Xinjiang Agricultural Sciences ›› 2023, Vol. 60 ›› Issue (5): 1041-1049.DOI: 10.6048/j.issn.1001-4330.2023.05.001

• Special volume for green, yield increasing, quality improving and eficiencyimproving technologies for major grain crops in Xinjiang • Previous Articles     Next Articles

Identification and evaluation of salt tolerance of rice recombinant inbred lines at bud and seedling stages

ZHANG Yanhong1(), HOU Tianyu1, BA Yinhua1, ZHAO Caiyue1, LYU Yuping2, Buhalikeimu Abunzi1, ZHAO Zhiqiang1, LI Dong2, DU Xiaojing2, YUAN Jie1, WANG Fengbin2()   

  1. 1. The State Key Laboratory of Genetic Improvement and Germplasm Innovation of Crop Resistance in Arid Desert Regions (Preparation)/Xinjiang Key Laboratory of Crop Biotechnology/Institute of Nuclear Technology and Biotechnology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China
    2. Key Laboratory of Oasis-Desert Crop Physiology Ecology and Cultivation of Agricultural Ministry of Agriculture and Rural Affairs/Institute of Food Crops, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China
  • Received:2022-09-05 Online:2023-05-20 Published:2023-05-22
  • Correspondence author: WANG Fengbin (1968-), male, born in Jiangxi Province, master degree, researcher, engaged in rice genetic breeding and cultivation technology, (E-mail)xjnkywfb@163.com
  • Supported by:
    Key R & D Program Project of Xinjiang Uygur Autonomous Region(2021B02002-3);Key S &T Project of Xinjiang Autonomous Region(2022A03004-3)

水稻重组自交系群体芽期和苗期耐盐性鉴定与评价

张燕红1(), 侯天钰1, 巴音花1, 赵彩月1, 吕玉平2, 布哈丽且木·阿不力孜1, 赵志强1, 李冬2, 杜孝敬2, 袁杰1, 王奉斌2()   

  1. 1.新疆农业科学院核技术生物技术研究所/干旱荒漠区作物抗逆遗传改良与种质创新国家重点实验室(筹)/新疆农作物生物技术重点实验室,乌鲁木齐 830091
    2.新疆农业科学院粮食作物研究所/农业农村部荒漠绿洲作物生理生态与耕作重点实验室,乌鲁木齐 830091
  • 通讯作者: 王奉斌(1968-),男,江西人,研究员,硕士,研究方向为水稻遗传育种及栽培,(E-mail)xjnkywfb@163.com
  • 作者简介:张燕红(1982-),女,甘肃武威人,副研究员,研究方向为水稻遗传育种,(E-mail)zhangyanhong9527@163.com
  • 基金资助:
    新疆维吾尔自治区重点研发项目(2021B02002-3);新疆维吾尔自治区重大科技专项(2022A03004-3)

Abstract:

【Objective】 Which seriously restricts the production and development of rice, so it is of great significance to identify the salt tolerance of rice germplasm resources at bud and seedling stages.The results have provided germplasm resources and reference for further digging salt-tolerant rice germplasm and breeding new varieties.【Methods】 A total of 219 stable recombinant inbred lines were used as experimental materials to identify and evaluate the salt tolerance of rice at bud stage and seedling stage using 1.2% NaCl solution and 0.7% NaCl solution, respectively.【Result】 The results showed that the germination potential and germination rate decreased significantly after the treatment with 1.2% NaCl, and 4 salt-tolerant materials and 6 sensitive materials were screened.The germination potential, germination rate, bud length and root length of salt-tolerant materials decreased slowly, while the germination potential, germination rate, bud length and root length of sensitive materials decreased significantly.After 0.7% NaCl treatment at seedling stage, the survival rate of population materials decreased significantly.The comparison of phenotypes between salt-tolerant materials and sensitive materials showed that the plant height and survival rate of salt-tolerant materials decreased but did not reach a significant level, while the plant height and survival rate of sensitive materials decreased significantly, and the inhibition degree of salt-tolerant materials was less than that of sensitive materials.【Conclusion】 The relative germination potential, relative germination rate and survival rate of 219 materials decreased significantly after salt stress at bud stage.The salinity tolerance of salt-tolerant materials is different from that of sensitive materials.The salinity stress of salt-tolerant materials is lower than that of sensitive materials.Eighteen materials with strong salt tolerance at budding stage are screened and seedlings of strong salt tolerance material 2 are also obtained.

Key words: rice; bud stage; seedling stage; salt tolerance; evaluation

摘要:

【目的】鉴定水稻种质资源芽期和苗期耐盐性,筛选耐盐性优良的种质资源,为挖掘水稻耐盐种质和培育耐盐新品种提供种质资源和参考依据。【方法】采用前期构建稳定的219份重组自交系为试验材料,在水稻的芽期和苗期分别利用1.2% NaCl和0.7% NaCl溶液鉴定和评价耐盐性。【结果】芽期用1.2% NaCl处理后发芽势和发芽率显著降低,筛选出4份耐盐材料和6份敏感材料。耐盐材料的发芽势、发芽率、芽长、根长下降缓慢,敏感材料发芽势、发芽率、芽长、根长显著下降。苗期用0.7% NaCl处理后,群体材料的存活率显著降低;耐盐材料和敏感材料的表型比较发现,耐盐材料株高和存活率有所下降但未达到显著水平,敏感材料株高和存活率显著下降,耐盐材料相比敏感材料受抑制程度较轻。【结论】219份材料的芽期受到盐胁迫后,相对发芽势、相对发芽率和存活率显著降低;耐盐材料和敏感材料的耐盐性不同,耐盐材料受盐胁迫的程度低于敏感材料;筛选出芽期耐盐性强材料18份;苗期耐盐性强材料2份。

关键词: 水稻, 芽期, 苗期, 耐盐性, 评价

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