二甲戊灵对龙葵苗期主要生长发育和生理指标的影响

doi:10.6048/j.issn.1001-4330.2023.08.023

摘要/Abstract

摘要：

【目的】研究喷施二甲戊灵对龙葵苗期主要生长发育及其生理指标的影响。【方法】采用温室盆栽法,喷施不同浓度(0、300、600、900、1 200、1 500 g.a.i/hm²)二甲戊灵,于施药后不同时间内(5、10、15、20、25、30 d)测定龙葵的株高、茎粗、叶面积、地上部分鲜重及叶绿素含量、可溶性糖含量、可溶性蛋白含量。【结果】龙葵的生长指标和生理指标随二甲戊灵剂量升高整体呈下降趋势,当浓度为1 500 g.a.i/hm²时对其影响最为严重。其中在处理第30 d时龙葵株高、叶面积、地上部分鲜重最小,相比对照存在显著差异,分别为2.02 cm、0.32 cm²、0.47 g。在处理前15 d时茎粗相比对照明显增粗,随着龙葵后期的生长对照茎粗明显高于各处理。与对照相比,可溶性糖、可溶性蛋白、叶绿素含量在第30 d时达到最小值3.12、0.06、0.62 mg/g,且分别减少了7.82、0.05、1.05 mg/g。【结论】二甲戊灵对龙葵苗期生长发育指标及生理指标具有显著影响,随药剂剂量浓度升高其各项指标整体呈现出下降趋势,且高浓度对其影响最为严重。

关键词: 龙葵; 二甲戊灵; 生长发育; 生理指标

Abstract:

【Objective】 To clarify the effect of spraying pendimethalin on the main growth and development and physiological indicators of Solanum nigrum L.【Methods】 Plant height, stem thickness, leaf area, fresh weight of above-ground parts, chlorophyll content, soluble sugar content and soluble protein content of Solanum nigrum L.were measured at spraying different concentrations of pendimethalin (0, 300, 600, 900, 1,200,1,500 g.a.i/hm²) at different times (5, 10, 15, 20, 25,30 d) after spraying pesticides using the greenhouse pot method.【Results】 The growth and physiological indicators of Solanum nigrum L.showed an overall decreasing trend with increasing dosage of pendimethalin, and the most serious effect was observed when the concentration was 1,500 g.a.i/hm².Among them, on the 30th day of treatment, the plant height, leaf area, and fresh weight of the above-ground part of the Solanum nigrum L.were the smallest, which were significantly different from that of the control, which were 2.02 cm, 0.32 cm², and 0.47 g, respectively.The stem thickness was significantly thicker compared to the control at 15 d before treatment, and with the later growth of Solanum nigrum L.the control stem thickness was significantly higher than those of the treatments.Compared with the control, the soluble sugar, soluble protein, and chlorophyll content reached the minimum 3.12, 0.06, and 0.62 mg/g at the 30th day, and decreased by 7.82, 0.05, 1.05 mg/g, respectively.【Conclusion】 Pendimethalin has a significant effect on the main growth and development and physiological indexes of Solanum nigrum L., and the overall physiological and biochemical indexes show a decreasing trend with increasing dosage of concentrations, and the most serious effect has been observed at high concentrations.

Key words: Solanum nigrum L.; pendimethalin; growth and development; physiological indicators

中图分类号:

S641.9

魏迎凤, 张全成, 查慧, 王小丽, 王俊刚. 二甲戊灵对龙葵苗期主要生长发育和生理指标的影响[J]. 新疆农业科学, 2023, 60(8): 2013-2021.

WEI Yingfeng, ZHANG Quancheng, ZHA Hui, WANG Xiaoli, WANG Jungang. Effect of pendimethalin on the main growth and development and physiological indicators of Solanum nigrum L.[J]. Xinjiang Agricultural Sciences, 2023, 60(8): 2013-2021.

图/表 6

表1 不同浓度二甲戊灵下龙葵株高变化

Tab.1 The effect of pendimethalin on the plant height of Solanum nigrum L.(cm)

浓度 Concentration (g.a.i/hm²)	药后时间 Post-herbicides time
浓度 Concentration (g.a.i/hm²)	5 d	10 d	15 d	20 d	25 d	30 d
0	4.67±1.00^a	6.15±0.84^a	8.43±0.91^a	11.09±1.17^a	14.52±1.65^a	20.13±2.48^a
300	1.98±0.31^b	2.44±0.52^b	2.93±0.88^b	4.13±1.26^b	4.22±1.53^b	5.54±2.13^b
600	1.56±0.34^c	1.89±0.31^c	1.96±0.26^c	2.31±0.24^c	2.39±0.56^c	2.30±0.36^c
900	1.31±0.27^cd	1.96±0.38^c	1.89±0.23^c	2.13±0.18^cd	2.18±0.27^c	2.15±0.16^c
1 200	1.18±0.21^d	1.90±0.21^c	1.81±0.22^c	1.93±0.14^cd	1.96±0.32^c	2.06±0.17^c
1 500	0.97±0.20^d	1.46±0.16^d	1.67±0.13^c	1.77±0.11^d	1.90±0.10^c	2.02±0.09^c

图1 不同浓度二甲戊灵下不同时期龙葵的长势变化注:A为施药后5 d龙葵的长势情况;B为施药后10 d龙葵的长势情况;C为施药后15 d龙葵的长势情况;D为施药后20 d龙葵的长势情况;E为施药后25 d龙葵的长势情况;F为施药后30 d龙葵的长势情况

Fig.1 Effect of pendimethalin of different concentrations on the growth of Solanum nigrum L.in different periods Note: A is the growth condition of the Solanum nigrum L.5 d after herbicides ; B is the growth condition of the Solanum nigrum L.10 d after herbicides ; C is the growth condition of the Solanum nigrum L.15 d after herbicides ; D is the growth condition of the Solanum nigrum L.20 d after herbicides ; E is the growth condition of the Solanum nigrum L.25 d after herbicides ; F is the growth condition of the Solanum nigrum L.30 d after herbicides.

表2 不同浓度二甲戊灵下龙葵茎粗变化

Tab.2 The effect of pendimethalin on the stem thickness of Solanum nigrum L.(cm)

浓度 Concentration (g.a.i/hm²)	药后时间 Post-herbicides time
浓度 Concentration (g.a.i/hm²)	5 d	10 d	15 d	20 d	25 d	30 d
0	0.05±0.01^c	0.06±0.01^b	0.10±0.01^c	0.16±0.03^a	0.21±0.03^a	0.25±0.03^a
300	0.08±0.02^b	0.12±0.02^a	0.13±0.02^ab	0.12±0.02^bc	0.13±0.01^b	0.13±0.02^b
600	0.11±0.02^a	0.12±0.02^a	0.12±0.02^b	0.12±0.02^bcd	0.12±0.01^c	0.14±0.01^b
900	0.11±0.02^a	0.12±0.02^a	0.12±0.02^b	0.11±0.01^cd	0.13±0.01^bc	0.13±0.01^b
1 200	0.11±0.02^a	0.12±0.02^a	0.12±0.02^ab	0.11±0.01^d	0.12±0.01^c	0.14±0.02^b
1 500	0.11±0.02^a	0.13±0.01^a	0.13±0.01^a	0.13±0.01^b	0.13±0.01^bc	0.14±0.02^b

表3 不同浓度二甲戊灵下龙葵地上部分鲜重变化

Tab.3 The effect of pendimethalin on the fresh weight of the ground part of Solanum nigrum L.(g)

浓度 Concentration (g.a.i/hm²)	药后时间 Post-herbicides time
浓度 Concentration (g.a.i/hm²)	5 d	10 d	15 d	20 d	25 d	30 d
0	0.26±0.05^a	0.41±0.07^a	0.77±0.13^a	1.50±0.46^a	3.92±1.39^a	10.06±1.75^a
300	0.21±0.04^b	0.32±0.06^b	0.48±0.22^b	0.72±0.11^b	2.02±0.28^b	4.10±0.65^b
600	0.20±0.04^b	0.33±0.10^b	0.47±0.13^b	0.47±0.11^c	1.09±0.29^c	2.03±0.36^c
900	0.22±0.05^b	0.31±0.05^bc	0.38±0.10^c	0.33±0.08^cd	0.57±0.14^d	0.98±0.12^d
1 200	0.21±0.04^b	0.32±0.06^bc	0.31±0.09^c	0.32±0.08^d	0.37±0.09^d	0.64±0.13^d
1 500	0.13±0.03^c	0.27±0.06^c	0.33±0.08^c	0.33±0.09^cd	0.36±0.03^d	0.47±0.10^d

表4 不同浓度二甲戊灵下龙葵叶面积变化

Tab.4 The effect of pendimethalin on the leaf area of Solanum nigrum L.(cm2)

浓度 Concentration (g.a.i/hm²)	药后时间Post-herbicides time
浓度 Concentration (g.a.i/hm²)	5 d	10 d	15 d	20 d	25 d	30 d
0	0.34±0.12^a	0.62±0.16^a	0.83±0.21^a	0.83±0.19^a	1.11±0.21^a	1.40±0.27^a
300	0.16±0.05^c	0.26±0.10^bc	0.28±0.07^b	0.27±0.08^b	0.27±0.05^b	0.34±0.07^b
600	0.23±0.08^b	0.29±0.08^b	0.30±0.07^b	0.28±0.07^b	0.29±0.08^b	0.33±0.08^b
900	0.15±0.08^c	0.21±0.07^c	0.26±0.07^b	0.30±0.07^b	0.32±0.07^b	0.31±0.09^b
1 200	0.12±0.07^c	0.21±0.05^c	0.25±0.06^b	0.28±0.08^b	0.26±0.06^b	0.26±0.07^b
1 500	0.15±0.06^c	0.19±0.03^c	0.22±0.05^b	0.26±0.06^b	0.29±0.06^b	0.32±0.06^b

图2 不同浓度二甲戊灵下龙葵生理指标变化注:2-1为二甲戊灵对龙葵叶绿素含量的影响;2-2为二甲戊灵对龙葵可溶性糖含量的影响;2-3为二甲戊灵对龙葵可溶性蛋白含量的影响。图中数值0、300、600、900、1 200、1 500分别表示喷施二甲戊灵浓度为0、300、600、900、1 200、1 500 g.a.i./hm2,小写字母表示在0.05水平下差异显著。

Fig.2 Effect of Pendimethalin on the physiological indicators of Solanum nigrum L. Note: 2-1 is the effect of pendimethalin on the chlorophyll content of Solanum nigrum L.;2-2 is the effect of pendimethalin on the soluble sugar content of Solanum nigrum L.;2-3 is the effect of pendimethalin on the soluble protein content of Solanum nigrum L.Values 0, 300, 600, 900, 1,200, 1,500 in the figure indicate that the spraying pendimethalin concentration is 0, 300, 600, 900, 1,200, 1,500 g.a.i/hm2, and lowercase letters indicate significant differences at the 0.05 level.

参考文献 29

[1]	CHUN Z Z, CHEN J Y. Novel α-Tubulin Mutations Conferring Resistance to Dinitroaniline Herbicides in Lolium rigidum[J]. Frontiers in Plant Science, 2018, 9: 1-12. DOI URL
[2]	Breeden S M, Brosnan J T, Breeden G K, et al. Controlling Dinitroaniline-Resistant Goosegrass (Eleusine indica) in Turfgrass[J]. Weed Technology, 2017, 31(6): 12-16.
[3]	Chen J Y, Yu Q, Patterson E, et al. Dinitroaniline Herbicide Resistance and Mechanisms in Weeds[J]. Frontiers in Plant Science, 2021, 12: 634018-634018. DOI URL
[4]	连玉朱, 王金信, 李浙江, 等. 几种棉田除草剂大田防除效果及其对棉花的安全性测定[J]. 农药, 2006,(4): 270-271,277.
	LIAN Yuzhu, WANG Jinxin, LI Zhejiang, et al. Field control effect of several herbicides in cotton field and their safety to cotton[J]. Agrochemicals, 2006,(4): 270-271,277.
[5]	赵冰梅, 朱玉永, 王林. 丙炔氟草胺与二甲戊灵混配使用对棉田杂草的防除效果及棉花安全性研究[J]. 植物保护, 2021, 47(3): 250-255,264.
	ZHAO Bingmei, ZHU Yuyong, WANG Lin. Control effect of mixed use of flumioxazin and pendimethalin on weed in cotton fields and safety to cotton[J]. Acta Phytophylacica Sinica, 2021, 47(3): 250-255,264.
[6]	朱玉永, 赵冰梅, 王林. 新疆棉田杂草发生与防除现状及对策[J]. 中国棉花, 2021, 48(2): 1-7.
	ZHU Yuyong, ZHAO Bingmei, WANG Lin. Current status of weed occurrence and control in cotton field of Xinjiang and its countermeasures[J]. China Cotton, 2021, 48(2): 1-7.
[7]	冯宏祖, 王兰. 新疆南部棉区棉田杂草调查[J]. 安徽农业科学, 2008,(7): 2819-2820,2986.
	FENG Hongzhu, WANG Lan. Investigation on weeds in cotton fields in southern Xinjiang[J]. Journal of Anhui Agricultural Sciences, 2008,(7): 2819-2820,2986.
[8]	郝彦俊, 李广阔, 王剑, 等. 新疆棉田杂草发生及防治[J]. 新疆农业科学, 2003,(1): 24-25.
	HAO Yanjun, LI Guangkuo, WANG Jian. Occurrence and control of weeds in cotton fields in Xinjiang[J]. Xinjiang Agricultural Sciences, 2003,(1): 24-25.
[9]	黄红娟, 张朝贤, 姜翠兰, 等. 北疆棉田杂草多样性及群落组成[J]. 杂草学报, 2020, 38(1): 7-13.
	HUANG Hongjun, ZHANG Chaoxian, JIANG Cuilan, et al. Diversity and community composition of weeds in cotton fields of northern Xinjiang[J]. Journal of Weed Science, 2020, 38(1): 7-13.
[10]	蒋成国, 刘彤, 张建萍, 等. 滴灌下新疆北部棉田杂草土壤种子库的时空变化[J]. 生态学报, 2009, 29(6): 3081-3089.
	JIANG Chenguo, LIU Tong, ZHANG Jianping, et al. Spatiotemporal variation of weed seed banks under drip irrigation in cotton fields of Northern Xinjiang[J]. Acta Ecologica Sinica, 2009, 29(6): 3081-3089.
[11]	张强, 李红, 赵冰梅, 等. 新疆兵团机采棉田杂草种类及群落结构调查[J]. 杂草学报, 2021, 39(1): 29-37.
	ZHANG Qiang, LI Hong, ZHAO Bingmei, et al. Investigation on weed species and community structure in cotton field of Xinjiang Corps[J]. Journal of Weed Science, 2021, 39(1): 29-37.
[12]	张强, 田英, 朱玉永, 等. 新疆兵团主要植棉区机采棉田杂草调查[J]. 中国棉花, 2020, 47(5): 13-16.
	ZHANG Qiang, TIAN Ying, ZHU Yuyong, et al. Investigation on weeds in mechanical cotton fields in main cotton planting areas of Xinjiang Corps[J]. China Cotton, 2020, 47(5): 13-16.
[13]	魏建华, 张建云, 马冬梅. 新疆昌吉州棉田杂草发生演替规律调查研究[J]. 中国棉花, 2016, 43(1): 31-33.
	WEI Jianhua, ZHANG Jianyun, MA Dongmei. Investigation and study on law of succession of weeds in cotton fields in Changji, Xinjiang[J]. China Cotton, 2016, 43(1): 31-33.
[14]	赵冰梅, 朱玉永, 张强. 乙氧氟草醚和二甲戊灵防除覆膜棉田龙葵效果[J]. 农药, 2017, 56(8): 616-618.
	ZHAO Bingmei, ZHU Yuyong, ZHANG Qiang. Efficacy of oxyfluorfen and pendimethalian on Solanum nigrum in film covered cotton field[J]. Agrochemicals, 2017, 56(8): 616-618.
[15]	谢桂英, 游秀峰, 孙淑君, 等. 龙葵种子休眠解除方法研究[J]. 杂草科学, 2013, 31(1): 37-39.
	XIE Guiying, YOU Xiufeng, SUN Shujun, et al. Research on the method of solving dormancy of Solanum nigrum L.Seed[J]. Weeds Science, 2013, 31(1): 37-39.
[16]	李合生. 植物生理生化实验原理和技术[M]. 北京: 高等教育出版社, 2000.
	LI Hesheng. Principles and techniques of plant physiological and biochemical experiments[M]. Beijing: Higher Education Press, 2000.
[17]	刘阳. 两种苯氧羧酸类除草剂对谷子生理特性及安全性的影响[D]. 晋中: 山西农业大学, 2016.
	LIU Yang. Effects of two phenoxycarboxylic herbicides on physiological characteristics and safety of foxtail millet[D]. Jinzhong: Shanxi Agricultural University, 2016.
[18]	Song N, Yang Z, Zhou L, et al. Effect of dissolved organic matter on the toxicity of chlorotoluron to Triticumae stivum[J]. Journal of Environmental Science, 2006, 18(1): 101-108.
[19]	王丹. 适于大蒜茎叶处理的除草剂筛选及其生理效应研究[D]. 泰安: 山东农业大学, 2018.
	WANG Dan. Screening of herbicides suitable for garlic stem and leaf treatment and their physiological effects[D]. Tai’an: Shandong Agricultural University, 2018.
[20]	徐汉虹. 植物化学保护[M]. 北京: 中国农业出版社, 2007: 197-198.
	XU Hanhong. Plant Chemical Protection[M]. Beijing: China Agriculture Press, 2007: 197-198.
[21]	张少逸, 张朝贤, 王金信, 等. 五种土壤处理除草剂对刺萼龙葵的生物活性研究[J]. 华北农学报, 2012, 27(S1): 382-385. DOI
	ZHANG Shaoyi, ZHANG Chaoxian, WANG Jinxing, et al. Study on biological activities of five herbicides for soil treatment to Solanum rostratum Dunal[J]. Acta Agriculturae Boreali-Sinica, 2012, 27(S1): 382-385.
[22]	刘韶光. 氟乐灵和二甲戊灵对谷子安全性及田间杂草防效的研究[D]. 晋中: 山西农业大学, 2019.
	LIU Shaoguang. Study on the safety of trifluralin and pendimethalin to millet and the control effect of weeds in the field[D]. Jinzhong: Shanxi Agricultural University, 2019.
[23]	赵江涛, 李晓峰, 李航, 等. 可溶性糖在高等植物代谢调节中的生理作用[J]. 安徽农业科学, 2006, 4(24): 6423-6425,6427.
	ZHAO Jiangtao, LI Xiaofeng, LI Hang, et al. The physiological role of soluble sugar in metabolic regulation of higher plants[J]. Journal of Anhui Agricultural Sciences, 2006, 4(24): 6423-6425,6427.
[24]	武辉, 张巨松, 石俊毅, 等. 棉花幼苗对不同程度低温逆境的生理响应[J]. 西北植物学报, 2013, 33(1): 74-82.
	WU Hui, ZHANG Jusong, SHI Junyi, et al. Physiological response of cotton seedlings to different degree of low temperature stress[J]. Acta Botanica Boreali-Occidentalia Sinica, 2013, 33(1): 74-82.
[25]	王正贵, 周立云, 郭文善, 等. 除草剂对小麦光合特性及叶绿素荧光参数的影响[J]. 农业环境科学学报, 2011, 30(6): 1037-1043.
	WANG Zhenggui, ZHOU Liyun, GUO Wenshsn, et al. Effects of herbicides on photosynthetic characteristics and chlorophyll fluorescence parameters of wheat[J]. Journal of Agro-Environment Science, 2011, 30(6): 1037-1043. DOI URL
[26]	李贵, 吴竞仑. 除草剂对作物生理生化指标的影响[J]. 安徽农业科学, 2007,(29): 9157-9159.
	LI Gui, WU Jinglong. Effect of herbicide on crop physiology and biochemistry[J]. Journal of Anhui Agricultural Sciences, 2007,(29): 9157-9159.
[27]	彭俊, 宋志龙, 孟新刚, 等. 除草剂对藨草生理生化指标的影响[J]. 西北农业学报, 2013, 22(9): 192-197.
	PENG Jun, SONG Zhilong, MENG Xingang, et al. The effect of herbicides on physiological and biochemical indexes of Scirpus triqueter L.[J]. Acta Agriculturae Boreali-occidentalis Sinica, 2013, 22(9): 192-197.
[28]	朱诗禹, 崔娟, 徐伟, 等. 精异丙甲草胺苗前封闭处理对大豆苗期生长及其生理生化指标的影响[J]. 植物保护学报, 2016, 43(4): 677-682.
	ZHU Shiyu, CUI Juan, XU Wei, et al. Effect of the treatment with S-metolachlor befor seeding emergence on the soybean growth and physiological and biochemical indexes[J]. Acta Phytophylacica Sinica, 2016, 43(4): 677-682.
[29]	钱兰娟, 袁树忠, 高颜. 除草剂炔草酯对不同小麦品种幼苗期叶片抗氧化特性的影响[J]. 麦类作物学报, 2014, 34(5): 674-679.
	QIAN Lanjuan, YUAN Shuzhong, GAO Yan. Effects of the herbicide Clodinafop-propargyl on the anti-oxidant characteristics of different wheat cultivars at the seedling stage[J]. Journal of Triticeae Crops, 2014, 34(5): 674-679.