Abstract:

【Objective】To excavate the genes related to drought resistance in the hope of providing theoretical support for analyzing the molecular drought resistance mechanism of barley and guiding the drought resistance breeding of barley.【Methods】Taking New beer No.6 as the test material, we applied transcriptome sequencing RNA-seq technology to sequence the inverted bilobed leaves of barley seedlings before and after drought stress, and real-time fluorescence quantitative RT-PCR was used to verify the functional genes.【Results】(1) In this study, 3835 differentially expressed genes were detected in the inverted bilobed of New beer 6 before and after drought stress by using RNA-seq, mainly genes encoded by ABC transporter proteins, ribosomal proteins, transcription factors, dehydrins, peroxidases, protein phosphatases, etc.The results showed that DEG was mainly enriched in starch and sucrose metabolism, transporter proteins, plant hormone signaling transduction, and protein phosphatases.(2) By KEGG metabolic pathway enrichment analysis, DEGs were mainly enriched in starch and sucrose metabolism, transporter proteins, phytohormone signaling, chaperones and folding catalysts, aminosugar and ribosugar metabolism, phenylalanine metabolism, taurine and hyposulphite metabolism, peroxisomes and other pathways.【Conclusion】Significant differences are shown in gene expression before and after drought stress in barley.The results of this study have laid the foundation for the excavation of key drought-resistant genes and for further analysis of drought-resistant mechanisms in barley(1592 up-regulated genes and 2243 down-regulated genes).

Key words: barley; drought stress; transcriptome; gene mining

摘要：

【目的】挖掘与分析大麦苗期抗旱相关基因,为研究大麦分子抗旱机制和选育抗旱大麦品种提供理论支撑。【方法】以啤酒大麦品种新啤6号为材料,应用转录组测序RNA-seq技术对干旱胁迫前后大麦苗期倒二叶叶片进行转录组测序,并采用实时荧光定量 RT-PCR 方法进行功能基因验证。【结果】(1)干旱胁迫前后新啤6号倒二叶中3 835个差异表达基因,主要为编码ABC转运蛋白、核糖体蛋白、转录因子、脱水素、过氧化物酶、蛋白质磷酸酶等的基因。(2)DEG主要富集在淀粉和蔗糖代谢、转运蛋白、植物激素信号转导、伴侣和折叠催化剂、氨基糖和核苷酸糖的代谢、苯丙氨酸代谢、牛磺酸和次牛磺酸代谢、过氧化物酶体等途径。【结论】大麦干旱胁迫前后基因表达差异显著(其中上调基因1 592个,下调基因2 243个)。

关键词: 大麦, 干旱胁迫, 转录组, 基因挖掘