Analysis on related physiological indexes of pepper with hair characteristic under chilling stress

doi:10.6048/j.issn.1001-4330.2023.06.023

Abstract

Abstract:

【Objective】 To study the cold resistance and adaptability to low temperature of trichome germplasm.So as to provide a scientific reference for the development and utilization of resources in the next step.【Methods】 In this experiment, the characteristic variety of Qinghai province downy linear pepper cultivar ‘Qingxianjiao 2’ was used as the test material. The plants were treated with low temperature at seedling stage, and the activity of superoxide dismutase (SOD), the content of soluble sugar, soluble protein content and malondialdehyde (MDA) were determined under different treatments.【Results】 The results showed that with the extension of stress time, the activity of superoxide dismutase (SOD), the trend of soluble sugar, soluble protein content were firstly increased and then decreased, while the trend of malondialdehyde (MDA) content was firstly decreased and then increased and again decreased. With the decrease of treatment temperature, the changes of different physiological indexes in each treatment group tended to be gentle. Correlation analysis showed that soluble sugar content was significantly negatively correlated with soluble protein content, and malondialdehyde content was significantly positively correlated with soluble protein content.【Conclusion】 Combined with the significant difference and correlation analysis of each index, the purpose is to evaluate the cold resistance of special germplasm resources through biochemical indicators, indicating that hairy germplasm resources have better cold resistance and adaptability to low temperature.

Key words: pepper; low temperature stress; physiological indicators; seedling stage

摘要：

【目的】研究茸毛型线辣椒种质资源的抗寒性以及对低温的适应性,为资源的开发利用提供参考依据。【方法】以青海省特色品种多茸毛型辣椒青线椒2号为材料,采用低温培养箱对幼苗期植株进行低温处理,测定不同处理下超氧化物歧化酶(SOD)活性、可溶性糖含量、可溶性蛋白含量、丙二醛(MDA)含量。【结果】随着胁迫时间的延长,超氧化物歧化酶(SOD)活性、可溶性糖含量、可溶性蛋白质含量、变化趋势均为先升高后降低,丙二醛(MDA)含量变化趋势为先降低后升高再降低;随着处理温度的降低,各处理组的不同生理指标变化相对趋势趋于平缓;可溶性糖含量与可溶性蛋白含量呈极显著负相关,丙二醛含量与可溶性蛋白含量呈显著正相关。【结论】茸毛型线辣椒种质资源具有更好的抗寒性以及对低温的适应性。

关键词: 辣椒, 低温胁迫, 生理指标, 苗期

CLC Number:

S641.3

CHEN Yan, HUANG Luyao, DENG Changrong, ZHANG Yanjun, HOU Quangang, SHAO Dengkui. Analysis on related physiological indexes of pepper with hair characteristic under chilling stress[J]. Xinjiang Agricultural Sciences, 2023, 60(6): 1492-1498.

陈艳, 黄璐瑶, 邓昌蓉, 张彦君, 侯全刚, 邵登魁. 冷害对多茸毛型线辣椒幼苗生理水平的影响[J]. 新疆农业科学, 2023, 60(6): 1492-1498.

Figures/Tables 9

References 24

[1]	常彩涛. 辣椒耐低温研究概述[J]. 辣椒杂志, 2011, 9(1):6-8, 40
	CHANG Caitao. Review of Low-Tem-perature Tolerance in Hot Pepper[J]. Journal of China Capsicum, 2011, 9(1):6-8, 40.
[2]	张国胜, 伏洋, 杨琼, 等. 青海省天然草地类型空间分布特征及气候分区[J]. 草业科学, 2009, 26(1):23-29.
	ZHANG Guosheng, FU Yang, YANG Qiong, et al. Characteristic of spatial distribution and climate zone of natural grassland types in Qinghai[J]. Pratacultual Science, 2009, 26(1):23-29.
[3]	熊先军, 刘明月. 辣椒抗寒性生理生化研究进展[J]. 辣椒杂志, 2003,(2):9-12.
	XIONG Xianjun, LIU Mingyue. Studi-es on Physiology and Bioehemistry of the Chilling resistance in Capsicum[J]. Journal of China Capsicum, 2003,(2):9-12.
[4]	N.W.西蒙兹英. 作物进化[M]. 北京: 农业出版社, 1987.
	N.W. Symonds (UK). Crop evolution[M]. Beijing: Agriculture Press, 1987.
[5]	邢光南, 赵团结, 王柬人, 等. 大豆叶茸毛着生状态的变异及其与豆卷叶螟抗性的相关性[J]. 大豆科学, 2009, 28(5):768-773.
	XING Guangnan, ZHAO Tuanjie, WANG Jianren, et al. Variation of Leaf Pubescence Sta-tus and Its Association with Resistance to Bean Pyralid(Lamprosem a indicata Fabricius) in Soybean[J]. Soybean Science, 2009, 28(5):768-773.
[6]	尚宏芹, 刘建萍. 不同茸毛性状辣椒干旱胁迫的相关生理指标分析[J]. 中国蔬菜, 2010,(14):49-53.
	SHANG Hongqin, LIU Jianping. Analysis on Related Physiological Indexes of Pepper with Different Hair Characteristics under Drought Stress[J]. China Vegetables, 2010,(14):49-53.
[7]	尚宏芹, 高昌勇, 刘建萍. 不同植物体表茸毛的研究进展[J]. 安徽农业科学, 2004,(5):1009-1012.
	SHANG Hongqin, GAO Changyong, LIU Jianping. Research progress of trichome on different plants[J]. Journal of Anhui Agricultural Sciences, 2004,(5):1009-1012.
[8]	陈建华, 项显林. 棉花茸毛性状与抗蚜的关系[J]. 中国棉花, 1981,(2):20-23.
	CHEN Jianhua, XIANG Xianlin. Relationship between Trichome traits and Resistance to Aphid in Cotton[J]. China Cotton, 1981,(2):20-23.
[9]	尚宏芹. 辣椒体表茸毛的形态结构与分布特点研究[J]. 安徽农业科学, 2009, 37(1):124-125.
	SHANG Hongqin. Study on Morphological Structure and Distribution Features of Hairs on Capsicum[J]. Journal of Anhui Agricultural Sciences, 2009, 37(1):124-125.
[10]	邵登魁, 侯全刚, 李莉, 等. 黄色线辣椒新品种‘青线椒2号’[J]. 园艺学报, 2013, 40(1):186-188.
	SHAO Dengkui, HOU Quangang, LI Li, et al. A New Yellow Linear Hot Pepper Cultivar ‘Qingxianjiao 2’[J]. Acta Horticulturae Sinica, 2013, 40(1):186-188.
[11]	赵永泉, 公月敏. 辣椒优质高效栽培技术[J]. 吉林蔬菜, 2019,(2):3.
	ZHAO Yongquan, GONG Yuemin. High quality and efficient cultivation technology of pepper[J]. Jilin Vegetables, 2019,(2):3.
[12]	王兴娥. 冷诱导基因CBF4在辣椒中的遗传转化及抗寒性分析[D]. 杨凌: 西北农林科技大学, 2009.
	WANG Xinge. Transformation of Pepper with Cold-induced CBF4 Gene and Analysis of Cold hardness related Indexes[D]. Yangling: Northwest A&F University, 2009.
[13]	李振庆. 棉花幼苗低盐驯化后对高盐胁迫的生理响应[D]. 北京: 中国农业科学院, 2014.
	LI Zhenqing. Physiological Responses of Cotton to Salt Stress after Adaptation under Low Salinity in see-ding stage[D]. Beijing: Chinese Academy of Agricultural Sciences, 2014.
[14]	张述伟, 宗营杰, 方春燕, 等. 蒽酮比色法快速测定大麦叶片中可溶性糖含量的优化[J]. 食品研究与开发, 2020, 41(7):196-200.
	ZHANG Shuwei, ZONG Yingjie, FANG Chunyan, et al. Optimization of rapid determination of soluble sugar content in barley leaves by anthrone colorimetry[J]. Food Research and Development, 2020, 41(7):196-200.
[15]	王孝平, 邢树礼. 考马斯亮蓝法测定蛋白含量的研究[J]. 天津化工, 2009, 23(3): 40-42.
	WANG Xiaoping, XING Shuli. Determination of protein quantitation using the method of coomassie brilliant blue[J]. Tianjin Chemical Industry, 2009, 23(3): 40-42.
[16]	张宪政, 谭桂茹. 植物生理学实验技术[M]. 沈阳: 辽宁科学技术出版社, 1989.
	ZHANG Xianzheng, TAN Guiru. Exper-imental techniques of plant physiology[M]. Shenyang: Liaoning Science and Technology Press, 1989.
[17]	Manzer H,. Siddiqui, Mutahhar Y. Al-Khaishany, et al. Morphological and physiological characterization of different genotypes of faba bean under heat stress[J]. Saudi Journal of Biological Sciences, 2015, 22(5).
[18]	魏婧, 徐畅, 李可欣, 等. 超氧化物歧化酶的研究进展与植物抗逆性[J]. 植物生理学报, 2020, 56(12):2571-2584.
	WEI Jing, XU Chang, LI Kexin, et al. Research progress of superoxide dismutase and plant stress resistance[J]. Chin J Plant Physiology, 2020, 56(12):2571-2584.
[19]	刘文英, 于克明, 滕飞, 等. 耐低温植物黄花蒿(Avena nuda L.)低温诱导的生理响应[J]. 科学世界杂志, 2013:658793.
	Wenying L, Kenming Y, Tengfei H, et al. The Low Temperature Induced Physiological Responses of Avena nuda L. a Cold-Tolerant Plant Species[J]. Scientific World Journal, 2013:658793.
[20]	赵一航, 孟令东, 张晓萌, 等. 4个紫花苜蓿品种对低温胁迫的生理响应及抗寒性评价[J]. 草业科学, 2021, 38(4):683-692.
	ZHAO Yihang, MENG Lingdong, ZHANG Xiaoming, et al. Evaluation of physiological response and cold resistance of four alfalfa cultivars to low temperature stress[J]. Pratacultural Science, 2021, 38(4):683-692.
[21]	张桂莲, 张顺堂, 肖浪涛, 等. 花期高温胁迫对水稻花药生理特性及花粉性状的影响[J]. 作物学报, 2013, 39(1):177-183.
	ZHANG Guilian, ZHANG Shuntang, XIAO Langtao, et al. Effect of High Temperature Stress on Physiological Characteristics of Anther and Pollen Traits of Rice at Flowering Stage[J]. Acta Agronomica Sinica, 2013, 39(1):177-183. DOI URL
[22]	程嘉惠, 张梅丽, 王超, 等. 低温胁迫对4个草莓品种生理指标的影响[J]. 东北农业科学, 2021, 46(1):85-88, 113.
	CHENG Jiahui, ZHANG Meili, WANG Chao, et al. Effect of Low Temperature Stress on Physiological Index of Four Strawberry Varieties[J]. Journal of Northeast Agricultural Sciences, 2021, 46(1):85-88, 113.
[23]	乌凤章, 王贺新, 国辉, 等. 木本植物低温胁迫生理及分子机制研究进展[J]. 林业科学, 2015, 51(7):116-128.
	WU Fengzhang, WANG Hexin, XU Guohui, et al. Research Progress on the Physiological and Molecular Mechanisms of Woody Plants under Low Temperature Stress[J]. Scientia Silvae Sinicae, 2015, 51(7):116-128.
[24]	李红霞, 汪妤, 张战凤, 等. 植物转录因子与作物抗逆胁迫关系的研究进展[J]. 麦类作物学报, 2013, 33(3): 613-618.
	LI Hongxia, WANG Yu, ZHANG Zhanfeng, et al. Progress in Relationship between Plant Transcription Factors and Crop Stress Tolerance[J]. Journal of Triticeae Crops, 2013, 33(3): 613-618.

处理 Treament	15 d	30 d	45 d	60 d
处理1 Treament 1	104.797^c	123.527^b	118.28^a	101.533^a
处理2 Treament 2	118.890^b	138.643^a	126.413^a	105.050^ab
处理3 Treament 3	109.757^a	128.280^ab	105.98^b	94.947^bc
处理4 Treament 4(CK)	85.517^d	91.067^c	94.703^c	90.400^c

处理 Treament	15 d	30 d	45 d	60 d
处理1 Treament 1	104.797^c	123.527^b	118.28^a	101.533^a
处理2 Treament 2	118.890^b	138.643^a	126.413^a	105.050^ab
处理3 Treament 3	109.757^a	128.280^ab	105.98^b	94.947^bc
处理4 Treament 4(CK)	85.517^d	91.067^c	94.703^c	90.400^c

处理 Treament	15 d	30 d	45 d	60 d
处理1 Treament 1	7.952^a	7.490^b	12.370^a	9.001^a
处理2 Treament 2	7.452^a	8.777^b	8.78^b	4.942^c
处理3 Treament 3	7.780^a	10.160^a	12.99^a	6.979^b
处理4 Treament 4(CK)	3.496^b	5.432^c	6.890^c	3.400^d

处理 Treament	15 d	30 d	45 d	60 d
处理1 Treament 1	7.952^a	7.490^b	12.370^a	9.001^a
处理2 Treament 2	7.452^a	8.777^b	8.78^b	4.942^c
处理3 Treament 3	7.780^a	10.160^a	12.99^a	6.979^b
处理4 Treament 4(CK)	3.496^b	5.432^c	6.890^c	3.400^d

处理 Treament	15 d	30 d	45 d	60 d
处理1 Treament 1	28.802^a	28.903^b	11.012^b	11.282^b
处理2 Treament 2	5.861^d	58.778^a	36.456^a	11.053^b
处理3 Treament 3	10.194^c	28.067^b	35.317^ab	11.434^b
处理4 Treament 4(CK)	15.042^b	64.684^a	24.272^a	16.726^a