Effects of simulated cold chain transport on cell membrane lipid peroxidation and quality of apricot fruit during storage

doi:10.6048/j.issn.1001-4330.2023.09.014

Abstract

Abstract:

【Objective】 To investigate the effects of near freezing temperature and cold chain transportation on storage quality and cell membrane lipid peroxidation of postharvest apricot fruits.【Methods】 Xinjiang 'Saimaiti apricot' was used as material, the vibration platform and cold storage were used to simulate the vibration environment of near freezing temperature cold chain transportation.The changes of quality and cell membrane lipid peroxidation of apricot fruits during storage after vibration treatment for 8 hours at different transportation temperatures of 1-2℃ (CK₁), 4-6℃ (CK₂) and near freezing temperature (-1.1--1.6℃) were studied.【Results】 The results showed that the near freezing temperature (-1.1--1.6℃) transportation treatment of apricot fruits effectively slowed down the increase of cell membrane permeability, malondialdehyde content accumulation and hydrogen peroxide (H₂O₂) content, as well as the increase of polyphenol oxidase (PPO), Lipoxygenase (LOX) and catalase (CAT) activities and peroxidase (POD) activities, and delayed the decline of hardness, ascorbic acid and chlorophyll content of apricot fruits.【Conclusion】 Near freezing temperature cold chain transportation can effectively slow down the oxidative damage, aging and softening of cell membrane lipids in apricot fruits.

Key words: apricot fruit; cold chain transport; near freezing temperature storage; quality; membrane lipid peroxidation

摘要：

【目的】研究近冰温冷链运输处理对采后杏果实贮藏品质及细胞膜脂过氧化的影响。【方法】以新疆赛买提杏为材料,利用振动平台及冷藏库来模拟近冰温冷链运输振动环境,分析杏果实分别在不同运输温度1~2℃(CK₁)、4~6℃(CK₂)、近冰温(-1.1~-1.6℃)条件下,振动处理8 h后,贮藏期间品质及细胞膜脂过氧化的变化。【结果】近冰温(-1.1~-1.6℃)运输处理的杏果实较(1~2℃、4~6℃)冷链运输处理的杏果实有效减缓了细胞膜透性增加、丙二醛含量积累和过氧化氢(H₂O₂)含量的增加,及杏果实中多酚氧化酶(PPO)、脂氧合酶(LOX)活性、过氧化氢酶(CAT)活性升高,提高过氧化物酶(POD)活性,延缓了杏果实硬度、抗坏血酸、叶绿素含量下降。【结论】近冰温冷链运输有效减缓了杏果实细胞膜脂的氧化损伤及衰老软化。

关键词: 杏果实, 冷链运输, 近冰温贮藏, 品质, 细胞膜脂过氧化

CLC Number:

S662.2
S37

GONG Duorui, YANG Liling, HAN Jiang, YANG Zhongqiang, LIU Jia, WEN Yu, ZHU Zhanjiang, CUI Kuanbo. Effects of simulated cold chain transport on cell membrane lipid peroxidation and quality of apricot fruit during storage[J]. Xinjiang Agricultural Sciences, 2023, 60(9): 2198-2207.

巩多蕊, 杨莉玲, 韩江, 杨忠强, 刘佳, 文钰, 朱占江, 崔宽波. 模拟冷链处理下不同储藏温度对杏果实贮藏期细胞膜脂过氧化及品质的影响[J]. 新疆农业科学, 2023, 60(9): 2198-2207.

Figures/Tables 14

Fig.1 Front view of the vibration platform Note:1.Motor 1;2. Up and down vibration platform;3. Left and right vibration platform;4. Up and down vibration swing rod;5.Eccentric wheel 2;6. Shake the spring;7. Rack;8.Motor 2

Fig.2 The freezing curve of saimaiti apricot fruits

Fig.3 Effect of different cold chain transport treatments on the firmness of apricot fruits

Fig.4 Effect of different cold chain transport treatments on the TSS of apricot fruits

Fig.5 Effect of different cold-chain transport treatments on the respiratory intensity of apricot fruits

Fig.6 Effect of different cold chain transport treatments on the VC content of apricot fruits

Fig.7 Effect of different cold chain transport treatments on the membrane permeability of apricot fruits cell

Fig.8 Effect of different cold chain transport treatments on MDA content in apricot fruits

Fig.9 Effect of different cold chain transport treatments on the POD activity of apricot fruits

Fig.10 Effect of different cold chain transport treatments on the PPO activity of apricot fruits

Fig.11 Effect of different cold chain transport treatments on the CAT activity of apricot fruits

Fig.12 Effect of different cold chain transport treatments on the LOX activity of apricot fruits

Fig.13 Effect of different cold chain transport treatments on chlorophyll content of apricot fruits

Fig.14 Effect of different cold chain transport treatments on the H2O2 activity of apricot fruits

References 36

[1]	杨婷婷, 朱璇, 向玉洁, 等. 采收成熟度对杏果实贮藏品质的影响[J]. 现代食品科技, 2015, 31(7): 277-282.
	YANG Tingting, ZHU Xuan, XIANG Yujie, et al. Effect of harvest maturity on the storage quality of apricot fruit[J]. Modern Food Technology, 2015, 31(7): 277-282.
[2]	Aubert C, Bony P, Chalot G, et al. Changes in physicochemical characteristics and volatile compounds of apricot (Prunus armeniaca L.cv.Bergeron) during storage and post-harvest maturation[J]. Food Chemistry, 2010, 119(4): 1386-1398. DOI URL
[3]	刘志鸣, 万金庆, 王建民. 日本冰温技术发展史略[J]. 制冷与空调(四川), 2005,(3): 70-74,57.
	LIU Zhiming, WAN Jinqing, WANG Jianming. Development history of Japanese ice temperature technology[J]. Refrigeration and Air Conditioning (Sichuan), 2005,(3): 70-74,57.
[4]	熊小辉. 冰温技术在食品贮藏保鲜中的研究[J]. 广西轻工业, 2010, 26(1): 11-12.
	XIONG Xiaohui. Study on ice temperature technology in food storage and preservation[J]. Guangxi Light Industry, 2010, 26(1): 11-12.
[5]	严灿. 草莓采后全程冷链保鲜技术研究[D]. 上海: 上海海洋大学, 2016.
	YAN Can. Research on the whole cold chain preservation technology after strawberry harvest[D]. Shanghai: Shanghai Ocean University, 2016.
[6]	程曦. 振动胁迫对杏果实采后后熟软化影响的研究[D]. 乌鲁木齐: 新疆农业大学, 2015.
	CHENG Xi. Effect of vibration stress on postripening softening of apricot fruit[D]. Urumqi: Xinjiang Agricultural University, 2015.
[7]	杨军, 廖新福, 沙勇龙, 等. 冷链运输对哈密瓜品质及腐烂率的影响[J]. 新疆农业科学, 2011, 48(7): 1277-1282.
	YANG Jun, LIAO Xinfu, SHA Yonglong, et al. Effect of cold-chain transport on the quality and rot rate of cantaloupe[J]. Xinjiang Agricultural Sciences, 2011, 48(7): 1277-1282.
[8]	张潇方. 甜樱桃采后全程冷链保鲜技术研究[D]. 上海: 上海海洋大学, 2017.
	ZHANG Xiaofang. Rresearch on the whole cold chain preservation technology after sweet cherry harvest[D]. Shanghai: Shanghai Ocean University, 2017.
[9]	Zhao H D, Shu C, Fan X G, et al. Near-freezing temperature storage prolongs storage period and improves quality and antioxidant capacity of nectarines[J]. Scientia Horticulturae, 2018, 228: 196-203. DOI URL
[10]	潘俨, 车凤斌, 董成虎, 等. 模拟运输振动对新疆杏呼吸途径和品质的影响[J]. 农业工程学报, 2015, 31(3): 325-331.
	PAN Yan, CHE Fengbin, DONG Chenghu, et al. Effect of simulated transport vibration on the respiratory pathway and quality of apricot in Xinjiang[J]. Transactions of the Chinese Society of Agricultural Engineering, 2015, 31 (3): 325-331.
[11]	曹建康, 姜微波, 赵玉梅. 果蔬采后生理生化实验指导[M]. 北京: 中国轻工业出版社, 2007: 22-115.
	CAO Jiankang, JIANG Weibo, ZHAO Yumei. Guide of physiological and biochemical experiments after fruit and vegetable harvest[M]. Beijing: China Light Industry Press, 2007: 22-115.
[12]	李君兰, 刘志芳, 南勇. 热水处理对近冰温贮藏鲜枣品质提高与膜脂代谢的相关性分析[J]. 食品工业科技, 2022, 43(1): 335-343.
	LI Junlan, LIU Zhifang, NAN Yong. Analysis of quality of fresh jujube and membrane lipid metabolism by hot water treatment[J]. Food Industry Technology, 2022, 43(1): 335-343.
[13]	Kang R Y, Yu Z F. Effect of coating and intermittent warming on enzymes, soluble pectin substances and ascorbic acid of Prunus persica (Cv.Zhonghuashoutao) during refrigerated storage[J]. Food Research International, 2005, 38(3): 331-336. DOI URL
[14]	Chen A Q, Zhao Y. Quantitative evaluation and prediction for preservation quality of cold shocked cucumber based on entropy[J]. Innovative Food Science & Emerging Technologies, 2016, 35: 58-66.
[15]	Cakmak I, Strbac D, Marschner H. Activities of Hydrogen Peroxide-Scavenging Enzymes in Germinating Wheat Seeds[J]. Journal of Experimental Botany, 1993, 44(1): 127-132. DOI URL
[16]	Axelrod B, Cheesbrough T M. Lipoxygenase from Soybeans[J]. Methods in Enzymoloy, 1981, 7: 443-451.
[17]	Zhou B Y, Wang J H, Guo Z F, et al. A simple colorimetric method for determination of hydrogen peroxidein plant tissues[J]. Plant Growth Regulation, 2006, 49(23): 113-118. DOI URL
[18]	蒋宝. 运输振动损伤及其对果实采后生理特性与品质影响的研究进展[J]. 食品与机械, 2016, 32(12): 209-212.
	JIANG Bao. Progress on transport vibration damage and its influence on physiological characteristics and quality after fruit harvest[J]. Food and Machinery, 2016, 32 (12): 209-212.
[19]	卫赛超, 谢晶. 模拟运输时间对芒果低温贮藏过程中生理与品质的影响[J]. 食品科学技术学报, 2020, 38(3): 43-50.
	WEI Saichao, XIE Jing. Effect of simulated transport time on physiology and quality during cryogenic storage of mango[J]. Journal of Food Science and Technology, 2020, 38 (3): 43-50.
[20]	赵晓梅, 江英, 吴玉鹏, 等. 果蔬中VC含量测定方法的研究[J]. 食品科学, 2006,(3): 197-199.
	ZHAO Xiaomei, JIANG Ying, WU Yupeng, et al. Study on VC Measurement of Content in fruits and vegetables[J]. Food Science, 2006,(3): 197-199.
[21]	叶青, 江志波. 酸碱滴定法与碘滴定法测定维生素C[J]. 理化检验(化学分册), 2007,(5): 410,412.
	YE Qing, JIANG Zhibo. Acid-base titration by iodine and vitamin C[J]. Physical and Chemical Inspection (Chemistry), 2007,(5): 410,412.
[22]	张浩, 周会玲, 张晓晓, 等. “金香”猕猴桃果实冷藏最适温度研究[J]. 北方园艺, 2014,(13): 126-129.
	ZHANG Hao, ZHOU Huiling, ZHANG Xiaoxiao, et al. Study on the optimal refrigeration temperature of "Jinxiang" kiwi fruit[J]. Northern Horticulture, 2014,(13): 126-129.
[23]	赵天宏, 孙加伟, 付宇. 逆境胁迫下植物活性氧代谢及外源调控机理的研究进展[J]. 作物杂志, 2008,(3): 10-13.
	ZHAO Tianhong, SUN Jiawei, FU Yu. Progress in the mechanism of metabolism and exogenous regulation of reactive oxygen species in plants under stress stress[J]. Crops, 2008,(3): 10-13.
[24]	侯亚莉. 柿9-脂氧合酶基因在植物衰老和抗逆反应中的功能研究[D]. 杨凌: 西北农林科技大学, 2016.
	HOU Yali. Function of persimmon 9-lipoxygenase gene in plant senescence and antireverse reaction[D]. Yangling: Northwest A & F University, 2016.
[25]	Gechev T, Willekens H, Van M, et al. Different responses of tobacco antioxidant enzymes to light and chilling stress[J]. J Plant Physiol, 2003, 160(5): 509-515. DOI URL
[26]	Sun J, Li L, You X, et al. Phenolics and polysaccharides in major tropical fruits: chemical compositions, analytical methods and bioactivities[J]. Analytical Methods, 2011, 10(3):2212-2220.
[27]	刘军伟, 胡志和, 苏莹. 紫薯中多酚氧化酶活性的研究及褐变控制[J]. 食品科学, 2012, 33(17): 207-211.
	LIU Junwei, HU Zhihe, SU Ying. Study on polyphenol oxidase activity and browning control in purple sweet potato[J]. Food Science, 2012, 33(17): 207-211.
[28]	Quiroga M, Forchetti D, Taleisnik H, et al. Tomato root peroxidase isoenzymes: kinetic studies of the coniferyl alcohol peroxidase activity, immunological properties and role in response to salt stress[J]. Journal of Plant Physiology, 2001, 158(8): 1007-1013. DOI URL
[29]	Pesis E. The role of the anaerobic metabolites, acetaldehyde and ethanol, in fruit ripening, enhancement of fruit quality and fruit deterioration[J]. Postharvest Biology and Technology, 2005, 37(1): 1-19. DOI URL
[30]	尹杰文, 何晓梅, 贾嘉懿, 等. 基于主成分分析的微孔膜包装对延缓黄瓜冷贮后细胞膜脂过氧化及品质劣变研究[J/OL]. 食品与发酵工业: 1-13[2022-04-07].DOI:10.13995/j.cnki.11-1802/ts.029322. DOI
	YIN Jiewen, HE Xiaomei, JIA Jiayi, et al. Study on lipid peroxidation and quality deterioration of cell membrane based on principal component analysis[J/OL]. The Food and Fermentation Industry: 1-13[2022-04-07].DOI:10.13995/j.cnki.11-1802/ts.029322. DOI
[31]	高恩元, 荆华乾. 番茄冷链运输保鲜技术研究[J]. 制冷技术, 2014, 34(5): 49-53.
	GAO Enyuan, JING Huaqian. Research on tomato chain Technology[J]. Refrigeration Technology, 2014, 34(5): 49-53.
[32]	吴琼, 周然. 冷藏温度对振动胁迫下猕猴桃细胞膜脂氧化及活性氧成分的影响[J]. 食品工业科技, 2019, 40(8): 234-240.
	WU Qiong, ZHOU Ran. Effect of refrigeration temperature on membrane lipid oxidation and reactive oxygen species composition of kiwi cells under vibration stress[J]. Food Industry Technology, 2019, 40(8): 234-240.
[33]	YAMANE A. Development of controlled freezing-point storage of food[J]. Nippon Shokuhin Kogyo Gakkaishi, 1982(29): 736-743.
[34]	白国荣. 冰温贮藏对吊干杏采后贮藏品质及生理影响的研究[D]. 石河子: 石河子大学, 2019.
	BAI Guorong. Study on the influence of ice temperature storage on storage quality and physiology of dried apricot[D]. Shihezi: Shihezi University, 2019.
[35]	王善广. 果蔬贮藏冷害研究进展[J]. 保鲜与加工, 2004,(4): 3-5.
	WANG Shanguang. Research progress on cold harm in fruit and vegetable storage[J]. Preservation and Processing, 2004,(4): 3-5.
[36]	Gill S S, Tuteja N. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants[J]. Plant Physiology Biochemistry, 2010, 48(12): 909-930. DOI URL