Xinjiang Agricultural Sciences ›› 2023, Vol. 60 ›› Issue (10): 2501-2508.DOI: 10.6048/j.issn.1001-4330.2023.10.020
• Plant Protection·Microbes·Soil Fertilizer • Previous Articles Next Articles
LUO Ying1(), Nurziya Yalimaimaiti1, JIA Wenjie1, LIU Jieying2, JIA Peisong1()
Received:
2023-01-21
Online:
2023-10-20
Published:
2023-11-01
Correspondence author:
JIA Pei-song(1984-), male, Hebei province, Associate researcher, Research field: Edible fungi, (E-mail)Supported by:
罗影1(), 努尔孜亚·亚力买买提1, 贾文捷1, 刘洁莹2, 贾培松1()
通讯作者:
贾培松(1984-),男,河北人,副研究员,硕士,研究方向为食用菌, (E-mail)作者简介:
罗影(1988-),女,河北人,副研究员,硕士,研究方向为食用菌,(E-mail)ly19880219@126.com
基金资助:
CLC Number:
LUO Ying, Nurziya Yalimaimaiti, JIA Wenjie, LIU Jieying, JIA Peisong. Identification and genetic diversity of wild Macrolepiota in Xinjiang[J]. Xinjiang Agricultural Sciences, 2023, 60(10): 2501-2508.
罗影, 努尔孜亚·亚力买买提, 贾文捷, 刘洁莹, 贾培松. 新疆野生大环柄菇的分子鉴定与遗传多样性分析[J]. 新疆农业科学, 2023, 60(10): 2501-2508.
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URL: http://www.xjnykx.com/EN/10.6048/j.issn.1001-4330.2023.10.020
序号 No. | 引物 编号 Primer No. | 引物序列 Primer sequence (5'-3') | 退火 温度 Annea- ling temper- ature (℃) |
---|---|---|---|
1 | P2 | GTGACACACACACAC | 56 |
2 | P4 | GGATGCAACACACACACAC | 55 |
3 | P5 | CGTGTGTGTGTGTGT | 48 |
4 | P20 | ACACACACACACACACCTG | 52 |
5 | P21 | AGCAGCAGCAGCAGCAGCG | 56 |
6 | P22 | AAGAAGAAGAAGAAGAAGC | 50 |
7 | P25 | GAGAGAGAGAGAGAGACC | 56 |
8 | P26 | CACCACACACACACACA | 50 |
9 | P807 | AGAGAGAGAGAGAGAGT | 55 |
10 | P864 | ATGATGATGATGATGATG | 50 |
Tab.1 Sequence of ISSR primers used in the study
序号 No. | 引物 编号 Primer No. | 引物序列 Primer sequence (5'-3') | 退火 温度 Annea- ling temper- ature (℃) |
---|---|---|---|
1 | P2 | GTGACACACACACAC | 56 |
2 | P4 | GGATGCAACACACACACAC | 55 |
3 | P5 | CGTGTGTGTGTGTGT | 48 |
4 | P20 | ACACACACACACACACCTG | 52 |
5 | P21 | AGCAGCAGCAGCAGCAGCG | 56 |
6 | P22 | AAGAAGAAGAAGAAGAAGC | 50 |
7 | P25 | GAGAGAGAGAGAGAGACC | 56 |
8 | P26 | CACCACACACACACACA | 50 |
9 | P807 | AGAGAGAGAGAGAGAGT | 55 |
10 | P864 | ATGATGATGATGATGATG | 50 |
序号 No. | 菌株 Strain | 采集地 | 菌落形态 Colony morphology | 菌丝长势 The growth potentia | 菌丝生长速度 Growth vigor(mm/d) |
---|---|---|---|---|---|
1 | HBG001 | 哈巴河县 | 菌丝浓密,边缘整齐 | +++ | 1.49±0.08ef |
2 | HBG002 | 哈巴河县 | 菌丝较浓密,边缘整齐 | +++ | 1.99±0.09ab |
3 | HBG005 | 哈巴河县 | 菌丝浓密,边缘不整齐 | + | 1.44±0.03f |
4 | HBG006 | 哈巴河县 | 菌丝浓密,边缘不整齐 | ++ | 1.94±0.18bc |
5 | HBG008 | 特克斯县 | 菌丝浓密,边缘不整齐 | ++ | 1.69±0.33de |
6 | HBG009 | 特克斯县 | 菌丝较浓密,边缘不整齐 | ++ | 1.37±0.19fgh |
7 | HBG010 | 哈巴河县 | 菌丝浓密,边缘不整齐 | ++ | 1.42±0.08fg |
8 | HBG011 | 哈巴河县 | 菌丝浓密,边缘不整齐 | ++ | 1.47±0.15ef |
9 | HBG012 | 哈巴河县 | 菌丝浓密,边缘不整齐 | ++ | 1.13±0.10 |
10 | HBG013 | 哈巴河县 | 菌丝浓密,边缘不整齐 | ++ | 1.20±0.05ghi |
11 | HBG014 | 哈巴河县 | 菌丝浓密,边缘不整齐 | ++ | 1.09±0.12 |
12 | HBG015 | 哈巴河县 | 菌丝浓密,边缘不整齐 | ++ | 1.14±0.06hi |
13 | HBG016 | 哈巴河县 | 菌丝浓密,边缘整齐 | ++++ | 2.17±0.09a |
14 | HBG017 | 哈巴河县 | 菌丝浓密,边缘不整齐 | ++ | 1.21±0.10ghi |
15 | HBG018 | 哈巴河县 | 菌丝浓密,边缘不整齐 | ++ | 1.19±0.06ghi |
16 | HBG019 | 哈巴河县 | 菌丝浓密,边缘不整齐 | +++ | 1.74±0.22cd |
17 | HBG020 | 哈巴河县 | 菌丝浓密,边缘不整齐 | ++ | 1.04±0.18 |
Tab.2 The description of culture characteristics for Macrolepiota excoriata strains
序号 No. | 菌株 Strain | 采集地 | 菌落形态 Colony morphology | 菌丝长势 The growth potentia | 菌丝生长速度 Growth vigor(mm/d) |
---|---|---|---|---|---|
1 | HBG001 | 哈巴河县 | 菌丝浓密,边缘整齐 | +++ | 1.49±0.08ef |
2 | HBG002 | 哈巴河县 | 菌丝较浓密,边缘整齐 | +++ | 1.99±0.09ab |
3 | HBG005 | 哈巴河县 | 菌丝浓密,边缘不整齐 | + | 1.44±0.03f |
4 | HBG006 | 哈巴河县 | 菌丝浓密,边缘不整齐 | ++ | 1.94±0.18bc |
5 | HBG008 | 特克斯县 | 菌丝浓密,边缘不整齐 | ++ | 1.69±0.33de |
6 | HBG009 | 特克斯县 | 菌丝较浓密,边缘不整齐 | ++ | 1.37±0.19fgh |
7 | HBG010 | 哈巴河县 | 菌丝浓密,边缘不整齐 | ++ | 1.42±0.08fg |
8 | HBG011 | 哈巴河县 | 菌丝浓密,边缘不整齐 | ++ | 1.47±0.15ef |
9 | HBG012 | 哈巴河县 | 菌丝浓密,边缘不整齐 | ++ | 1.13±0.10 |
10 | HBG013 | 哈巴河县 | 菌丝浓密,边缘不整齐 | ++ | 1.20±0.05ghi |
11 | HBG014 | 哈巴河县 | 菌丝浓密,边缘不整齐 | ++ | 1.09±0.12 |
12 | HBG015 | 哈巴河县 | 菌丝浓密,边缘不整齐 | ++ | 1.14±0.06hi |
13 | HBG016 | 哈巴河县 | 菌丝浓密,边缘整齐 | ++++ | 2.17±0.09a |
14 | HBG017 | 哈巴河县 | 菌丝浓密,边缘不整齐 | ++ | 1.21±0.10ghi |
15 | HBG018 | 哈巴河县 | 菌丝浓密,边缘不整齐 | ++ | 1.19±0.06ghi |
16 | HBG019 | 哈巴河县 | 菌丝浓密,边缘不整齐 | +++ | 1.74±0.22cd |
17 | HBG020 | 哈巴河县 | 菌丝浓密,边缘不整齐 | ++ | 1.04±0.18 |
序号 No. | 引物编号 Primer No. | 扩增条带数 No.of bands(n) | 多态性 条带数 No.of polymorphic bands (k) | 多态位 点比率 Fraction of polymorphic ( p /100% ) |
---|---|---|---|---|
1 | P2 | 7 | 3 | 42.86 |
2 | P4 | 14 | 11 | 78.57 |
3 | P5 | 13 | 9 | 69.23 |
4 | P20 | 11 | 8 | 72.73 |
5 | P21 | 9 | 3 | 37.50 |
6 | P22 | 9 | 5 | 62.50 |
7 | P25 | 11 | 3 | 27.27 |
8 | P26 | 15 | 13 | 86.67 |
9 | P807 | 15 | 13 | 86.67 |
10 | P864 | 12 | 7 | 58.33 |
总计Total | 116 | 75 | 64.66 | |
平均Average | 11.6 | 7.5 | 64.66 |
Tab.3 Primers polymorphism montained by ISSR analysis
序号 No. | 引物编号 Primer No. | 扩增条带数 No.of bands(n) | 多态性 条带数 No.of polymorphic bands (k) | 多态位 点比率 Fraction of polymorphic ( p /100% ) |
---|---|---|---|---|
1 | P2 | 7 | 3 | 42.86 |
2 | P4 | 14 | 11 | 78.57 |
3 | P5 | 13 | 9 | 69.23 |
4 | P20 | 11 | 8 | 72.73 |
5 | P21 | 9 | 3 | 37.50 |
6 | P22 | 9 | 5 | 62.50 |
7 | P25 | 11 | 3 | 27.27 |
8 | P26 | 15 | 13 | 86.67 |
9 | P807 | 15 | 13 | 86.67 |
10 | P864 | 12 | 7 | 58.33 |
总计Total | 116 | 75 | 64.66 | |
平均Average | 11.6 | 7.5 | 64.66 |
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