸丁二酯(PBAT)降解菌的分离鉴定和降解能力测定

霍向东¹, 高雁¹, 林青¹, 曾军¹, 张涛¹, 楚敏¹, 杨红梅¹, 史应武¹, 王斌², 孙九胜², 王金鑫²

1.新疆农业科学院微生物应用研究所/新疆特殊环境微生物实验室,乌鲁木齐 830091;
2.新疆农业科学院土壤肥料与农业节水研究所,乌鲁木齐 830091

作者简介:霍向东(1974-),男,甘肃人,副研究员,研究方向为微生物资源,(E-mail)xiangdonghuo@163.com
基金资助:
新疆维吾尔自治区重点研发项目“生物降解地膜创新工程”(2016B02017-4); 国家自然科学基金项目“新疆双峰驼瘤胃微生物宏基因组文库构建和纤维素酶系的研究”(31160027);“新疆农业科学院农业科技创新平台能力提升建设专项-新疆特殊环境微生物实验室”(XJNKYPT-2017-002)

Abstract

Abstract: 【Objective】 To isolate poly (butylene adipate-co-terephthalate)-degrading bacteria and study the capability of biodegradation of the bacteria.【Method】Using PBAT powder as the sole carbon source to isolate Poly (butylene adipate-co-terephthalate)-degrading bacteria from the soil which was covered with PBAT mulch. The strain was identified by 16S rDNA sequence comparative analysis. The degradation capability of strain in the liquid medium was evaluated by weight loss method and scanning electron microscope.【Result】1 Poly (butylene adipate-co-terephthalate) - degrading bacteria was isolated and identified as Sphingopyxis ginsengisoli . In the liquid medium, degradation rate of PBAT reached up to 0.92% after 60 days.【Conclusion】The Sphingopyxis ginsengisoli strain XJSL2 can be used to recycle of PBAT mulch. There are still a lot of soil microorganisms able to degrade PBAT, This still needs further research.

Key words: poly (butylene adipate-co-terephthalate); biodegradation; Sphingopyxis ginsengisoli

摘要： 【目的】研究能够降解聚对苯二甲酸-己二酸丁二酯(Poly(butylene adipate-co-terephthalate),PBAT)的微生物及其降解能力。【方法】以PBAT粉末为唯一碳源,从南疆疏勒县铺覆PBAT生物降解膜的土样中分离可有效降解PBAT聚合物的微生物,利用16S rDNA 序列对比分析进行菌株鉴定。采用失重法和扫描电镜观察,测定菌株的降解能力。【结果】从土壤中分离获得一株能够明显降解PBAT聚合物的细菌XJSL2,经16S rDNA 序列分析鉴定为Sphingopyxis ginsengisoli,经过60 d培养,该菌株对PBAT颗粒的实际降解率达到0.92%。【结论】菌株XJSL2能够显著降解PBAT聚合物,对于PBAT的再生利用具有潜在应用价值,土壤中还存在着大量能够降解PBAT的微生物。

关键词: 聚对苯二甲酸-己二酸丁二酯, 生物降解, Sphingopyxis ginsengisoli

CLC Number:

S188

HUO Xiang-dong, GAO Yan, LIN Qing, ZENG Jun, ZHANG Tao, CHU Min, YANG Hong-mei, SHI Ying-wu, WANG Bin, SUN Jiu-sheng, WANG Jin-xin. Isolation and identification of Poly (butylene adipate-co-terephthalate) -degrading Bacteria[J]. Xinjiang Agricultural Sciences, 2017, 54(11): 2086-2091.

霍向东, 高雁, 林青, 曾军, 张涛, 楚敏, 杨红梅, 史应武, 王斌, 孙九胜, 王金鑫. 聚对苯二甲酸-己二酸丁二酯(PBAT)降解菌的分离鉴定和降解能力测定[J]. 新疆农业科学, 2017, 54(11): 2086-2091.

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Isolation and identification of Poly (butylene adipate-co-terephthalate) -degrading Bacteria

聚对苯二甲酸-己二酸丁二酯(PBAT)降解菌的分离鉴定和降解能力测定

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