核桃林间作西红花对其土壤微生物数量的动态变化

doi:10.6048/j.issn.1001-4330.2023.08.024

新疆农业科学 ›› 2023, Vol. 60 ›› Issue (8): 2022-2027.DOI: 10.6048/j.issn.1001-4330.2023.08.024

核桃林间作西红花对其土壤微生物数量的动态变化

徐静¹^,²(), 石书兵¹, 秦小钢², 朱军¹^,²()

1.新疆农业大学农学院,乌鲁木齐 830052
2.新疆维吾尔自治区中药民族药研究所,乌鲁木齐 830002

收稿日期:2022-11-23 出版日期:2023-08-20 发布日期:2023-08-14
通信作者: 朱军(1982-),男,新疆人,研究员,硕士生导师,研究方向为新疆中药民族药资源及栽培,(E-mail)285362468@qq.com
作者简介:徐静(1999-),女,河南濮阳人,硕士研究生,研究方向为作物栽培,(E-mail)1912358430@qq.com
基金资助:
中国医学科学院中央级公益性科研院所基本科研业务费项目“管花肉苁蓉和西红花种植关键技术研究及推广示范”(2020-RW360-002);新疆维吾尔自治区公益性科研院所基本科研业务费项目“西红花林下种植关键技术研究及应用”(ky2021106)

Walnut forests intercropping saffron on Its soil microorganism's dynamic change in quantity study

XU Jing¹^,²(), SHI Shubing¹, QIN Xiaogang², ZHU Jun¹^,²()

1. College of Agronomy, Xinjiang Agricultural University, Urumqi 830052, China
2. Xinjiang Research Institutes of Traditional Chinese Materia Medica and Ethnodrug, Urumqi 830002, China

Received:2022-11-23 Online:2023-08-20 Published:2023-08-14
Correspondence author: ZHU Jun (1982-), male, researcher, master tutor, doctor, mainly engaged in the research of TCM ethnic medicine resources and cultivation in Xinjiang,(E-mail)285362468@qq.com
Supported by:
Research and Promotion Demonstration of Key Technologies of Cistanche and Safflower Planting of Pipe Flower(2020-RW360-002);Research and Application of Key Technologies of Planting under the Saffron Forest(ky2021106)

摘要/Abstract

摘要：

【目的】分析核桃西红花间作模式下土壤微生物数量的动态,研究核桃林下间作西红花对其土壤微生物数量的影响,为新型核桃间作西红花种植模式提供理论依据和实践参考。【方法】选取15年树龄核桃林下间作西红花为处理组(H15),以15年树龄核桃单作(HCK)和西红花单作(CK)为2个对照,采用稀释分离法测定不同处理下西红花生长期土壤中真菌、细菌和放线菌数量,并通过SPSS软件分析土壤中微生物数量的动态变化规律。【结果】(1)西红花生长期,土壤微生物数量变化规律总体呈现先升高后下降的趋势,在3月微生物数量达到峰值,与对照组比较,核桃林间作西红花其土壤中微生物数量显著提高。(2)在西红花生长旺盛期3月H15微生物总量为33.21×10⁵ cfu/g,分别是CK和HCK的1.55倍和7.27倍,差异性显著;H15的真菌菌落数量分别较CK对照组提高61.2%,较HCK对照组提高72.43%,达到显著性差异;间作模式下细菌数量为33.05%,分别较CK和HCK提高36.66%和86.39%,均达到显著性差异;H15放线菌菌落数较HCK提高53.58%,差异性显著(P<0.05),较CK提高9.59%,无显著差异。且CK放线菌数量较HCK提高48.65%,差异显著。(3)不同生长年限核桃林间作西红花处理模式下土壤中微生物总量与细菌、真菌和放线菌菌落数量变化趋势一致,依次为H15>CK>HCK。【结论】核桃间作西红花种植模式,明显优于核桃单作与西红花单作种植模式,且西红花单作模式下的微生物数量高于核桃间作模式,种植西红花能够显著增加土壤微生物数量,影响土壤肥力的转化,且以核桃间作西红花模式最优。

关键词: 核桃林间作; 西红花; 土壤微生物; 数量动态

Abstract:

【Objective】 To clarify the effect of intercropping saffron under walnut forest on the number of soil microorganisms through the dynamic determination of the number of soil microorganisms under the intercropping mode of walnut saffron,which can provide theoretical basis and practical reference for new walnut between saffron planting pattern.【Methods】 15-year old walnut intercropping saffron group (H15), with 15 years walnut singles (HCK) and saffron (CK) were selected as two controls to determine the number of fungi, bacteria and actinobacteria in the soil under different treatments, and analyze the dynamic changes of the soil by SPSS software.【Results】 (1) In the growing period of saffron, the change pattern of soil microbial number showed a trend of rising first and then decreasing, and in March, the number of microorganisms reached the peak.Compared with the control group, the number of microorganisms in the soil of walnut forest increased significantly.(2) The total H15 microorganism in March was 33.21×10⁵ cfu/g, the 1.55-fold and 7.27-fold values of CK and HCK, respectively, The difference was significant; The number of fungal colonies in H15 increased by 61.2% compared with CK controls, at a 72.43% higher rate than the HCK control group, achieving significant differences; The number of bacteria in the intercropping mode was 33.05%, 36.66% and 86.39% improvement over CK and HCK, respectively, all have reaching significant differences; The colony number of H15 Actinobacteria was increased by 53.58% compared with HCK, The difference was significant (P<0.05), a 9.59% improvement over CK with no significant differences.Moreover, the number of CK actinobacteria increased by 48.65% compared with HCK, with significant differences.(3) The total amount of microorganisms in the soil of different growth years was consistent with the number of bacteria, fungi and actinobacteria colonies, namely: H15> CK> HCK;【Conclusion】 Walnut between saffron planting pattern is obviously better than walnut single and saffron planting pattern, and the number of microorganisms higher than walnut intercropping pattern, which shows that planting saffron can significantly increase the number of soil microorganisms, affect the transformation of soil fertility, so it can be concluded that walnut between saffron model is optimal.

Key words: walnut intercropping; saffron; soil microorganisms; quantity dynamic

中图分类号:

S188
S344.2

徐静, 石书兵, 秦小钢, 朱军. 核桃林间作西红花对其土壤微生物数量的动态变化[J]. 新疆农业科学, 2023, 60(8): 2022-2027.

XU Jing, SHI Shubing, QIN Xiaogang, ZHU Jun. Walnut forests intercropping saffron on Its soil microorganism's dynamic change in quantity study[J]. Xinjiang Agricultural Sciences, 2023, 60(8): 2022-2027.

图/表 4

图1 不同处理条件下微生物总量比较注:图中不同小写字母表示处理间在0.05水平上差异显著(P<0.05)。HCK:15年核桃林单作(以下简称核桃单作); CK:西红花单作;H15:15年核桃林间作西红花,下同

Fig.1 Comparison of total microbial output under different treatment conditions Note:Different lowercase letters in Fig indicate significant treatment differences at the 0.05 level (P<0.05).HCK:15-year walnut forest (hereinafter referred to as walnut forest); CK: saffron; H15: 15 years between the walnut forest for saffron, the same as below

图2 西红花土壤的真菌数量的比较

Fig.2 Comparison of the number of fungi in safflower soil

图3 西红花土壤的细菌数量的比较

Fig.3 Comparison of bacterial numbers in saffron soil

图4 西红花土壤的放线菌数量比较

Fig.4 Comparison of actinomycete numbers in safflower soil

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核桃林间作西红花对其土壤微生物数量的动态变化

Walnut forests intercropping saffron on Its soil microorganism's dynamic change in quantity study

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