Effects of Rhizobium Inoculation Methods on Growth and Nodulation of Multiple Sowing Soybeans

doi:10.6048/j.issn.1001-4330.2022.04.004

Abstract

Abstract:

【Objective】 To explore the effect of different rhizobia inoculation.【Method】 On the growth and nodulation of the multiple sowing soybean, three rhizobia strains (NW5-3, SN7-2 and SMH12) were used as materials, and three inoculation methods of seed fertilizer, application with drip irrigation and seed dressing were carried out on soybean Xindou 8 in southern Xinjiang, the nodule number, nodule dry weight, plant traits, yield and the yield components were measured.【Result】 Inoculation of rhizobia could promote root nodulation and growth of soybean, and the SN7-2 performed better than the SMH12 and NW5-3.Among the inoculation methods, the seed dressing treatment of rhizobia was the most suitable, followed by application with dripping water.Among them, the optimal yield of the seed dressing SN7-2 treatment combination reached 3,575.23 kg/hm², seed dressing SMH12 treatment combination yield second reached 3,376.03 kg/hm².【Conclusion】 Under the experimental conditions, the most suitable match was the combination of seed dressing with rhizobia SN7-2 and SMH-12, followed by drip application with rhizobia SN7-2.

Key words: rhizobium; inoculation mode; multiple sowing soybean; nodulation; growth

摘要：

【目的】研究不同根瘤菌接种方式对复播大豆生长及结瘤效果。【方法】以前期分离、鉴定、纯化的3株根瘤菌菌株为材料,对新疆南疆地区复播大豆新大豆8号进行种肥、随水滴施、拌种等接种方式试验,测定其根瘤数、根瘤干重、植株性状、产量及其构成因素的变化。【结果】接种根瘤菌均能促进大豆根系结瘤和生长发育,根瘤菌SN7-2促进效果最明显,其次是SMH12;根瘤菌接种方式中,根瘤菌拌种处理效果最好,其次是随水滴施。其中拌种SN7-2处理组合产量最佳达到3 575.23 kg/hm²,拌种SMH12处理组合产量次之,达到3 376.03 kg/hm²。【结论】较好的根瘤菌和接种方式组合分别是根瘤菌SN7-2和SMH12拌种,其次是根瘤菌SN7-2滴施。

关键词: 根瘤菌, 接种方式, 复播大豆, 结瘤, 生长

CLC Number:

S565.1
S311

XU Yue, LI Ling, XU Yaxin, HUANG Xingjun, WU Quanzhong, CHEN Guodong, ZHAI Yunlong. Effects of Rhizobium Inoculation Methods on Growth and Nodulation of Multiple Sowing Soybeans[J]. Xinjiang Agricultural Sciences, 2022, 59(4): 810-817.

徐玥, 李玲, 胥雅馨, 黄兴军, 吴全忠, 陈国栋, 翟云龙. 根瘤菌接种方式对复播大豆生长及结瘤的影响[J]. 新疆农业科学, 2022, 59(4): 810-817.

Figures/Tables 3

References 30

[1]	张志民, 董文恒, 田光吉, 等. 提高大豆蛋白质含量的途径与措施[J]. 中国种业, 2017,(3):22-23.
	ZHANG Zhimin, DONG Wenheng, TIAN Guangji, et al. Method and measures to improve the protein content of soybean[J]. China Seed Industry, 2017,(3):22-23.
[2]	张秋磊, 林敏, 平淑珍. 生物固氮及在可持续农业中的应用[J]. 生物技术通报, 2008,(2):1-4.
	ZHANG Qiulei, LIN Min, PING Shuzhen. Biological nitrogen fixation and its application in sustainable agriculture[J]. Biotechnology Bulletin, 2008,(2):1-4.
[3]	李欣欣, 许锐能, 廖红. 大豆共生固氮在农业减肥增效中的贡献及应用潜力[J]. 大豆科学, 2016, 35(4):531-535.
	LI Xinxin, XU Ruineng, LIAO Hong. Contribution and application potential of symbiotic nitrogen fixation in agricultural weight loss and efficiency improvement[J]. Soybean Science, 2016, 35(4):531-535.
[4]	陈文新. 豆科植物根瘤菌-固氮体系在西部大开发中的作用[J]. 草地学报, 2004, 12(1):1-2.
	CHEN Wenxin. Role of legumes rhizobia nitrogen-fixing system in western China development[J]. Journal of Grassland Science, 2004, 12(1):1-2.
[5]	柏宇, 关大伟, 李力, 等. 耐高氮优良大豆根瘤菌株的筛选与鉴定[J]. 大豆科学, 2014, 33(6):861-865.
	BAI Yu, GUAN Dawei, LI Li, et al. Screening and identification of soybean rhizobia strains with high nitrogen tolerance[J]. Soybean Science, 2014, 33(6):861-865.
[6]	Bruno J R A, Robert M B, Segundo U. The success of BNF in soybean in Brazil[J]. Plant and Soil, 2003, 252(1):1-9. DOI URL
[7]	Servicr F. A growing threat down on the farm and glyphosate-the conservationist’s friend[J]. Science, 2007, 316:1114-1117. DOI URL
[8]	Alves B, Boddey R M, Urquiaga S. The success of BNF in soybean in Brazil[J]. Plant and Soil, 2003, 252(1):1-9. DOI URL
[9]	张武, 杨琳, 王紫娟. 生物固氮的研究进展及发展趋势[J]. 云南农业大学学报(自然科学), 2015, 30(5):810-821.
	ZHANG Wu, YANG Lin, WANG Zijuan. Research progress and development trend of biological nitrogen fixation[J]. Journal of Yunnan Agricultural University (Natural Science), 2015, 30(5):810-821.
[10]	马霞, 王丽丽, 李卫军, 等. 不同施氮水平下接种根瘤菌对苜蓿固氮效能及种子生产的影响[J]. 草业学报, 2013, 22(1):95-102.
	MA Xia, WANG Lili, LI Weijun, et al. Effect of rhizobia inoculation on nitrogen fixation efficiency and seed production of alfalfa under different nitrogen levels[J]. Acta Materia Medica Sinica, 2013, 22(1):95-102.
[11]	衡楠楠, 陈远学, 徐开未, 等. 大豆根瘤菌SCAUs8的接种效果、促生性及系统发育研究[J]. 微生物学通报, 2016, 43(8):1708-1714.
	HENG Nannan, CHEN Yuanxue, XU Kaiwei, et al. Study on inoculation effect, pyrogenicity and phylogeny of soybean rhizobia SCAUs8[J]. Chinese Journal of Microbiology, 2016, 43(8):1,708-1,714.
[12]	裴晓峰, 关大伟, 李俊, 等. 耐旱大豆根瘤菌的筛选及其接种效应[J]. 大豆科学, 2012, 31(3):420-424.
	PEI Xiaofeng, GUAN Dawei, LI Jun, et al. Screening and inoculation effect of drought-tolerant soybean rhizobia[J]. Soybean Science, 2012, 31(3):420-424.
[13]	赵念力, 谷维, 张俐俐, 等. 俄罗斯高效大豆根瘤菌肥对大豆主要性状及产量的影响[J]. 江苏农业科学, 2014, 42(1):72-73.
	ZHAO Nianli, GU Wei, ZHANG Lili, et al. Effect of Russian highly efficient soybean rhizobia fertilizer on main traits and yield of soybean[J]. Jiangsu Agricultural Sciences, 2014, 42(1):72-73.
[14]	索炎炎, 张翔, 司贤宗, 等. 氮肥管理与根瘤菌接种模式对花生生长、氮吸收利用及产量的影响[J]. 中国油料作物学报, 2018, 40(6):866-871.
	SUO Yanyan, ZHANG Xiang, SI Xianzong, et al. Effects of nitrogen management and rhizobia inoculation on growth, nitrogen uptake and utilization and yield of peanut[J]. Chinese Journal of Oil Crops, 2012, 40(6):866-871.
[15]	刘庆莉, 王金生, 刘丽君, 等. 大豆根瘤菌剂载体的选择及最佳施用浓度筛选[J]. 大豆科学, 2014, 33(2):207-210.
	LIU Qingli, WANG Jinsheng, LIU Lijun, et al. Selection of soybean rhizobia carrier and optimal application concentration screening[J]. Soybean Science, 2014, 33(2):207-210.
[16]	张红侠, 冯瑞华, 关大伟, 等. 黄土高原地区优良大豆根瘤菌的筛选与接种方式研究[J]. 大豆科学, 2010, 29(6):996-1002.
	ZHANG Hongxia, FENG Ruihua, GUAN Dawei, et al. Screening and inoculation of fine soybean rhizobia in loess plateau region[J]. Soybean Science, 2010, 29(6):996-1002.
[17]	王金生, 丁宁, 吴俊江, 等. 大豆根瘤菌接种效应及竞争结瘤能力分析[J]. 大豆科学, 2017, 36(5):761-767.
	WANG Jinsheng, DING Ning, WU Junjiang, et al. Analysis on the inoculation effect and competitive nodulation capacity of soybean rhizobia[J]. Soybean Science, 2017, 36(5):761-767.
[18]	Bogino P, Banchio E, Bonfiglio C, et al. Competitiveness of a Bradyrhizobium sp.Strain in soils containing indigenous rhizobia[J]. Current Microbiology, 2008, 56:66-72. PMID
[19]	张音, 古丽贤·吐尔逊拜, 苏里坦, 等. 近60 a来新疆不同海拔气候变化的时空特征分析[J]. 干旱区地理, 2019, 42(4):822-829.
	ZHANG Yin, Gulixian Turxunbai, SU Litan, et al. Spatial and temporal characteristics of climate change at different altitudes in Xinjiang in the past 60 years[J]. Arid Land Geography, 2019, 42(4):822-829.
[20]	杜梦迪, 李兰, 罗光明, 等. 气候与土地利用变化对于田绿洲农业耗水特征的影响[J]. 水土保持通报, 2020, 40(3):103-109.
	DU Mengdi, LI Lan, LUO Guangming, et al. Effects of climate and land use change on agricultural water consumption in Yutian Oasis[J]. Bulletin of Soil and Water Conservation, 2020, 40(3):103-109.
[21]	陈传信, 唐江华, 陈佳君, 等. 种植方式对夏大豆鼓粒期叶片光合能力及籽粒灌浆特性的影响[J]. 干旱地区农业研究, 2018, 36(3):101-105.
	CHEN Chuanxin, TANG Jianghua, CHEN Jiajun, et al. Effect of planting patterns on photosynthetic capacity and grain filling characteristics of summer soybean at seed-filling stage[J]. Agricultural Research in the Arid Areas, 2018, 36(3):101-105.
[22]	王鹏辉, 姜昕, 马鸣超, 等. 一株耐干燥大豆根瘤菌菌株的筛选与固氮效果评价[J]. 大豆科学, 2020, 39(1):90-96.
	WANG Penghui, JIANG Xin, MA Mingchao, et al. Screening and nitrogen fixation of a strain resistant to dried soybean rhizobia[J]. Soybean Science, 2020, 39(1):90-96.
[23]	蔡金宏, 李治霖, 王向涛, 等. 接种根瘤菌对于紫花苜蓿抗逆性的研究概况[J]. 黑龙江畜牧兽医, 2020,(9):47-49.
	CAI Jinhong, LI Zhilin, WANG Xiangtao, et al. Research overview of rhizobia inoculation on resistance of Alfalfa[J]. Heilongjiang Animal Husbandry and Veterinary Science, 2020,(9):47-49.
[24]	李涛, 关大伟, 李俊, 等. 黄淮海地区优良大豆根瘤菌株的筛选与接种方式研究[J]. 大豆科学, 2010, 29(4):645-650.
	LI Tao, GUAN Dawei, LI Jun, et al. Screening and inoculation of superior soybean rhizobia strains in the huanghuaihai region[J]. Soybean Science, 2010, 29(4):645-650.
[25]	付萍. 根瘤菌对箭筈豌豆结瘤固氮的影响[J]. 草业科学, 2016, 33(4):584-588.
	FU Ping. Rhizobium influence on nitrogen fixation of nodule arrow Kuo peas[J]. Journal of Pratacultural Science, 2016, 33(4):584-588.
[26]	Mishra P K, Bisht S C, Mishra S, et al. Coincubation of Rhizobium leguminosarum-PR1 with a cold tolerant Pseudomonas sp.improves iron acquisition, nutrient uptake and growth of field pea (Pisum sativum L.)[J]. Journal of Plant Nutrition, 2012, 35(2):243-256. DOI URL
[27]	李艳萍, 张敏, 袁梅, 等. 根瘤菌和复合促生菌对大豆结瘤和生长的影响[J]. 大豆科学, 2017, 36(4):583-591.
	LI Yanping, ZHANG Min, YUAN Mei, et al. Influence of combination of rhizobia and compound growth-promoting bacteria on the nodulation and growth of soybean[J]. Soybean Science, 2017, 36(4):583-591.
[28]	Brear E M, Day D A, Smith P M C. Iron: An essential micronutrient for the legume-rhizobium symbiosis[J]. Frontiers in Plant Science, 2013: 4-359.
[29]	Lanier J E, Jordan D L, Spears J F, et al. Peanut response to inoculation and nitrogen fertilizer[J]. American Society Agronomy, 2005, 97(1):79-84.
[30]	常世豪, 杨青春, 舒文涛, 等. 黄淮海夏大豆品种(系)主要农艺性状的综合性分析[J]. 作物杂志, 2020,(3):62-67.
	CHANG Shihao, YANG Qingchun, SHU Wentao, et al. Comprehensive Analysis of Main Agronomic Traits of Summer Sowing Soybean Varieties (Lines) in Huang-Huai-Hai Region[J]. Crops, 2020,(3):62-67.

接种方式 Inoculation method	根瘤菌 Rhizobia	单株瘤数 Number of nodules per plant (个/株)	根瘤鲜重 Nodule fresh weight per plant (g/株)	根瘤体积 Nodule volume per plant (cm³/株)	根瘤干重 Nodule dry weight per plant (g/株)
种肥 Seed manure	NW5-3	19.79^cC	1.68^bBC	1.54^abAB	0.36^bB
	SN7-2	45.62^aA	3.01^aA	3.02^aA	0.73^aA
	SMH12	34.58^bB	2.63^aAB	1.62^abAB	0.42^bB
	CK	9.83^dC	1.52^bC	0.28^bB	0.08^cC
滴施 Drip irrigation	NW5-3	15.02^bB	0.31^cB	0.34^bB	0.14^bBC
	SN7-2	38.13^aA	1.26^bA	1.28^aA	0.36^aAB
	SMH12	43.79^aA	1.73^aA	1.75^aA	0.43^aA
	CK	4.32^cC	0.14^cB	0.13^bB	0.03^bC
拌种 Seed dressing	NW5-3	26.42^bAB	2.32^aA	1.97^bA	0.63^aA
	SN7-2	58.83^aA	2.61^aA	2.36^aA	0.63^aA
	SMH12	32.41^abAB	2.24^aA	2.14^abA	0.54^aA
	CK	6.42^bB	0.29^bB	0.42^cB	0.05^bB

接种方式 Inoculation method	根瘤菌 Rhizobia	单株瘤数 Number of nodules per plant (个/株)	根瘤鲜重 Nodule fresh weight per plant (g/株)	根瘤体积 Nodule volume per plant (cm³/株)	根瘤干重 Nodule dry weight per plant (g/株)
种肥 Seed manure	NW5-3	19.79^cC	1.68^bBC	1.54^abAB	0.36^bB
	SN7-2	45.62^aA	3.01^aA	3.02^aA	0.73^aA
	SMH12	34.58^bB	2.63^aAB	1.62^abAB	0.42^bB
	CK	9.83^dC	1.52^bC	0.28^bB	0.08^cC
滴施 Drip irrigation	NW5-3	15.02^bB	0.31^cB	0.34^bB	0.14^bBC
	SN7-2	38.13^aA	1.26^bA	1.28^aA	0.36^aAB
	SMH12	43.79^aA	1.73^aA	1.75^aA	0.43^aA
	CK	4.32^cC	0.14^cB	0.13^bB	0.03^bC
拌种 Seed dressing	NW5-3	26.42^bAB	2.32^aA	1.97^bA	0.63^aA
	SN7-2	58.83^aA	2.61^aA	2.36^aA	0.63^aA
	SMH12	32.41^abAB	2.24^aA	2.14^abA	0.54^aA
	CK	6.42^bB	0.29^bB	0.42^cB	0.05^bB

接种方式 Inoculation method	根瘤菌 Rhizobia	株高 Plant height (cm)	茎粗 Stem diameter (cm)	主茎节数 Main stalk pitch number (个/株)	单株荚数 Pods number (个/株)
种肥 Seed manure	NW5-3	44.82^abA	0.49^bA	10.78^aA	17.14^bB
	SN7-2	48.26^aA	0.59^aA	10.82^aA	21.23^aA
	SMH12	41.24^bA	0.55^abA	11.23^aA	22.08^aA
	CK	43.62^abA	0.52^abA	11.08^aA	17.42^bB
滴施 Drip irrigation	NW5-3	57.46^bB	0.72^aA	13.14^aA	33.21^aA
	SN7-2	58.16^bB	0.59^bB	11.82^bA	27.83^bB
	SMH12	54.18^bB	0.63^bAB	12.23^abA	21.43^cC
	CK	67.08^aA	0.65^abAB	12.41^abA	17.83^dD
拌种 Seed dressing	NW5-3	70.53^aA	0.62^aA	13.59^aA	24.42^abA
	SN7-2	57.64^bA	0.55^abA	13.24^aA	30.23^aA
	SMH12	68.28^aA	0.53^bA	13.18^aA	25.32^abA
	CK	55.92^bA	0.59^abA	11.63^bA	20.83^bA

接种方式 Inoculation method	根瘤菌 Rhizobia	株高 Plant height (cm)	茎粗 Stem diameter (cm)	主茎节数 Main stalk pitch number (个/株)	单株荚数 Pods number (个/株)
种肥 Seed manure	NW5-3	44.82^abA	0.49^bA	10.78^aA	17.14^bB
	SN7-2	48.26^aA	0.59^aA	10.82^aA	21.23^aA
	SMH12	41.24^bA	0.55^abA	11.23^aA	22.08^aA
	CK	43.62^abA	0.52^abA	11.08^aA	17.42^bB
滴施 Drip irrigation	NW5-3	57.46^bB	0.72^aA	13.14^aA	33.21^aA
	SN7-2	58.16^bB	0.59^bB	11.82^bA	27.83^bB
	SMH12	54.18^bB	0.63^bAB	12.23^abA	21.43^cC
	CK	67.08^aA	0.65^abAB	12.41^abA	17.83^dD
拌种 Seed dressing	NW5-3	70.53^aA	0.62^aA	13.59^aA	24.42^abA
	SN7-2	57.64^bA	0.55^abA	13.24^aA	30.23^aA
	SMH12	68.28^aA	0.53^bA	13.18^aA	25.32^abA
	CK	55.92^bA	0.59^abA	11.63^bA	20.83^bA

接种方式 Inoculation method	根瘤菌 Rhizobia	单株粒数 Grain number per plant (个/株)	百粒重 100-seed weight(g)	产量 Yield(kg/hm²)
种肥 Seed manure	NW5-3	42.23^bB	13.56^bB	1 817.70^bB
	SN7-2	55.42^aA	16.64^aA	2 776.35^aA
	SMH12	55.16^aA	16.61^aA	2 602.05^aA
	CK	40.38^cC	12.03^cB	1 705.65^cC
滴施 Drip irrigation	NW5-3	59.24^cB	15.53^cB	1 904.85^cC
	SN7-2	78.79^aA	19.63^aA	3 210.03^aA
	SMH12	61.41^bB	18.67^bA	2 380.03^bB
	CK	52.62^dC	13.33^dC	1 869.58^dD
拌种 Seed dressing	NW5-3	55.64^cC	22.47^aA	2 591.68^cB
	SN7-2	82.97^aA	21.97^aA	3 575.23^aA
	SMH12	75.24^bB	22.96^aA	3 376.03^bA
	CK	51.31^dD	20.15^bB	2 151.78^dC