玉米ZmCPK9基因在非生物胁迫下的表达分析

doi:10.6048/j.issn.1001-4330.2017.09.005

新疆农业科学 ›› 2017, Vol. 54 ›› Issue (9): 1606-1612.DOI: 10.6048/j.issn.1001-4330.2017.09.005

玉米ZmCPK9基因在非生物胁迫下的表达分析

足木热木·吐尔逊, 陈勋基, 陈果, 李建平, 郝晓燕, 黄全生

新疆农业科学院核技术生物技术研究所,乌鲁木齐 830091

收稿日期:2017-06-19 发布日期:2020-07-22
通信作者: 黄全生(1964-),男,新疆乌鲁木齐人,研究员,博士,研究方向为作物耐逆基因资源及分子生物学,(E-mail) hquansheng@126.com
作者简介:足木热木·吐尔逊 (1981-),女,新疆人 ,助理研究员,研究方向为玉米分子生物学,(E-mail) azze128@163.com
基金资助:
国家自然科学基金项目(31260331);新疆农业科学院优秀青年科技人才基金项目(2014026)

Expression Analysis of ZmCDPK9 Gene in Maize under Abiotic Stress

Zumuremu Turxun, CHENG Xun-ji, CHEN Guo, LI Jian-ping, HAO Xiao-yan, HUANG Quan-sheng

Research Institute of Nuclear and Biotechnologies,Xinjiang Academy of Agricultural Sciences, Urumqi 830091,China

Received:2017-06-19 Published:2020-07-22
Correspondence author: HUANG Quan-sheng(1964-),male, Urumqi, Xinjiang, Professor, supervisor, the main research directions for crop adversity molecular biology,(E-mail) hquansheng@126.com
Supported by:
National Natural Science Foundation of China (31260331) and Outstanding Young Scientific Talent Foundation of Xinjaing Academy of Agricultural Sciences.(2014026)

摘要/Abstract

摘要： 【目的】钙依赖蛋白激酶(CPKs)是一类依赖于Ca²⁺的丝氨酸/苏氨酸蛋白激酶,仅在植物和部分低等生物中存在。Ca²⁺作为第二信使,在植物生长发育和抗逆应答过程中起着重要作用。研究玉米ZmCPK9基因在幼苗期不同逆境胁迫下的动态表达,为分析该基因在玉米逆境应答中的功能和机制奠定基础。【方法】利用生物信息学方法,分析ZmCPK9基因序列特征,采用Real-time PCR技术分析ZmCPK9在干旱、低温、盐和ABA诱导下的表达特征。【结果】利用生物信息学方法从玉米基因组中鉴定出CPK基因,命名为ZmCPK9。序列分析表明ZmCPK9 cDNA序列长1 548 bp,编码515个氨基酸。生物信息学分析显示,在蛋白质序列和结构上该基因与其他物种CPK基因一样非常保守。Real-time PCR的结果表明,在干旱、低温、盐和ABA诱导下,ZmCPK9表达量均有所上调。【结论】ZmCPK9是玉米CPKs基因家族中一个新的成员,对干旱、低温、盐和ABA等非生物胁迫具有应答反应,推测ZmCPK9对植物防御非生物胁迫具有一定作用。

关键词: 玉米; CPK9基因; 非生物逆境

Abstract: 【Objective】 Calcium dependent proteinkinases (CPKs), a kind of serine/threonine protein kinases, are only found in plants and lower animals. Ca²⁺, as a second messenger, plays important roles in various aspects of plant physiology and is involved in many cellular processes. The purpose of this paper is to study the dynamic expression of ZmCPK9 gene in seedling stage under different stress conditions and to lay the foundation for further analysis of the function and mechanism of this gene in maize adverse stress response.【Method】ZmCPK9 gene sequence features were analyzed by using bioinformatics methods, and the expression characteristics of ZmCPK9 under drought, low temperature, salt and ABA stress were analyzed by real-time PCR technique. 【Result】The sequence analysis showed that ZmCPK9 had a length of 1,548 bp in the genome, encoding 515 aminoacids residues. Bioinformatics analysis revealed that the protein sequence and structure of the ZmCPK9 gene were as conservative as those of other species, the CPK gene. Real-time PCR analysis discovered that the expression of ZmCPK9 was up-regulated by ABA, cold, drought and salt stress, respectively. 【Conclusion】ZmCPK9 is a new member in corn CPKs gene family in maize. The gene is involved in signal transduction regulation under the condition of drought, low temperature, salt, and ABA stress. We speculate ZmCPK9 has a certain role in plant defense under abiotic stress.

Key words: maize; calcium-dependent protein kinase 9; abiotic stress

中图分类号:

S513
S188

足木热木·吐尔逊, 陈勋基, 陈果, 李建平, 郝晓燕, 黄全生. 玉米ZmCPK9基因在非生物胁迫下的表达分析[J]. 新疆农业科学, 2017, 54(9): 1606-1612.

Zumuremu Turxun, CHENG Xun-ji, CHEN Guo, LI Jian-ping, HAO Xiao-yan, HUANG Quan-sheng. Expression Analysis of ZmCDPK9 Gene in Maize under Abiotic Stress[J]. Xinjiang Agricultural Sciences, 2017, 54(9): 1606-1612.

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玉米ZmCPK9基因在非生物胁迫下的表达分析

Expression Analysis of ZmCDPK9 Gene in Maize under Abiotic Stress

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