Drought resistance evaluation of liaomian cotton varieties at germination stage under PEG simulated drought stress

doi:10.6048/j.issn.1001-4330.2023.11.007

Xinjiang Agricultural Sciences ›› 2023, Vol. 60 ›› Issue (11): 2653-2660.DOI: 10.6048/j.issn.1001-4330.2023.11.007

• Crop Genetics and Breeding · Germplasm Resources·Molecular Genetics·Soil Fertilizer • Previous Articles Next Articles

Drought resistance evaluation of liaomian cotton varieties at germination stage under PEG simulated drought stress

SHAN Ying¹(), LI Yue¹, XU Min¹, LIU Yanzhen¹, LI Ran², CHEN Jieyin², WANG Zisheng¹(), ZHU He¹()

1. Cash Crop Institute, Liaoning Academy of Agricultural Sciences, Liaoyang Liaoning 111000, China
2. State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received:2023-01-25 Online:2023-11-20 Published:2023-12-07
Correspondence author: ZHU He(1984-),male,associate research fellow,research direction: utilization and innovation of cotton germplasm resources,(E-mail)zhuhe19840204@163.com;WANG Zisheng(1965-),male,research fellow,doctor,research direction: cotton breeding, cultivation and promotion,(E-mail)wangzisheng6666@126.com
Supported by:
Liaohe Cotton Experiment Station, CARS(CARS-15-31);State Key Laboratory of Cotton Biology Open Fund(CB2022A25)

PEG模拟干旱胁迫下辽棉系列品种种子萌发期抗旱性评价

单莹¹(), 李越¹, 徐敏¹, 刘艳珍¹, 李冉², 陈捷胤², 王子胜¹(), 朱鹤¹()

1.辽宁省农业科学院经济作物研究所,辽宁辽阳 111000
2.中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室,北京 100193

通讯作者: 朱鹤(1984-),男,辽宁辽阳人,副研究员,研究方向为棉花种质资源,(E-mail)zhuhe19840204@163.com;王子胜(1965-),男,辽宁朝阳人,研究员,博士,研究方向为棉花育种栽培,(E-mail)wangzisheng6666@126.com
作者简介:单莹(1981-),女,辽宁辽阳人,助理研究员,硕士,研究方向为棉花遗传育种,(E-mail)shanying_sy@163.com
基金资助:
国家现代农业产业技术体系棉花产业技术体系辽河综合试验站(CARS-15-31);棉花生物学国家重点实验室开放课题基金(CB2022A25)

Abstract

Abstract:

【Objective】 To study the drought resistance at seed germination stage of six Liaomian cotton varieties under PEG simulated drought stress.【Methods】 The seeds of six Liaomian varieties were used as the testing materials to study drought resistance at seed germination stage under different concentrations of PEG-6000 (0, 2.5%, 5.0%, 7.5% and 10.0%). Germination energy, germination rate, germination index, germination drought resistance index, hypocotyl length, root length, seedling fresh weight and root fresh weight were measured under different drought stress.【Results】 The germination of cotton seeds was inhibited to different degrees, and the germination energy, germination rate and germination drought resistance index were decreased with the increase of PEG concentrations. Seedling growth index and root growth index were significantly inhibited under 2.5% PEG stress. 5.0% PEG stress promoted root length growth of all materials. The inhibition degrees of each index under PEG stress above 5.0% concentration were gradually increased with the increase of stress.【Conclusion】 The six Liaomian cotton varieties were sorted according to their drought resistance as follows: Liaomian 10>Liaomian 23>Liaomian 45>Liaomian 53>Liaomian 49>Liaomian 34.

Key words: cotton; PEG stress; germination stage; drought resistance

摘要：

【目的】 评价PEG模拟干旱胁迫下6个辽棉品种种子萌发期的抗旱性。【方法】 以6个辽棉系列审定品种为材料,采用不同浓度(0、2.5%、5.0%、7.5%、10.0%)的PEG-6000模拟干旱胁迫条件,设置萌发期抗旱试验,测定各材料对照和不同干旱胁迫下的发芽势、发芽率、萌发指数、萌发抗旱指数、下胚轴长、主根长、芽苗鲜重和根鲜重。【结果】 随PEG干旱胁迫程度的增加,棉花种子萌发均受到不同程度抑制,发芽势、发芽率、萌发抗旱指数随胁迫程度的加重而降低,幼苗生长指标和根系生长指标在2.5% PEG胁迫受抑制显著,5.0% PEG胁迫促进了各材料的根长生长,各指标在5.0% 浓度以上PEG胁迫时受抑制程度随胁迫增加逐渐增强。【结论】 6个辽棉系列品种的抗旱性由强至弱的顺序为辽棉10号>辽棉23号>辽棉45号>辽棉53号>辽棉49号>辽棉34号。

关键词: 棉花, PEG胁迫, 萌发期, 抗旱性

CLC Number:

S562

SHAN Ying, LI Yue, XU Min, LIU Yanzhen, LI Ran, CHEN Jieyin, WANG Zisheng, ZHU He. Drought resistance evaluation of liaomian cotton varieties at germination stage under PEG simulated drought stress[J]. Xinjiang Agricultural Sciences, 2023, 60(11): 2653-2660.

单莹, 李越, 徐敏, 刘艳珍, 李冉, 陈捷胤, 王子胜, 朱鹤. PEG模拟干旱胁迫下辽棉系列品种种子萌发期抗旱性评价[J]. 新疆农业科学, 2023, 60(11): 2653-2660.

Figures/Tables 10

References 18

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浓度 Concentrations (%)	品种Varieties
浓度 Concentrations (%)	辽棉10号 Liaomian 10	辽棉23号 Liaomian 23	辽棉34号 Liaomian 34	辽棉45号 Liaomian 45	辽棉49号 Liaomian 49	辽棉53号 Liaomian 53
CK	92.0±2.0^a	95.3±2.3^a	88.0±2.0^a	95.3±3.1^a	96.0±3.5^a	96.0±4.0^a
2.5	85.3±2.3^b	90.0±4.0^ab	82.0±3.5^b	90.7±3.1^a	81.3±4.6^b	75.3±5.0^b
5.0	66.7±3.1^c	75.3±1.2^c	48.0±2.0^c	81.3±2.3^b	70.0±3.5^c	54.7±5.0^c
7.5	65.3±3.1^c	84.7±4.2^b	38.0±2.0^d	78.7±3.1^b	23.3±3.1^d	50.7±1.2^c
10.0	50.0±3.5^d	56.7±4.2^d	26.0±2.0^e	42.0±4.0^c	7.3±2.3^e	28.7±3.1^d

浓度 Concentrations (%)	品种Varieties
浓度 Concentrations (%)	辽棉10号 Liaomian 10	辽棉23号 Liaomian 23	辽棉34号 Liaomian 34	辽棉45号 Liaomian 45	辽棉49号 Liaomian 49	辽棉53号 Liaomian 53
CK	92.0±2.0^a	95.3±2.3^a	88.0±2.0^a	95.3±3.1^a	96.0±3.5^a	96.0±4.0^a
2.5	85.3±2.3^b	90.0±4.0^ab	82.0±3.5^b	90.7±3.1^a	81.3±4.6^b	75.3±5.0^b
5.0	66.7±3.1^c	75.3±1.2^c	48.0±2.0^c	81.3±2.3^b	70.0±3.5^c	54.7±5.0^c
7.5	65.3±3.1^c	84.7±4.2^b	38.0±2.0^d	78.7±3.1^b	23.3±3.1^d	50.7±1.2^c
10.0	50.0±3.5^d	56.7±4.2^d	26.0±2.0^e	42.0±4.0^c	7.3±2.3^e	28.7±3.1^d

品种Varieties	R²	回归方程Regression equation	Y=80,X值 Y=80,X value
辽棉10号Liaomian 10	0.953 3	Y=92.667-4.16X	3.04
辽棉23号Liaomian 23	0.740 9	Y=96.933-3.306 7X	5.12
辽棉34号Liaomian 34	0.944 8	Y=90-6.72X	1.49
辽棉45号Liaomian 45	0.796 6	Y=101.33-4.746 7X	4.49
辽棉49号Liaomian 49	0.943 1	Y=102.67-9.413 3X	2.41
辽棉53号Liaomian 53	0.968	Y=92.933-6.373 3X	2.03

品种Varieties	R²	回归方程Regression equation	Y=80,X值 Y=80,X value
辽棉10号Liaomian 10	0.953 3	Y=92.667-4.16X	3.04
辽棉23号Liaomian 23	0.740 9	Y=96.933-3.306 7X	5.12
辽棉34号Liaomian 34	0.944 8	Y=90-6.72X	1.49
辽棉45号Liaomian 45	0.796 6	Y=101.33-4.746 7X	4.49
辽棉49号Liaomian 49	0.943 1	Y=102.67-9.413 3X	2.41
辽棉53号Liaomian 53	0.968	Y=92.933-6.373 3X	2.03

浓度 Concentrations (%)	品种Varieties
浓度 Concentrations (%)	辽棉10号 Liaomian 10	辽棉23号 Liaomian 23	辽棉34号 Liaomian 34	辽棉45号 Liaomian 45	辽棉49号 Liaomian 49	辽棉53号 Liaomian 53
CK	92.7±1.2^a	98.0±2.0^a	94.0±2.0^a	97.3±3.1^a	98.7±1.2^a	97.3±2.3^a
2.5	89.3±4.2^ab	94.7±3.1^ab	85.3±2.3^b	94.0±2.0^ab	92.0±2.0^b	80.0±2.0^b
5.0	88.7±4.2^ab	93.3±3.1^ab	81.3±3.1^b	88.0±3.5^c	87.3±3.1^b	80.0±4.0^b
7.5	83.3±3.1^b	91.3±5.0^b	62.7±3.1^c	88.7±3.1^bc	68.7±4.6^c	56.0±3.5^c
10.0	72.7±3.1^c	80.0±2.0^c	60.7±3.1^c	64.7±3.1^d	28.0±2.0^c	54.7±4.2^c

Drought resistance evaluation of liaomian cotton varieties at germination stage under PEG simulated drought stress

PEG模拟干旱胁迫下辽棉系列品种种子萌发期抗旱性评价

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品种Varieties	R²	回归方程 Regression equation	Y=80,X值 Y=80,X value
辽棉10号Liaomian 10	0.862 5	Y=94.533-1.84X	7.90
辽棉23号Liaomian 23	0.823 3	Y=99.333-1.573 3X	12.29
辽棉34号Liaomian 34	0.939 7	Y=94.667-3.573 3X	4.10
辽棉45号Liaomian 45	0.759 8	Y=100.67-2.826 7X	7.31
辽棉49号Liaomian 49	0.834 2	Y=107.87-6.586 7X	4.23
辽棉53号Liaomian 53	0.910 1	Y=95.467-4.373 3X	3.54

品种 Varieties	隶属函数值 Subordinate function value							平均值 Average	抗旱排名 Sort
品种 Varieties	相对发芽势 RGE	相对发芽率 RGR	相对下胚轴长 RHL	相对根长 RRL	相对苗鲜重 RSFW	相对根鲜重 RRFW	萌发抗旱指数 GDRI	平均值 Average	抗旱排名 Sort
辽棉10号 Liaomian 10	0.77	0.96	0.67	0.51	0.72	0.80	0.57	0.715	1
辽棉23号 Liaomian 23	0.94	0.99	0.30	0.97	0.24	0.54	0.97	0.707	2
辽棉34号 Liaomian 34	0.40	0.46	0.53	0.25	0.28	0.38	0.20	0.356	6
辽棉45号 Liaomian 45	0.90	0.82	0.20	0.76	0.40	0.59	0.89	0.652	3
辽棉49号 Liaomian 49	0.24	0.39	0.22	0.38	0.56	0.59	0.21	0.373	5
辽棉53号 Liaomian 53	0.24	0.13	0.74	0.66	0.70	0.25	0.16	0.411	4

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浓度 Concentrations (%)	品种Varieties
浓度 Concentrations (%)	辽棉10号 Liaomian 10	辽棉23号 Liaomian 23	辽棉34号 Liaomian 34	辽棉45号 Liaomian 45	辽棉49号 Liaomian 49	辽棉53号 Liaomian 53
2.5	0.88^a	0.93^a	0.83^a	0.94^a	0.84^a	0.79^a
5.0	0.72^b	0.84^b	0.58^b	0.85^ab	0.72^b	0.58^b
7.5	0.66^b	0.84^b	0.43^c	0.82^b	0.40^c	0.53^b
10.0	0.43^c	0.67^c	0.38^c	0.46^c	0.13^d	0.32^c

浓度 Concentrations (%)	品种Varieties
浓度 Concentrations (%)	辽棉10号 Liaomian 10	辽棉23号 Liaomian 23	辽棉34号 Liaomian 34	辽棉45号 Liaomian 45	辽棉49号 Liaomian 49	辽棉53号 Liaomian 53
2.5	0.88^a	0.93^a	0.83^a	0.94^a	0.84^a	0.79^a
5.0	0.72^b	0.84^b	0.58^b	0.85^ab	0.72^b	0.58^b
7.5	0.66^b	0.84^b	0.43^c	0.82^b	0.40^c	0.53^b
10.0	0.43^c	0.67^c	0.38^c	0.46^c	0.13^d	0.32^c