Effect of Low Temperature Stress at Bud Stage on Fluorescence Characteristics of Cotton

doi:10.6048/j.issn.1001-4330.2022.09.003

Xinjiang Agricultural Sciences ›› 2022, Vol. 59 ›› Issue (9): 2104-2113.DOI: 10.6048/j.issn.1001-4330.2022.09.003

• Cultivation Physiology·Physiology and Biochemistry·Crop Genetics and Breeding·Germplasm Resources • Previous Articles Next Articles

Effect of Low Temperature Stress at Bud Stage on Fluorescence Characteristics of Cotton

ZHONG Xinnian¹^,²(), LIANG Qigan³(), WEI Yinong¹, ZHAO Jianhua¹, MA Haixia¹, LI Zhibo¹()

1. School of Agriculture， Shihezi University/Key Laboratory of Oasis Ecological Agriculture of Xinjiang Corps， Xinjiang Shihezi 832003， China
2. Tropical Crop Service Center of Chengmai County， Chengmai Hainan 571900， China
3. Xinjiang Academy of Agricultural Sciences/Hainan Sanya Crop Breeding Test Center，Sanya Hainan 572014， China

Received:2021-11-21 Online:2022-09-20 Published:2023-01-16
Correspondence author: LIANG Qigan, LI Zhibo
Supported by:
National Natural Science Foundation of China(31560074);Open Project of the State Key Laboratory of Cotton Biology(CB2021A09);Animal and Plant Cultivation Project of Shihezi University(yzzx201602);Open Project of Key Laboratory of Oasis Ecological Agriculture of Xinjiang Production and Construction Corps(201902)

蕾期低温胁迫对棉花叶绿素荧光特性的影响

钟信念¹^,²(), 梁其干³(), 魏亦农¹, 赵建华¹, 马海霞¹, 李志博¹()

1.石河子大学农学院/新疆兵团绿洲生态农业重点实验室，新疆石河子 832003
2.海南省澄迈县热带作物服务中心，海南澄迈 571900
3.新疆农业科学院/海南三亚农作物育种试验中心，海南三亚 572014

通讯作者: 梁其干,李志博
作者简介:钟信念（1994-），女，海南临高人，硕士，研究方向为作物抗逆生理，（E-mail）2386224134@qq.com
基金资助:
国家自然科学基金(31560074);棉花生物学国家重点实验室开放课题(CB2021A09);石河子大学动植物培育专项(yzzx201602);新疆生产建设兵团绿洲生态农业重点实验室开放课题(201902)

Abstract

Abstract:

【Objective】 To analyze the effect of different low temperature stress on chlorophyll fluorescence characteristics of different cotton varieties at bud stage，explore the cold resistance response law of cotton at bud stage，and screen the indexes related to cold resistance of cotton at bud stage，so as to provide theoretical basis and technical guidance for identification of cold resistance resources and breeding of cold resistant cotton varieties. 【Methods】 Three low temperature gradients were set at bud stage：T₁（20℃/15℃，day/night），T₂（15℃/10℃），T₃（10℃/5℃） and CK （28℃/20℃）. 【Result】 The chlorophyll fluorescence parameters Fv，Fm，Fv/ Fo，qP and SPAD decreased，while NPQ and F o increased under low temperature stress. However，Fv/Fm increased at low temperature above 20℃/15℃ and 15℃/10℃，and decreased at low temperature below 10℃/5℃ and 15℃/10℃. 【Conclusion】 The degree of low temperature stress can be judged by the fluorescence parameters such as Fm and Fo of cotton in bud stage.

Key words: cotton; low temperature stress; bud stage; chlorophyll fluorescence

摘要：

【目的】 分析棉花蕾期不同低温胁迫对不同棉花品种的叶绿素荧光特性的影响，研究棉花蕾期的抗冷响应规律，筛选棉花蕾期耐冷性相关的指标，为耐冷资源鉴定、耐冷棉花品种选育提供依据。【方法】 于棉花蕾期设置3个低温梯度：T₁（20℃/15℃，昼/夜）、T₂（15℃/10℃）、T₃（10℃/5℃）和对CK（28℃/20℃）处理。【结果】 蕾期棉花在低温胁迫条件叶绿素荧光参数Fv、Fm 、Fv/F₀、qP、SPAD下降，NPQ、F₀上升。而Fv/Fm在20℃/15℃以上低温和15℃/10℃低温初期升高；10℃/5℃以下低温和15℃/10℃低温后期下降。【结论】 可根据蕾期棉花的Fm、F₀等荧光参数来判断其受到的低温胁迫程度。

关键词: 棉花, 低温胁迫, 蕾期, 叶绿素荧光

CLC Number:

S502

ZHONG Xinnian, LIANG Qigan, WEI Yinong, ZHAO Jianhua, MA Haixia, LI Zhibo. Effect of Low Temperature Stress at Bud Stage on Fluorescence Characteristics of Cotton[J]. Xinjiang Agricultural Sciences, 2022, 59(9): 2104-2113.

钟信念, 梁其干, 魏亦农, 赵建华, 马海霞, 李志博. 蕾期低温胁迫对棉花叶绿素荧光特性的影响[J]. 新疆农业科学, 2022, 59(9): 2104-2113.

Figures/Tables 9

Fig. 1 Effect of low temperature stress for 15 days on cotton phenotype at bud stage

Fig. 2 T Effects of low temperature stress on initial fluorescence

Fig. 3 Effects of low temperature stress on maximum fluorescence yield Fm

Fig. 4 Effects of low temperature stress on i variable fluorescent Fv

Fig. 5 Effect of low temperature stress on potential photochemical efficiency Fv/Fo

Fig. 6 Effect of low temperature stress on maximum photochemical efficiency （Fv/FM））of PSII

Fig. 7 Effect of low temperature stress on photochemical quenching rate qP

Fig. 8 Effect of low temperature stress on NPQ

Fig. 9 Effects of low temperature stress on chlorophyll SPAD

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Effect of Low Temperature Stress at Bud Stage on Fluorescence Characteristics of Cotton

蕾期低温胁迫对棉花叶绿素荧光特性的影响

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