Xinjiang Agricultural Sciences ›› 2021, Vol. 58 ›› Issue (10): 1947-1953.DOI: 10.6048/j.issn.1001-4330.2021.10.023
• Prataculture·Agricultural Product Processing Engineering·Agricultural Eeconomy • Previous Articles Next Articles
Xinxin HUANG(), Jiahao LI, Xiaobin LI(), Hongkun LI, Yiqi JIA, Lixin MA, Chao LI, Linjiao HE
Received:
2020-06-07
Online:
2021-10-20
Published:
2021-10-26
Correspondence author:
Xiaobin LI
Supported by:
黄新新(), 李佳豪, 李晓斌(), 李宏坤, 贾怡琪, 马利鑫, 李超, 贺林娇
通讯作者:
李晓斌
作者简介:
黄新新(1997-),男,湖北丹江口人,硕士研究生,研究方向为动物营养与饲料科学,(E-mail) 1196129362@qq.com
基金资助:
CLC Number:
Xinxin HUANG, Jiahao LI, Xiaobin LI, Hongkun LI, Yiqi JIA, Lixin MA, Chao LI, Linjiao HE. Effects of Lactobacillus rhamnoides on the Diversity of Fecal Flora in Late Pregnant Mice[J]. Xinjiang Agricultural Sciences, 2021, 58(10): 1947-1953.
黄新新, 李佳豪, 李晓斌, 李宏坤, 贾怡琪, 马利鑫, 李超, 贺林娇. 灌喂鼠李糖乳杆菌对妊娠后期小鼠粪便菌群多样性影响[J]. 新疆农业科学, 2021, 58(10): 1947-1953.
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原料 Ingredients | 含量 Content | 营养水平 Nutrient levels | 含量 Content |
---|---|---|---|
小麦粉 Wheat flour | 32.00 | 干物质DA | 92.48 |
玉米 Corn | 32.00 | 有机物OM | 90.67 |
豆粕 Soybean meal | 24.00 | 粗蛋白质CP | 16.56 |
鱼粉 Fish meal | 8.00 | 中性洗涤纤维NDF | 16.95 |
葵饼 Sunflower meal | 8.00 | 酸性洗涤纤维ADF | 7.50 |
肉骨粉 Meat and bone meal | 4.00 | 钙Ca | 1.69 |
食盐 NaCl | 1.00 | 磷P | 0.12 |
磷酸氢钙 CaHPO4 | 0.50 | ||
石粉 Limestone | 0.48 | ||
多种维生素 Multi vitamin | 0.02 | ||
合计 Total | 100 |
Table 1 Composition and nutrient levels of diet (Air-dry basis,%)
原料 Ingredients | 含量 Content | 营养水平 Nutrient levels | 含量 Content |
---|---|---|---|
小麦粉 Wheat flour | 32.00 | 干物质DA | 92.48 |
玉米 Corn | 32.00 | 有机物OM | 90.67 |
豆粕 Soybean meal | 24.00 | 粗蛋白质CP | 16.56 |
鱼粉 Fish meal | 8.00 | 中性洗涤纤维NDF | 16.95 |
葵饼 Sunflower meal | 8.00 | 酸性洗涤纤维ADF | 7.50 |
肉骨粉 Meat and bone meal | 4.00 | 钙Ca | 1.69 |
食盐 NaCl | 1.00 | 磷P | 0.12 |
磷酸氢钙 CaHPO4 | 0.50 | ||
石粉 Limestone | 0.48 | ||
多种维生素 Multi vitamin | 0.02 | ||
合计 Total | 100 |
项目 Items | 对照组 Control group | 试验组 Test group |
---|---|---|
Shannon | 5.65±0.27 | 4.90±1.03 |
Simpson | 0.96±0.01 | 0.90±0.09 |
Chao1 | 440.95±16.30 | 444.77±31.18 |
ACE | 448.81±14.09 | 448.56±29.04 |
OTUs (个) | 385 | 425 |
共有OTUs (个) | 304 | |
特有OTUs (个) | 71 | 111 |
Table 2 Fecal flora α-diversity
项目 Items | 对照组 Control group | 试验组 Test group |
---|---|---|
Shannon | 5.65±0.27 | 4.90±1.03 |
Simpson | 0.96±0.01 | 0.90±0.09 |
Chao1 | 440.95±16.30 | 444.77±31.18 |
ACE | 448.81±14.09 | 448.56±29.04 |
OTUs (个) | 385 | 425 |
共有OTUs (个) | 304 | |
特有OTUs (个) | 71 | 111 |
项目 Items | 对照组 Control group | 试验组 Test group |
---|---|---|
厚壁菌门 Firmicutes | 51.35±2.25b | 61.68±3.14a |
拟杆菌门 Bacteroidetes | 43.00±2.01a | 33.56±1.89b |
变形菌门 Proteobacteria | 2.60±0.01a | 1.92±0.02b |
放线菌门 Actinobacteria | 0.20±0.01 | 0.36±0.02 |
Unide.pngied_Bacteria | 0.41±0.11 | 0.21±0.04 |
软壁菌门 Tenericutes | 0.36±0.10 | 0.16±0.02 |
脱铁杆菌门 Deferribacteres | 0.02±0.01 | 0.05±0.01 |
蓝细菌 Cyanobacteria | 0.01±0.00 | 0.03±0.00 |
酸杆菌门 Acidobacteria | 0.02±0.00 | 0.01±0.01 |
Table 3 Abundance of phylum level species (%)
项目 Items | 对照组 Control group | 试验组 Test group |
---|---|---|
厚壁菌门 Firmicutes | 51.35±2.25b | 61.68±3.14a |
拟杆菌门 Bacteroidetes | 43.00±2.01a | 33.56±1.89b |
变形菌门 Proteobacteria | 2.60±0.01a | 1.92±0.02b |
放线菌门 Actinobacteria | 0.20±0.01 | 0.36±0.02 |
Unide.pngied_Bacteria | 0.41±0.11 | 0.21±0.04 |
软壁菌门 Tenericutes | 0.36±0.10 | 0.16±0.02 |
脱铁杆菌门 Deferribacteres | 0.02±0.01 | 0.05±0.01 |
蓝细菌 Cyanobacteria | 0.01±0.00 | 0.03±0.00 |
酸杆菌门 Acidobacteria | 0.02±0.00 | 0.01±0.01 |
项目 Items | 对照组 Control group | 试验组 Test group |
---|---|---|
毛螺菌科Lachnospiraceae | 21.89±3.21b | 27.05±3.33a |
Muribaculaceae | 36.57±3.45 | 34.14±3.26 |
乳酸细菌科 Lactobacillaceae | 10.41±2.16b | 15.64±2.11a |
韦荣球菌科 Erysipelotrichaceae | 5.06±1.35b | 12.04±3.01a |
不明梭菌属 Unide.pngied_Clostridiales | 8.77±2.04 | 6.97±1.96 |
脱硫弧菌科 Desulfovibrionaceae | 2.42±.1.10 | 2.84±1.03 |
理研菌科 Rikenellaceae | 2.99±1.14 | 0.33±0.06 |
普雷沃氏菌科 Prevotellaceae | 1.96±0.12 | 0.81±0.31 |
瘤胃菌科 Ruminococcaceae | 1.63±0.66 | 1.51±0.53 |
Table 4 Abundance of family level species (%)
项目 Items | 对照组 Control group | 试验组 Test group |
---|---|---|
毛螺菌科Lachnospiraceae | 21.89±3.21b | 27.05±3.33a |
Muribaculaceae | 36.57±3.45 | 34.14±3.26 |
乳酸细菌科 Lactobacillaceae | 10.41±2.16b | 15.64±2.11a |
韦荣球菌科 Erysipelotrichaceae | 5.06±1.35b | 12.04±3.01a |
不明梭菌属 Unide.pngied_Clostridiales | 8.77±2.04 | 6.97±1.96 |
脱硫弧菌科 Desulfovibrionaceae | 2.42±.1.10 | 2.84±1.03 |
理研菌科 Rikenellaceae | 2.99±1.14 | 0.33±0.06 |
普雷沃氏菌科 Prevotellaceae | 1.96±0.12 | 0.81±0.31 |
瘤胃菌科 Ruminococcaceae | 1.63±0.66 | 1.51±0.53 |
项目 Items | 对照组 Control group | 试验组 Test group |
---|---|---|
乳酸杆菌属 Lactobacillus | 10.41±2.33b | 15.64±3.65a |
未确定毛螺菌科属 Unide.pngied_Lachnospiraceae | 5.47±1.98b | 12.84±3.37a |
Candidatus_Arthromitus | 8.70±2.97 | 6.93±1.31 |
Ileibacterium | 0.05±0.00 | 7.12±2.16 |
Lachnoclostridium | 1.33±0.18b | 1.95±0.29a |
Faecalibaculum | 0.04±0.01 | 0.44±0.00 |
脱硫弧菌属 Desulfovibrio | 2.39±0.14 | 2.83±0.26 |
Turicibacter | 4.51±1.13 | 3.93±1.99 |
Alistipes | 1.36±0.35 | 0.19±0.09 |
罗氏菌属 Roseburia | 2.69±0.83 | 0.78±0.22 |
Table 5 Abundance of species of genus level (%)
项目 Items | 对照组 Control group | 试验组 Test group |
---|---|---|
乳酸杆菌属 Lactobacillus | 10.41±2.33b | 15.64±3.65a |
未确定毛螺菌科属 Unide.pngied_Lachnospiraceae | 5.47±1.98b | 12.84±3.37a |
Candidatus_Arthromitus | 8.70±2.97 | 6.93±1.31 |
Ileibacterium | 0.05±0.00 | 7.12±2.16 |
Lachnoclostridium | 1.33±0.18b | 1.95±0.29a |
Faecalibaculum | 0.04±0.01 | 0.44±0.00 |
脱硫弧菌属 Desulfovibrio | 2.39±0.14 | 2.83±0.26 |
Turicibacter | 4.51±1.13 | 3.93±1.99 |
Alistipes | 1.36±0.35 | 0.19±0.09 |
罗氏菌属 Roseburia | 2.69±0.83 | 0.78±0.22 |
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