玉米ZmCDPK22基因在干旱胁迫下的表达分析

doi:10.6048/j.issn.1001-4330.2023.06.009

摘要/Abstract

摘要：

【目的】研究玉米ZmCDPK22基因的功能特征,分析该基因的编码序列,分析物种间的进化关系及蛋白互作及其功能,以叶片含水量作为干旱胁迫指标,研究ZmCDPK22在干旱胁迫下的表达特性。【方法】利用生物信息学手段分析该基因的基本特征,运用实时荧光定量(Quantitative Real-time PCR,qRT-PCR)技术分析该基因在干旱胁迫下的表达特性。【结果】该基因编码一个分子式为C₂₆₇₅H₄₂₁₇N₇₇₉O₇₉₈S₂₁,分子量为60 731.91,氨基酸大小为539 aa,等电点为6.44的亲水性蛋白。该蛋白无跨膜结构以及信号肽,预测结果显示该蛋白定位于细胞核且与10个蛋白存在互作,属于PK_like亚家族;该基因启动区含有大量的光响应和多种激素响应等元件。在不同含水量情况下,ZmCDPK22的表达量不同,与叶片含水量87%相比,其相对表达量随着叶片含水量的降低而逐渐降低,在叶片含水量为78%时相对表达量最高,在含水量为33%时相对表达量最低。【结论】该基因在叶片不同含水量的表达量不同,该基因响应干旱胁迫,可能在干旱应答反应中发挥一定功能。

关键词: 玉米; ZmCDPK22; 干旱胁迫; 生物信息学; 基因表达

Abstract:

【Objective】 In order to explore the functional characteristics of ZmCDPK22 gene in maize. We predict the function of ZmCDPK22 theoretically through analysing evolutionary relationships among species and protein interactions. Taking water content of leaf as an index of drought stress to determine the expression characteristics of ZmCDPK22 under drought stress. 【Methods】 The basic characteristics of ZmCDPK22 and its relative expression patterns in response to drought stress were analyzed by bioinformatics and RT-qPCR. 【Results】 The ZmCDPK22 protein had a molecular weight of 60,731.91 and a theoretical isoelectric point of 6.44 and which encoding a hydrophilic protein with molecular formula C₂₆₇₅H₄₂₁₇N₇₇₉O₇₉₈S₂₁and 539 amino acids. The protein was mainly localized in the nucleus and had no signal peptide and transmembrane structure. Protein-protein interaction prediction result showed that ZmCDPK22 could interact with ten proteins, and it belongs to the PK_like subfamily. The promoter region of ZmCDPK22 containd a large number of Cis-acting elements such as light response and hormone response. qRT-PCR results revealed that the expression of ZmCDPK22 was different under different water content. Compared with 87%water content of leaf, the relative expression of ZmcDPK22 decreased gradually with the decrease of water content of leavf. The relative expression of ZmcDPK22 was the highest when the water content was 78%, and the lowest when the water content was 33%. 【Conclusion】 The expression of this gene was different in different water content of leaves, indicating that this gene was responsive to drought stress and might play a role in drought response.

Key words: maize; ZmCDPK22; drought stress; bioinformation; gene expression

中图分类号:

S513

邵盘霞, 赵准, 邵武奎, 郝晓燕, 高升旗, 李建平, 胡文冉, 黄全生. 玉米ZmCDPK22基因在干旱胁迫下的表达分析[J]. 新疆农业科学, 2023, 60(6): 1372-1378.

SHAO Panxia, ZHAO Zhun, SHAO Wukui, HAO Xiaoyan, GAO Shengqi, LI Jianping, HU Wenran, HUANG Quansheng. Expression analysis of ZmCDPK22 gene in maize under drought stress[J]. Xinjiang Agricultural Sciences, 2023, 60(6): 1372-1378.

图/表 7

参考文献 28

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元件 Element	数量 Number	功能 Function	元件 Element	数量 Number	功能 Function
TATA-box	30	核心启动元件	GT1-motif	1	光响应元件
CAAT-box	26	核心启动元件	G-box	1	光响应元件
STRE	6	unknown	DRE core	1	unknown
MYC	5	unknown	AT-rich element	1	富含AT的DNA蛋白结合位点
as-1	3	unknown	A-box	1	顺式作用调控元件
CGTCA-motif	3	茉莉酸甲酯响应元件	W box	1	unknown
TGACG-motif	3	茉莉酸甲酯响应元件	CCGTCC motif	1	unknown
ARE	3	厌氧诱导元件	TCT-motif	1	光响应元件
ABRE	2	脱落酸响应元件	Box 4	1	参与光反应
ACE	2	光响应元件	G-Box	1	光响应元件
AAGAA-motif	2	unknown	WUN-motif	1	unknown
Sp1	2	光响应元件	H-box	1	unknown
AT~TATA-box	2	unknown	ERE	1	unknown
LTR	1	低温响应元件	CAT-box	1	分生组织表达元件
CCGTCC-box	1	unknown	WRE3	1	unknown
Myc	1	unknown

元件 Element	数量 Number	功能 Function	元件 Element	数量 Number	功能 Function
TATA-box	30	核心启动元件	GT1-motif	1	光响应元件
CAAT-box	26	核心启动元件	G-box	1	光响应元件
STRE	6	unknown	DRE core	1	unknown
MYC	5	unknown	AT-rich element	1	富含AT的DNA蛋白结合位点
as-1	3	unknown	A-box	1	顺式作用调控元件
CGTCA-motif	3	茉莉酸甲酯响应元件	W box	1	unknown
TGACG-motif	3	茉莉酸甲酯响应元件	CCGTCC motif	1	unknown
ARE	3	厌氧诱导元件	TCT-motif	1	光响应元件
ABRE	2	脱落酸响应元件	Box 4	1	参与光反应
ACE	2	光响应元件	G-Box	1	光响应元件
AAGAA-motif	2	unknown	WUN-motif	1	unknown
Sp1	2	光响应元件	H-box	1	unknown
AT~TATA-box	2	unknown	ERE	1	unknown
LTR	1	低温响应元件	CAT-box	1	分生组织表达元件
CCGTCC-box	1	unknown	WRE3	1	unknown
Myc	1	unknown