Effects of biopharmaceutical mixture on the control and growth of cotton Verticillium wilt

doi:10.6048/j.issn.1001-4330.2024.07.022

Abstract

Abstract:

【Objective】In order to explore the effects of biological pesticide combinations on cotton Verticillium wilt control and growth, and provide theoretical basis for the healthy growth of cotton plants. 【Methods】A field experiment was conducted in 2022, with Xinluzhong 42 as the experimental variety and drip application as the main method applying 80% ethyl allicin emulsifiable concentrate of 1.5 kg/hm² and potassium phosphite of 15 L/hm² based on a glutathione content of 0.3 kg/hm², they were compounded with antiviral agents (T₁, 0.15 L/hm²), composite microbial fertilizer (T₂, 30 L/hm²), 15% oxamycin (T₃, 2.25 kg/hm²), 5% octambumide acetate (T₄, 1.5 kg/hm²), and 25% pyrazolin (T₅, 2.25 L/hm²), respectively; The effects of different pesticides on cotton disease control, agronomic traits, and yield were analyzed. 【Results】The results showed that each treatment had significant control effects on cotton diseases, promoted cotton growth to a certain extent, and increased cotton plant height, number of fruit branches, and number of buds and bolls. The control effect of T₂ treatment on cotton diseases was 19.99% and 23.34% higher than that of T₁ and T₃ treatment, and the number of bolls in T₁ and T₂ treatment increased by 1.8 and 2.67 compared to CK treatment. The seed cotton yield of T₂ treatment was 6,104.86 kg/hm², which had the highest yield and reached significance than other treatments. 【Conclusion】Under the experimental conditions, drip application of compound microbial fertilizer+80% acetoallin EC+potassium phosphite+glutathione (T₂) can improve the disease control effect to a certain extent, increase the number of buds and bolls per plant, and significantly increase yield.

Key words: cotton; biopharmaceutical; compounding; Verticillium wilt; control effect; growth and development

摘要：

【目的】研究生物药剂复配对棉花黄萎病防治效果及生长发育的影响,为棉花植株健康生长提供理论依据。【方法】于2022年进行大田试验,选用棉花品种为新陆中42号。药剂以80%乙蒜素乳油药液、亚磷酸钾、谷胱甘肽为基础,分别为复配抗病毒专用型助剂(T₁)、复合微生物菌肥(T₂)、15%噁霉灵(T₃)、5%辛菌胺醋酸盐(T₄)、25%吡唑醚菌酯(T₅),分析不同药剂对棉花黄萎病防治效果,农艺性状及产量的影响。【结果】各处理对棉花病害防治效果显著,对棉花生长表现出一定的促进作用,增加棉花株高、果枝数和蕾铃数。其中T₂处理较T₁和T₃处理对棉花黄萎病防治效果提高19.99%、23.34%,T₁、T₂处理较CK处理铃数增加1.8个、2.67个。T₂处理的籽棉产量为6 104.86 kg/hm²,较其他处理产量最高并差异显著。【结论】滴施复合微生物菌肥+80%乙蒜素乳油+亚磷酸钾+谷胱甘肽(T₂)可提高黄萎病防治效果,增加蕾数铃数和单株结铃数,从而显著增加棉花产量。

关键词: 棉花, 生物药剂, 复配, 黄萎病, 防治效果, 生长发育

CLC Number:

S435.62

MA Baihuan, ZHAO Qiang, XIE Jia, XU Kaiyue, REN Ruofei, SONG Xinghu. Effects of biopharmaceutical mixture on the control and growth of cotton Verticillium wilt[J]. Xinjiang Agricultural Sciences, 2024, 61(7): 1748-1756.

马百幻, 赵强, 谢佳, 徐开玥, 任若飞, 宋兴虎. 生物药剂复配对棉花黄萎病防治及生长发育的影响[J]. 新疆农业科学, 2024, 61(7): 1748-1756.

Figures/Tables 8

References 37

[1]	毛树春, 李亚兵, 冯璐, 等. 新疆棉花生产发展问题研究[J]. 农业展望, 2014, 10(11): 43-51.
	MAO Shuchun, LI Yabing, FENG Lu, et al. Study on the development of Xinjiang cotton production[J]. Agricultural Outlook, 2014, 10(11): 43-51.
[2]	杨志恩, 唐建杰, 于学良. 新疆棉花产业现状及对策研究. R农业现代化研究, 2013, 34(3):289-303.
	YANG Zhining, TANG Jianjie, YU Xueliang. Xinjiang cotton industry present situation and countermeasure research[J]. Research of Agricultural Modernization, 2013, 34(3):289-303.
[3]	张一豪, 冯鸿杰, 袁媛, 等. 大丽轮枝菌弱致病力菌株Vd171对棉花黄萎病的诱导免疫作用及机制[J]. 中国农业科学, 2018, 51(6): 1067-1078. DOI
	ZHANG Yihao, FENG Hongjie, YUAN Yuan, et al. Induced immunity effect and mechanism of the weak pathogenicity isolate of Verticillium dahliae Vd171 against Verticillium wilt in cotton[J]. Scientia Agricultura Sinica, 2018, 51(6): 1067-1078. DOI
[4]	刘海洋, 王琦, 王伟, 等. 新疆棉花黄萎病的发生现状及其病原菌的分子鉴定与ISSR分析[J]. 植物保护学报, 2018, 45(6): 1194-1203.
	LIU Haiyang, WANG Qi, WANG Wei, et al. Molecular identification and ISSR analysis ofVerticillium dahliaeand the current status of cottonVerticilliumwilt in Xinjiang[J]. Journal of Plant Protection, 2018, 45(6): 1194-1203.
[5]	刘海洋, 王伟, 张仁福, 等. 新疆主要棉区棉花黄萎病发生概况[J]. 植物保护, 2015, 41(3): 138-142.
	LIU Haiyang, WANG Wei, ZHANG Renfu, et al. Occurrence overviews of cotton Verticillium wilt in Xinjiang[J]. Plant Protection, 2015, 41(3): 138-142.
[6]	章茂林, 夏日照, 廖晓兰. 棉花黄萎病防治方法研究进展[J]. 现代农业科技, 2014,(7): 129-131.
	ZHANG Maolin, XIA Rizhao, LIAO Xiaolan. Research progress of prevention methods on cotton Verticillium wilt[J]. Modern Agricultural Science and Technology, 2014,(7): 129-131.
[7]	朱荷琴, 李志芳, 冯自力, 等. 我国棉花黄萎病研究十年回顾及展望[J]. 棉花学报, 2017, 29(S1): 37-50.
	ZHU Heqin, LI Zhifang, FENG Zili, et al. Review and prospect of cotton verticillium wilt research in China for ten years[J]. Cotton Science, 2017, 29(S1): 37-50.
[8]	万刚. 黄萎病对棉花产量和品质的影响及棉花品种抗病性研究[D]. 石河子: 石河子大学, 2017.
	WAN Gang. The Effectof Verticillium Wilt on Yield and Quality of Cotton and Resistance of Cotton Varieties[D]. Shihezi: Shihezi University, 2017.
[9]	刘政, 孙艳, 张学坤, 等. 木霉菌厚垣孢子制剂对土壤微生物数量和棉花黄萎病的影响[J]. 新疆农业科学, 2015, 52(1): 97-101.
	LIU Zheng, SUN Yan, ZHANG Xuekun, et al. Effect of Trichoderma chlamydospores preparation on amount of soil microorganism and cotton Verticillium wilt[J]. Xinjiang Agricultural Sciences, 2015, 52(1): 97-101.
[10]	孙立娟, 吴国贺, 安承荣, 等. 几种抗病毒病药剂在烤烟生产上的应用研究[J]. 农业与技术, 2019, 39(16): 5-6.
	SUN Lijuan, WU Guohe, AN Chengrong, et al. Study on the application of several antiviral drugs in flue-cured tobacco production[J]. Agriculture and Technology, 2019, 39(16): 5-6.
[11]	彭亚丽, 叶亦心, 胡新喜. 种薯级别和抗病毒药剂处理对秋马铃薯生长与产量的影响[J]. 安徽农学通报, 2021, 27(21): 49-52.
	PENG Yali, YE Yixin, HU Xinxi. The effect of seed potato grades and antiviral treatment on growth and yield of autumn potato[J]. Anhui Agricultural Science Bulletin, 2021, 27(21): 49-52.
[12]	吕博, 孟庆忠, 张成, 等. 复合微生物肥对棉花生长与产量的影响[J]. 新疆农业科学, 2021, 58(6): 1006-1011. DOI
	LYU Bo, MENG Qingzhong, ZHANG Cheng, et al. Effects of compound microbial fertilizer on growth and yield of cotton[J]. Xinjiang Agricultural Sciences, 2021, 58(6): 1006-1011. DOI
[13]	吕博, 孟庆忠, 张成, 等. 微生物菌肥对棉花黄萎病的防治效果研究[J]. 农村经济与科技, 2020, 31(23): 64-65.
	LYU Bo, MENG Qingzhong, ZHANG Cheng, et al. Study on the control effect of microbial bacterial fertilizer on cotton verticillium wilt[J]. Rural Economy and Science-Technology, 2020, 31(23): 64-65.
[14]	马永强. 16%噁霉灵·咯菌腈悬浮剂防治黄瓜枯萎病田间防效试验[J]. 青海农林科技, 2019, (2): 79-81.
	MA Yongqiang. Control effect of 16% Oxemilin·Roxonitrile suspension against cucumber Fusarium wilt in field[J]. Science and Technology of Qinghai Agriculture and Forestry, 2019, (2): 79-81.
[15]	谭放军, 许晓玲, 周程爱, 等. 0.1%噁霉灵颗粒剂防治番茄枯萎病效果初探[J]. 辣椒杂志, 2021, 19(4): 44-47.
	TAN Fangjun, XU Xiaoling, ZHOU Cheng’ai, et al. Effect of 0.1% hymexazol granules on controlling the Fusarium wilt of tomato[J]. Journal of China Capsicum, 2021, 19(4): 44-47.
[16]	曾华兰, 蒋秋平, 叶鹏盛, 等. 种苗处理对麦冬生长的影响及其控病效果研究[J]. 中国农学通报, 2021, 37(6): 137-141. DOI
	ZENG Hualan, JIANG Qiuping, YE Pengsheng, et al. Seedling treatment of Ophiopogon japonicus: effects on growth and disease control efficacy[J]. Chinese Agricultural Science Bulletin, 2021, 37(6): 137-141. DOI
[17]	周艳丽, 雷文军, 李薇, 等. 辛菌胺醋酸盐1.8%水剂防治柑桔溃疡病药效试验[J]. 农药科学与管理, 2014, 35(7): 63-65.
	ZHOU Yanli, LEI Wenjun, LI Wei, et al. Study on the field efficacy of xinjunan acetate 1.8% AS against Citrus canker disease[J]. Pesticide Science and Administration, 2014, 35(7): 63-65.
[18]	章豪, 陈若霞, 吴银良. 辛菌胺对桃的保鲜效果及其风险评估[J]. 食品科技, 2018, 43(6): 265-268.
	ZHANG Hao, CHEN Ruoxia, WU Yinliang. Effects and risk assessment of Xinjunan treatment in the storage of peaches[J]. Food Science and Technology, 2018, 43(6): 265-268.
[19]	杨丽娜, 张亮, 韦永淑, 等. 吡唑醚菌酯及与生物农药复配防治桃枝枯病[J]. 农药, 2022, 61(1): 65-69.
	YANG Lina, ZHANG Liang, WEI Yongshu, et al. Control effects of pyraclostrobin and its mixtures with biopesticides on peach shoot blight[J]. Agrochemicals, 2022, 61(1): 65-69.
[20]	张楠, 徐书举, 曹凤格. 25%吡唑醚菌酯乳油对玉米小斑病病害的防效和增产效果[J]. 农业科技通讯, 2020, (2): 55-58.
	ZHANG Nan, XU Shuju, CAO Fengge. Control effect and yield-increasing effect of 25% pyraclostrobin EC on maize leaf spot disease[J]. Bulletin of Agricultural Science and Technology, 2020, (2): 55-58.
[21]	李慧, 刘保军, 吴琼, 等. 3种生防制剂对棉花红腐病和立枯病的防效评价[J]. 新疆农业科学, 2020, 57(4): 694-704. DOI
	LI Hui, LIU Baojun, WU Qiong, et al. Effect evaluation of control red rot and damping off with three bio-control agents on cotton[J]. Xinjiang Agricultural Sciences, 2020, 57(4): 694-704. DOI
[22]	张京, 孟维实, 孙瑛健, 等. 大豆细菌性斑点病防治药剂筛选[J]. 农药, 2021, 60(9): 687-690.
	ZHANG Jing, MENG Weishi, SUN Yingjian, et al. Screening of fungicides for the control of bacterial blight in soybean[J]. Agrochemicals, 2021, 60(9): 687-690.
[23]	张琪, 赵慧, 刘艾英, 等. 亚磷酸钾防治猕猴桃溃疡病试验研究[J]. 陕西农业科学, 2022, 68(4): 76-78.
	ZHANG Qi, ZHAO Hui, LIU Aiying, et al. Study on control of kiwifruit canker with potassium phosphate[J]. Shaanxi Journal of Agricultural Sciences, 2022, 68(4): 76-78.
[24]	徐继根, 张顺昌, 占红木, 等. 含氨基酸的亚磷酸钾与代森锰锌复配防治葡萄霜霉病的研究[J]. 浙江柑橘, 2020, 37(1): 29-32.
	XU Jigen, ZHANG Shunchang, ZHAN Hongmu, et al. Study on compound control of grape downy mildew with potassium phosphite containing amino acids and mancozeb[J]. Zhejiang Ganju, 2020, 37(1): 29-32.
[25]	李林, 杜卓, 侯雯, 等. 外源谷胱甘肽对低温胁迫下玉米幼苗的影响[J]. 中国农学通报, 2021, 37(27): 16-20. DOI
	LI Lin, DU Zhuo, HOU Wen, et al. Exogenous glutathione: effects on maize seedlings under low temperature stress[J]. Chinese Agricultural Science Bulletin, 2021, 37(27): 16-20. DOI
[26]	李晓云, 王秀峰, 吕乐福, 等. 外源NO对铜胁迫下番茄幼苗根系抗坏血酸-谷胱甘肽循环的影响[J]. 应用生态学报, 2013, 24(4): 1023-1030.
	LI Xiaoyun, WANG Xiufeng, LYU Lefu, et al. Effects of exogenous nitric oxide on ascorbate-glutathione cycle in tomato seedlings roots under copper stress[J]. Chinese Journal of Applied Ecology, 2013, 24(4): 1023-1030. PMID
[27]	马春梅, 田阳青, 赵强, 等. 植物生长调节剂复配对棉花产量的影响[J]. 作物杂志, 2022, (6): 181-185.
	MA Chunmei, TIAN Yangqing, ZHAO Qiang, et al. Effects of plant growth regulator compound on cotton yield[J]. Crops, 2022, (6): 181-185.
[28]	马春梅, 吴雪琴, 李江余, 等. 外源调节剂组合喷施对化学打顶棉花的调控效应[J]. 干旱地区农业研究, 2021, 39(5): 193-198.
	MA Chunmei, WU Xueqin, LI Jiangyu, et al. Regulation effects of different combinations of exogenous substances on chemical topping cotton[J]. Agricultural Research in the Arid Areas, 2021, 39(5): 193-198.
[29]	张特, 李广维, 李可心, 等. 滴施缩节胺对棉花生长发育及产量的影响[J]. 作物杂志, 2022, (4): 124-131.
	ZHANG Te, LI Guangwei, LI Kexin, et al. Effects of DPC through drip irrigation on growth and yield of cotton[J]. Crops, 2022, (4): 124-131.
[30]	王爱玉, 薛超, 杨媛雪, 等. 枯草芽孢杆菌对棉花立枯病和黄萎病的防效评价[J]. 新疆农业科学, 2021, 58(12): 2244-2249. DOI
	WANG Aiyu, XUE Chao, YANG Yuanxue, et al. Evaluation of Control Effect byBacillus subtilison the Damping-off andVerticilliumwilt of Cotton[J]. Xinjiang Agricultural Sciences, 2021, 58(12): 2244-2249. DOI
[31]	潘云平, 李作京, 黄艳萍. 生物农药防治棉花黄萎病试验研究[J]. 现代农业科技, 2008, (11): 122-123.
	PAN Yunping, LI Zuojing, HUANG Yanping. Experimental study on biological pesticides to control cotton verticillium wilt[J]. Anhui Agriculture, 2008, (11): 122-123.
[32]	石磊, 侯振安, 尹飞虎, 等. 随水滴施3种生物菌肥对棉花黄萎病的防治效果[J]. 新疆农垦科技, 2017, 40(2): 37-40.
	SHI Lei, HOU Zhen’an, YIN Feihu, et al. Control effect of three kinds of biological bacterial fertilizers applied with water drops on cotton verticillium wilt[J]. Xinjiang Farm Research of Science and Technology, 2017, 40(2): 37-40.
[33]	刘苹, 张博, 齐军山, 等. 生物有机肥对小麦根腐病的防效及其机理初探[J]. 麦类作物学报, 2019, 39(9): 1132-1137.
	LIU Ping, ZHANG Bo, QI Junshan, et al. Preliminary study on control effect and mechanism of bioorganic fertilizers on wheat root rot[J]. Journal of Triticeae Crops, 2019, 39(9): 1132-1137.
[34]	刘放. 烟草普通花叶病毒生防菌的筛选及抗病毒机理研究[D]. 长沙: 湖南农业大学, 2020.
	LIU Fang. Screening and antiviral mechanism of biocontrol bacteria against tobacco common mosaic virus[D]. Changsha: Hunan Agricultural University, 2020.
[35]	何雪玲. 芹菜定植前药剂蘸根防治根腐病效果试验[J]. 西北园艺(综合), 2022, (3): 60-61.
	HE Xueling. Experiment on the effect of chemical dipping in roots to control root rot of celery before planting[J]. Northwest Horticulture, 2022, (3): 60-61.
[36]	柳自清, 张博然, 顾爱星. 几种杀菌剂对棉花枯萎病的田间防效评价[J]. 农业与技术, 2021, 41(9): 21-26.
	LIU Ziqing, ZHANG Boran, GU Aixing. Evaluation of field control effect of several fungicides on cotton Fusarium wilt[J]. Agriculture and Technology, 2021, 41(9): 21-26.
[37]	吕宁, 石磊, 刘海燕, 等. 生物药剂滴施对棉花黄萎病及根际土壤微生物数量和多样性的影响[J]. 应用生态学报, 2019, 30(2): 602-614.
	LYU Ning, SHI Lei, LIU Haiyan, et al. Effects of biological agent dripping on cotton Verticillium wilt and rhizosphere soil microorganism[J]. Chinese Journal of Applied Ecology, 2019, 30(2): 602-614.

试验编号 Test number	试验处理 Test treatments	剂量Dosage (kg/hm²或L/hm²)
T₁	抗病毒专用型助剂+80%乙蒜素乳油+亚磷酸钾+谷胱甘肽	0.15 L/hm²+1.5 kg/hm²+15 L/hm²+0.3 kg/hm²
T₂	复合微生物菌肥+80%乙蒜素乳油+亚磷酸钾+谷胱甘肽	30 L/hm²+1.5 kg/hm²+1.5 L/hm²+0.3 kg/hm²
T₃	15%噁霉灵+80%乙蒜素乳油+亚磷酸钾+谷胱甘肽	2.25 kg/hm²+1.5 kg/hm²+1.5 L/hm²+0.3 kg/hm²
T₄	5%辛菌胺醋酸盐+80%乙蒜素乳油+亚磷酸钾+谷胱甘肽	1.5 kg/hm²+1.5 kg/hm²+1.5 L/hm²+0.3 kg/hm²
T₅	25%吡唑醚菌酯+80%乙蒜素乳油+亚磷酸钾+谷胱甘肽	2.25 L/hm²+1.5 kg/hm²+1.5 L/hm²+0.3 kg/hm²
CK	清水	/

试验编号 Test number	试验处理 Test treatments	剂量Dosage (kg/hm²或L/hm²)
T₁	抗病毒专用型助剂+80%乙蒜素乳油+亚磷酸钾+谷胱甘肽	0.15 L/hm²+1.5 kg/hm²+15 L/hm²+0.3 kg/hm²
T₂	复合微生物菌肥+80%乙蒜素乳油+亚磷酸钾+谷胱甘肽	30 L/hm²+1.5 kg/hm²+1.5 L/hm²+0.3 kg/hm²
T₃	15%噁霉灵+80%乙蒜素乳油+亚磷酸钾+谷胱甘肽	2.25 kg/hm²+1.5 kg/hm²+1.5 L/hm²+0.3 kg/hm²
T₄	5%辛菌胺醋酸盐+80%乙蒜素乳油+亚磷酸钾+谷胱甘肽	1.5 kg/hm²+1.5 kg/hm²+1.5 L/hm²+0.3 kg/hm²
T₅	25%吡唑醚菌酯+80%乙蒜素乳油+亚磷酸钾+谷胱甘肽	2.25 L/hm²+1.5 kg/hm²+1.5 L/hm²+0.3 kg/hm²
CK	清水	/

级别 Grade	分级标准 Test processing	级值 Value
0级 Level 0	健株,整个植株叶片无病症	0
1级 Level 1	病株叶片有25%以下显病状,叶片主脉间产生淡黄色不规则病斑	1
2级 Level 2	病株叶片有25%-50%显病状,病斑颜色大部分变为黄色和黄褐色,叶片边缘有卷枯	2
3级 Level 3	病株叶片有50%以上显病状,病斑颜色为黄褐色,叶片边缘卷枯,有少数叶片掉落	3
4级 Level 4	病株叶脱落成光杆及植株死亡,或是出现急性死亡症状	4

级别 Grade	分级标准 Test processing	级值 Value
0级 Level 0	健株,整个植株叶片无病症	0
1级 Level 1	病株叶片有25%以下显病状,叶片主脉间产生淡黄色不规则病斑	1
2级 Level 2	病株叶片有25%-50%显病状,病斑颜色大部分变为黄色和黄褐色,叶片边缘有卷枯	2
3级 Level 3	病株叶片有50%以上显病状,病斑颜色为黄褐色,叶片边缘卷枯,有少数叶片掉落	3
4级 Level 4	病株叶脱落成光杆及植株死亡,或是出现急性死亡症状	4

处理 Treatments	调查株数 Investigated plants	药后11 d 11 days after spraying fungicides		药后21 d 21 days after spraying fungicides		药后30 d 30 days after spraying fungicides
处理 Treatments	调查株数 Investigated plants	发病率 Incidence (%)	病情指数 Disease index	发病率 Incidence (%)	病情指数 Disease index	发病率 Incidence (%)	病情指数 Disease index
T₁	300	11.33^b	3.75±0.90^cd	20.33^c	8.50±1.75^c	31.67^cd	15.00±1.80^c
T₂	300	8.33^c	2.75±0.25^d	19.33^c	8.25±1.80^c	28.67^d	14.75±0.66^c
T₃	300	12.33^b	3.92±0.14^cd	22.67^bc	9.25±1.09^bc	32.00^cd	15.50±0.25^bc
T₄	300	14.00^b	5.50±0.43^b	26.33^b	12.00±0.43^b	34.33^bc	16.83±0.52^b
T₅	300	12.00^b	4.50±0.90^bc	24.00^bc	11.08±0.63^bc	38.33^b	16.33±0.38^bc
CK	300	21.67^a	7.75±1.09^a	38.00^a	17.58±3.21^a	45.33^a	23.08±0.52^a