棉花与几种作物根际土壤酶活性及细菌多样性分析

doi:10.6048/j.issn.1001-4330.2020.08.016

新疆农业科学 ›› 2020, Vol. 57 ›› Issue (8): 1514-1526.DOI: 10.6048/j.issn.1001-4330.2020.08.016

• 植物保护·微生物·土壤肥料·农产品分析检测·草业 • 上一篇下一篇

棉花与几种作物根际土壤酶活性及细菌多样性分析

刘海洋, 王伟, 张仁福, 姚举

新疆农业科学院植物保护研究所/农业部西北荒漠绿洲作物有害生物综合治理重点实验室,乌鲁木齐 830091

收稿日期:2019-10-16 发布日期:2020-09-01
通信作者: 姚举(1969-),男,山东人,研究员,研究方向为棉花病虫害综合防控,(E-mail)yaoju500@sohu.com
作者简介:刘海洋(1982-),男,山东人,副研究员,博士,研究方向为棉花病害综合防控,(E-mail)liuhaiyang001@163.com
基金资助:
自治区公益性科研院所基本科研业务费(KY201807);新疆维吾尔自治区科技支疆项目(2019E0244);国家重点研发计划(2017YFD0201903,2017YFD0201106)

Analysis of Soil Enzyme Activity and Bacterial Diversity in Rhizosphere of Cotton and Several Economic Crops

LIU Haiyang, WANG Wei, ZHANG Renfu, YAO Ju

Key Laboratory of Intergraded Crop Pest Management in China North-Western Oasis, Ministry of Agriculture and Rural Affairs, P. R. China / Research Institute of Plant Protection, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China

Received:2019-10-16 Published:2020-09-01
Correspondence author: YAO Ju (1969- ), male, Shandong, Professor, research field: study on integrated prevention and control of cotton diseases and pests,(E-mail)yaoju500@sohu.com
Supported by:
Supported by the Basic Scientific R & D Program of Public Welfare Research Institutions of Xinjiang Uygur Autonomous Region of China (KY201807); S&T Supporting Xinjiang Project (2019E0244); the National Key R & D Program Project of China (2017YFD0201903 and 2017YFD0201106)

摘要/Abstract

摘要： 【目的】 研究种植不同抗病性棉花以及不同经济作物对根际土壤酶活性以及细菌多样性影响,为棉田土壤微生态调控技术提供依据。【方法】 利用土壤酶检测试剂盒及高通量测序技术,检测分析不同抗病性棉花及种作物根际土壤酶活性及细菌多样性。【结果】 不同抗病性棉花根际土壤过氧化氢酶、脱氢酶、脲酶活性均没有显著差异,5与7月2个采样时期之间无显著差异;棉花与水稻间作处理过氧化氢酶、脱氢酶和脲酶分别较棉花单作升高3.84%、28.7%、45.2%,水稻处理较棉花单作升高8.87%、102.4%、80.4%,间作对土壤酶活性表现出提升作用,但未达到显著水平;苜蓿、大豆、花生3种豆科作物根际土壤酶活性普遍高于甜菜、番茄和玉米,但是未达到显著差异。耐病品种新陆中66号与感病品种军棉1号根际土壤的细菌OTU数量和α多样性指数均无显著差异。大豆、花生、苜蓿3种豆科作物根际土壤细菌的OTU数量和Ace、Chao1指数均高于棉花,番茄、玉米、甜菜3种作物根际土壤细菌OTU数量和α多样性指数均低于棉花,其中大豆的增幅较大,甜菜降幅较大,但是不同作物之间未达到显著性差异。【结论】 单季生育期内,种植不同抗病性棉花品种和不同作物、棉花-水稻间作等均未对根际土壤酶活性、细菌数量和多样性造成显著影响,棉花与不同作物搭配、间作等模式改变棉田土壤的微生态环境可能需要较长的生长周期。

关键词: 根际土壤; 土壤酶活性; 细菌多样性; 间作

Abstract: 【Objective】 The objective of the present investigation is to clarify effects of planting different disease-resistant cottons and different economic crops on soil enzyme activity and bacterial diversity in rhizosphere, and provide basis for exploring soil microecological regulation and control techniques in cotton fields.【Method】 Soil enzyme activity and bacterial diversity in rhizosphere of different disease-resistant cottons and six kinds of economic crops were detected by employing soil enzyme detection kit and high throughput sequencing technology.【Result】 There was no significant difference in catalase, dehydrogenase and urease activities in rhizosphere soil of cotton with different disease resistance, and there was no significant difference of the two sampling periods between May and July. Catalase, dehydrogenase and urease in the intercropping treatment between cotton and rice (Oryza sativa L.) were 3.84%, 28.7% and 45.2% higher than those in cotton monoculture, respectively, and those in rice treatment were 8.87%, 102.4% and 80.4% higher than those in cotton monoculture, respectively. Intercropping had an increase effect on soil enzyme activity, but it did not reach a significant level. The activities of soil enzymes in rhizosphere of three leguminous crops, Medicago sativa L., soybean and peanut, were generally higher than those of sugar beet, tomato and corn, but there was no significant difference. There was no significant difference in bacterial operational taxonomic unit (OTU) number and α diversity index between disease-resistant variety Xinluzhong 66 and disease-susceptible variety Junmian 1. The OTU number and Ace and Chao1 index of soil bacteria in rhizosphere of soybean, peanut and Medicago sativa L. were higher than those of cotton. The OTU number and α diversity index of soil bacteria in rhizosphere of three crops of tomato, corn and beet were lower than those of cotton, of which the increase of those of soybean was greater while the decrease of those of beet was greater, but there was no significant difference among different crops.【Conclusion】 During the single growth period, planting different disease-resistant cotton varieties and different crops, cotton-rice intercropping did not significant affect the enzyme activity, bacterial number and diversity of rhizosphere soil. It may take a longer growth cycle to change the soil microecological environment of cotton field through cotton and different crop collocation and intercropping and other modes.

Key words: rhizosphere soil; soil enzyme activity; bacterial diversity; intercropping

中图分类号:

S562
S188

刘海洋, 王伟, 张仁福, 姚举. 棉花与几种作物根际土壤酶活性及细菌多样性分析[J]. 新疆农业科学, 2020, 57(8): 1514-1526.

LIU Haiyang, WANG Wei, ZHANG Renfu, YAO Ju. Analysis of Soil Enzyme Activity and Bacterial Diversity in Rhizosphere of Cotton and Several Economic Crops[J]. Xinjiang Agricultural Sciences, 2020, 57(8): 1514-1526.

参考文献

[1] Allison V, Condron L, Peltzer D A, et al. Changes in enzyme activities and soil microbial community composition along carbon and nutrient gradients at the Franz Josef chronos-equence, New Zealand [J]. Soil Biology and Biochemistry, 2007, 39(7):1770-1781.
[2] Acostamart E V, Zobeck T M, Gill T E, et al. Enzyme activities and microbial community structure in semiarid agricultural soils [J]. Biology and Fertility of Soils, 2003, 38(4):216-227.
[3] Petra F, Katja K, Katrin K, et al. Soil enzyme activities as bioindicators for substrate quality in revegetation of a subtropical coal mining dump[J]. Soil Biology and Biochemistry, 2013, 56:87-89.
[4] 娄翼来, 关连珠, 王玲莉, 等. 不同植烟年限土壤pH和酶活性的变化[J]. 植物营养与肥料学报, 2007, 13 (3): 531-534.
LOU Yilai, GUAN Lianzhu, WANG Lingli, et al. Changes of pH and enzyme activities in soils fordifferent tobacco cropping years[J]. Plant Nutrition and Fertilizer Science, 2017, 13 (3): 531-534.
[5] 吕家珑, 张一平. 石灰性土壤小麦根际pH及磷动态变化的研究[J]. 植物营养与肥料学报, 1999, 5 (1): 32-39.
LÜ Jialong, ZHANG Yiping. Dynamic of pH and phosphorus of wheat rhizosphere in calcareous soil [J]. Plant Nutrition and Fertilizer Science, 1999, 5(1): 32-39.
[6] 王理德, 王方琳, 郭春秀, 等. 土壤酶学硏究进展[J]. 土壤, 2016, 48 (1): 12-21.
WANG Lide, WANG Fanglin, GUO Chunxiu, et al. Review: progress of soil enzymology[J]. Soils, 2016,48 (1): 12-21.
[7] 张向前, 黄国勤, 卞新民, 等. 间作对玉米品质、产量及土壤微生物数量和酶活性的影响[J]. 生态学报, 2012, 32 (22): 7082-7090.
ZHANG Xiangqian, HUANG Guoqin, BIAN Xinmin, et al. Effects of intercropping on quality and yield of maize grain, microorganism quantity, and enzyme activities in soils [J]. Acta Ecologica Sinica, 2012, 32 (22): 7082-7090.
[8] 贾志红, 易建华, 苏以荣, 等. 烟区轮作与连作土壤细菌群落多样性比较[J]. 生态环境学报, 2010, 19 (7): 1578-1585.
JIA Zhihong, YI Jianhua , SU Yirong, et al. Diversity comparison of soil bacteria communities in rotation and continuous flue-cured tobacco cropping[J]. Ecology and Environmental Science, 2010, 19 (7):1578-1585.
[9] 谷岩, 邱强, 王振民,等. 连作大豆根际微生物群落结构及土壤酶活性[J]. 中国农业科学, 2012, 45(19): 3955–3964.
GU Yan, QIU Qiang, WANG Zhenmin, et al. Effects of soybean continuous cropping on microbial and soil enzymes in soybean rhizo-sphere [J]. Scientia Agricultura Sinica, 2012, 45 (19): 3955–3964.
[10] 王志刚, 徐伟慧, 郭天文. 韭菜连作对大棚土壤微生物和酶活性的影响[J]. 土壤通报, 2010, 41 (5): 1048–1052.
WANG Zhigang, XU Weihui, GUO Tianwen. Effects of Chinese chives’ continuous cropping on microbial quantity and enzymes activities in the soil of big cote [J]. Chinese Journal of Soil Science, 2010, 41(5): 1048–1052.
[11] Sun C X, Chen L J, Wu Z J, et al. Soil persistence of Bacillus thuringiensis (Bt) toxin from transgenic Bt cotton tissues and its effect on soil enzyme activities[J]. Biology and Fertility of Soils, 2007, 43 (5):617-620.
[12] 刘海洋, 王琦, 王伟, 等. 新疆棉花黄萎病的发生现状及其病原菌的分子鉴定与ISSR分析[J]. 植物保护学报, 2018, 45(6):1194-1203.
LIU Haiyang, WANG Qi, WANG Wei, et al. Molecular identification and ISSR analysis of Verticillium dahliae and the current status of cotton Verticillium wilt in Xinjiang[J]. Journal of Plant Protection, 2018, 45(6):1194-1203.
[13] 李鹏程, 董合林,刘敬然, 等. 棉田土壤酶活性对尿素用量的响应[J]. 棉花学报,2015,27(6):542-549.
LI Pengcheng, DONG Helin, LIU Jingran, et al. Response of enzyme activity of soil to urea application rate in a cotton field trial [J]. Cotton Science, 2015, 27 (6):542-549.
[14] 陶磊, 褚贵新, 刘涛, 等. 有机肥替代部分化肥对长期连作棉田产量、土壤微生物数量及酶活性的影响[J]. 生态学报, 2014, 34(21): 6137-6146.
TAO Lei, CHU Guixin, LIU Tao, et al. Impacts of organic manure partial substitution for chemical fertilizer on cotton yield, soil microbial community and enzyme activities in mono-cropping system in drip irrigation condition[J]. Acta Ecologica Sinica, 2014, 34(21): 6137-6146.
[15] 田小明, 李俊华, 王成, 等. 连续3年施用生物有机肥对土壤养分、微生物生物量及酶活性的影响[J]. 土壤, 2014, 46(3): 481-488.
TIAN Xiaoming, LI Junhua, WANG Cheng, et al. Effects of continuous application of bio-organic fertilizer for three years on soil nutrients, microbial biomass and enzyme activity [J]. Soils, 2014, 46(3): 481-488.
[16] 刘艳慧, 王双磊, 李金埔, 等. 棉花秸秆还田对土壤微生物数量及酶活性的影响[J]. 华北农学报, 2016, 31(6):151-156.
LIU Yanhui, WANG Shuanglei, LI Jin-pu, et al. Effects of cotton straw returning soil on soil microbes quantites and enzyme activities [J]. Acta Agriculturae Boreali-Sinica, 2016, 31(6):151-156.
[17] 孟自力, 叶美金, 闫延梅, 等. 间作大蒜对小麦根际土壤微生物数量及土壤酶活性的影响[J]. 农业资源与环境学报, 2018, 35(5):430-438.
MENG Zili, YE Meijin, YAN Yanmei, et al. Soil microorganism quantity and soil enzyme activity in the wheat-garlic intercropping system[J]. Journal of Agricultural Resources and Environment, 2018, 35(5):430-438.
[18] 蔡伟, 王焱, 伏兵哲, 等.宁夏引黄灌区苜蓿品种对土壤微生物数量及酶活性的影响[J].基因组学与应用生物学, 2018, 37(10):4349-4356.
CAI Wei, WANG Yan, FU Bingzhe, et al. Effects of alfalfa cultivars on amount of soil microorganisms and enzyme activity in Ningxia yellow river irrigation area [J]. Genomics and Applied Biology, 2018, 37(10):4349-4356.
[19] 王晓军, 于凤芝, 宿庆瑞, 等. 不同绿肥品种综合利用价值的比较[J]. 黑龙江农业科学, 2010, (6): 55–57.
WANG Xiaojun, YU Fengzhi, SU Qingrui, et al. Comparison of comprehensive utilization value with different green manure varieties [J]. Heilongjiang Agricultural Sciences, 2010, (6): 55–57.
[20] 刘丽青, 仪慧兰, 郭二虎, 等. 不同谷子品种黑穗病抗性及土壤酶活性分析[J]. 山西大学学报(自然科学版), 2016, 39(1):152-157.
LIU Liqing, YI Huilan, GUO Erhu, et al.Analysis of ustilago crameri resistance and soil enzyme activity of different millet varieties [J]. Journal of Shanxi University (Natural Science Edition), 2016,39(1):152-157.
[21] Coleman D C , Hunter M D , Hutton J , et al. Soil respiration from four aggrading forested watersheds measured over a quarter century [J]. Forest Ecology and Management, 2002, 157(1-3):247-253.
[22] Monreal C M, Bergstromd W. Soil enzymatic factors expressing the influence of land use, tillage system an d texture on soil biochemical quality [J]. Canadian Journal of Soil Science, 2000, 80:419-428.
[23] 刘瑜, 梁永超, 褚贵新, 等. 长期棉花连作对北疆棉区土壤生物活性与酶学性状的影响[J]. 生态环境学报 2010, 19 (7): 1586-1592.
LIU Yu, LIANG Yongchao, CHU Guixin, et al. Effects of long-term cotton monocropping on soilbiological characteristics and enzyme activities in Northern Xinjiang[J]. Ecology and Environment, 2010,19 (7): 1586-1592.
[24] 张旭龙, 马森, 吴振振, 等. 油葵与光果甘草间作对根际土壤酶活性及微生物功能多样性的影响[J]. 土壤, 2016, 48 (6):1114-1119.
ZHANG Xulong, MA Sen, WU Zhenzhen, et al. Effects of helianthus annuus and glycyrrhiza glabra intercropping on rhizosphere soil enzyme activities and soil microbes functional diversity [J]. Soils, 2016, 48 (6):1114-1119.
[25] 孟亚利, 王立国, 周治国, 等. 套作棉根际与非根际土壤酶活性和养分的变化[J]. 应用生态学报, 2005, 16(11): 2076-2080.
MENG Yali, WANG Liguo, ZHOU Zhiguo, et al. Dynamics of soil enzyme activity and nutrientcontent in intercropped cotton rhizosphere and non-rhizosphere[J]. Journal of Applied Ecology, 2005, 16(11): 2076-2080.
[26] 李伶俐, 黄耿华, 李彦鹏, 等. 棉花与不同作物同穴互作育苗对土壤微生物、酶活性和根系分泌物的影响[J]. 植物营养与肥料学报, 2012, 18 (6):1475-1482.
LI Lingli, HUANG Genghua, LI Yanpeng, et al. Effects of mutual aid grow seedlings of cotton with other crops in the same aperture on soil microorganisms quantity, enzyme activity and root secretion [J]. Plant Nutrition and Fertilizer Science, 2012, 18 (6):1475-1482.
[27] 谢利, 王燕芳, 马超, 等. 棉花-孜然间作模式对土壤微生物数量及酶活性的影响[J]. 江苏农业科学, 2015, 43 (10):103-105.
XIE Li, WANG Yanfang, MA Chao, et al. Effects of cotton and cumin intercropping on soil microbial population and enzyme activities [J]. Jiangsu Agricultural Sciences, 2015, 43 (10):103-105.
[28] 张明发, 田峰, 王兴祥, 等. 翻压不同绿肥品种对植烟土壤肥力及酶活性的影响[J].土壤, 2017, 49 (5): 903–908.
ZHANG Mingfa, TIAN Feng, WANG Xingxiang, et al. Effects of different green manure varieties on enzyme activities and fertility of tobacco-planting soils [J]. Soils, 2017, 49 (5): 903–908.
[29] 李红燕, 胡铁成, 曹群虎, 等. 旱地不同绿肥品种和种植方式提高土壤肥力的效果[J]. 植物营养与肥料学报, 2016, 22 (5): 1310–1318.
LI Hongyan, HU Tiecheng, CAO Qunhu, et al. Effect of improving soil fertility by planting different green manures in different patterns in dryland [J]. Plant Nutrition and Fertilizer Science, 2016, 22 (5): 1310–1318.
[30] 顾美英, 徐万里, 茆军, 等. 连作对新疆绿洲棉田土壤微生物数量及酶活性的影响[J]. 干旱地区农业研究, 2009, 27 (1):1-5.
GU Meiying, XU Wani, MAO Jun, et al.Effects of cotton continuous cropping on the amount of soil microbes and enzyme activities in Xinjiang [J]. Agricultural Research in the Arid Areas, 2009, 27 (1):1-5.

棉花与几种作物根际土壤酶活性及细菌多样性分析

Analysis of Soil Enzyme Activity and Bacterial Diversity in Rhizosphere of Cotton and Several Economic Crops

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