玉米钙依赖蛋白激酶全基因组鉴定及抗旱表达分析

doi:10.6048/j.issn.1001-4330.2023.04.009

新疆农业科学 ›› 2023, Vol. 60 ›› Issue (4): 857-864.DOI: 10.6048/j.issn.1001-4330.2023.04.009

• 作物遗传育种·耕作栽培·种质资源 • 上一篇下一篇

玉米钙依赖蛋白激酶全基因组鉴定及抗旱表达分析

陈果(), 郝晓燕, 高升旗, 胡文冉, 赵准, 黄全生()

新疆农业科学院核技术生物技术研究所/新疆农作物生物技术重点实验室,乌鲁木齐 830091

收稿日期:2022-09-20 出版日期:2023-04-20 发布日期:2023-05-06
通信作者: 黄全生(1964-),男,乌鲁木齐人,研究员,博士,研究方向为作物抗逆分子生物学,(E-mail) hquansheng@126.com
作者简介:陈果(1984-),男,四川仪陇人,副研究员,博士,研究方向为玉米分子生物学,(E-mail)252931257@qq.com
基金资助:
自治区重大科技专项(2021A02001-2);新疆农作物生物技术重点实验室开放课题(JYS0302-2021-1)

Genome-wide identification of the maize calcium-dependent protein kinase and drought expression analysis of the CDPK gene family in maize

Chen Guo(), Hao Xiaoyan, Gao Shengqi, Hu Wenran, Zhao Zhun, Huang Quansheng()

Xinjiang Key Laboratory of Crop Biotechnology /Insititute of Nuclear and Biological Technologys, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China

Received:2022-09-20 Online:2023-04-20 Published:2023-05-06
Correspondence author: HUANG Quansheng (1964-), Male, Urumqi, researcher, doctor, research direction: molecular biology of crop resistance, (E-mail) hquansheng@126.com
Supported by:
Major Science and Technology Projects of the Autonomous Region(2021A02001-2);Open Pooject of Xinjiang Crop Biotechnology Key Laboratory(JYS0302-2021-1)

摘要/Abstract

摘要：

【目的】分析研究玉米钙依赖蛋白激酶全基因组鉴定及抗旱表达。【方法】使用NCBI Blast、DNAMAN和MotifScan等程序分析玉米钙依赖蛋白激酶CDPK的蛋白结构域和系统发育。采用Real-time RT-PCR方法分析CDPK基因在干旱胁迫处理玉米幼苗中的表达,评价CDPK基因对玉米干旱胁迫反应能力。【结果】鉴定出了在玉米中含有39个CDPK基因。将CDPK基因分为4个组。每个群体中的成员有共同的蛋白质基序和外显子及内含子结构,共同的进化起源。39个玉米CDPK基因根据它们的系统发育关系分组,并锚定在特定的玉米染色体上。多数CDPK基因在玉米干旱胁迫中的表达水平发生改变,CDPK基因参与对干旱胁迫的响应。【结论】从玉米基因组数据库中鉴定出了39个CDPK基因,大多数玉米CDPK基因在不同组织和不同发育阶段表现出不同的表达水平,CDPK基因在玉米发育中发挥不同的作用。多数CDPK基因在干旱胁迫下的表达量均发生变化。

关键词: 钙依赖蛋白激酶; 全基因组鉴定; 干旱胁迫; 表达分析; 玉米

Abstract:

【Objective】Genome-wide identification of the maize calcium-dependent protein kinase and drought expression analysis of the CDPK gene family in maize.【Methods】NCBI Blast, DNAMAN and MotifScan were used to analyze the protein domains and phylogeny of CDPK in maize by genome-wide analysis. Real-time RT-PCR was performed to analyze the expression of CDPK gene in maize seedlings under drought stress, and to evaluate the effect of CDPK gene on maize response to drought stress.【Results】Through bioinformatics analysis of the whole genome of maize, 39 CDPK genes were identified in maize. According to phylogenetic analysis of CDPKs in maize, rice and Arabidopsis thaliana, these CDPK genes were divided into four groups. The members of each group share a common protein motif and exon and intron structure, suggesting that they may have a common evolutionary origin. The 39 maize CDPK genes are grouped according to their phylogenetic relationships and are anchored to specific maize chromosomes. It was found that the expression levels of most CDPK genes changed in maize under drought stress, indicating the response of these CDPK genes to drought stress.【Conclusion】39 CDPK genes were identified from maize genome database. Most of the CDPK genes in maize showed different expression levels in different tissues and different development stages, indicating that CDPK genes play different roles in maize development. At the same time, the expression levels of most CDPK genes changed under drought stress, which laid a foundation for further screening and studying the function of CDPK gene family under drought stress.

Key words: calcium-dependent protein kinase; genome-wide identification; drought stress; expression analysis; maize

中图分类号:

S188

陈果, 郝晓燕, 高升旗, 胡文冉, 赵准, 黄全生. 玉米钙依赖蛋白激酶全基因组鉴定及抗旱表达分析[J]. 新疆农业科学, 2023, 60(4): 857-864.

Chen Guo, Hao Xiaoyan, Gao Shengqi, Hu Wenran, Zhao Zhun, Huang Quansheng. Genome-wide identification of the maize calcium-dependent protein kinase and drought expression analysis of the CDPK gene family in maize[J]. Xinjiang Agricultural Sciences, 2023, 60(4): 857-864.

图/表 4

参考文献 32

[1]	梁晓玲, 刘文欣, 阿布来提·阿布拉, 等. 干旱胁迫对玉米杂交种产量及穗部性状的影响[J]. 玉米科学, 2021, 29(2):75-80.
	Liang Xiaoling, Liu Wenxin, Abulaiti Abra, et al. Influence of drought stress on yield and ear traits of maize hybrids[J]. Journal of Maize Sciences, 2021, 29(2):75-80.
[2]	Hu H, Xiong L. Genetic engineering and breeding of drought-resistant crops[J]. Annu Rev Plant Biol, 2014,(65):715-741.
[3]	Tuteja N, Mahajan S. Calcium signaling network in plants: an overview[J]. Plant Signal Behav, 2007, (2):79-85. DOI PMID
[4]	Harper JF, Harmon A. Plants symbiosis and parasites: a calcium signaling connection[J]. Nature Rev, 2005, (6):555-566.
[5]	Lourido S, Shuman J, Zhang C, et al. Calcium dependent protein kinase 1 is an essential regulator of exocytosis in Toxoplasma[J]. Nature, 2010, (465):359-362.
[6]	Klimecka M, Muszynska G. Structure and functions of plant calcium dependent protein kinases[J]. Acta Biochim Pol, 2007, 54:219-233. PMID
[7]	Romeis T, Ludwig AA, Martin R, et al. Calcium-dependent protein kinases play an essential role in a plant defence response[J]. EMBO J, 2001, (20):5556-5567. DOI PMID
[8]	Asano T, Hayashi N, Kikuchi S, et al. CDPK-mediated abiotic stress signaling[J]. Plant Signal Behav, 2012, (7):1-5.
[9]	Lee J, Rudd JJ. Calcium-dependent protein kinases: versatile plant signaling components necessary for pathogen defence[J]. Trends Plant Sci, 2002, (7):97-98.
[10]	Ludwig AA, Romeis T, Jones JD. CDPK-mediated signalling pathways: specificity and cross-talk[J]. J Exp Bot, 2004, (55):181-188.
[11]	Zhu SY, Yu XC, Wang XJ, et al. Two calcium-dependent protein kinases CPK4 and CPK11 regulate abscisic acid signal transduction in Arabidopsis[J]. Plant Cell, 2007, (19):3019-3036.
[12]	Mori IC, Murata Y, Yang Y, et al. CDPKs CPK6 and CPK3 function in ABA regulation of guard cell S-type anion- and Ca²⁺-permeable channels and stomatal closure[J]. PLoS Biol, 2006, (4): e327.
[13]	Munemasa S, Hossain MA, Nakamura Y, et al. The Arabidopsis calcium-dependent protein kinase CPK6 functions as a positive regulator of methyl jasmonate signaling in guard cells[J]. Plant Physiol, 2011, (155):553-561.
[14]	Ma SY, Wu WH. AtCPK23 functions in Arabidopsis responses to drought and salt stresses[J]. Plant Mol Biol, 2007, (65):511-518.
[15]	Franz S, Ehlert B, Liese A, et al. Calcium-dependent protein kinase CPK21 functions in abiotic stress response in Arabidopsis thaliana[J]. Mol Plant, 2011, (4):83-96.
[16]	Choi HI, Park HJ, Park JH, et al. Arabidopsis calcium-dependent protein kinase AtCPK32 interacts with ABF4 a transcriptional regulator of abscisic acid-responsive gene expression and modulates its activity[J]. Plant Physiol, 2005, 139:1750-1761. DOI URL
[17]	Boudsocq M, Willmann MR, McCormack M, et al. Differential innate immune signaling via Ca²⁺sensor protein kinases[J]. Nature, 2010, (464):418-422.
[18]	Saijo Y, Hata S, Kyozuka J, et al. Over-expression of a single Ca²⁺dependent protein kinase confers both cold and salt/drought tolerance on rice plants[J]. Plant J, 2000, (23):319-327.
[19]	Asano T, Hakata M, Nakamura H, et al. Functional characterisation of OsCPK21 a calcium-dependent protein kinase that confers salt tolerance in rice[J]. Plant Mol Biol, 2011, (75):179-191.
[20]	Asano T, Hayashi N, Kobayashi M, et al. A rice calcium-dependent protein kinase OsCPK12 oppositely modulates salt-stress tolerance and blast disease resistance[J]. Plant J, 2012, (69):26-36.
[21]	Cheng SH, Willmann MR, Chen HC, et al. Calcium signaling through protein kinases: The Arabidopsis calcium-dependent protein kinase gene family[J]. Plant Physiol, 2002, (129):469-485.
[22]	Asano T, Tanaka N, Yang G, et al. Genome-wide identification of the rice calcium-dependent protein kinase and its closely related kinase gene families: comprehensive analysis of the CDPKs gene family in rice[J]. Plant Cell Physiol, 2005, (46):356-366
[23]	Li AL, Zhu YF, Tan XM, et al. Evolutionary and functional study of the CDPK gene family in wheat (Triticum aestivum L)[J]. Plant Mol Biol, 2008, (66):429-443.
[24]	Yang DH, Hettenhausen C, Baldwin IT, et al. Nicotiana attenuata calcium-dependent protein kinases CDPK4 and CDPK5 strongly downregulate wound- and herbivory-induced jasmonic acid accumulations[J]. Plant Physiol, 2012, (159):1561-1607.
[25]	Giammaria V, Grandellis C, Bachmann S, et al. StCDPK2 expression and activity reveal a highly responsive potato calcium-dependent protein kinase involved in light signaling[J]. Planta, 2011, (233):593-609.
[26]	Chang WJ, Su HS, Li WJ, et al. Expression profiling of a novel calcium-dependent protein kinase gene LeCPK2 from tomato (Solanum lycopersicum) under heat and pathogen-related hormones[J]. Biosci Biotech Bioch, 2009, (73):2427-2431.
[27]	Berberich T, Kusano T. Cycloheximide induces a subset of low temperature-inducible genes in maize[J]. Mol Gen Genet, 1997, (254):275-283.
[28]	Saijo Y, Hata S, Sheen J, et al. cDNA cloning and prokaryotic expression of maize calcium-dependent protein kinases[J]. Biochim Biophys Acta, 1997, (1350):109-114.
[29]	Murillo I, Jaeck E, Cordero MJ, et al. Transcriptional activation of a maize calcium-dependent protein kinase gene in response to fungal elicitors and infection[J]. Plant Mol Biol, 2001, (45):145-158.
[30]	Klimecka M, Szczegielniak J, Godecka L, et al. Regulation of wound-responsive calcium dependent protein kinase from maize (ZmCPK11) by phosphatidic acid[J]. Acta Biochim Pol, 2011, (58):589-595.
[31]	Schnable PS, Ware D, Fulton RS, et al. The B73 maize genome: complexity diversity and dynamics[J]. Science, 2009, (326):1112-1115.
[32]	Matschi S, Werner S, Schulze WX, et al. Function of calcium-dependent protein kinase CPK28 of Arabidopsis thaliana in plant stem elongation and vascular development[J]. Plant J, 2013, (73):883-896.

名称 Name	序列号 ID	CDs	氨基酸 Amino acid	分子量 MW (kDa)	EF手型数量 No. of EF Hands	N端序列 N-terminal	N端豆蔻酰化基元 N-Myristoylation
ZmCDPK1	GRMZM2G028926_T01	1 827	608	66.4	4	MGNTCVGP	No
ZmCDPK2	GRMZM2G320506_T01	1 863	620	67.7	4	MGNTCVGP	No
ZmCDPK3	GRMZM2G025387_T01	1 593	530	59.9	4	MGNRASRH	Yes
ZmCDPK4	GRMZM2G035843_T01	1 527	508	56.5	4	MQPDPSGN	No
ZmCDPK5	GRMZM2G314396_T01	1 644	547	60.4	4	MGNACSGA	Yes
ZmCDPK6	GRMZM2G321239_T01	1 671	556	61.2	4	MGNACGGA	Yes
ZmCDPK7	GRMZM2G104125_T01	1 608	535	60.3	4	MGNCCATP	Yes
ZmCDPK8	AC210013.4_FGP014	1 617	538	60.4	4	MGNCCAAP	Yes
ZmCDPK9	GRMZM2G154489_T01	1 596	531	59.4	4	MGQCCSRA	Yes
ZmCDPK10	GRMZM2G030673_T01	1 626	541	60.5	4	MGNCCRSP	No
ZmCDPK11	GRMZM2G463464_T01	1 548	515	56.8	4	MQPDPQGP	No
ZmCDPK12	GRMZM2G047486_T01	1 533	510	60.2	4	MQPDPSGN	No
ZmCDPK13	GRMZM2G311220_T01	1 611	536	60.2	4	MGNCCRSP	No
ZmCDPK14	GRMZM2G028086_T01	1 620	539	60.8	4	MGNCCVTP	Yes
ZmCDPK15	GRMZM2G058305_T01	1 620	539	60.7	4	MGGRASRH	Yes
ZmCDPK16	GRMZM2G015703_T01	1 779	592	65.4	1	MGLCHGKP	Yes
ZmCDPK17	GRMZM2G167276_T01	1 533	510	56.2	4	MGNCCPGS	Yes
ZmCDPK18	GRMZM2G157068_T02	1 569	522	58.5	4	MGACFSSA	Yes
ZmCDPK19	GRMZM2G347047_T01	1 467	488	53.9	4	MGGHQLHL	No
ZmCDPK20	GRMZM2G121228_T01	1 743	580	63.7	4	MGNTCVGP	No
ZmCDPK21	GRMZM2G027351_T01	1 755	584	64.1	4	MGNTCVGP	No
ZmCDPK22	GRMZM2G347226_T01	1 548	515	56.8	4	MQPDPQGS	No
ZmCDPK23	GRMZM2G040743_T01	1 623	540	60.9	4	MGNQCQNG	No
ZmCDPK24	GRMZM2G080871_T02	1 536	511	57.0	3	MGGCHSAI	No
ZmCDPK25	GRMZM2G353957_T01	1 941	646	71.7	4	MGNVCVGP	No
ZmCDPK26	GRMZM2G081310_T01	1 689	562	61.9	4	MGNTCGVT	No
ZmCDPK27	GRMZM2G032852_T02	1 635	544	61.7	4	MGNQCPNG	No
ZmCDPK28	GRMZM2G365035_T01	1 539	512	57.5	4	MGLCSSST	Yes
ZmCDPK29	GRMZM2G112057_T01	1 620	539	61.0	4	MGNCFTRK	Yes
ZmCDPK30	GRMZM2G088361_T01	1 623	540	60.2	4	MGNCCRSP	No
ZmCDPK31	GRMZM5G856738_T03	1 575	524	59.5	4	MGNRASRH	Yes
ZmCDPK32	GRMZM2G099425_T01	1 620	539	60.8	4	MGNCCVTP	Yes
ZmCDPK33	GRMZM2G168706_T01	1 596	531	59.4	4	MGQCCSRA	Yes
ZmCDPK34	GRMZM2G340224_T01	1 842	613	67.5	4	MRPSVSMI	Yes
ZmCDPK35	GRMZM2G365815_T01	1 659	552	60.0	4	MGQCCSKG	Yes
ZmCDPK36	GRMZM2G472311_T01	1 746	581	63.4	4	MGQCCSKG	Yes
ZmCDPK37	AC203294.3_FGP001	1 395	464	50.9	4	MGNCCPGS	No
ZmCDPK38	GRMZM2G332660_T01	1 707	568	62.8	4	MGGCYSAF	Yes
ZmCDPK39	GRMZM2G097533_T01	1 317	438	48.5	1	MGNCCVSR	Yes

名称 Name	序列号 ID	CDs	氨基酸 Amino acid	分子量 MW (kDa)	EF手型数量 No. of EF Hands	N端序列 N-terminal	N端豆蔻酰化基元 N-Myristoylation
ZmCDPK1	GRMZM2G028926_T01	1 827	608	66.4	4	MGNTCVGP	No
ZmCDPK2	GRMZM2G320506_T01	1 863	620	67.7	4	MGNTCVGP	No
ZmCDPK3	GRMZM2G025387_T01	1 593	530	59.9	4	MGNRASRH	Yes
ZmCDPK4	GRMZM2G035843_T01	1 527	508	56.5	4	MQPDPSGN	No
ZmCDPK5	GRMZM2G314396_T01	1 644	547	60.4	4	MGNACSGA	Yes
ZmCDPK6	GRMZM2G321239_T01	1 671	556	61.2	4	MGNACGGA	Yes
ZmCDPK7	GRMZM2G104125_T01	1 608	535	60.3	4	MGNCCATP	Yes
ZmCDPK8	AC210013.4_FGP014	1 617	538	60.4	4	MGNCCAAP	Yes
ZmCDPK9	GRMZM2G154489_T01	1 596	531	59.4	4	MGQCCSRA	Yes
ZmCDPK10	GRMZM2G030673_T01	1 626	541	60.5	4	MGNCCRSP	No
ZmCDPK11	GRMZM2G463464_T01	1 548	515	56.8	4	MQPDPQGP	No
ZmCDPK12	GRMZM2G047486_T01	1 533	510	60.2	4	MQPDPSGN	No
ZmCDPK13	GRMZM2G311220_T01	1 611	536	60.2	4	MGNCCRSP	No
ZmCDPK14	GRMZM2G028086_T01	1 620	539	60.8	4	MGNCCVTP	Yes
ZmCDPK15	GRMZM2G058305_T01	1 620	539	60.7	4	MGGRASRH	Yes
ZmCDPK16	GRMZM2G015703_T01	1 779	592	65.4	1	MGLCHGKP	Yes
ZmCDPK17	GRMZM2G167276_T01	1 533	510	56.2	4	MGNCCPGS	Yes
ZmCDPK18	GRMZM2G157068_T02	1 569	522	58.5	4	MGACFSSA	Yes
ZmCDPK19	GRMZM2G347047_T01	1 467	488	53.9	4	MGGHQLHL	No
ZmCDPK20	GRMZM2G121228_T01	1 743	580	63.7	4	MGNTCVGP	No
ZmCDPK21	GRMZM2G027351_T01	1 755	584	64.1	4	MGNTCVGP	No
ZmCDPK22	GRMZM2G347226_T01	1 548	515	56.8	4	MQPDPQGS	No
ZmCDPK23	GRMZM2G040743_T01	1 623	540	60.9	4	MGNQCQNG	No
ZmCDPK24	GRMZM2G080871_T02	1 536	511	57.0	3	MGGCHSAI	No
ZmCDPK25	GRMZM2G353957_T01	1 941	646	71.7	4	MGNVCVGP	No
ZmCDPK26	GRMZM2G081310_T01	1 689	562	61.9	4	MGNTCGVT	No
ZmCDPK27	GRMZM2G032852_T02	1 635	544	61.7	4	MGNQCPNG	No
ZmCDPK28	GRMZM2G365035_T01	1 539	512	57.5	4	MGLCSSST	Yes
ZmCDPK29	GRMZM2G112057_T01	1 620	539	61.0	4	MGNCFTRK	Yes
ZmCDPK30	GRMZM2G088361_T01	1 623	540	60.2	4	MGNCCRSP	No
ZmCDPK31	GRMZM5G856738_T03	1 575	524	59.5	4	MGNRASRH	Yes
ZmCDPK32	GRMZM2G099425_T01	1 620	539	60.8	4	MGNCCVTP	Yes
ZmCDPK33	GRMZM2G168706_T01	1 596	531	59.4	4	MGQCCSRA	Yes
ZmCDPK34	GRMZM2G340224_T01	1 842	613	67.5	4	MRPSVSMI	Yes
ZmCDPK35	GRMZM2G365815_T01	1 659	552	60.0	4	MGQCCSKG	Yes
ZmCDPK36	GRMZM2G472311_T01	1 746	581	63.4	4	MGQCCSKG	Yes
ZmCDPK37	AC203294.3_FGP001	1 395	464	50.9	4	MGNCCPGS	No
ZmCDPK38	GRMZM2G332660_T01	1 707	568	62.8	4	MGGCYSAF	Yes
ZmCDPK39	GRMZM2G097533_T01	1 317	438	48.5	1	MGNCCVSR	Yes

玉米钙依赖蛋白激酶全基因组鉴定及抗旱表达分析

Genome-wide identification of the maize calcium-dependent protein kinase and drought expression analysis of the CDPK gene family in maize

在线阅读

PDF下载

可视化

摘要/Abstract

引用本文

使用本文

图/表 4

参考文献 32

相关文章 15

编辑推荐

Metrics

[1]	王晓雨, 王小平, 史文宇, 刘美艳, 马健, 郭云鹏, 宋瑞欣, 王清涛. 拔节期冬小麦光合特性、干物质积累和产量对干旱胁迫的响应[J]. 新疆农业科学, 2023, 60(9): 2163-2172.
[2]	向莉, 王仙, 董裕生, 郭小玲, 方伏荣, 陈智军, 马艳明, 苗雨. 外源丁酸对干旱胁迫下大麦产量及品质的影响[J]. 新疆农业科学, 2023, 60(9): 2173-2181.
[3]	米尔扎提·木塔力甫, 石秀楠, 柏军兵, 祖拜代·阿布都克日木, 吾勒加勒哈斯·阿扎提, 石书兵. 不同脱绒方式及PEG胁迫下对棉花种子活力及幼苗性状的影响[J]. 新疆农业科学, 2023, 60(7): 1561-1568.
[4]	王挺, 张力, 张凡凡, 黄嵘峥, 李肖, 张玉琳, 陈永成, 赵建涛, 马春晖. 适合青贮的玉米品种生产性能筛选及营养价值评价[J]. 新疆农业科学, 2023, 60(7): 1596-1605.
[5]	陈占辉, 孙强, 任姣姣, 黄博文, 许加波, 杨杰, 吴鹏昊. 玉米叶宽的QTL定位及全基因组选择分析[J]. 新疆农业科学, 2023, 60(7): 1606-1613.
[6]	蓝陈仪航, 姚禹博, 周俊祥, 付开赟, 丁新华, 尹晓辉, 刘文, 王娜, 郭文超, 邓建宇. 亚洲玉米螟性信息素鉴定及其应用研究进展[J]. 新疆农业科学, 2023, 60(7): 1614-1622.
[7]	曲可佳, 时晓磊, 张恒, 王兴州, 耿洪伟, 丁孙磊, 张金波, 严勇亮. PEG处理下引进春小麦品种苗期抗旱性评价[J]. 新疆农业科学, 2023, 60(6): 1363-1371.
[8]	邵盘霞, 赵准, 邵武奎, 郝晓燕, 高升旗, 李建平, 胡文冉, 黄全生. 玉米ZmCDPK22基因在干旱胁迫下的表达分析[J]. 新疆农业科学, 2023, 60(6): 1372-1378.
[9]	陆晏天, 桑志勤, 徐灿, 张力, 夏春兰, 王友德, 李伟, 陈树宾. 历年新疆和宁夏审定玉米品种主要性状的演化及品种审定现状分析[J]. 新疆农业科学, 2023, 60(6): 1379-1388.
[10]	耿翡翡, 孟超敏, 卿桂霞, 周佳敏, 张富厚, 刘逢举. 陆地棉磷高效基因GhMYB4的克隆与表达分析[J]. 新疆农业科学, 2023, 60(6): 1406-1412.
[11]	汤东, 安玉光, 程平, 李宏, 杨建军, 王凯. 天山北坡前山带典型灌木光合特性对干旱胁迫的响应[J]. 新疆农业科学, 2023, 60(6): 1531-1539.
[12]	杨明花, 刘强, 廖必勇, 彭云承, 布阿依夏木·那曼提, 达吾来·杰克山. 不完全双列杂交玉米组合抗倒伏综合评价[J]. 新疆农业科学, 2023, 60(4): 832-840.
[13]	周广威, 韩登旭, 朱琦, 张少民. 耐低磷新疆春玉米基因型筛选及其磷效率[J]. 新疆农业科学, 2023, 60(4): 847-856.
[14]	董秀丽, 韩登旭, 杨杰, 阿布来提·阿布拉, 戴爱梅, 李俊杰, 王业建, 刘俊, 郗浩江, 梁晓玲, 李铭东. 密植条件下玉米主要农艺性状的综合性分析[J]. 新疆农业科学, 2023, 60(4): 865-871.
[15]	侯良忠, 郭同军, 张俊瑜, 苏玲玲, 古再丽努尔·艾麦提, 温小燕, 朱晓芳, 杨建中, 王文奇. 不同比例籽用西葫芦皮瓤与玉米秸秆混贮效果评价[J]. 新疆农业科学, 2023, 60(3): 567-573.