Abstract: 【Objective】 To understand the genetic and evolutionary relationship between ZmCDPK38 and homologous CDPKs from other plant species and to predict the function of ZmCDPK38 theoretically by comparing with homologous genes with close relatives and known functions and to determine the expression characteristics of ZmCDPK38 genes in different tissues and under different stress conditions.【Method】 The online tools and MEGA-X software were used for bioinformatic analysis. Gene expression analysis was performed by real-time PCR.【Result】 ZmCDPK38 shared highly identical amino acid sequences with ZmCDPK26 and other six CDPKs from other species, indicating their closest genetic relationship. ZmCDPK38 protein contained four classical conserved domains of CDPK family member, including Ser/Thr protein kinase domains. The quantitative PCR results indicated that the ZmCDPK38 gene was expressed in a tissue-specific manner, which showed high expression level in maize leaves and less mRNA accumulation in stem, root, ear and tassel. Meanwhile the up-regulated transcription was observed during the treatment of drought and salt stress. On the contrary, the expression level showed little difference in response to low and high temperature except an initially increasedmRNA accumulation at 3h and 6h time-point after low temperature treatment.【Conclusion】 ZmCDPK38 protein contains four classical conserved domains of CDPK family member indicating its protein kinase characters. And the transcriptional analysis demonstrated that the expression of ZmCDPK38 gene was induced in response to drought and salt stress, suggesting that it may play a role in the regulation of drought and salt tolerance.

Key words: maize; ZmCDPK38; abiotic stress; bioinformation; gene expression

摘要： 【目的】 研究ZmCDPK38与其它物种中同源基因之间的遗传进化关系,通过与亲缘关系较近且功能已知的同源基因比较,对ZmCDPK38的功能进行理论预判。确定ZmCDPK38基因在不同组织和不同胁迫条件下的表达特性。【方法】 生物信息学分析使用在线分析工具及MEGAX软件;基因表达分析采用Real-time PCR。【结果】 ZmCDPK38与ZmCDPK28及高粱等6个其它植物物种的CDPK的氨基酸序列具有较高的一致性,遗传进化关系上较近。氨基酸序列包含4个结构域,具有CDPKs家族氨基酸的典型特征,Ser/Thr蛋白激酶结构域相对保守。ZmCDPK38基因在叶片中的表达量最高,其次是茎部、根、雄穗及雌穗;受盐胁迫及干旱胁迫诱导,ZmCDPK38基因的表达水平显著上升,在低温和高温胁迫条件下基因表达未见明显变化。【结论】 ZmCDPK38基因作为CDPK家族成员,在蛋白序列和结构上具备典型的CDPK家族成员特征;ZmCDPK38基因响应干旱胁迫与盐胁迫,可能在干旱应答与盐胁迫应答反应中发挥一定的功能。

关键词: 玉米, ZmCDPK38, 非生物胁迫, 生物信息, 基因表达