Effects of 2,4-epibrassinolide on growth and related genes expression of watermelon seedlings under drought Stress

doi:10.6048/j.issn.1001-4330.2024.03.011

Abstract

Abstract:

【Objective】 To reveal the effects of exogenous 2,4-epibrassinolide (EBR) on the growth and related gene expression of watermelon seedlings under drought stress,so as to provide a theoretical basis for the study of watermelon drought resistance.【Methods】 Watermelon variety Qihong 1 was taken as the experimental material by using 15% polyethylene glycol (PEG6000) to simulate drought stress,and then,three treatments were set up: CK (clear water),PEG and PEG + EBR,in which the concentration of EBR was 0.01 mg/L.Finally,the biomass,photosynthesis index,chlorophyll content,antioxidant enzyme activity and expression of related genes of watermelon seedlings after different treatments were detected.【Results】 Spraying exogenous EBR could alleviate the inhibition and damage of drought stress on the growth and development of watermelon seedlings and also activate EBR signal transduction pathway and improve the drought tolerance of watermelon seedlings by increasing the expression of response genes.Compared with PEG stress,the plant height,root length,aboveground fresh weight and underground fresh weight of watermelon seedlings sprayed with exogenous EBR increased by 2.41%,36.76%,1.88% and 3.42% respectively.Moreover,it also enhances various indicators of photosynthesis and the activities of antioxidant enzymes (POD and SOD).The expression of key response genes BRI1,BIN2,BES1 and DWF4 of Brassinolide (BRS) signal transduction pathway also changed to varying degrees,and the expression of drought related genes CDSP32 and MYB101 also changed significantly.【Conclusion】 Exogenous EBR can effectively alleviate the damage caused by drought stress to watermelon seedlings,thus improving the drought resistance of watermelon seedlings.

Key words: watermelon seedings; 2,4-epibrassinolide; drought stress; growth index; gene expression

摘要：

【目的】 研究外源2,4-表油菜素内酯(2,4-epibrassinolide,EBR)对干旱胁迫条件下西瓜幼苗生长以及相关基因表达的影响,为西瓜抗旱研究提供理论基础。【方法】 以西瓜品种齐红1号为材料,利用15%聚乙二醇(PEG6000)模拟干旱胁迫,设置3个处理CK(清水)、PEG和PEG+EBR,其中EBR的浓度为0.01 mg/L。检测不同处理后西瓜幼苗的生物量、光合作用指标、叶绿素含量、抗氧化酶活性以及相关基因的表达量。【结果】 喷施外源EBR可以缓解干旱胁迫对西瓜幼苗生长发育的抑制和损害程度,还可以通过增加应答基因的表达量,激活EBR信号转导途径,提高西瓜幼苗的耐旱性。喷施外源EBR西瓜幼苗株高、根长、地上鲜重、地下鲜重较PEG胁迫分别增加了2.41%、36.76%、1.88%和3.42%;而且也增强光合作用各项指标,抗氧化酶(POD和SOD)活性。油菜素内酯(BRs)信号转导途径关键应答基因BRI1、BIN2 、BES1 和DWF4的表达量也呈现不同程度变化,抗旱相关基因CDSP32和MYB101的表达量也有显著变化。【结论】 外源EBR能够有效缓解干旱胁迫对西瓜幼苗造成的损害,从而提高西瓜幼苗的抗旱性。

关键词: 西瓜幼苗, 2,4-表油菜素内酯, 干旱胁迫, 生长指标, 基因表达

CLC Number:

S182
S651

ZHANG Wei, YANG Guohui, YU Hui. Effects of 2,4-epibrassinolide on growth and related genes expression of watermelon seedlings under drought Stress[J]. Xinjiang Agricultural Sciences, 2024, 61(3): 615-622.

张伟, 杨国慧, 于辉. 2,4-表油菜素内酯对干旱胁迫下西瓜幼苗生长及相关基因表达的影响[J]. 新疆农业科学, 2024, 61(3): 615-622.

Figures/Tables 5

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西瓜基因 Watermelon gene	同源基因名称 Homologous gene name	引物序列(5'-3') Primer sequence(5'-3')	基因功能 Gene function
Cla97C05G101410	BRI1	F:ATCTCCTCTCCCACCTTCCT R:CTTGCTCGGTAATCTCGCAC	BRI1激酶抑制剂
Cla97C07G136530	BIN2	F:GGTTGCCATTGGTGTTGTCT R:CCGACTGACCAAAGCTAACG	DNA结合蛋白
Cla97C08G158860	BES1	F:GACCTCAGCTCCATACTCCC R:GGTCAGCAGGTTTCACAAGG	BR信号转导
Cla97C09G163730	DWF4	F:AATTGTACCCGCGTATTGGC R:TACCCTGCTTCCCACCAAAA	油菜素类固醇生物合成
Cla97C08G157590	CDSP32	F:TCCGACGGTGATAAAGCTGT R:TCTCCTTTACCGGACCCAAC	硫氧还蛋白
Cla97C10G189540	MYB101	F:ACCACGCCGCAGTTAATTTT R:CTGGGTTGATGAAGGGAGGT	MYB相关转录因子
β-Actin		F:GTCGTACAACAGGTATTGTG R:AAGGTCCAGACGGAGGATAG	内参基因

西瓜基因 Watermelon gene	同源基因名称 Homologous gene name	引物序列(5'-3') Primer sequence(5'-3')	基因功能 Gene function
Cla97C05G101410	BRI1	F:ATCTCCTCTCCCACCTTCCT R:CTTGCTCGGTAATCTCGCAC	BRI1激酶抑制剂
Cla97C07G136530	BIN2	F:GGTTGCCATTGGTGTTGTCT R:CCGACTGACCAAAGCTAACG	DNA结合蛋白
Cla97C08G158860	BES1	F:GACCTCAGCTCCATACTCCC R:GGTCAGCAGGTTTCACAAGG	BR信号转导
Cla97C09G163730	DWF4	F:AATTGTACCCGCGTATTGGC R:TACCCTGCTTCCCACCAAAA	油菜素类固醇生物合成
Cla97C08G157590	CDSP32	F:TCCGACGGTGATAAAGCTGT R:TCTCCTTTACCGGACCCAAC	硫氧还蛋白
Cla97C10G189540	MYB101	F:ACCACGCCGCAGTTAATTTT R:CTGGGTTGATGAAGGGAGGT	MYB相关转录因子
β-Actin		F:GTCGTACAACAGGTATTGTG R:AAGGTCCAGACGGAGGATAG	内参基因

处理 Treatment	株高 Plant height (cm)	根长 Root length (cm)	地上鲜重 Fresh weight above ground (mg)	地下鲜重 Fresh weigh underground (mg)	地上干重 Dry weight above ground (mg)	地下干重 Dry weight underground (mg)	根冠比 Root-shoot ratio
CK	19.67±0.10^c	16.49±0.07^c	8.55±0.02^b	3.64±0.17^b	3.52±0.06^b	0.34±0.01^b	0.09±0.04^a
PEG	18.49±0.05^a	11.27±0.03^a	7.27±0.03^a	3.02±0.02^a	2.78±0.09^a	0.26±0.01^a	0.10±0.03^b
PEG+EBR	18.94±0.06^b	15.41±0.22^b	7.40±0.14^a	3.12±0.02^a	2.73±0.02^a	0.28±0.01^a	0.10±0.01^b

处理 Treatment	株高 Plant height (cm)	根长 Root length (cm)	地上鲜重 Fresh weight above ground (mg)	地下鲜重 Fresh weigh underground (mg)	地上干重 Dry weight above ground (mg)	地下干重 Dry weight underground (mg)	根冠比 Root-shoot ratio
CK	19.67±0.10^c	16.49±0.07^c	8.55±0.02^b	3.64±0.17^b	3.52±0.06^b	0.34±0.01^b	0.09±0.04^a
PEG	18.49±0.05^a	11.27±0.03^a	7.27±0.03^a	3.02±0.02^a	2.78±0.09^a	0.26±0.01^a	0.10±0.03^b
PEG+EBR	18.94±0.06^b	15.41±0.22^b	7.40±0.14^a	3.12±0.02^a	2.73±0.02^a	0.28±0.01^a	0.10±0.01^b

处理 Treatment	净光合作用速率P_n Photosynthetic rate (μmol CO₂/(m²·s))	气孔导度G_s Conductance to H₂O (mmol/(m²·s))	胞间CO₂浓度C_i Intercellular CO₂ concentration(μL/L)	蒸腾速率T_r Transpiration (mmol H₂O/(m²·s))	叶绿素含量 Chlorophyll content (mg/g)
CK	20.80±0.36^c	0.62±0.02^c	458.00±8.18^a	6.59±0.17^b	18.23±0.12^a
PEG	13.03±0.21^a	0.27±0.03^a	504.00±7.21^c	4.96±0.18^a	21.50±0.06^b
PEG+EBR	18.90±0.20^b	0.34±0.01^b	486.00±9.17^b	5.05±0.05^a	20.58±0.34^b