Xinjiang Agricultural Sciences ›› 2025, Vol. 62 ›› Issue (1): 146-160.DOI: 10.6048/j.issn.1001-4330.2025.01.018

• Cultivation Physiology·Physiology and Biochemistry·Germplasm Resources·Molecular Genetics·Soil Fertilizer • Previous Articles     Next Articles

Effects of different saline and alkaline stress on the proteome of cotton root system

SUN Caiqin(), WU Jia, HUANG Hai, GUO Jiaxin, MIN Wei, GUO Huijuan()   

  1. Agricultural College, Shihezi University, Shihezi Xinjiang 832000, China
  • Received:2024-07-25 Online:2025-01-20 Published:2025-03-11
  • Correspondence author: GUO Huijuan
  • Supported by:
    National Natural Science Foundation of China(32160742);Open Fund of Key Laboratory of Northwest Oasis Agro-Environment of Ministry of Agriculture and Rural Affairs of China(XBLZ-20214);Youth Innovation Talent Cultivation Program of Shihezi University(CXPY202111);Scientific Research Starting Foundation for High Level Talents of Shihezi University(RCZK202017)

不同盐碱胁迫对棉花根系蛋白质组的影响

孙彩琴(), 吴佳, 黄海, 郭家鑫, 闵伟, 郭慧娟()   

  1. 石河子大学农学院,新疆石河子 832000
  • 通讯作者: 郭慧娟
  • 作者简介:孙彩琴(2001-),女,甘肃通渭人,本科生,研究方向为土壤肥力与调控,(E-mail)scaiq0927@163.com
  • 基金资助:
    国家自然科学基金项目(32160742);农业农村部西北绿洲农业环境重点实验室开放基金(XBLZ-20214);石河子大学青年创新人才培养计划(CXPY202111);石河子大学高层次人才科研启动资金专项(RCZK202017)

Abstract:

【Objective】 Exploring the mechanism of cotton tolerance to salt and alkali stress plays an important role in improving cotton yield in Alkali soil in Xinjiang. 【Methods】 This study used cotton as the experimental material, and set up three treatments: control (CK), salt stress (NaCl, CS), and alkali stress (NaHCO3+Na2CO3, AS). Then, TMT technology was used to analyze the changes in protein expression in cotton roots under salt and alkali stress, and bioinformatics analysis was performed on the screened differential proteins. 【Results】 The results showed that under salt stress, the activities of Hexokinase, Phosphofructokinase, Pyruvate kinase and citrate synthase in roots were significantly reduced by 12.0%, 9.9%, 9.7%, and 32.8%, respectively. Under alkali stress, the activities of Hexokinase, Phosphofructokinase, Pyruvate kinase, Malate dehydrogenase, Glutamate dehydrogenase and cereal Transaminase in roots were significantly increased by 10.9%, 5.9%, 10.4%, 45.1%, 26.3%, and 23.4%, respectively. A total of 1725 differentially expressed proteins were screened under salt stress, including 508 upregulated differentially expressed proteins and 1217 downregulated differentially expressed proteins. Among them, there were 10 upregulated differentially expressed proteins and 43 downregulated differentially expressed proteins in energy metabolism, 14 upregulated differentially expressed proteins and 76 downregulated differentially expressed proteins in amino acid metabolism, 45 upregulated differentially expressed proteins and 84 downregulated differentially expressed proteins in genetic information processing, and 13 upregulated differentially expressed proteins and 29 downregulated differentially expressed proteins in signal transduction; Under alkaline stress, there were a total of 75 differentially expressed proteins, including 30 upregulated differentially expressed proteins and 45 downregulated differentially expressed proteins. Among them, there were only 2 downregulated differentially expressed proteins in energy metabolism, 1 upregulated differentially expressed protein in amino acid metabolism, and 3 downregulated differentially expressed proteins. No differentially expressed proteins were identified in genetic information processing and signal transduction. 【Conclusion】 The results indicate that salt stress significantly inhibits energy metabolism, amino acid metabolism, genetic information processing and signal transduction in cotton roots, while alkali stress has no significant effect on genetic information processing and signal transduction.

Key words: salt stress; alkali stress; cotton; proteome; amino acid metabolism; energy metabolism

摘要:

【目的】 探究棉花耐受盐碱胁迫的机制,为提高新疆盐碱地棉花产量提供依据。【方法】 以棉花为材料,设置对照(CK)、盐胁迫(NaCl,CS)和碱胁迫(NaHCO3+Na2CO3,AS)3个处理,采用TMT技术分析盐胁迫和碱胁迫下棉花根系中蛋白表达的变化,对筛选出的差异蛋白进行生物信息学分析。【结果】 盐胁迫下根中己糖激酶、磷酸果糖激酶、丙酮酸激酶和柠檬酸合酶活性分别显著降低12.0%、9.9%、9.7%和32.8%,碱胁迫下根中己糖激酶、磷酸果糖激酶、丙酮酸激酶、苹果酸脱氢酶、谷氨酸脱氢酶和谷草转氨酶活性分别显著增加10.9%、5.9%、10.4%、45.1%、26.3%和23.4%。在盐胁迫下共筛选出1 725个差异蛋白,包括508个上调差异蛋白和1217个下调差异蛋白,其中能量代谢有10个上调差异蛋白、43个下调差异蛋白,氨基酸代谢有14个上调差异蛋白、76个下调差异蛋白,遗传信息处理有45个上调差异蛋白、84个下调差异蛋白,信号传导有13个上调差异蛋白、29个下调差异蛋白;在碱胁迫下共75个差异蛋白,包括30个上调差异蛋白和45个下调差异蛋白,其中能量代谢只有2个下调差异蛋白,氨基酸代谢1个上调差异蛋白、3个下调差异蛋白,遗传信息处理和信号传导中未筛选出差异蛋白。【结论】 盐胁迫显著抑制棉花根系中的能量代谢,氨基酸代谢,遗传信息处理和信号传导,碱胁迫对遗传信息处理和信号传导无显著影响。

关键词: 盐胁迫, 碱胁迫, 棉花, 蛋白质组, 氨基酸代谢, 能量代谢

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