Evaluation of saline/alkali tolerance of new transgenic High-Yield cotton at germination and seedling stages

doi:10.6048/j.issn.1001-4330.2023.09.019

Xinjiang Agricultural Sciences ›› 2023, Vol. 60 ›› Issue (9): 2239-2247.DOI: 10.6048/j.issn.1001-4330.2023.09.019

• Plant Protection·Microbes • Previous Articles Next Articles

Evaluation of saline/alkali tolerance of new transgenic High-Yield cotton at germination and seedling stages

SONG Bingmei¹^,²(), JIANG Yan², CHEN Xin²^,³, ZHANG Yu⁴, CHENG Wannan⁴, PAN Hongsheng²()

1. Xinjiang Key Laboratory of Biological Resources and Genetic Engineering/ College of Life Science and Technology, Xinjiang University, Urumqi 830046, China
2. National Plant Protection Scientific Observation and Experiment Station in Korla/Institute of Plant Protection, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China
3. Engineering Research Center of Cotton, Ministry of Education/College of Agriculture, Xinjiang Agricultural University, Urumqi 830052, China
4. State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received:2022-12-10 Online:2023-09-20 Published:2023-09-19
Correspondence author: PAN Hongsheng (1982-), male, native place: Zhaodong, Heilongjiang Province, researcher, master tutor, research direction for safety evaluation of transgenic crops, (E- mail) panhongsheng0715@163.com
Supported by:
Stable Support to Agricultural Sci-Tech Renovation(xjnkywdzc-2022004);Project Tianshan Talent Cultivation Plan of Xinjiang Autonomous Region(2022TSYCCX0086);Korla Comprehensive Experimental Station of Xinjiang Cotton Industry Technology System;Special Incubation Project of Science & Technology Renovation of Xinjiang Academy of Agricultural Sciences(xjkcpy-2020004)

新型转基因高产棉花萌发期和苗期耐盐性与耐碱性评价

宋冰梅¹^,²(), 姜岩², 陈鑫²^,³, 张宇⁴, 程宛楠⁴, 潘洪生²()

1.新疆大学生命科学与技术学院/新疆生物资源和基因工程重点实验室,乌鲁木齐 830046
2.新疆农业科学院植物保护研究所/国家植物保护库尔勒观测实验站,乌鲁木齐 830091
3.新疆农业大学农学院/棉花教育部工程研究中心,乌鲁木齐 830052
4.中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室,北京 100193

通讯作者: 潘洪生(1982-),男,黑龙江肇东人,研究员,博士,研究方向为转基因作物安全性评价,(E-mail)panhongsheng0715@163.com
作者简介:宋冰梅(1999-),女,四川自贡人,硕士研究生,研究方向为转基因作物安全性评价,(E-mail)2446620106@qq.com
基金资助:
农业科技创新稳定支持项目(xjnkywdzc-2022004);新疆维吾尔自治区天山英才培养计划(2022TSYCCX0086);新疆棉花产业技术体系库尔勒综合试验站;新疆农业科学院科技创新重点培育专项(xjkcpy-2020004)

Abstract

Abstract:

【Objective】 This study aims to explore the effects of saline/alkali stress on the growth and development of new transgenic high-yield cotton at germination stage and seedling stage, and evaluate its saline/alkali tolerance. 【Methods】 In this project, transgenic Bn-csRRM2 high-yield cotton HN9311 and CCRI-12 (parent control) were used as study materials, the values and differences of growth and development related indexes were calculated and analyzed under saline stress (150 mmol/L NaCl) and alkali stress (70 mmol/L NaHCO₃). 【Results】 Except the number of true leaves and root/shoot, the other direct indexes of HN9311 at germination stage and seedling stage significantly decreased under saline stress and alkali stress when compared with those of CCRI-12; Except for relative hypocotyl length and relative germination index under alkali stress at germination stage, and relative root/shoot under alkali stress at seedling stage, the other relative indexes of HN9311 at germination and seedling stages were significantly lower than or equal to those of CCRI-12; The relative saline/alkali damage rates of HN9311 at germination stage were 27.00% and 21.00%, and those of CCRI-12 were 22.75% and 23.00%. The relative saline damage rate of HN9311was significantly higher than that of CCRI-12. 【Conclusion】 Saline/alkali stress has a significant inhibitory effect on the growth and development of HN9311 at germination stage and seedling stage. HN9311 is more sensitive to alkali stress at seedling stage when compared with saline stress, saline tolerance at germination stage and saline/alkali tolerance at seedling stage were lower than those of CCRI-12.

Key words: HN9311; germination stage; seedling stage; growth and development; saline/alkali tolerance evaluation

摘要：

【目的】研究盐/碱胁迫对新型转基因高产棉花萌发期和苗期生长发育的影响并对其耐盐性与耐碱性进行评价。【方法】以转Bn-csRRM2基因高产棉花HN9311和亲本对照中棉所12号(CCRI-12)为研究对象,在盐胁迫(150 mmol/L NaCl)和碱胁迫(70 mmol/L NaHCO₃)下,统计和分析生长发育相关指标及差异。【结果】与对照相比,除真叶数和根冠比以外,在盐胁迫和碱胁迫下HN9311萌发期和苗期各项直接指标均显著下降;除萌发期碱胁迫下相对下胚轴长和相对发芽指数以及苗期碱胁迫下相对根冠比以外,HN9311萌发期和苗期各项相对指标均显著低于或等于中棉所12号;HN9311萌发期相对盐/碱害率分别为27.00%、21.00%,中棉所12号分别为22.75%、23.00%,HN9311相对盐害率显著大于中棉所12号。【结论】盐/碱胁迫对HN9311萌发期和苗期生长发育均具有显著的抑制作用,与盐胁迫相比其苗期对碱胁迫更为敏感,萌发期耐盐胁迫能力和苗期耐盐/碱胁迫能力均低于中棉所12号。

关键词: HN9311, 萌发期, 苗期, 生长发育, 耐盐/碱性评价

CLC Number:

S512

SONG Bingmei, JIANG Yan, CHEN Xin, ZHANG Yu, CHENG Wannan, PAN Hongsheng. Evaluation of saline/alkali tolerance of new transgenic High-Yield cotton at germination and seedling stages[J]. Xinjiang Agricultural Sciences, 2023, 60(9): 2239-2247.

宋冰梅, 姜岩, 陈鑫, 张宇, 程宛楠, 潘洪生. 新型转基因高产棉花萌发期和苗期耐盐性与耐碱性评价[J]. 新疆农业科学, 2023, 60(9): 2239-2247.

Figures/Tables 5

Tab.1 Effects of saline/alkali stress on the direct index of new transgenic high-yield cotton HN9311 at germination stage

直接指标 Direct index	盐胁迫 Saline stress		碱胁迫 Alkali stress
直接指标 Direct index	0 mmol/L NaCl (CK)	150 mmol/L NaCl	0 mmol/L NaHCO₃ (CK)	70 mmol/L NaHCO₃
鲜重量Fresh weight(g)	0.36±0.01^a	0.26±0.01^b	0.36±0.01^a	0.26±0.01^b
下胚轴长Hypocotyl length(cm)	1.72±0.05^a	1.41±0.14^b	1.70±0.03^a	1.37±0.02^b
根长Root length(cm)	4.43±0.05^a	2.22±0.08^b	4.31±0.04^a	0.00±0.00^c
发芽势Germination potential(%)	66.25±0.48^a	27.50±0.29^c	67.50±0.29^a	43.00±0.91^b
发芽率Germination rate(%)	98.25±0.48^a	72.00±0.82^c	98.25±0.48^a	78.00±0.00^b
发芽指数Germination index	95.42±0.19^a	56.40±0.48^c	95.85±0.13^a	71.04±0.33^b
活力指数Vigor index	34.26±0.59^a	14.92±0.68^c	33.80±0.62^a	18.03±0.40^b

Tab.2 Effects of saline/alkali stress on the direct index of new transgenic high-yield cotton HN9311 at seedling stage

直接指标 Direct index	盐胁迫 Saline stress		碱胁迫 Alkali stress
直接指标 Direct index	0 mmol/L NaCl (CK)	150 mmol/L NaCl	0 mmol/L NaHCO₃ (CK)	70 mmol/L NaHCO₃
真叶数Number of true leaves	3.75±0.25^ab	3.25±0.25^b	4.00±0.00^a	2.25±0.08^c
株高Plant height(cm)	15.00±0.41^a	14.00±0.00^b	15.80±0.15^a	9.93±0.38^c
根长Root length(cm)	21.63±0.38^a	14.00±0.41^b	21.70±0.10^a	9.83±0.94^c
根鲜重Root fresh weight(g)	2.57±0.02^a	1.93±0.05^b	2.55±0.05^a	1.56±0.05^c
地上部鲜重Shoot fresh weight(g)	5.28±0.06^a	3.33±0.06^b	5.23±0.03^a	2.10±0.10^c
鲜重量Fresh weight(g)	7.85±0.04^a	5.25±0.10^b	7.78±0.09^a	3.66±0.15^c
根冠比Root/shoot	0.49±0.01^c	0.58±0.01^b	0.49±0.01^c	0.74±0.01^a

Tab.3 Effects of saline/alkali stress on the relative index of new transgenic high-yield cotton HN9311 at germination stage

相对指标 Relative index	盐胁迫 Saline stress		碱胁迫 Alkali stress
相对指标 Relative index	中棉所12号 CCRI-12	HN9311	中棉所12号 CCRI-12	HN9311
相对鲜重量Relative fresh weight	0.86±0.02^*	0.74±0.03	0.95±0.0 ${1^{}}^{}$	0.72±0.02
相对下胚轴长Relative hypocotyl length	0.98±0.02	0.82±0.08	0.73±0.01	0.81±0.0 ${1^{}}^{}$
相对根长Relative root length	0.56±0.03	0.50±0.02	0.28±0.00	0.28±0.01
相对发芽势Relative germination potential	0.56±0.0 ${1^{}}^{}$	0.42±0.00	0.65±0.01	0.64±0.01
相对发芽率Relative germination rate	0.77±0.0 ${1^{}}^{}$	0.73±0.01	0.78±0.00	0.79±0.00
相对发芽指数Relative germination index	0.70±0.0 ${1^{}}^{}$	0.59±0.00	0.72±0.00	0.74±0.00^*
相对活力指数 Relative vigor index	0.60±0.0 ${1^{}}^{}$	0.43±0.02	0.68±0.01	0.53±0.01

Tab.3 Effects of saline/alkali stress on the relative index of new transgenic high-yield cotton HN9311 at germination stage

相对指标 Relative index	盐胁迫 Saline stress		碱胁迫 Alkali stress
相对指标 Relative index	中棉所12号 CCRI-12	HN9311	中棉所12号 CCRI-12	HN9311
相对鲜重量Relative fresh weight	0.86±0.02^*	0.74±0.03	0.95±0.0 ${1^{}}^{}$	0.72±0.02
相对下胚轴长Relative hypocotyl length	0.98±0.02	0.82±0.08	0.73±0.01	0.81±0.0 ${1^{}}^{}$
相对根长Relative root length	0.56±0.03	0.50±0.02	0.28±0.00	0.28±0.01
相对发芽势Relative germination potential	0.56±0.0 ${1^{}}^{}$	0.42±0.00	0.65±0.01	0.64±0.01
相对发芽率Relative germination rate	0.77±0.0 ${1^{}}^{}$	0.73±0.01	0.78±0.00	0.79±0.00
相对发芽指数Relative germination index	0.70±0.0 ${1^{}}^{}$	0.59±0.00	0.72±0.00	0.74±0.00^*
相对活力指数 Relative vigor index	0.60±0.0 ${1^{}}^{}$	0.43±0.02	0.68±0.01	0.53±0.01

Tab.4 Effects of saline/alkali stress on the relative index of new transgenic high-yield cotton HN9311 at seedling stage

相对指标 Relative index	盐胁迫 Saline stress		碱胁迫 Alkali stress
相对指标 Relative index	中棉所12号 CCRI-12	HN9311	中棉所12号 CCRI-12	HN9311
相对真叶数Relative true leaf number	0.89±0.00	0.87±0.07	0.56±0.02	0.60±0.02
相对株高Relative plant height	0.95±0.03	0.93±0.00	0.77±0.01^*	0.66±0.03
相对根长Relative root length	0.60±0.01	0.65±0.02	0.59±0.03	0.46±0.04
相对根鲜重Relative root fresh weight	0.90±0.0 ${0^{}}^{}$	0.75±0.02	0.86±0.0 ${0^{}}^{}$	0.61±0.02
相对地上部鲜重Relative shoot fresh weight	0.87±0.07^*	0.63±0.01	0.75±0.0 ${1^{}}^{}$	0.40±0.02
相对鲜重量Relative fresh weight	0.88±0.0 ${4^{}}^{}$	0.67±0.01	0.79±0.0 ${1^{}}^{}$	0.47±0.02
相对根冠比Relative root/shoot	1.06±0.09	1.19±0.03	1.14±0.01	1.53±0.0 ${3^{}}^{}$

Tab.4 Effects of saline/alkali stress on the relative index of new transgenic high-yield cotton HN9311 at seedling stage

相对指标 Relative index	盐胁迫 Saline stress		碱胁迫 Alkali stress
相对指标 Relative index	中棉所12号 CCRI-12	HN9311	中棉所12号 CCRI-12	HN9311
相对真叶数Relative true leaf number	0.89±0.00	0.87±0.07	0.56±0.02	0.60±0.02
相对株高Relative plant height	0.95±0.03	0.93±0.00	0.77±0.01^*	0.66±0.03
相对根长Relative root length	0.60±0.01	0.65±0.02	0.59±0.03	0.46±0.04
相对根鲜重Relative root fresh weight	0.90±0.0 ${0^{}}^{}$	0.75±0.02	0.86±0.0 ${0^{}}^{}$	0.61±0.02
相对地上部鲜重Relative shoot fresh weight	0.87±0.07^*	0.63±0.01	0.75±0.0 ${1^{}}^{}$	0.40±0.02
相对鲜重量Relative fresh weight	0.88±0.0 ${4^{}}^{}$	0.67±0.01	0.79±0.0 ${1^{}}^{}$	0.47±0.02
相对根冠比Relative root/shoot	1.06±0.09	1.19±0.03	1.14±0.01	1.53±0.0 ${3^{}}^{}$

Fig.1 Effects of saline/alkali stress on relative saline/alkali damage rate of new transgenic high-yield cotton HN9311 at germination stage Note:* and ns respectively represent significant difference (P<0.05) or not represent significant difference (P>0.05) for the relative saline damage rate between HN9311 and CCRI-12 (independent sample T test)

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Evaluation of saline/alkali tolerance of new transgenic High-Yield cotton at germination and seedling stages

新型转基因高产棉花萌发期和苗期耐盐性与耐碱性评价

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