【Objective】 Effects of planting mode and irrigation quota on yield and fiber quality of machine-picked long-staple cotton and provide a theoretical basis for improving the quality of machine-picked long-staple cotton.【Methods】 This study adopted a split-plot experimental design.Under the same planting density, three planting patterns were set: one film with three rows (S3), one film with four rows (S4), and one film with six rows (S6).The three irrigation quotas were: 3,150 m3/hm2 [W1, severe deficit (field capacity 50%)], 4,050 m3/hm2 [W2, mild deficit (field capacity 75%)] and 4,980 m3/hm2 [ W3, slight deficit (field capacity 100%)].【Results】 Under different row spacing and irrigation quota, the accumulation of cotton dry matter conformed to the logistic growth function model.Under the same irrigation quota, with the expansion of the average row spacing, it enter the period of rapid dry matter accumulation earlier and increase Vm.The average Vm of S3 treatment 0.62 and 0.77 g/(plant·d) higher than that of S4 and S6 treatments, respectively.Under the same planting mode, with the increase of irrigation quota, the dry matter accumulation rate faster and the rapid accumulation period longer.The increase of average row spacing and irrigation quota significantly improved the number of bolls per plant and the quality of bolls, and finally increased the yield.There was no significant difference between W2 and W3 treatments.In terms of defoliation and branch hanging, under the same irrigation quota, with the expansion of the average row spacing, the defoliation rate of long-staple cotton was significantly increased, and the hanging branch rate was significantly reduced; under the same planting mode, with the increase of irrigation quota, oth leaf rate and branch hanging rate were significantly increased.In the interaction between the production and fiber quality, the defoliation rate of S3W3 treatment was the highest, and the branch hanging rate of S3W1 treatment was the lowest.In terms of fiber quality, the half-average length, uniformity and textile parameters increased with the increase of the average row spacing; the effect of irrigation quota on fiber quality was not significant, but the W1 treatment showed better fiber quality.【Conclusion】 The combination of equal row spacing and mild deficit irrigation (S3W2) is beneficial to the transfer of dry matter accumulation to reproductive organs, and the number of bolls per plant and the weight of single bolls are significantly increased, which is different from the seed cotton yield under the full irrigation treatment.And the defoliation rate is higher, the leaf hanging rate is lower, and the fiber quality is better.