A comparative study on reproductive performance, serum hormone levels and gene expression between Yili goose and hortobágy goose

doi:10.6048/j.issn.1001-4330.2023.05.027

Abstract

Abstract:

【Objective】 To study the differences between the production performance, hatching performance, serum reproductive hormone levels and the expression of genes related to goose reproductive performance in different tissues of Yili and Hortobágy geese in the hope of providing theoretical support for crossbreeding improvement of Yili geese.【Method】 In this experiment, 120 2-year-old Yili geese and 120 Hortobágy geese, each in good health, with consistent feeding conditions and in egg-laying stage, were selected for the determination of egg production, serum hormone levels and the expression of BMP2, BMP6, MIS, ENO1 and EP300 genes.【Result】 ①The egg production rate, daily egg production, average daily feed intake, average egg weight and egg shape of Yili geese were significantly or highly significantly lower than those of Khordaji geese (P<0.05 or P<0.01), and the material-to-egg ratio was significantly higher than that of Khordaji geese (P<0.01); in terms of hatching performance, the fertilization rate and fertilized egg hatching rate of Yili geese were significantly or highly significantly lower than those of Khordaji geese (P< 0.05 or P<0.01), and the rest of the indicators were not significantly different.②The serum levels of GnRH, LH, FSH, E2 and P4 in Yili geese were significantly or extremely significantly lower than those in Holdorbagi geese (P<0.05 or P<0.01), and the PRL level was significantly higher than that in Holdorbagi geese (P<0.05).③In terms of gene expression, the expressions of ENO1 and MIS genes of Yili geese were significantly lower than those of Hortobágy geese (P<0.05 or P<0.01); in the pituitary gland, the expression of MIS, ENO1 and BMP6 genes in Yili geese was significantly or extremely significantly lower than that in Holdorbagi geese (P< 0.05 or P<0.01); in the ovary, the expression of ENO1 and MIS genes in Yili geese was significantly or extremely significantly lower than that in Holdorbagi geese (P<0.05 or P<0.01); in the fallopian tube, the expression of ENO1 and EP300 genes in Yili geese was significantly or extremely significantly lower than that in Holdorbagi geese (P<0.05 or P<0.01), and there were no significant differences in the expression of other genes (P > 0.05).【Conclusion】 Fertilization rate and hatching rate of fertilized eggs in Yili geese are lower than those in Holdorbagi geese, and the serum reproductive hormones are lower than those in Holdorbagi geese except that the PRL level was higher than that in Holdorbagi geese.The differences in gene expression of BMP2, BMP6, MIS, ENO1 and EP300 may affect the differences in follicular development between geese of different breeds, thus regulating the reproductive performance of geese.

Key words: Yili goose; Hortobágy geese; reproductive performance; serum hormone; gene expression

摘要：

【目的】研究伊犁鹅和霍尔多巴吉鹅在生产性能、孵化性能、血清生殖激素水平和不同组织中与鹅繁殖性能相关的基因表达量之间存在的差异,为伊犁鹅杂交育种改良提供理论支撑。【方法】选取健康状况良好、饲养条件一致、处于产蛋期的2岁伊犁鹅和霍尔多巴吉鹅各120只,进行产蛋量、血清激素水平及BMP2、BMP6、MIS、ENO1及EP300基因表达量的测定。【结果】①伊犁鹅的产蛋率、日产蛋量、平均日采食量、平均蛋重、蛋形均显著或极显著低于霍尔多巴吉鹅(P<0.05或P<0.01),料蛋比极显著高于霍尔多巴吉鹅(P<0.01);在孵化性能方面,伊犁鹅的受精率和受精蛋孵化率均显著或极显著低于霍尔多巴吉鹅(P<0.05或P<0.01),其余指标均无显著差异。②伊犁鹅血清GnRH、LH、FSH、E₂和P₄水平均显著或极显著低于霍尔多巴吉鹅(P<0.05或P<0.01),PRL水平显著高于霍尔多巴吉鹅(P<0.05)。③在下丘脑中,伊犁鹅的ENO1和MIS基因的表达量均显著或极显著低于霍尔多巴吉鹅(P<0.05或P<0.01);在垂体中,伊犁鹅的MIS、ENO1和BMP6基因的表达量均显著或极显著低于霍尔多巴吉鹅(P<0.05或P<0.01);在卵巢中,伊犁鹅的ENO1和MIS基因的表达量显著或极显著低于霍尔多巴吉鹅(P<0.05或P<0.01);在输卵管中,伊犁鹅的ENO1和EP300基因显著或极显著低于霍尔多巴吉鹅(P<0.05或P<0.01),其余基因表达量间均无显著差异(P>0.05)。【结论】伊犁鹅的产蛋率、受精率和受精蛋孵化率等均低于霍尔多巴吉鹅,血清生殖激素除PRL水平高于霍尔多巴吉鹅,其余激素均低于霍尔多巴吉鹅。BMP2、BMP6、MIS、ENO1、EP300基因表达量之间的差异可能影响不同品种鹅之间的卵泡发育差异,继而调控鹅的繁殖性能。

关键词: 伊犁鹅, 霍尔多巴吉鹅, 繁殖性能, 血清激素, 基因表达量

CLC Number:

S835

TANG Bihui, ZHANG Lihua, LI Haiying, ZHANG Chong, JIANG Tinghao, ZHAO Xiaoyu, JIANG Teng, DING Yawen, WU Yingping, ZHAO Quanzhuang. A comparative study on reproductive performance, serum hormone levels and gene expression between Yili goose and hortobágy goose[J]. Xinjiang Agricultural Sciences, 2023, 60(5): 1271-1280.

唐碧徽, 张俐华, 李海英, 张冲, 蒋廷浩, 赵晓钰, 蒋腾, 丁雅文, 吴盈萍, 赵全庄. 伊犁鹅和霍尔多巴吉鹅在繁殖性能、血清激素水平和基因表达量的比较[J]. 新疆农业科学, 2023, 60(5): 1271-1280.

Figures/Tables 6

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饲粮组成 Diet composition	含量,% Contents	营养水平^② Nutrient levels	含量,% Contents
玉米 Corn	58.00	代谢能,MJ/kg	11.03
豆粕 Soybean meal	20.00	粗蛋白质,%	15.16
苜蓿草粉 Alfalfa meal	12.50	粗纤维,%	5.79
大豆油 Soybean Oil	2.10	蛋氨酸+半胱氨酸,%	0.57
石粉 Limestone	4.40	蛋氨酸,%	0.32
预混料 Limestone^①	3.00	赖氨酸,%	0.84
总计 Total	100.00	钙,%	2.39
		磷,%	0.42
		苏氨酸,%	0.57
		色氨酸,%	0.18

饲粮组成 Diet composition	含量,% Contents	营养水平^② Nutrient levels	含量,% Contents
玉米 Corn	58.00	代谢能,MJ/kg	11.03
豆粕 Soybean meal	20.00	粗蛋白质,%	15.16
苜蓿草粉 Alfalfa meal	12.50	粗纤维,%	5.79
大豆油 Soybean Oil	2.10	蛋氨酸+半胱氨酸,%	0.57
石粉 Limestone	4.40	蛋氨酸,%	0.32
预混料 Limestone^①	3.00	赖氨酸,%	0.84
总计 Total	100.00	钙,%	2.39
		磷,%	0.42
		苏氨酸,%	0.57
		色氨酸,%	0.18

基因 Gene	引物序列(5'→3') Primer sequence (5'→3')	片段大小 Fragment size (bp)
GAPDH	F:TGATGCTCCCATGTTCGTGATG; R:GTGATGGCATGGACAGTGGT	168
BMP2	F:GTGGGGTGGAATGACTGGAT; R:TGGAAAAGGACATTCCCCGT	75
BMP6	F:CTTCCTCAACGACGCTGACA; R:GCTGGTGAGGCGTGAATTCC	77
MIS	F:CGATGCAGCCACCAAAATCA; R:TTTTACCCCAAACGTCCCAATG	184
ENO1	F:GTCACCGCTCTGGAGAAACA; R:AGTTGATGCGAGGGTTCCTG	195
EP300	F:TGCAGCACTGAAGGATCGG; R:CCTGGTTCTTCGCTTTTCCTC	163

基因 Gene	引物序列(5'→3') Primer sequence (5'→3')	片段大小 Fragment size (bp)
GAPDH	F:TGATGCTCCCATGTTCGTGATG; R:GTGATGGCATGGACAGTGGT	168
BMP2	F:GTGGGGTGGAATGACTGGAT; R:TGGAAAAGGACATTCCCCGT	75
BMP6	F:CTTCCTCAACGACGCTGACA; R:GCTGGTGAGGCGTGAATTCC	77
MIS	F:CGATGCAGCCACCAAAATCA; R:TTTTACCCCAAACGTCCCAATG	184
ENO1	F:GTCACCGCTCTGGAGAAACA; R:AGTTGATGCGAGGGTTCCTG	195
EP300	F:TGCAGCACTGAAGGATCGG; R:CCTGGTTCTTCGCTTTTCCTC	163

项目 Item	伊犁鹅 Yili geese	霍尔多巴吉鹅 Hortobágy geese	P值 P-value
产蛋率Egg production rate(%)	16.28±6.48	30.03±5.85^**	0.000
日产蛋量Daily egg production(g/d)	95.20±23.66	174.37±34.28^**	0.000
商品蛋合格率Qualified rate of commercial eggs(%)	97.51±4.57	98.15±2.46	0.551
平均日采食量Average daily feed intake(g/只·d)	182.65±9.50	231.52±5.79^**	0.000
料蛋比Feed/egg ratio	7.36±2.64^**	5.36±1.60	0.007
平均蛋重Mean egg weight(g/枚)	137.36±10.27	158.72±6.58^**	0.000
蛋形指数Egg-shape index	1.48±0.04	1.52±0.03^**	0.001
受精率Fertilization rate(%)	75.74±6.29	93.38±3.99^**	0.000
受精蛋孵化率Fertilized egg hatching rate(%)	75.59±5.88	86.23±8.15^*	0.010
健雏率Healthy young rate(%)	80.74±15.55	81.11±7.89	0.953