Xinjiang Agricultural Sciences ›› 2023, Vol. 60 ›› Issue (9): 2109-2119.DOI: 10.6048/j.issn.1001-4330.2023.09.004
• Crop Genetics and Breeding·Germplasm Resources·Molecular Genetics·Soil Fertilizer • Previous Articles Next Articles
WANG Hui1(), GUO Jincheng1, SONG Jia1, ZHANG Tingjun2(), He Liangrong1()
Received:
2022-11-12
Online:
2023-09-20
Published:
2023-09-19
Correspondence author:
HE Liangrong(1970-), female, Hubei, master, research direction: research and innovation of crop germplasm resources,(E-mail)Supported by:
王辉1(), 郭金成1, 宋佳1, 张庭军2(), 何良荣1()
通讯作者:
何良荣(1970-),女,湖北人,副教授,博士,硕士生导师,研究方向为棉花种质资源,(E-mail)作者简介:
王辉(1993-),男,河南人,硕士研究生,研究方向为作物种质资源,(E-mail)347488940@qq.com
基金资助:
CLC Number:
WANG Hui, GUO Jincheng, SONG Jia, ZHANG Tingjun, He Liangrong. Physiological and biochemical analysis of transgenic offspring of upland cotton GhCIPK6 under high temperature Stress[J]. Xinjiang Agricultural Sciences, 2023, 60(9): 2109-2119.
王辉, 郭金成, 宋佳, 张庭军, 何良荣. 高温胁迫下陆地棉GhCIPK6转基因后代生理生化分析[J]. 新疆农业科学, 2023, 60(9): 2109-2119.
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Fig. 1 Comparison of upland cotton leaves Note:AB represent 4h positive growth temperature and high temperature seedlings respectively, and C represents 48h seedling leaves
因子 Factors | 成分 Principal components | |||
---|---|---|---|---|
F1 | F2 | F3 | F4 | |
丙二醛 MDA | -0.324 | 0.207 | 0.726 | -0.41 |
净光合速率 Pn | 0.798 | 0.264 | 0.452 | 0.178 |
气孔导度 Gs | 0.9 | -0.022 | 0.251 | 0.307 |
胞间CO2浓度 Ci | 0.843 | -0.05 | -0.086 | -0.125 |
蒸腾速率Tr | 0.857 | -0.025 | 0.313 | 0.375 |
叶绿素相对值SPAD | 0.816 | 0.074 | 0.057 | -0.465 |
超氧化物歧化酶SOD | 0.573 | 0.559 | -0.318 | 0.276 |
过氧化物酶POD | -0.637 | -0.251 | 0.619 | 0.179 |
可溶性蛋白Souble protein | -0.074 | -0.827 | 0.234 | -0.155 |
脯氨酸Pro | -0.635 | -0.434 | 0.113 | 0.449 |
脱落酸 ABA | 0.481 | -0.566 | -0.374 | -0.136 |
生长素IAA | 0.013 | 0.686 | 0.47 | -0.277 |
茉莉酸JA | -0.604 | 0.591 | -0.47 | -0.116 |
水杨酸SA | -0.476 | 0.666 | 0.08 | 0.333 |
方差贡献率 Variance contribution rate(%) | 40.182 | 21.09 | 14.601 | 8.714 |
累计贡献率 Cumulative contribution rate(%) | 40.182 | 61.272 | 75.873 | 84.587 |
Tab.1 Factor loading matrix of main composition and principal component eigenvalues
因子 Factors | 成分 Principal components | |||
---|---|---|---|---|
F1 | F2 | F3 | F4 | |
丙二醛 MDA | -0.324 | 0.207 | 0.726 | -0.41 |
净光合速率 Pn | 0.798 | 0.264 | 0.452 | 0.178 |
气孔导度 Gs | 0.9 | -0.022 | 0.251 | 0.307 |
胞间CO2浓度 Ci | 0.843 | -0.05 | -0.086 | -0.125 |
蒸腾速率Tr | 0.857 | -0.025 | 0.313 | 0.375 |
叶绿素相对值SPAD | 0.816 | 0.074 | 0.057 | -0.465 |
超氧化物歧化酶SOD | 0.573 | 0.559 | -0.318 | 0.276 |
过氧化物酶POD | -0.637 | -0.251 | 0.619 | 0.179 |
可溶性蛋白Souble protein | -0.074 | -0.827 | 0.234 | -0.155 |
脯氨酸Pro | -0.635 | -0.434 | 0.113 | 0.449 |
脱落酸 ABA | 0.481 | -0.566 | -0.374 | -0.136 |
生长素IAA | 0.013 | 0.686 | 0.47 | -0.277 |
茉莉酸JA | -0.604 | 0.591 | -0.47 | -0.116 |
水杨酸SA | -0.476 | 0.666 | 0.08 | 0.333 |
方差贡献率 Variance contribution rate(%) | 40.182 | 21.09 | 14.601 | 8.714 |
累计贡献率 Cumulative contribution rate(%) | 40.182 | 61.272 | 75.873 | 84.587 |
材料 Materials | 时间 Time | F1 | F2 | F3 | F4 | 综合得分 Comprehensive score | 综合排序 Comprehensive ranking |
---|---|---|---|---|---|---|---|
WT | 4 | 2.912 | -0.330 | 0.406 | -1.114 | 0.149 | 3 |
12 | -4.233 | 1.175 | -1.983 | -0.778 | -0.253 | 12 | |
24 | 0.178 | 0.960 | 2.404 | 0.207 | 0.090 | 5 | |
48 | 2.309 | 1.532 | -1.393 | 0.275 | 0.150 | 2 | |
Ri | 4 | 2.837 | -0.822 | -1.246 | -1.425 | 0.092 | 4 |
12 | -2.519 | 1.746 | -0.114 | -1.211 | -0.107 | 10 | |
24 | -1.572 | 1.307 | 2.498 | 0.276 | 0.005 | 6 | |
48 | 2.482 | 2.150 | -0.224 | 1.069 | 0.212 | 1 | |
OE | 4 | 0.808 | -2.588 | 0.587 | -0.524 | -0.025 | 8 |
12 | -2.471 | -2.673 | -0.263 | 0.366 | -0.219 | 11 | |
24 | -0.569 | -1.863 | 0.708 | 0.372 | -0.068 | 9 | |
48 | -0.162 | -0.593 | -1.378 | 2.488 | -0.024 | 7 |
Tab.2 Principal scores and comprehensive evaluation results
材料 Materials | 时间 Time | F1 | F2 | F3 | F4 | 综合得分 Comprehensive score | 综合排序 Comprehensive ranking |
---|---|---|---|---|---|---|---|
WT | 4 | 2.912 | -0.330 | 0.406 | -1.114 | 0.149 | 3 |
12 | -4.233 | 1.175 | -1.983 | -0.778 | -0.253 | 12 | |
24 | 0.178 | 0.960 | 2.404 | 0.207 | 0.090 | 5 | |
48 | 2.309 | 1.532 | -1.393 | 0.275 | 0.150 | 2 | |
Ri | 4 | 2.837 | -0.822 | -1.246 | -1.425 | 0.092 | 4 |
12 | -2.519 | 1.746 | -0.114 | -1.211 | -0.107 | 10 | |
24 | -1.572 | 1.307 | 2.498 | 0.276 | 0.005 | 6 | |
48 | 2.482 | 2.150 | -0.224 | 1.069 | 0.212 | 1 | |
OE | 4 | 0.808 | -2.588 | 0.587 | -0.524 | -0.025 | 8 |
12 | -2.471 | -2.673 | -0.263 | 0.366 | -0.219 | 11 | |
24 | -0.569 | -1.863 | 0.708 | 0.372 | -0.068 | 9 | |
48 | -0.162 | -0.593 | -1.378 | 2.488 | -0.024 | 7 |
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