Effect of Exogenous ALA on Fruit Quality of Crimson Seedless Grape

doi:10.6048/j.issn.1001-4330.2019.05.004

Abstract

Abstract: 【Objective】 To clarify the effects of fruit quality of different concentrations of exogenous ALA on the fruit quality Crimson seedless grapes.【Method】In this experiment, Krissom seedless grape was used as the test material to the effects of different concentrations of exogenous ALA on glycolic acid content, longitudinal and transverse diameter, anthocyanin content and PAL enzyme activity of Kerrison seedless grape were studied.【Results】ALA treatment with different concentrations significantly increased the longitudinal and transverse diameter of fruits, the chlorophyll relative content increased by 12.03%, and the average ratio of sugar to acid in fruit was 106.21% higher than that in the control. The accumulation of anthocyanin and the activity of the associated PAL increased significantly, with an average increase of 194.71% and 82.35% respectively. Anthocyanin was positively correlated with PAL enzyme activity and soluble sugar content in Crimson peel, and was negatively correlated with titratable acid content. 【Conclusion】 Compared with the control, the best promotion effect was 100 mg/L of ALA. Exogenous ALA treatment can improve the fruit size and sugar-acid ratio of Crimson seedless grape and promote fruit flavor and color to a certain extent

Key words: Crimson seedless grape; 5-aminolevulinic acid (ALA); quality; anthocyanin

摘要： 【目的】研究不同浓度外源ALA对克瑞森无核葡萄的果实品质效果。【方法】以露地克瑞森无核葡萄为材料,研究不同浓度的外源ALA对克瑞森葡萄果实糖酸含量、果实纵横径、花青素含量及PAL酶活性的影响。【结果】不同浓度的ALA处理能明显增加果实的纵、横径;叶片内叶绿素相对含量提高12.03%;果实糖酸比平均值与对照相比增幅可达106.21%;果皮花青素的积累量及其合成相关PAL的活性显著升高,与对照相比,平均增幅分别达到194.71%、82.35%;克瑞森果皮中花青素与其合成相关的PAL酶活性、果肉中可溶性糖的含量具有极显著正相关关系,与果肉中可滴定酸的含量呈明显负相关关系。【结论】以100 mg/L 的ALA的促进效果最好,外源ALA处理可提高克瑞森无核葡萄果实大小及糖酸比,促进果实风味,并在一定程度上促进其着色。

关键词: 克瑞森无核葡萄, 5-氨基乙酰丙酸(ALA), 品质, 花青素

CLC Number:

S663.1

LIU Jing-jing, SUN Jun-li, ZHAO Bao-long, ZHANG Meng-yan, ZHANG Shuai. Effect of Exogenous ALA on Fruit Quality of Crimson Seedless Grape[J]. Xinjiang Agricultural Sciences, 2019, 56(5): 817-825.

刘晶晶, 孙军利, 赵宝龙, 张梦燕, 张帅. 外源ALA对克瑞森无核葡萄果实品质的影响[J]. 新疆农业科学, 2019, 56(5): 817-825.

References 35

[1]	Stenbaek, A., & Jensen, P. E. (1995). Redox regulation of chlorophyll biosynthesis. Phytochemistry, 7(7):1,039-1,057.
[2]	赵艳艳,胡晓辉,邹志荣,等.不同浓度5-氨基乙酰丙酸(ALA)浸种对NaCl胁迫下番茄种子发芽率及芽苗生长的影响[J].生态学报, 2013, 33(1) : 62-70.ZHAO Yan-yan, HU Xiao-hui, ZOU Zhi-rong, et al. (2013). Effects of seed soaking with different concentrations of 5-aminolevulinic acid on the germination of tomato ( Solanumlycopersicum) seeds under Na Cl stress [J]. Acta Ecologica Sinica, 33(1): 62-70. (in Chinese)
[3]	汪良驹,姜卫兵,黄保健,等.5-氨基乙酰丙酸对弱光下甜瓜幼苗光合作用和抗冷性的促进效应[J].园艺学报, 2004, 31(3): 312-316.WANG Liang-ju, JIANG Wei-bing, HUANG Bao-jian, et al.(2004). Promotion of Photosynthesis by 5-Aminolevulinic Acid (ALA) during and af-ter Chilling Stress in Melon Seedlings Grown under Low Light Condition [J]. Acta Horticulturae Sinica, 31(3): 312-316. (in Chinese)
[4]	徐晓洁, 邹志荣, 乔飞, 等. ALA对NaCl胁迫下不同品种番茄植株光合作用、保护酶活性及果实产量的影响[J].干旱地区农业研究, 2008, 26(4): 131-135.XU Xiao-jie, ZOU Zhi-rong, QIAO Fei, et al. (2008). Effects of ALA on tomato in photosynthesis, activity of protectiveenzymes and yield under NaCl stress [J]. Agricultural Research in the Arid Areas. 26(4): 131-135. (in Chinese)
[5]	孙永平. 5-氨基乙酰丙酸(ALA)提高逆境条件下西瓜幼苗叶片光合与光抑制保护机理研究[D]. 南京:南京农业大学博士论文, 2009.SUN Yong-ping. (2009). Study on the mechanism of promotion by 5-aminolevulinic acid (ALA) on photosynthesis and photoinhibition of watermelon leaves under stresses [D]. PhD Dissertation. Nanjing Agricultural University, Nanjing. (in Chinese)
[6]	刘玉梅, 艾希珍, 于贤昌. 5-氨基乙酰丙酸对亚适宜温光条件下黄瓜幼苗光合特性的影响[J].园艺学报, 2010, 37(1): 65-71.LIU Yu-mei, AI Xi-zhen, YU Xian-chang. (2010). Effects of ALA on photosynthesis of cucumber seedlings under suboptimal temperature and light intensity[J]. Acta Horticulturae Sinica, 37(1):65-71.(in Chinese)
[7]	王魏, 邹志荣, 乔飞, 等. 外源ALA对NaCl胁迫下菠菜生理特性的影响[J].西北农业学报, 2008, 17(1) : 137-141, 156.WANG Wei, ZOU Zhi-rong, QIAO Fei, et al. (2008). Effects of exogenous ALA on physiological characteristicsof spinach under NaCl stress[J]. Acta Agriculturae Boreali-occidentalis Sinica, 17(1) :137-141, 156. (in Chinese)
[8]	刘卫琴, 康琅, 汪良驹. ALA对草莓光合作用的影响及其与抗氧化酶的关系[J]. 西北植物学报, 2006, 26(1): 57-62.LIU Wei-qin, KANG Lang, WANG Liang-ju. (2006). Effects on strawberry photosynthesis and relationsto anti-oxidant enzymes of ALA[J]. Acta Bot. Boreal.-Occident.Sin, 26(1): 57-62. (in Chinese)
[9]	张帆,郁继华,颉建明, 等.外源ALA和Spd对低温弱光下辣椒幼苗光合作用及抗氧化系统的影响[J]. 中国农业科学, 2013, 46(11):2 298-2 306.ZHANG Fan, YU Ji-hua, JIE Jian-ming, et al. (2013). Influence of exogenous ALA and spd on photosynthesis and antioxidant system of low temperature and poor light affected pepper seedlings[J]. Scientia Agricultura Sinica, 46(11): 2,298-2,306. (in Chinese)
[10]	赵宝龙,刘鹏,张祥, 等.5-氨基乙酰丙酸对盐胁迫下葡萄叶片光合特性及生长特性的影响[J].北方园艺,2016,(14):1-6.ZHAO Bao-long, LIU Peng, ZHANG Xiang, et al. (2016). Effect of 5-aminoleulinic on photosynthetic characteristics and growth characteristics of grape leaves under salt stress[J]. (14):1-6. (in Chinese)
[11]	张春平,何平,袁凤刚, 等.外源5-氨基乙酰丙酸对干旱胁迫下甘草种子萌发及幼苗生理特性的影响[J].西北植物学报,2011,31(8):1 603-1 610.ZHANG Chun-ping, HE Ping, YUAN Feng-gang, et al. (2011). Effect of exogenous ALA on seed germination and physiological characteristics of glycyrrhiza uralensis seedlings under drought stress[J]. Acta Bot.Boreal.-Occident.Sin, 31(8):1,603-1,610. (in Chinese)
[12]	徐铭. ALA在设施园艺蔬菜上应用效果的研究[D].杨凌:西北农林科技大学,2008.XU Ming. (2008). Study on applying ALA in greenhouse vegetable [D]. PhD Dissertation Northwest A&F University,Yangling. (in Chinese)
[13]	王中华,汤国辉,李志强, 等.5-氨基乙酰丙酸和金雀异黄素促进苹果果皮花青素形成的效应[J].园艺学报,2006,33(5):1 055-1 058.WANG Zhong-hua, TANG Guo-hui, LI Zhi-qiang, et al. (2006). Promotion of 5-am inolevulinic acid and genistein on anthocyanin accumula-tion in apples[J]. Acta Horticulturae Sinica, 33(5): 1,055-1,058 . (in Chinese)
[14]	高晶晶, 段春慧,冯新新, 等.ALA提高苹果叶片光合性能与果实品质的效应[J].果树学报,2013,30(6):944-951.GAO Jing-jing, DUAN Chun-hui,FENG Xin-xin, et al. (2013). Effects of 5-aminolevulinic acid (ALA) on leaf photosynthesis and fruitquality of apples [J]. Journal of Fruit Science, 30(6): 944-951.(in Chinese)
[15]	谢荔. ALA诱导葡萄和苹果果皮花青素积累机理的研究[D]. 南京: 南京农业大学博士论文,2012.XIE Li. (2012). Study on promotion of 5-aminolevulinic acid on fruit coloration in grape and apple and its mechanism in inducing anthocyanin accumulation in skin [D]. PhD Dissertation. Nanjing Agricultural University, Nanjing. (in Chinese)
[16]	汪良驹,王中华,李志强,等.5-氨基乙酰丙酸促进苹果果实着色的效应[J].果树学报,2004,30(6):512-515.WANG Liang-ju, WANG Zhong-hua, LI Zhi-qiang, et al.(2004). Effect of 5-aminolevulinic acid on enhancing apple fruit coloration[J]. Journal of Fruit Science, 30(6): 512-515.(in Chinese)
[17]	郭磊,蔡志翔,张斌斌, 等.5-氨基乙酰丙酸促进桃果皮提前着色机制研究[J].园艺学报,2013,40(6): 1 043-1 050.GUO Lei, CAI Zhi-xiang, ZHANG Bin-bin, et al. (2013). The mechanism analysis of anthocyanin accumulation in peach accelerated by ALA[J]. Acta Horticulturae Sinica,40(6): 1,043-1,050. (in Chinese)
[18]	肖长城,张绍铃,胡红菊, 等.套袋和外源5-氨基乙酰丙酸处理对'云红梨2号'果皮着色的影响[J].南京农业大学学报,2012,35(6):25-29.XIAO Chang-cheng, ZHANG Shao-ling, HU Hong-ju, et al. (2012). Effects of bagging and exogenous 5-aminolevulinic acidtreatment on coloration of 'Yunhongli 2'[J]. Journal of Nanjing Agricultural University, 35(6) :25-29.(in Chinese)
[19]	李合生.植物生理生化实验原理与技术[M].北京:高等教育出版社,2000.LI He-sheng. (2000). Experimental principles and techniques of plant physiology and biochemistry [M]. Beijing: Higher Education Press.(in Chinese)
[20]	沈德绪.果树育种技术[D].北京:农业出版社,1992.SHENG De-xu. (1992). Fruit tree breeding technology [M]. Beijing: China Agriculture Press.(in Chinese)
[21]	Lister, C. E., Lancaster, J. E., & Walker, J. R. L. (2015). Developmental changes in enzymes of flavonoid biosynthesis in the skins of red and green apple cultivars. Journal of the Science of Food & Agriculture, 71(3):313-320.
[22]	仝月澳, 周厚基. 果树营养诊断法[M]. 北京: 农业出版社, 1982.TONG Yue-ao, ZHOU Hou-ji. (1982). Fruit tree nutrition diagnosis method [M]. Beijing: China Agriculture Press. (in Chinese)
[23]	沈苗苗. 红肉脐橙输导组织结构及输导速率对果实品质的影响[D].武汉:华中农业大学博士论文,2011.SHEN Miao-miao.(2011). The impact of conduit structure and transporting rate on fruit quality of cara cara navel orange [D]. PhD Dissertation. Huazhong Agricultural University, Wuhan. (in Chinese)
[24]	姚素梅,茹振钢,刘明久, 等.5-氨基乙酰丙酸(ALA)对冬小麦花后干物质生产和旗叶衰老的影响[J].应用生态学报,2011,22(2):383-388.YAO Su-mei, RU Zhen-gang, LIU Ming-jiu, et al. (2011). Effects of 5-aminolevulinic acid on winter wheat dry matter accumulation after anthesis and flag leaf senescence [J]. Chinese Journal of Applied Ecology, 22(2):383-388.(in Chinese)
[25]	Elliott, T. (1993). Transport of 5-aminolevunilic acid by the dipeptide permease in S almonella typhimurium. Journal of Bacteriology, 175(2):325-31.
[26]	Tanaka, Y., Tanaka, A., & Tsuji, H. (1993). Effects of 5-aminolevulinic acid on the accumulation of chlorophyll b and apoproteins of the light-harvesting chlorophyll a/b-protein complex of photosystem ii [in cucumis sativus]. Plant & Cell Physiology, 34(3): 465-472.
[27]	Sasaki, K., Marquez, F. J., Nishio, N., & Nagai, S. (1995). Promotive effect of 5-aminolevulinic acid on the growth and photosynthesis of spirulina platensis. Journal of Fermentation & Bioengineering, 79(5):453-457.
[28]	康博文,李文华,刘建军,等.ALA对红掌叶片光合作用及叶绿素荧光参数的影响[J].西北农林科技大学学报(自然科学版),2009,37(4):97-102.KANG Bo-wen, LI Wen-hua, LIU Jian-jun, et al. (2009). Effects of ALA treatment on photosynthetic and chlorophyII fluorescence dynamics of anthurium andraeanum[J]. Journal of Northwest A&F University (Nat. Sci. Ed.). 37(4): 97-102.(in Chinese)
[29]	Hotta, Y., Tanaka, T., Takaoka, H., Takeuchi, Y., & Konnai, M. (1997). Promotive effects of 5-aminolevulinic acid on the yield of several crops. Plant Growth Regulation, 22(2):109-114.
[30]	Hecke, K. , Herbinger, K. , Veberic, R. , Trobec, M. , & Grill, D. (2006). Sugar-, acid- and phenol contents in apple cultivars from organic and integrated fruit cultivation. European Journal of Clinical Nutrition, 60(9): 1,136-1,140.
[31]	Petkovsek, M. M., Stampar, F., & Veberic, R. (2007). Parameters of inner quality of the apple scab resistant and susceptible apple cultivars (malus domestica borkh.). Scientia Horticulturae, 114(1): 0-44.
[32]	Watanabe, K., Nishihara, E., Watanabe, S., Tanaka, T., Takahashi, K., & Takeuchi, Y. (2006). Enhancement of growth and fruit maturity in 2-year-old grapevines cv. Delaware by 5-aminolevulinic acid. Plant Growth Regulation, 49(1): 35-42.
[33]	Koukol. J., Conn, E. E. (1961). The metabolism of aromatic compounds in higher plants.IV. Purification and properties of the phenylalanine deaminase of Herdeum vulagare. Journal of Biological Chemistry, 236:2,692-2,698.
[34]	李莉,赵越,马君兰.苯丙氨酸代谢途径关键酶: PAL、C4H、4CL研究新进展[J]. 生物信息学, 2006, 4(5) :187-189.LI li, ZHAO Yue, MA Jun- lan. (2006). Recent progress on key enzymes: PAL、C4H、4CL of phenylalanine metabolism pathway [J]. China Journal of Bioinformatics, 4(5):187-189. (in Chinese)
[35]	马俊彦,杨汝德,敖利刚.植物苯丙氨酸解氨酶的生物学研究进展[J].现代食品科技,2007,(7):71-74.MA Jun-yan, YANG Ru-de, AO Li-gang. (2007). Progress in biological research of phenylanlanine ammonialyase [J]. Modern Food Science and Technology. (7): 71-74. (in Chinese)