亚洲玉米螟的抗药性监测及双酰胺类药剂抗性靶标分子检测

doi:10.6048/j.issn.1001-4330.2021.04.012

新疆农业科学 ›› 2021, Vol. 58 ›› Issue (4): 690-699.DOI: 10.6048/j.issn.1001-4330.2021.04.012

• 土壤肥料·植物保护·微生物 • 上一篇下一篇

亚洲玉米螟的抗药性监测及双酰胺类药剂抗性靶标分子检测

支昊宇¹, 王智慧², 王兴仔¹, 丁新华³, 贾尊尊³, 郭文超², 姜卫华¹

1.南京农业大学植物保护学院,南京 210095;
2.新疆农业科学院微生物应用研究所/新疆特殊环境微生物实验室,乌鲁木齐 830091;
3.新疆农业科学院植物保护研究所/农业部西北荒漠绿洲作物有害生物综合治理重点实验室,乌鲁木齐 830091

收稿日期:2020-12-01 出版日期:2021-04-20 发布日期:2021-04-25
通信作者: 姜卫华(1969-),女,江苏人,副教授,硕士生导师,研究方向为昆虫分子生物学及生理毒理学,(E-mail)jwh@ njau.edu.cn
郭文超(1966-),男,河北人,研究员,博士生导师,研究方向为农业害虫综合防治,(E-mail)gwc1966@163.com
作者简介:支昊宇(1994-),男,硕士研究生,研究方向为昆虫生理生化与分子生物学,(E-mail)1415031496@qq.com
基金资助:
国家重点研发计划(2017YFD0201800)

Insecticide Resistance Monitoring and Target Molecular Detection of Diamide Insecticides in Field Populations of Ostriniafurnacalis

ZHI Haoyu¹, WANG Zhihui², WANG Xingzai¹, DING Xinhua³, JIA Zunzun³, GUO Wenchao², JIANG Weihua¹

1. College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China;
2. Xinjiang Special Environmental Microbiology Laboratory/Institute of Applied Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China;
3. Key Laboratory of Integrated Crop Pest Management in Northwestern Oasis of China,MOARA,P. R. China, Institute of Plant Protection,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China

Received:2020-12-01 Online:2021-04-20 Published:2021-04-25
Correspondence author: JIANG Weihua(1969-), female, native place: Nantong, Jiangsu province, associate professor, master supervisor, research interest: molecular biology and physiological toxicology of insects,(E-mail)jwh@njau.edu.cn
GUO Wenchao(1966-), male, native place: Gucheng, Hebei province, doctoral supervisor. research interest: integrated control of agricultural insects, (E-mail)gwc1966@163.com
Supported by:
Project of National Key R & D Program of China (2017YFD0201800)

摘要/Abstract

摘要： 【目的】 研究监测和检测亚洲玉米螟田间种群对双酰胺类等药剂的抗性,为指导田间合理用药及抗性治理提供依据。【方法】 于2018和2019年,采用人工饲料浸药法,测定新疆乌鲁木齐市、伊宁市、疏勒县、泽普县和昌吉市亚洲玉米螟田间种群对氯虫苯甲酰胺、溴氰虫酰胺、阿立卡(噻虫嗪·高效氯氟氰菊酯)和亮泰(阿维菌素·氯虫苯甲酰胺)的抗性水平。利用定量PCR分析不同田间种群鱼尼丁受体(ryanodine receptor,RyR)基因表达量的变化及利用PCR扩增检测RyR分子突变。【结果】 2018年的测试种群中,与相对种群相比,疏勒种群对氯虫苯甲酰胺为敏感性下降,对阿立卡达到中等水平抗性,抗性倍数(RR)分别为3.67和20.17倍;伊宁种群分别对溴氰虫酰胺、阿立卡产生了15.26和14.10倍的中等水平抗性;泽普种群对阿立卡和亮泰具有中等水平抗性,RR分别为10.97和13.48倍。2019采集的田间种群中,与敏感种群JZ-S相比,除了疏勒种群对溴氰虫酰胺为敏感性降低,其它3个种群(乌鲁木齐市、伊宁市和昌吉市)都产生了低至极高水平的抗性,RR分别为3.61、8.14、96.49和1 429倍。2018年新疆亚洲玉米螟各种群的RyR基因表达量无显著差异,而2019年昌吉、伊宁种群的RyR基因表达量显著升高(4.04、3.86倍);昌吉种群RyR第1 424位谷氨酸(E)替换为天冬氨酸的突变(E1424D),突变频率为30%。【结论】 新疆亚洲玉米螟田间种群对双酰胺类杀虫剂产生低至中等水平抗性,其抗性与鱼尼丁受体基因的表达量增加和突变有关。

关键词: 亚洲玉米螟; 氯虫苯甲酰胺; 溴氰虫酰胺; 抗药性; 鱼尼丁受体; 基因表达; 突变

Abstract: 【Objective】 The study aims to provide a basis for guiding the rational use of insecticides and resistance management for efficient controlling Ostriniafurnacalis through resistance monitoring and detection of field populations to the commonly used insecticides mainly including diamides, acting on the ryanodine receptor (RyR). 【Methods】 In this study, it was monitored for the resistance to insecticides including chlorantraniliprole, cyantraniliprole, aricaandlangat against O.furnacalis from Urumqi (UQ), Shule (SL1,), Yining (YN1) and Zepu (ZP) in 2018 and Urumqi (UQ), Shule(SL2), Yining (YN2) and Changji (CJ) in 2019 in Xinjiang using artificial diet dipping method. Meanwhile, we analyzed and detected expression changes and mutation of ryanodine receptor genes in the populations collected in 2018 and 2019 by quantitative real-time PCR (qPCR) and PCR amplification, respectively. 【Results】 SL1 population had reduced sensitivity to chlorantraniliprole and medium level of resistance to arica with resistance ratios (RR) of 3.67 and 20.17-fold compared to a relatively sensitive population, respectively, YN1 population developed moderate resistance to cyantraniliprole and arica with RR being 15.26 and 14.10-fold respectively, and ZP population had a moderate level of resistance to arica and langat with RR of 10.97 and 13.48-fold, respectively. Among populations collected in 2019, SL2 population showed reduced sensitivity and the rest populations, namely UQ, YN2 and CJ, exhibited low to extremely high level resistance to cyantraniliprole with RR of 3.61, 8.14, 96.49 and 1,429-fold compared with the susceptible population JZ-S, respectively. The qPCR results showed that there was no significant difference in the expression of ryanodine receptor genes among the different field populations collected in 2018, while those in CJ and YN2 populations collected in 2019 increased significantly by 4.04 and 3.86 times compared with the susceptible strain. The gene mutation test results showed that it was detected for a mutation in the ryanodine receptor of CJ population, in which glutamic acid (E) was replaced by aspartic acid (D) at position 1,424 (E1424D) with 30% of the mutation frequency.【Conclusion】 The resistance of the inter-field population of O. furnacalis in Xinjiang to diamides varies from low to medium levels, and its resistance was related to the increase of the expression and mutation of the ryanodine receptor gene.

Key words: Ostriniafurnacalis; chlorantraniliprole; cyantraniliprole; resistance; ryanodine receptor; gene expression; point mutation

中图分类号:

S435.132

支昊宇, 王智慧, 王兴仔, 丁新华, 贾尊尊, 郭文超, 姜卫华. 亚洲玉米螟的抗药性监测及双酰胺类药剂抗性靶标分子检测[J]. 新疆农业科学, 2021, 58(4): 690-699.

ZHI Haoyu, WANG Zhihui, WANG Xingzai, DING Xinhua, JIA Zunzun, GUO Wenchao, JIANG Weihua. Insecticide Resistance Monitoring and Target Molecular Detection of Diamide Insecticides in Field Populations of Ostriniafurnacalis[J]. Xinjiang Agricultural Sciences, 2021, 58(4): 690-699.

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亚洲玉米螟的抗药性监测及双酰胺类药剂抗性靶标分子检测

Insecticide Resistance Monitoring and Target Molecular Detection of Diamide Insecticides in Field Populations of Ostriniafurnacalis

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