生长素与高粱杂种优势关系分析

doi:10.6048/j.issn.1001-4330.2023.04.007

新疆农业科学 ›› 2023, Vol. 60 ›› Issue (4): 841-846.DOI: 10.6048/j.issn.1001-4330.2023.04.007

• 作物遗传育种·耕作栽培·种质资源 • 上一篇下一篇

生长素与高粱杂种优势关系分析

商靖¹(), 逄洪波¹, 王兰兰¹, 李雪梅¹, 王艳秋²(), 李玥莹¹()

1.沈阳师范大学生命科学学院,沈阳 110034
2.辽宁省农业科学院,沈阳 110161

收稿日期:2022-07-30 出版日期:2023-04-20 发布日期:2023-05-06
通信作者: 王艳秋(1973-),女,辽宁葫芦岛人,研究员,硕士,研究方向为高粱育种与栽培,(E-mail)wangyanqiu73@126.com;李玥莹(1966-),女,辽宁沈阳人,教授,博士,硕士生/博士生导师,研究方向为基因工程,(E-mail)yueyinglicn@163.com
作者简介:商靖(1996-),女,辽宁北票人,硕士研究生,研究方向为植物基因工程,(E-mail)1789349384@qq.com
基金资助:
辽宁省教育厅重点攻关项目(LZD201901);辽宁省教育厅自然科学重点攻关项目(LZD202004);辽宁省教育厅科学研究项目(LJKZ0991)

Study on the relationship between auxin and sorghum heterosis

SHANG Jing¹(), PANG Hongbo¹, WANG Lanlan¹, LI Xuemei¹, WANG Yanqiu²(), LI Yueying¹()

1. School of Life Sciences, Shenyang Normal University, Shenyang 110034, China
2. Liaoning Academy of Agricultural Sciences, Shenyang 110161, China

Received:2022-07-30 Online:2023-04-20 Published:2023-05-06
Correspondence author: WANG Yanqiu(1973-), female, from Huludao, Liaoning, researcher, master, engaged in sorghum breeding and cultivation, (E-mail) wangyanqiu73@126.com;LI Yueying(1966-), female, from Shenyang, Liaoning Province, professor, Ph.D., postgraduate tutor, research direction is genetic engineering, (E-mail) yueyinglicn@163.com
Supported by:
key R & D Project of the Education Department of Liaoning Province(LZD201901);Key Research Project of Natural Science of the Education Department of Liaoning Province(LZD202004);Scientific Research Project of the Education Department of Liaoning Province(LJKZ0991)

摘要/Abstract

摘要：

【目的】研究高粱生长素(IAA)与杂种优势之间的潜在关系,为高粱育种中杂种优势的利用提供重要理论依据。【方法】以11个亲本及组配的11个杂交种为材料,采用高效液相色谱技术测定杂交种及其亲本生长素在抽穗期和成熟期的含量,利用实时荧光定量PCR技术比较抽穗期、成熟期高粱不同品种的生长素基因表达的差异。【结果】11个杂交种成熟期的生长素含量均高于抽穗期,成熟期杂种辽粘3号、辽杂19、辽杂35、辽杂36、辽2297、辽2697、辽5397、辽糯7号、辽糯11号、辽夏梁1号的生长素含量与其亲本相比,子代生长素含量更高,成熟期只有辽粘6号的生长素含量比其亲本的低。以对应的母本或父本为对照,杂交种辽粘3号和辽杂19在抽穗期的生长素基因表达量低于其亲本,杂交种辽粘3号、辽粘6号、辽杂36在成熟期的生长素基因表达量低于其亲本,其余杂交品种在抽穗期、成熟期生长素基因表达量均高于其亲本。【结论】内源生长素是调节高粱生长杂种优势的一个因素,但不是唯一因素。

关键词: 高粱; 杂种优势; 生长素; 基因表达; 实时荧光定量PCR技术

Abstract:

【Objective】In To explore the potential relationship between sorghum auxin (IAA) and heterosis, and provide an important theoretical basis for the utilization of heterosis in sorghum breeding in the future.【Methods】With 11 parents and 11 hybrids as research materials, the content of auxin in the hybrids and their parents at the heading and maturity stages was determined by high performance liquid chromatography, and the heading dates were compared by real-time fluorescent quantitative PCR technology, which was used again to compare the differences in auxin gene expression of different varieties of sorghum at the heading and maturity stages.【Results】The results showed that the auxin content of the 11 hybrids at the mature stage was higher than that at the heading stage. The auxin content of Liaoning 3, Liaoza 19, Liaoza 35, Liaoza 36, Liao 2297, Liao 2697, Liao 5397, Liaonuo 7, Liaonuo 11, Liaoxialiang 1 and their parents in mature period. In contrast, the progeny had higher auxin content, and only Liaoning 6 had lower auxin content than its parents at maturity. When the corresponding female parent or male parent was taken as the control, the auxin gene expression levels of Liaoning 3 and Liaoza 19 were lower than their parents during the heading stage. At the maturity stage, the auxin gene expression levels of Liaoning 3, Liaoning 6 and Liaoza 36 were lower than their parents. The expression levels of auxin genes in other hybrids were higher than their parents.【Conclusion】The finding shows that endogenous auxin is a factor that regulates the heterosis of sorghum growth, but not the only one.

Key words: sorghum; hybrid advantage; auxin; gene expression; QRT-PCR

中图分类号:

S514

商靖, 逄洪波, 王兰兰, 李雪梅, 王艳秋, 李玥莹. 生长素与高粱杂种优势关系分析[J]. 新疆农业科学, 2023, 60(4): 841-846.

SHANG Jing, PANG Hongbo, WANG Lanlan, LI Xuemei, WANG Yanqiu, LI Yueying. Study on the relationship between auxin and sorghum heterosis[J]. Xinjiang Agricultural Sciences, 2023, 60(4): 841-846.

图/表 12

图1 母本在抽穗期、成熟期生长素含量变化

Fig.1 Changes of auxin content in female parent during heading and maturity

图2 杂交种在抽穗期、成熟期生长素含量变化

Fig.2 Changes of auxin content in hybrids at heading and maturity stages

图3 母本在抽穗期、成熟期生长素含量变化

Fig.3 Changes of auxin content in female parent during heading and maturity

图4 杂交种在抽穗期、成熟期生长素含量变化

Fig.4 Changes of auxin content in hybrids at heading and maturity stages

图5 父本在抽穗期、成熟期生长素含量变化

Fig.5 Changes of auxin content in male parents at heading and maturity stages

图6 杂交种在抽穗期、成熟期生长素含量变化

Fig.6 Changes of auxin content in hybrids at heading and maturity stages

图7 父本在抽穗期、成熟期生长素含量变化

Fig.7 Changes of auxin content in male parents at heading and maturity stages

图8 杂交种在抽穗期、成熟期生长素含量变化

Fig.8 Changes of auxin content in hybrids at heading and maturity stages

图9 母本与杂交种在抽穗期、成熟期IAA基因的表达量

Fig.9 IAA gene expression levels of female parent and hybrid at heading and maturity stages

图10 母本与杂交种在抽穗期、成熟期IAA基因的表达量

Fig.10 IAA gene expression levels of female parent and hybrid at heading and maturity stages

图11 父本与杂交种在抽穗期、成熟期IAA基因的表达量

Fig.11 IAA gene expression levels of male parent and hybrid at heading and maturity stages

图12 父本与杂交种在抽穗期、成熟期IAA基因的表达量

Fig.12 IAA gene expression levels of male parent and hybrid at heading and maturity stages

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生长素与高粱杂种优势关系分析

Study on the relationship between auxin and sorghum heterosis

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