Xinjiang Agricultural Sciences ›› 2023, Vol. 60 ›› Issue (12): 3057-3064.DOI: 10.6048/j.issn.1001-4330.2023.12.022
• Animal Husbandry Veterinarian·Microbes·Agricultural Equipment Engineering and Mechanization • Previous Articles Next Articles
LIU Shengxue1(), LI Siqi2, WANG Xiaodong2, YANG Desong2()
Received:
2023-03-21
Online:
2023-12-20
Published:
2024-01-03
Correspondence author:
YANG Desong(1977-),male, Doctor, Professor, Research direction: Pesticide toxicology, (E-mail)Supported by:
通讯作者:
杨德松(1977-),男,安徽滁州人,教授,博士,研究方向为农药环境毒理学, (E-mail)yds_agr@shzu.edu.cn
作者简介:
刘升学(1974-),男,甘肃通渭人,副教授,博士,研究方向植物病理学,(E-mail)liushengxue-1@163.com
基金资助:
CLC Number:
LIU Shengxue, LI Siqi, WANG Xiaodong, YANG Desong. Diversity analysis of dsRNA in Alternaria solani[J]. Xinjiang Agricultural Sciences, 2023, 60(12): 3057-3064.
刘升学, 李思琪, 王晓东, 杨德松. 番茄早疫病菌dsRNA多样性分析[J]. 新疆农业科学, 2023, 60(12): 3057-3064.
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URL: http://www.xjnykx.com/EN/10.6048/j.issn.1001-4330.2023.12.022
Fig.1 Detection of dsRNA in Alternaria solani isolate Note:M, DNA Maker; A、AS、T、G、H, strain(a.the samples collected in 2015,b.collected in 2016,c and d.collected different season in 2022)
序号 No. | 菌株 Strain | Contigs | 已报到病毒Known virus | 同源性 Per. Ident (%) | 核酸 类型 Nucleotide type | 分类 Taxonomy | |||
---|---|---|---|---|---|---|---|---|---|
组分 Component | 大小 Size(nt) | 登录号 GenBank | 名称/分离物/株系 Name/Isolate/Strain | ||||||
1 | AS24 | dsRNA-L | 2 537 | U41345 | HvV 190S | 63 | dsRNA | Totiviridae | |
MN935504 | BmV1c/CY27 | 62 | |||||||
MN935505 | BmV1d/CY27 | 62 | |||||||
MN935503 | BmV1b/CY27 | 62 | |||||||
MH396496 | BmV1 TMZS01 | 62 | |||||||
dsRNA-S | 1 483 | MT648467 | AtPV1 | 54 | dsRNA | Partitiviridae | |||
MN556985 | PVLaPV10 DMG-B_DN51686 | 53 | |||||||
MN605502 | ENaPV8 PMS8_DN6389 | 51 | |||||||
2 | G3 | 1 | 1 189 | NC_040793 | AaVV1 | 74 | dsRNA | Totiviridae | |
KT598230 | CmRV CmRV-IL | 59 | |||||||
2 | 694 | HE579658 | dsRNA | Totiviridae | |||||
HE579659 | Uncultured totivirus | 90 | |||||||
HE579660 | |||||||||
3 | 889 | HE579658 | |||||||
HE579659 | Uncultured totivirus | 100 | |||||||
HE579660 | |||||||||
3 | S8 | 1 | 3 411 | NC_040793 | AaVV1 | 92 | dsRNA | Totiviridae | |
KT598230 | CmRV CmRV-IL | 92 | |||||||
MK758089 | BmVV2 CY05 | 81 | |||||||
2 | 559 | KJ605397 | UvNV-1 GX-10 | 23 | dsRNA | Partitiviridae | |||
4 | T4 | 4 | 371 | MW328751 | TpMV1 | 99 | +ssRNA | Tymoviridae | |
5 | H74 | 1 | 3 262 | KJ817371 | AlRV1 HN28 | 99 | dRNA | 未分类 | |
6 | H63 | 1 | 3 684 | MK584808 | AaCV1 | dsRNA | Chrysoviridae | ||
MN563140 | CREA-VE-32SY2 RNA1 | 98 | |||||||
NC_040738 | |||||||||
MK672910 | AaCV1 QY-2 RNA1 | 86 | |||||||
2 | 2 532 | MK584809 | AaCV1 CREA-VE-32SY2 RNA2 | 83 | |||||
NC_040739 | AaCV1 RNA2 | 25 | |||||||
4 | 1 105 | MK584810 | AaCV1 | 97 | |||||
NC_040736 | CREA-VE-32SY2 RNA3 | ||||||||
5 | 1 418 | MK584811 | AaCV1 CREA-VE-32SY2 RNA4 | 64 | |||||
NC_040740 | AaCV1 RNA4 | 99 | |||||||
3 | 825 | MN605440 | ENaNV27 PMS2_317 | 93 | +ssRNA | Narnaviridae |
Tab.1 BLAST results for sequence determined
序号 No. | 菌株 Strain | Contigs | 已报到病毒Known virus | 同源性 Per. Ident (%) | 核酸 类型 Nucleotide type | 分类 Taxonomy | |||
---|---|---|---|---|---|---|---|---|---|
组分 Component | 大小 Size(nt) | 登录号 GenBank | 名称/分离物/株系 Name/Isolate/Strain | ||||||
1 | AS24 | dsRNA-L | 2 537 | U41345 | HvV 190S | 63 | dsRNA | Totiviridae | |
MN935504 | BmV1c/CY27 | 62 | |||||||
MN935505 | BmV1d/CY27 | 62 | |||||||
MN935503 | BmV1b/CY27 | 62 | |||||||
MH396496 | BmV1 TMZS01 | 62 | |||||||
dsRNA-S | 1 483 | MT648467 | AtPV1 | 54 | dsRNA | Partitiviridae | |||
MN556985 | PVLaPV10 DMG-B_DN51686 | 53 | |||||||
MN605502 | ENaPV8 PMS8_DN6389 | 51 | |||||||
2 | G3 | 1 | 1 189 | NC_040793 | AaVV1 | 74 | dsRNA | Totiviridae | |
KT598230 | CmRV CmRV-IL | 59 | |||||||
2 | 694 | HE579658 | dsRNA | Totiviridae | |||||
HE579659 | Uncultured totivirus | 90 | |||||||
HE579660 | |||||||||
3 | 889 | HE579658 | |||||||
HE579659 | Uncultured totivirus | 100 | |||||||
HE579660 | |||||||||
3 | S8 | 1 | 3 411 | NC_040793 | AaVV1 | 92 | dsRNA | Totiviridae | |
KT598230 | CmRV CmRV-IL | 92 | |||||||
MK758089 | BmVV2 CY05 | 81 | |||||||
2 | 559 | KJ605397 | UvNV-1 GX-10 | 23 | dsRNA | Partitiviridae | |||
4 | T4 | 4 | 371 | MW328751 | TpMV1 | 99 | +ssRNA | Tymoviridae | |
5 | H74 | 1 | 3 262 | KJ817371 | AlRV1 HN28 | 99 | dRNA | 未分类 | |
6 | H63 | 1 | 3 684 | MK584808 | AaCV1 | dsRNA | Chrysoviridae | ||
MN563140 | CREA-VE-32SY2 RNA1 | 98 | |||||||
NC_040738 | |||||||||
MK672910 | AaCV1 QY-2 RNA1 | 86 | |||||||
2 | 2 532 | MK584809 | AaCV1 CREA-VE-32SY2 RNA2 | 83 | |||||
NC_040739 | AaCV1 RNA2 | 25 | |||||||
4 | 1 105 | MK584810 | AaCV1 | 97 | |||||
NC_040736 | CREA-VE-32SY2 RNA3 | ||||||||
5 | 1 418 | MK584811 | AaCV1 CREA-VE-32SY2 RNA4 | 64 | |||||
NC_040740 | AaCV1 RNA4 | 99 | |||||||
3 | 825 | MN605440 | ENaNV27 PMS2_317 | 93 | +ssRNA | Narnaviridae |
[1] | 赵思峰, 廖依学, 王昌, 等. 新疆加工番茄病虫草害发生概况及综合防治技术[J]. 新疆农业科学, 2004, 41(5):274-276. |
ZHAO Sifeng, LIAO Yixue, WANG Chang, et al. General Occurrent Situation of Processing Tomato Diseases Pests and Weeds and Their Integrated Management Techniques in Xinjiang[J]. Xinjiang Agricultural Sciences, 2004, 41(5):274-276. | |
[2] |
Nuss D L. Biological control of chestnut blight: An example of virus-mediated attenuation of fungal pathogenesis[J]. Microbiological Reviews, 1992, 56(4):561-576.
DOI PMID |
[3] |
Hollings M. Viruses associated with a die-back disease of cultivated mushroom[J]. Nature, 1962, 196(4858):962-965.
DOI |
[4] | 胡开辉, 陈体强. 植物病原真菌及食用菌病毒研究及进展[J]. 福建农业学报, 1999, 14 (S1):190-200. |
HU Kaihui, CHEN Tiqiang. Study and progress in the mycovirus of plant pathogenic fungi and edible fungi[J]. Fujian Journal of Agricultural Sciences, 1999, 14 (S1):190-200. | |
[5] | 覃玥. 真菌病毒的研究进展[J]. 广西农业科学, 2005, 36(3):280-283. |
QIN Yue. Recent achievements of mycovirus research[J]. Guangxi Agricultural Sciences, 2005, 36(3):280-283. | |
[6] | Ghabrial S A and Suzuki N. Viruses of plant pathogenic fungi[J]. Annu.Rev. Phytopathol, 2009, 47:353-384. |
[7] |
Dodds J A. Association of type 1 viral-like dsRNA with clubshaped particles in hypovirulent strains of Endothiaparasitica[J]. Virology, 1980, 107(1):1-12.
PMID |
[8] |
Anagnostakis S L. Biological control of chestnut blight.[J]. Science, 1982, 215(4532):466-471.
PMID |
[9] |
Nuss D L. Biological control of chestnut blight: an example of virus mediated attenuation of fungal pathogenesis[J]. Microbiological Reviews, 1992, 56(4):561.
DOI PMID |
[10] |
Nuss D L. Hypovirulence: mycoviruses at the fungal -plant interface[J]. Nature Reviews Microbiology, 2005, 3(8):632-642.
DOI |
[11] |
Yu X, Hillman B I. A geminivirus-related DNA mycovirus that confers hypovirulence to a plant pathogenic fungus[J]. Proceedings of the National Academy of Sciences, 2010, 107(18):8387-8392.
DOI URL |
[12] |
Wu M, Zhang L, Li G, et al. Genome characterization of a debilitation-associated mitovirus infecting the phytopathogenic fungus Botrytis cinerea[J]. Virology, 2010, 406(1):117-26.
DOI PMID |
[13] |
Li P, Zhang H, Chen X, et al. Molecular characterization of a novel hypovirus from the plant pathogenic fungus Fusarium graminearum[J]. Virology, 2015, 481(Print):151-160.
DOI URL |
[14] |
曹钰晗, 李紫腾, 张静怡, 等. 我国苹果斑点落叶病菌携带dsRNA分析及一种dsRNA病毒的鉴定[J]. 中国农业科学, 2021, 54(22):4787-4799.
DOI |
CAO Yuhan, LI Ziteng, ZHANG Jingyi, et al. Analysis of dsRNA Carried by Alternaria alternata f. sp. mali in China and Identification of a dsRNA Virus[J]. Scientia Agricultura Sinica, 2021, 54(22):4787-4799. | |
[15] |
Shepherd H S. Virus like particles in tentoxin-producing strains of Alternaria alternata[J]. Journal of Virology, 1988, 62(10):3888-3891.
PMID |
[16] |
Zabalgogeazcoa I, Petrunak D, Christ B J, et al. Unencapsidated double-stranded RNA associated with membrane vesicles in isolates of Alternaria solani[J]. Mycological Research, 1997, 101(5):604-608.
DOI URL |
[17] |
Hayashi N, Tsuge T, Kobayashi H, et al. The presence of double-stranded RNAs in Alternaria alternata Japanese pear pathotype and their participation in AK-toxin productivity[J]. Japanese Journal of Phytopathology, 1988, 54(2):250-252.
DOI URL |
[18] |
Aoki N, Moriyama H, Kodama M, et al. A novel mycovirus associated with four double-stranded RNAs affects host fungal growth in Alternaria alternata[J]. Virus Research, 2009, 140(1-2):179-187.
DOI URL |
[19] |
Fuke K, Takeshita K, Aoki N, et al. The presence of double-stranded RNAs in Alternaria alternate Japanese pear pathotype is associated with morphological changes[J]. Journal of General Plant Pathology, 2011, 77:248-252.
DOI URL |
[20] |
Lin Y H, Zhang H L, Zhao C J, et al. The complete genome sequence of a novel mycovirus from Alternaria longipes strain HN28[J]. Archives of Virology, 2015, 160(2):577-580.
DOI URL |
[21] |
Komatsu K, Katayama Y, Omatsu T, et al. Genome sequence of a novel victorivirus identified in the phytopathogenic fungus Alternaria arborescens[J]. Archives of Virology, 2016, 161(6):1701-1704.
DOI PMID |
[22] |
Zhong J, Shang H H, Zhu C X, et al. Characterization of a novel single-stranded RNA virus, closely related to fusariviruses, infecting the plant pathogenic fungusAlternaria brassicicola[J]. Virus Research, 2016, 217:1-7.
DOI PMID |
[23] | 向均. 我国梨黑斑病病原菌种类及一种 dsRNA 病毒的鉴定[D]. 武汉: 华中农业大学, 2017. |
XIANG Jun. Identification of the pathogenic fungi of pear black spot disease from China and a dsRNA virus in them[D]. Wuhan: Huazhong Agricultural University, 2017. | |
[24] |
Okada R, Ichinose S, Takeshita K, et al. Molecular characterization of a novel mycovirus in Alternaria alternata manifesting two-sided effects: Down-regulation of host growth and up-regulation of host plant pathogenicity[J]. Virology, 2018, 519:23-32.
DOI PMID |
[25] | 王双超. 禾谷镰刀菌低毒病毒FgHV1的序列分析及生物学功能研究[D]. 北京: 中国农业科学院, 2013. |
WANG Shuangchao. Sequence Analysis and Biological Characteristics of Hypovirus FgHV1 from Fusarium graminearum[D]. Beijing: Chinese Academy of Agricultural Sciences, 2013. | |
[26] |
Chiba S, Salaipeth L, Lin Y H, et al. A novel bipartite double-stranded RNA mycovirus from the white root rot fungus Rosellinia necatrix: Molecular and biological characterization, taxonomic considerations, and potential for biological control[J]. Journal of Virology, 2009, 83(24):12801-12812.
DOI PMID |
[27] | Xiao Y, Li B, Fu Y, et al. Extracellular transmission of a DNA mycovirus and its use as a natural fungicide[C]. Proceedings of the National Academy of Sciences of the United States of America, 2013, 110(4):1452-1457. |
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