Construction of genetic linkage map and QTL localization of fruit major traits based on interspecific hybrid F2 population of pepper

doi:10.6048/j.issn.1001-4330.2023.05.014

Xinjiang Agricultural Sciences ›› 2023, Vol. 60 ›› Issue (5): 1150-1161.DOI: 10.6048/j.issn.1001-4330.2023.05.014

• Horticultural Special Local Products·Storage and Preservation Processing·Plant Protection • Previous Articles Next Articles

Construction of genetic linkage map and QTL localization of fruit major traits based on interspecific hybrid F₂ population of pepper

YUAN Lei¹^,²(), TANG Yaping², ZHANG Guoru², JI Xuehua¹(), YANG Shengbao²()

1. College of Agriculture, Shihezi University/Xinjiang Production and Construction Corps Key Laboratory of Special Fruits and Vegetables Cultivation Physiology and Germplasm Resources Utilization,Shihezi Xinjiang 832003,China
2. Institute of Horticulture, Xinjiang Academy of Agricultural Sciences, Urumqi 830091,China

Received:2022-09-03 Online:2023-05-20 Published:2023-05-22
Correspondence author: JI Xuehua (1977-), female, from Shihezi, Xinjiang, professor, research direction: vegetable cultivation and breeding research.(E-mail)lilysnowjxh@163.com;YANG Shengbao (1980-), male, from Miquan, Xinjiang, researcher, research direction is vegetable genetics and breeding, (E-mail)ysb.jack@163.com
Supported by:
National Natural Science Foundation of China(32060676);National Natural Science Foundation of China(31860548);National Natural Science Foundation of China(32060680);China Agriculture Research System(CARS-24-G-29)

基于辣椒种间杂交F₂群体的遗传连锁图谱构建及主要果实性状QTL定位

袁雷¹^,²(), 唐亚萍², 张国儒², 吉雪花¹(), 杨生保²()

1.石河子大学农学院/特色果蔬栽培生理与种质资源利用兵团重点实验室,新疆石河子 832003
2.新疆农业科学院园艺作物研究所, 乌鲁木齐 830091

通讯作者: 吉雪花(1977 -),女,新疆石河子人,教授,研究方向为蔬菜栽培与育种,(E-mail)lilysnowjxh@163.com;杨生保(1980 -),男,新疆米泉人,研究员,研究方向为蔬菜遗传育种,(E-mail ) ysb.jack@163.com
作者简介:袁雷(1998 -),男,四川平昌人,硕士研究生,研究方向为蔬菜育种,(E-mail ) 2273747884@qq.com
基金资助:
国家自然科学基金项目(32060676);国家自然科学基金项目(31860548);国家自然科学基金项目(32060680);国家现代农业产业技术体系建设专项资金项目(CARS-24-G-29)

Abstract

Abstract:

【Objective】 The mining of key genes for the main traits of pepper fruit is of great significance for promoting the innovation of pepper germplasm and the selection and breeding of new varieties.【Methods】 In this study, the F₂generation population obtained by the hybridization of ZL-280 (C.chinense) and GB-35(C.annuum) was used as the material to construct the interspecific genetic map of pepper, and the main traits of the fruit were analyzed by composite interval mapping (CIM).【Results】 A high-density interspecific genetic map of pepper containing 12 linkage groups with a total distance of 1,233.87 cM and an average genetic distance between markers of 0.59 cM was constructed.Through joint data analysis, a total of three QTL sites associated with color valence were detected, namely cv.2.1, cv.4.1 and cv.5.1 located on chromosomes 2nd, 4th and 5th, with contribution rates of 8.58%, 10.66% and 12.44%, respectively.Two QTL sites associated with capsaicin were detected, c.2.1 and c.5.1 on chromosomes 2 and 5, with contribution rates of 7.14% and 11.40%, respectively.Three QTL sites associated with capsaicin were detected, cap.1.1, cap.2.1 and cap.8.1 located on chromosomes 1, 2 and 8, respectively, with a contribution rate of 13.00%, 18.95% and 13.29%.There were three QTL sites associated with the longitudinal diameter of the fruit, namely fl.2.1, fl.2.2 and fl.4.1 on chromosomes 2 and 4, with a contribution rate between 6.18%, 4.71% and 4.01%.There was 1 QTL site associated with the transverse diameter of the fruit, located on fd.6.1 on chromosome 6, with a contribution rate of 10.07%; Two QTL sites associated with placenta size were detected, respectively, ps.2.1 and ps.7.1 located on chromosomes 2 and 7, with contribution rates of 4.55% and 5.35%; One QTL site associated with thick pulp was pt.11.1 located on chromosome 11, with a contribution rate of 1.64%; One QTL site associated with single fruit reassociation was detected, sfw.7.1 located on chromosome 7, with a contribution rate of 4.33%.【Conclusion】 In this study, an interspecific genetic map of high-density pepper was constructed, and 16 QTLs sites associated with the main traits of pepper fruit were detected, and the contribution rate ranged from 1.64%~18.95%.

Key words: pepper; fruit traits; genetic linkage map; QTL localization

摘要：

【目的】研究辣椒果实主要性状关键基因,为挖掘辣椒果实功能基因和新品种选育提供参考。【方法】以ZL-280(C.chinense)和GB-35(C.annuum)杂交获得的F₂代群体为材料,构建辣椒种间遗传图谱,采用复合区间作图法(CIM)果实主要性状进行QTL分析。【结果】构建了一张包含12个连锁群,总图距为1 233.87 cM,标记间平均遗传距离为0.59 cM的辣椒高密度种间遗传图谱。共计检测到与色价关联的3个QTL位点,分别为位于第2、4和5条染色体上的cv.2.1、cv.4.1和cv.5.1,贡献率分别为8.58%,10.66%,12.44%;检测到与辣椒红素关联的2个QTL位点,分别为位于第2、5号染色体上的c.2.1和c.5.1,贡献率分别为7.14%和11.40%;检测到与辣椒素关联的3个QTL位点,分别位于第1、2和8号染色体上的cap.1.1、cap.2.1、和cap.8.1,贡献率为13.00%,、18.95%、13.29%;与果实纵径关联的QTL位点有3个,分别为位于第2、4号染色体上的fl.2.1、fl.2.2和fl.4.1,贡献率为6.18%、4.71%和4.01%;与果实横径关联的QTL位点1个,位于第6号染色体上的fd.6.1,贡献率为10.07%;检测到与胎座大小关联的QTL位点2个,分别为位于第2号和第7号染色体上的ps.2.1和ps.7.1,贡献率为4.55%和5.35%;1个与果肉厚关联的QTL位点,为位于第11号染色体上的pt.11.1,贡献率为1.64%;检测到与单果重关联的QTL位点1个,位于第7号染色体上sfw.7.1,贡献率为4.33%。【结论】构建了一张高密度辣椒种间遗传图谱,检测到16个与辣椒果实主要性状关联的QTLs位点,贡献率范围为1.64%~18.95%。

关键词: 辣椒, 果实性状, 遗传连锁图谱, QTL定位

CLC Number:

S641.3

YUAN Lei, TANG Yaping, ZHANG Guoru, JI Xuehua, YANG Shengbao. Construction of genetic linkage map and QTL localization of fruit major traits based on interspecific hybrid F₂ population of pepper[J]. Xinjiang Agricultural Sciences, 2023, 60(5): 1150-1161.

袁雷, 唐亚萍, 张国儒, 吉雪花, 杨生保. 基于辣椒种间杂交F₂群体的遗传连锁图谱构建及主要果实性状QTL定位[J]. 新疆农业科学, 2023, 60(5): 1150-1161.

Figures/Tables 10

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材料编号 Material number	种名 Species Type	色价 Color value	辣椒红素含量 Capsanthin (mg/g)	辣椒素 Capsaicin (mg/g)	果实纵径 Fruit length (cm)	果实横径 Fruit diameter (cm)	胎座大小 Placenta size (cm)	果肉厚 Pulp thickness (mm)	单果重 Single fruit weight (g)
GB-35	一年生栽培种	13.63	3.88	1.28	16.975	1.461	3.220	0.160	27.38
ZL-280	中国种辣椒	4.6	1.60	37.26	4.670	2.590	2.610	0.210	6.3

材料编号 Material number	种名 Species Type	色价 Color value	辣椒红素含量 Capsanthin (mg/g)	辣椒素 Capsaicin (mg/g)	果实纵径 Fruit length (cm)	果实横径 Fruit diameter (cm)	胎座大小 Placenta size (cm)	果肉厚 Pulp thickness (mm)	单果重 Single fruit weight (g)
GB-35	一年生栽培种	13.63	3.88	1.28	16.975	1.461	3.220	0.160	27.38
ZL-280	中国种辣椒	4.6	1.60	37.26	4.670	2.590	2.610	0.210	6.3

指标 Index	最大值 Max.	最小值 Min.	均值 Mean	标准差 SD	变异幅度 Variation range	变异系数 CV(%)
色价Color value	25.94	5.08	12.40	3.53	20.86	28.45
辣椒红素Capsanthin(mg/g)	4.71	1.49	3.20	1.21	4.28	12.35
辣椒素Capsaicin(mg/g)	18.21	0.47	5.29	3.05	17.74	57.66
果实纵径Fruit length(cm)	24.94	2.35	11.11	3.27	22.59	29.39
果实横径Fruit diameter(cm)	2.99	1.08	1.68	0.35	1.91	20.58
胎座大小Placenta size(cm)	1.68	0.15	0.60	0.19	1.53	31.53
果肉厚Pulp thickness(cm)	0.52	0.10	0.18	0.05	0.42	26.94
单果重Single fruit weight(g)	23.95	2.52	9.36	3.44	21.43	36.74

指标 Index	最大值 Max.	最小值 Min.	均值 Mean	标准差 SD	变异幅度 Variation range	变异系数 CV(%)
色价Color value	25.94	5.08	12.40	3.53	20.86	28.45
辣椒红素Capsanthin(mg/g)	4.71	1.49	3.20	1.21	4.28	12.35
辣椒素Capsaicin(mg/g)	18.21	0.47	5.29	3.05	17.74	57.66
果实纵径Fruit length(cm)	24.94	2.35	11.11	3.27	22.59	29.39
果实横径Fruit diameter(cm)	2.99	1.08	1.68	0.35	1.91	20.58
胎座大小Placenta size(cm)	1.68	0.15	0.60	0.19	1.53	31.53
果肉厚Pulp thickness(cm)	0.52	0.10	0.18	0.05	0.42	26.94
单果重Single fruit weight(g)	23.95	2.52	9.36	3.44	21.43	36.74

样本名称 Sample ID	序列数 Reads	碱基数 Base	Q30 (%)	GC (%)
GB-35	16 303 373	3 253 979 334	96.43	39.51
ZL-280	16 067 790	3 206 054 408	96.39	40.11
F₂代	6 969 044	1 392 101 632	96.10	39.08
总量 Total	1 593 436 940	318 290 799 252	96.11	39.43
对照 Control	5 483 107	1 094 703 458	95.71	45.30