柽柳根际土壤放线菌的分离及拮抗活性筛选

doi:10.6048/j.issn.1001-4330.2023.07.027

新疆农业科学 ›› 2023, Vol. 60 ›› Issue (7): 1790-1797.DOI: 10.6048/j.issn.1001-4330.2023.07.027

柽柳根际土壤放线菌的分离及拮抗活性筛选

陈乙煌¹(), 邢利¹, 东珍珍¹, 马小梅¹, 黄建军², 罗晓霞¹()

1.塔里木大学生命科学学院,新疆阿拉尔 843300
2.新疆阿拉尔市十三团农业发展服务中心,新疆阿拉尔 843300

收稿日期:2022-10-30 出版日期:2023-07-20 发布日期:2023-07-11
通信作者: 罗晓霞(1982-),女,重庆人,硕士生导师,研究方向为微生物次级代谢产物挖掘,(E-mail)xxluo415@163.com
作者简介:陈乙煌(1998-),男,福建泉州人,研究生,研究方向为微生物次级代谢产物挖掘,(E-mail)375093055@qq.com
基金资助:
新疆生产建设兵团中青年领军人才项目(2019CB030);区域创新引导计划(2021BB007)

Isolation and antagonistic activity screening of Actinomycetes from rhizosphere soil of Tamarix

CHEN Yihuang¹(), XING Li¹, DONG Zhenzhen¹, MA Xiaomei¹, HUANG Jianjun², LUO Xiaoxia¹()

1. College of Life Sciences, Tarim University, Aral Xinjiang 843300, China
2. Aral Municipal Agricultural Development Service Center of 13th Agricultural Regiment, Aral Xinjiang 843300, China

Received:2022-10-30 Online:2023-07-20 Published:2023-07-11
Correspondence author: LUO Xiaoxia(1982-), female, master supervisor, research direction:Mining of microbial secondary metabolites,(E-mail)xxluo415@163.com
Supported by:
Young and Middle-aged Leading Talents Project(2019CB030);Regional Innovation Guidance Plan(2021BB007)

摘要/Abstract

摘要：

【目的】分析柽柳根际土壤放线菌物种多样性,研究其抗菌活性,为新疆主要农牧业病害的防控奠定菌种基础。【方法】选择新疆南疆地区10种柽柳根际土壤的放线菌资源,采用四种培养基,通过菌株16SrDNA测序,采用平板对峙法筛选抗菌活性。【结果】分离出294株菌株,鉴定的150株,分属于7个纲12个目16科24个属,另有9个潜在新种,以金黄色葡萄球菌、铜绿假单胞菌、大肠杆菌、肺炎克雷伯菌、解淀粉欧文氏菌、伤寒沙门氏菌、白色念珠菌、链格孢菌、棉花黄萎病菌、棉花枯萎病菌为靶标菌。【结论】筛选出68株活性放线菌。柽柳根际土壤放线菌的分离丰富了微生物资源及菌种的储备。

关键词: 柽柳根际土壤; 放线菌; 物种多样性

Abstract:

【Objective】 To lay a foundation for the prevention and control of major agricultural and animal husbandry diseases in Xinjiang.【Methods】 Ten species of actinomycetes from rhizosphere soils of Tamarix ramosissima in southern Xinjiang were selected and their antibactieral activities were studied.Species diversity of actinomycetes in rhizosphere soil of Tamarix ramosissima was analyzed by 16SrDNA sequencing using four kinds of media.The antibacterial activity was screened by plate confrontation methods.The antibacterial activity was screened by plate confrontation method.【Results】 The results showed that total 294 strains were isolated, and total 150 strains were identified, which belonged to seven classes and twelve orders, total 12 families, total 24 genera, and 9 potential new species.At the same time, the antibacterial activity was analyzed with Staphylococcus aureus, Pseudonas aeruginosa, Escherichia coli, Klebsiella pneumonia, Erwinia amylohydrolytic, Slmonella typhoid, Candida albicans, Chain bacteria Verticilliuma dhiiae, Fusarium oxysporum as the target bacteria.【Conclusion】 Total 68 strains of active actinmycetes were screened by antibacterial activity analysis. Isolation of actinomycetes from rhizosphere soil of Tamarix ramosissima enriched microbial resources and species reserve.

Key words: rhizosphere soil of Tamarix; actinomycetes; species diversity

中图分类号:

陈乙煌, 邢利, 东珍珍, 马小梅, 黄建军, 罗晓霞. 柽柳根际土壤放线菌的分离及拮抗活性筛选[J]. 新疆农业科学, 2023, 60(7): 1790-1797.

CHEN Yihuang, XING Li, DONG Zhenzhen, MA Xiaomei, HUANG Jianjun, LUO Xiaoxia. Isolation and antagonistic activity screening of Actinomycetes from rhizosphere soil of Tamarix[J]. Xinjiang Agricultural Sciences, 2023, 60(7): 1790-1797.

图/表 5

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土样编号 Soil sample number	样品类型 Soil sample type	取样位置 Sampling location	纬度 Latitude (°)	经度 Longitude (°)	海拔 Altitude (m)
HT44	红柳根际土	塔中-民丰	38.412	83.205	1 164.9
HT51	红柳根际土	萨勒吾则克乡	37.254	83.018	1 386.6
HT53	红柳根际土	民丰-且末	37.688	83.959	1 341.1
HT57	红柳根际土	塔提让乡	38.485	85.824	1 112.1
HT61	红柳根际土	瓦石峡乡	38.819	87.743	885.2
HT65	红柳根际土	若羌-库尔勒	39.889	88.399	814.3
HT67	红柳根际土	大西海子水库	40.562	87.810	829.6
HT71	红柳根际土	沙雅	41.137	82.446	957.8
HT75	红柳根际土	二牧场-14团	40.660	81.943	975.1
HT49	红柳根际土	民丰-于田	36.888	82.553	1 664.8

土样编号 Soil sample number	样品类型 Soil sample type	取样位置 Sampling location	纬度 Latitude (°)	经度 Longitude (°)	海拔 Altitude (m)
HT44	红柳根际土	塔中-民丰	38.412	83.205	1 164.9
HT51	红柳根际土	萨勒吾则克乡	37.254	83.018	1 386.6
HT53	红柳根际土	民丰-且末	37.688	83.959	1 341.1
HT57	红柳根际土	塔提让乡	38.485	85.824	1 112.1
HT61	红柳根际土	瓦石峡乡	38.819	87.743	885.2
HT65	红柳根际土	若羌-库尔勒	39.889	88.399	814.3
HT67	红柳根际土	大西海子水库	40.562	87.810	829.6
HT71	红柳根际土	沙雅	41.137	82.446	957.8
HT75	红柳根际土	二牧场-14团	40.660	81.943	975.1
HT49	红柳根际土	民丰-于田	36.888	82.553	1 664.8

序号	菌株编号	相似菌株	相似性(%)	抑菌圈直径类型(mm)
序号	菌株编号	相似菌株	相似性(%)	S.a	P.a	E.c	K.p	E.a	S.t	C.a	A.t	V.d	F.o
1	TRM 76131	Cellulosimicrobium marinum RS-7-4	98.99	—	—	+	—	+	+	++	—	+++	+
2	TRM 76130	Rhizobium tarimense PL-41	99.71	++	—	+	—	—	+	+++		+++	++
3	TRM 76129	Salmonella enterica subsp.entericaL T2	99.23	—	—	—	+	+	+	++	++	++	—
4	TRM 76146	Rhizobium tarimense PL-41	99.05	—	—	+	—	+	+	—	+	+	—
5	TRM 76147	Microbacterium petrolearium LAM0410	98.56	—	+	—	—	—	—	+	+++	+++	+
6	TRM 76156	Rhizobium tarimense PL-41	98.84	—	—	—	—	—	—	+++	+++	+++	+
7	TRM 76174	Neorhizobium alkalisol CCBAU 01393i	98.84	—	—	—	+	+	—	+++	+++	+++	+
8	TRM 76175	Rhizobium rosettiformans W₃	99.06	—	—	—	+	+	—	++	++	—	—
9	TRM 76101	Pseudochrobactrum asaccharolyticum DSM 25619	99.18	—	+	++	++		++	+++	—	—	—
10	TRM 76159	Phyllobacterium myrsinacearum DSM 5892	99.41	—	—	+	+	—	+	++	—	+++	+++
11	TRM 76062	Bacillus rugosus SPB7	99.86	—	—	—	+	+	+	—	—	—	—
12	TRM 76132	Rhizobium tarimense PL-41	99.56	—	+	—	—	—	—	+	+++	++	++
13	TRM 76072	Streptomyces tunisiensis CN-207	98.98	++	+++	—	—	—	—	—	—	—	—
14	TRM 76100	Achromobacter marplatensis B2	99.71	—	+	—	+	+	—	—	+++	—	++
15	TRM 76153	Bacillus cabrialesii TE3	99.79	—	—	—	—	—	—	++	+++	+	++

序号	菌株编号	相似菌株	相似性(%)	抑菌圈直径类型(mm)
序号	菌株编号	相似菌株	相似性(%)	S.a	P.a	E.c	K.p	E.a	S.t	C.a	A.t	V.d	F.o
1	TRM 76131	Cellulosimicrobium marinum RS-7-4	98.99	—	—	+	—	+	+	++	—	+++	+
2	TRM 76130	Rhizobium tarimense PL-41	99.71	++	—	+	—	—	+	+++		+++	++
3	TRM 76129	Salmonella enterica subsp.entericaL T2	99.23	—	—	—	+	+	+	++	++	++	—
4	TRM 76146	Rhizobium tarimense PL-41	99.05	—	—	+	—	+	+	—	+	+	—
5	TRM 76147	Microbacterium petrolearium LAM0410	98.56	—	+	—	—	—	—	+	+++	+++	+
6	TRM 76156	Rhizobium tarimense PL-41	98.84	—	—	—	—	—	—	+++	+++	+++	+
7	TRM 76174	Neorhizobium alkalisol CCBAU 01393i	98.84	—	—	—	+	+	—	+++	+++	+++	+
8	TRM 76175	Rhizobium rosettiformans W₃	99.06	—	—	—	+	+	—	++	++	—	—
9	TRM 76101	Pseudochrobactrum asaccharolyticum DSM 25619	99.18	—	+	++	++		++	+++	—	—	—
10	TRM 76159	Phyllobacterium myrsinacearum DSM 5892	99.41	—	—	+	+	—	+	++	—	+++	+++
11	TRM 76062	Bacillus rugosus SPB7	99.86	—	—	—	+	+	+	—	—	—	—
12	TRM 76132	Rhizobium tarimense PL-41	99.56	—	+	—	—	—	—	+	+++	++	++
13	TRM 76072	Streptomyces tunisiensis CN-207	98.98	++	+++	—	—	—	—	—	—	—	—
14	TRM 76100	Achromobacter marplatensis B2	99.71	—	+	—	+	+	—	—	+++	—	++
15	TRM 76153	Bacillus cabrialesii TE3	99.79	—	—	—	—	—	—	++	+++	+	++

柽柳根际土壤放线菌的分离及拮抗活性筛选

Isolation and antagonistic activity screening of Actinomycetes from rhizosphere soil of Tamarix

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