Screening and Evaluation of Wheat Germination and Seedling Growth Activity by 16 Plant Methanol Extracts

doi:10.6048/j.issn.1001-4330.2023.01.005

Xinjiang Agricultural Sciences ›› 2023, Vol. 60 ›› Issue (1): 32-42.DOI: 10.6048/j.issn.1001-4330.2023.01.005

• Crop Genetics and Breeding · Cultivation Physiology · Germplasm Resources · Physiology and Biochemistry • Previous Articles Next Articles

Screening and Evaluation of Wheat Germination and Seedling Growth Activity by 16 Plant Methanol Extracts

ZHENG Ruiming¹^,²(), WANG Li²^,³, ZHANG Jungao¹^,³^,⁴, YAN Rong², LI Jin¹^,³, LIANG Jing¹^,³, LEI Bin¹^,³(), ZHOU Xiaoyun¹^,³()

1. Key Laboratory of Oasis-Desert Crop Physiology Ecology and Cultivation of Ministry of Agricultural and Rural Affairs / Research Institute of Nuclear Technology and Biotechnology,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China
2. College of Agronomy, Xinjiang Agricultural University, Urumqi 830052, China
3. Xinjiang Engineering Research Center for Crop Chemical Regulation, Urumqi 830091, China
4. Xinjiang Oasis Xingyuan Agricultural Science and Technology Co., Ltd., Urumqi 830091, China

Received:2022-05-18 Online:2023-01-20 Published:2023-03-07
Correspondence author: LEI Bin (1973-), male, from Sichuan Province, researcher, PhD, research direction: crop chemical control research, (E-mail)leib668@xaas.ac.cn;ZHOU Xiaoyun (1977-), male, Chongqing, researcher, PhD, research direction: crop chemical control and biotechnology, (E-mail)xiaoyunzhou77@126.com
Supported by:
Tianshan Innovation Team Program Project of Xinjiang Uygur Autonomous Region(2020D14001);Tianshan Cedar Project(2020XS16);S & T Assisting Xinjiang Project of Xinjiang Uygur Autonomous Region(2020E02093);Rural Revitalization Industry Development of Science and Technology Action Project of Xinjiang Uygur Autonomous Region(2020NC018)

16种植物甲醇提取物对小麦萌发及幼苗生长活性筛选与评价

郑瑞明¹^,²(), 王莉²^,³, 张军高¹^,³^,⁴, 鄢蓉², 李进¹^,³, 梁晶¹^,³, 雷斌¹^,³(), 周小云¹^,³()

1.新疆农业科学院核技术生物技术研究所/农业农村部荒漠绿洲作物生理生态与耕作重点实验室,乌鲁木齐 830091
2.新疆农业大学农学院,乌鲁木齐 830052
3.新疆作物化学调控工程技术研究中心, 乌鲁木齐 830091
4.新疆绿洲兴源农业科技有限责任公司,乌鲁木齐 830091

通讯作者: 雷斌(1973-),男,四川巴中人,研究员,博士,研究方向为农药研制及作物化控技术,(E-mail)leib668@xaas.ac.cn;周小云(1977-),男,重庆人,研究员,博士,研究方向为作物化控与生物技术,(E-mail)xiaoyunzhou77@126.com
作者简介:郑瑞明(1996-),男,安徽合肥人,硕士研究生,研究方向为农作物化控技术与产品,(E-mail)196685079@qq.com
基金资助:
新疆维吾尔自治区天山创新团队计划(2020D14001);天山雪松计划(2020XS16);新疆维吾尔自治区科技支疆计划(2020E02093);新疆维吾尔自治区乡村振兴产业发展科技行动(2020NC018)

Abstract

Abstract:

【Objective】 To explore medical plant resources, develop new pesticides, and promote green agricultural production, the regulatory effects of methanol extracts of 16 common medical plants on wheat germination and seedling growth were investigated, and new plant source extracts with good growth regulation activity were screened out and the appropriate concentration range was determined. 【Methods】 16 kinds of agricultural medicinal plant methanol extract were prepared into four different concentrations (10%, 1%, 0.1%, 0.01%) of the solution.Through germination test screening and potting test verification, the leaf length, stem thickness, dry and fresh weight of aboveground parts, endogenous hormone content and photosynthetic characteristics of wheat seedlings were determined, and the plant-derived extracts with the best effect were evaluated by the membership function method and principal component analysis method and after that the appropriate concentration range was determined. 【Results】 The results showed that the germination potential of 0.01%Sophraalopecuroides, Vallisnerianatans and Cuminumcyminum methanol extracts increased by 10.00%, 8.83% and 7.62%, respectively, and the root length increased by 23.44%, 21.88% and 18.75%, respectively.The comprehensive evaluation of seed germination experiments by membership function method was preliminarily screened out, 0.1% Sophraalopecuroides, 0.01% Sophraalopecuroides, 0.01% Cuminumcyminum, 1%Vallisnerianatans, 0.1%Vallisnerianatans, 0.01%Vallisnerianatans, 10%DaturametelL and 0.01%Euphorbia pekinensis extract, and further potting experiments verified that the leaf length of 0.01% Sophraalopecuroides and Vallisnerianatans extract increased by 16.62% and 15.91%, respectively, and the fresh weight of wheat treated with Cuminumcyminum and Sophraalopecuroides extract increased by 14.78% and 5.15%, respectively.The indexes were comprehensively sorted by principal component analysis, and the top three evaluation scores were 0.01%Sophraalopecuroides, 0.01%Vallisnerianatans and 0.01% Cuminumcyminum extract, which had certain growth promotion potential for wheat. 【Conclusion】 The three concentrations of methanol extracts of 0.01% Sophraalopecuroides, 0.01% Vallisnerianatansand 0.01% Cuminumcyminum have good effects, which can regulate the endogenous hormone level of wheat and promote the growth of seedlings.

Key words: wheat; methanol extract; activity screening.

摘要：

【目的】分析植物甲醇提取物对小麦萌发及幼苗生长的调节作用,筛选出生长调节活性较好的新型植物源提取物并确定适宜的浓度范围。【方法】以推广春麦品种新春44号为材料,以蒸馏水处理为对照,将16种农业药用植物甲醇提取物浸膏分别制备成4种不同浓度(10%、1%、0.1%、0.01%)的溶液。采用发芽试验筛选与盆栽试验验证,测定小麦幼苗的叶长、茎粗、地上部分干鲜重、内源激素含量以及光合特性等指标,运用隶属函数法与主成分分析法评价效果最优的植物源提取物并确定适宜的浓度范围。【结果】与对照比较,0.01%苦豆子、苦草和孜然甲醇提取物浸种发芽势分别增加10.00%、8.83%、7.62%;根长分别提高23.44%、21.88%、18.75%。筛选出0.1%苦豆子、0.01%苦豆子、0.01%孜然、1%苦草、0.1%苦草、0.01%苦草、10%曼陀罗和0.01%大戟提取物,0.01%苦豆子和苦草提取物叶长分别提高16.62%、15.91%;0.01%孜然与苦豆子提取物处理的小麦鲜重分别增加14.78%、5.15%。综合评价前3位分别为0.01%苦豆子、0.01%苦草和0.01%孜然提取物,对小麦具有一定的促生潜力。【结论】苦豆子、苦草、孜然3种低浓度甲醇提取物小麦种子萌发与幼苗生长效果较好,能够调控小麦内源激素水平,促进幼苗生长。

关键词: 小麦, 甲醇提取物, 活性筛选

CLC Number:

S482.3
S512

ZHENG Ruiming, WANG Li, ZHANG Jungao, YAN Rong, LI Jin, LIANG Jing, LEI Bin, ZHOU Xiaoyun. Screening and Evaluation of Wheat Germination and Seedling Growth Activity by 16 Plant Methanol Extracts[J]. Xinjiang Agricultural Sciences, 2023, 60(1): 32-42.

郑瑞明, 王莉, 张军高, 鄢蓉, 李进, 梁晶, 雷斌, 周小云. 16种植物甲醇提取物对小麦萌发及幼苗生长活性筛选与评价[J]. 新疆农业科学, 2023, 60(1): 32-42.

Figures/Tables 8

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科名 Familys	种名 Species	供试部位 Extraction parts	采集地点 Location
茄科Solanaceae	曼陀罗Datura metel	整株	轮台县
柏科Cupressaceae	线柏Chamaecyparis pisifera	枝叶	新和县
莎草科Cyperaceae	猪毛草Scirpus wallichii	茎叶	疏勒县
水鳖科Hydrocharitacea	苦草Vallisneria natans	枝叶	巴楚县
胡颓子科Elaeagnaceae	沙枣Elaeagnus angustifolia	枝叶	巴楚县
含羞草科Mimosaceae	金合欢Acacia dyepanolobium	枝叶	麦盖提县
藜科Chenopodiaceae	灰绿藜Chenopodium glaucum	整株	玛纳斯县
大戟科Euphorbiaceae	大戟Euphorbia pekinensis	茎叶	昭苏县
唇形科Labiatae	黄芩Scutellariabaicalensis	整株	玛纳斯县
唇形科Labiatae	牛至Organum vulgare	茎叶	昭苏县
蒺藜科Zygophyllaceae	小果白刺Nitraria sibirica	枝叶	库车县
伞形科Umbelliferae	毒参Hemlock	茎叶	昭苏县
伞形科Umbelliferae	孜然Cuminum cyminum	整株	玛纳斯县
豆科Hemlock	苦豆子Sophra alopecuroides	整株	玛纳斯县
	骆驼刺Alhagi sparsifoli	枝叶	疏勒县
	黄芪Astragali Radix	整株	玛纳斯县

科名 Familys	种名 Species	供试部位 Extraction parts	采集地点 Location
茄科Solanaceae	曼陀罗Datura metel	整株	轮台县
柏科Cupressaceae	线柏Chamaecyparis pisifera	枝叶	新和县
莎草科Cyperaceae	猪毛草Scirpus wallichii	茎叶	疏勒县
水鳖科Hydrocharitacea	苦草Vallisneria natans	枝叶	巴楚县
胡颓子科Elaeagnaceae	沙枣Elaeagnus angustifolia	枝叶	巴楚县
含羞草科Mimosaceae	金合欢Acacia dyepanolobium	枝叶	麦盖提县
藜科Chenopodiaceae	灰绿藜Chenopodium glaucum	整株	玛纳斯县
大戟科Euphorbiaceae	大戟Euphorbia pekinensis	茎叶	昭苏县
唇形科Labiatae	黄芩Scutellariabaicalensis	整株	玛纳斯县
唇形科Labiatae	牛至Organum vulgare	茎叶	昭苏县
蒺藜科Zygophyllaceae	小果白刺Nitraria sibirica	枝叶	库车县
伞形科Umbelliferae	毒参Hemlock	茎叶	昭苏县
伞形科Umbelliferae	孜然Cuminum cyminum	整株	玛纳斯县
豆科Hemlock	苦豆子Sophra alopecuroides	整株	玛纳斯县
	骆驼刺Alhagi sparsifoli	枝叶	疏勒县
	黄芪Astragali Radix	整株	玛纳斯县

处理Treatment	编号Number	处理Treatment	编号Number
10%苦豆子Sophra alopecuroides	Y1-1	10%小果白刺Nitraria sibirica	Y5-1
1%苦豆子Sophra alopecuroides	Y1-2	1%小果白刺Nitraria sibirica	Y5-2
0.1%苦豆子Sophra alopecuroides	Y1-3	0.1%小果白刺Nitraria sibirica	Y5-3
0.01%苦豆子Sophra alopecuroides	Y1-4	0.01%小果白刺Nitraria sibirica	Y5-4
10%孜然Cuminum cyminum	Y2-1	10%骆驼刺Alhagi sparsifoli	Y6-1
1%孜然Cuminum cyminum	Y2-2	1%骆驼刺Alhagi sparsifoli	Y6-2
0.1%孜然Cuminum cyminum	Y2-3	0.1%骆驼刺Alhagi sparsifoli	Y6-3
0.01%孜然Cuminum cyminum	Y2-4	0.01%骆驼刺Alhagi sparsifoli	Y6-4
10%苦草Vallisneria natans	Y3-1	10%大戟Euphorbia pekinensis	Y7-1
1%苦草Vallisneria natans	Y3-2	1%大戟Euphorbia pekinensis	Y7-2
0.1%苦草Vallisneria natans	Y3-3	0.1%大戟Euphorbia pekinensis	Y7-3
0.01%Vallisneria natans	Y3-4	0.01%大戟Euphorbia pekinensis	Y7-4
10%曼陀罗Datura metelL	Y4-1	10%黄芪Astragali Radix	Y8-1
1%曼陀罗Datura metelL	Y4-2	1%黄芪Astragali Radix	Y8-2
0.1%曼陀罗Datura metelL	Y4-3	0.1%黄芪Astragali Radix	Y8-3
0.01%曼陀罗Datura metelL	Y4-4	0.01%黄芪Astragali Radix	Y8-4
10%黄芩Astragali Radix	Y9-1	10%毒参Hemlock	Y13-1
1%黄芩Astragali Radix	Y9-2	1%毒参Hemlock	Y13-2
0.1%黄芩Astragali Radix	Y9-3	0.1%毒参Hemlock	Y13-3
0.01%黄芩Astragali Radix	Y9-4	0.01%毒参Hemlock	Y13-4
10%牛至Organum vulgare	Y10-1	10%金合欢Acacia dyepanolobium	Y14-1
1%牛至Organum vulgare	Y10-2	1%金合欢Acacia dyepanolobium	Y14-2
0.1%牛至Organum vulgare	Y10-3	0.1%金合欢Acacia dyepanolobium	Y14-3
0.01%牛至Organum vulgare	Y10-4	0.01%金合欢Acacia dyepanolobium	Y14-4
10%灰绿藜Chenopodium glaucum	Y11-1	10%猪毛草Scirpus wallichii	Y15-1
1%灰绿藜Chenopodium glaucum	Y11-2	1%猪毛草Scirpus wallichii	Y15-2
0.1%灰绿藜Chenopodium glaucum	Y11-3	0.1%猪毛草Scirpus wallichii	Y15-3
0.01%灰绿藜Chenopodium glaucum	Y11-4	0.01%猪毛草Scirpus wallichii	Y15-4
10%线柏Chamaecyparis pisifera	Y12-1	10%沙枣Elaeagnus angustifolia	Y16-1
1%线柏Chamaecyparis pisifera	Y12-2	1%沙枣Elaeagnus angustifolia	Y16-2
0.1%线柏Chamaecyparis pisifera	Y12-3	0.1%沙枣Elaeagnus angustifolia	Y16-3
0.01%线柏Chamaecyparis pisifera	Y12-4	0.01%沙枣Elaeagnus angustifolia	Y16-4

处理Treatment	编号Number	处理Treatment	编号Number
10%苦豆子Sophra alopecuroides	Y1-1	10%小果白刺Nitraria sibirica	Y5-1
1%苦豆子Sophra alopecuroides	Y1-2	1%小果白刺Nitraria sibirica	Y5-2
0.1%苦豆子Sophra alopecuroides	Y1-3	0.1%小果白刺Nitraria sibirica	Y5-3
0.01%苦豆子Sophra alopecuroides	Y1-4	0.01%小果白刺Nitraria sibirica	Y5-4
10%孜然Cuminum cyminum	Y2-1	10%骆驼刺Alhagi sparsifoli	Y6-1
1%孜然Cuminum cyminum	Y2-2	1%骆驼刺Alhagi sparsifoli	Y6-2
0.1%孜然Cuminum cyminum	Y2-3	0.1%骆驼刺Alhagi sparsifoli	Y6-3
0.01%孜然Cuminum cyminum	Y2-4	0.01%骆驼刺Alhagi sparsifoli	Y6-4
10%苦草Vallisneria natans	Y3-1	10%大戟Euphorbia pekinensis	Y7-1
1%苦草Vallisneria natans	Y3-2	1%大戟Euphorbia pekinensis	Y7-2
0.1%苦草Vallisneria natans	Y3-3	0.1%大戟Euphorbia pekinensis	Y7-3
0.01%Vallisneria natans	Y3-4	0.01%大戟Euphorbia pekinensis	Y7-4
10%曼陀罗Datura metelL	Y4-1	10%黄芪Astragali Radix	Y8-1
1%曼陀罗Datura metelL	Y4-2	1%黄芪Astragali Radix	Y8-2
0.1%曼陀罗Datura metelL	Y4-3	0.1%黄芪Astragali Radix	Y8-3
0.01%曼陀罗Datura metelL	Y4-4	0.01%黄芪Astragali Radix	Y8-4
10%黄芩Astragali Radix	Y9-1	10%毒参Hemlock	Y13-1
1%黄芩Astragali Radix	Y9-2	1%毒参Hemlock	Y13-2
0.1%黄芩Astragali Radix	Y9-3	0.1%毒参Hemlock	Y13-3
0.01%黄芩Astragali Radix	Y9-4	0.01%毒参Hemlock	Y13-4
10%牛至Organum vulgare	Y10-1	10%金合欢Acacia dyepanolobium	Y14-1
1%牛至Organum vulgare	Y10-2	1%金合欢Acacia dyepanolobium	Y14-2
0.1%牛至Organum vulgare	Y10-3	0.1%金合欢Acacia dyepanolobium	Y14-3
0.01%牛至Organum vulgare	Y10-4	0.01%金合欢Acacia dyepanolobium	Y14-4
10%灰绿藜Chenopodium glaucum	Y11-1	10%猪毛草Scirpus wallichii	Y15-1
1%灰绿藜Chenopodium glaucum	Y11-2	1%猪毛草Scirpus wallichii	Y15-2
0.1%灰绿藜Chenopodium glaucum	Y11-3	0.1%猪毛草Scirpus wallichii	Y15-3
0.01%灰绿藜Chenopodium glaucum	Y11-4	0.01%猪毛草Scirpus wallichii	Y15-4
10%线柏Chamaecyparis pisifera	Y12-1	10%沙枣Elaeagnus angustifolia	Y16-1
1%线柏Chamaecyparis pisifera	Y12-2	1%沙枣Elaeagnus angustifolia	Y16-2
0.1%线柏Chamaecyparis pisifera	Y12-3	0.1%沙枣Elaeagnus angustifolia	Y16-3
0.01%线柏Chamaecyparis pisifera	Y12-4	0.01%沙枣Elaeagnus angustifolia	Y16-4

种名 Species	提取率 Extraction rate(%)
灰绿藜Oakleaf Goosefoot	15.21
曼陀罗Datura metelL	11.30
线柏Chamaecyparis pisifera	13.76
猪毛草Scirpus wallichii	15.40
苦草Vallisneria natans	26.20
沙枣Elaeagnus angustifolia	14.37
金合欢Acacia dyepanolobium	12.29
大戟Euphorbia pekinensis	19.45
黄芩Scutellariabaicalensis	18.13
牛至Organum vulgare	21.14
小果白刺Nitraria sibirica	15.80
毒参Hemlock	19.70
孜然Cuminum cyminum	12.87
苦豆子Sophra alopecuroides	12.61
骆驼刺Alhagi sparsifoli	6.11
黄芪Astragali Radix	17.00

Screening and Evaluation of Wheat Germination and Seedling Growth Activity by 16 Plant Methanol Extracts

16种植物甲醇提取物对小麦萌发及幼苗生长活性筛选与评价

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处理 Treatment	发芽势 Germination potential (%)	发芽率 Germination rate (%)	苗高 Shoot height	根长 Root length	根数 Root number	储藏物质转运率 Storage transport rate(%)	综合评价值D D value
CK	82.7^bcd	89.0^bc	7.8^bc	6.4^bc	5.4^bc	25.1^bc	0.65
Y1-1	50.7^g	71.0^ef	5.3^d	5.4^d	4.8^cd	15.9^e	0.40
Y1-2	81.3^bc	89.0^bc	6.3^cd	6.3^bc	5.4^bc	20.6^d	0.57
Y1-3	84.0^bc	89.0^bc	8.7^ab	7.0^bc	6.0^c	27.3^b	0.71
Y1-4	91.0^a	95.0^a	9.2^a	7.9^a	6.4^a	30.2^a	0.93
Y2-1	12.7^ef	24.0^g	4.4^de	4.3^de	4.6^cd	12.8^e	0.14
Y2-2	82.7^bc	89.0^bc	6.5^cd	6.7^bc	5.4^bc	24.1^bc	0.62
Y2-3	84.0^bc	86.0^cd	8.8^ab	6.3^b	4.9^cd	31.8^a	0.63
Y2-4	89.0^a	92.0^a	8.9^a	7.6^ab	6.5^a	23.8^d	0.86
Y3-1	70.0^de	84.0^cd	7.1^bc	7.3^ab	6.3^ab	20.8^d	0.67
Y3-2	83.0^bcd	89.0^bc	10.6^a	6.8^bc	6.3^ab	25.8^bc	0.80
Y3-3	84.0^bc	87.0^bc	8.1^ab	7.0^bc	6.3^ab	33.6^a	0.73
Y3-4	90.0^a	93.0^a	9.1^a	7.8^ab	5.9^bc	27.7^ab	0.77
Y4-1	84.6^bc	88.2^bc	7.9^bc	6.7^bc	6.3^ab	22.8^cd	0.71
Y4-2	83.0^bcd	89.4^ab	7.3^bc	6.8^bc	5.8^bc	21.3^d	0.67
Y4-3	86.0^ab	88.2^bc	7.5^bc	5.4^cd	6.2^ab	23.3^cd	0.61
Y4-4	86.0^ab	88.8^bc	7.9^bc	5.7^cd	5.9^bc	27.7^ab	0.65
Y5-1	75.0^de	70.0^ef	5.4^d	5.0^cd	4.1^d	15.3^e	0.41
Y5-2	82.8^bc	75.3^de	6.0^cd	5.2^cd	5.0^c	26.6^b	0.46
Y5-3	85.2^bc	88.7^bc	6.4^cd	4.7^de	4.6^cd	18.8^de	0.49
Y5-4	84.0^bc	87.0^bc	7.8^bc	6.5^bc	5.5^bc	26.8^b	0.58
Y6-1	74.4^de	68.7^ef	5.4^d	5.9^cd	3.7^de	20.7^d	0.38
处理 Treatment	发芽势 Germination potential (%)	发芽率 Germination rate (%)	苗高 Shoot height	根长 Root length	根数 Root number	储藏物质转运率 Storage transport rate(%)	综合评价值D D value
Y6-2	79.2^cd	80.0^de	6.6^cd	5.6^cd	5.0^c	26.3^bc	0.53
Y6-3	78.6^bc	84.7^cd	7.6^bc	5.6^cd	4.9^cd	26.6^b	0.55
Y6-4	75.0^cd	81.3^cd	5.7^d	6.3^bc	4.8^cd	25.9^bc	0.46
Y7-1	45.0^ef	66.0^ef	6.2^cd	5.9^cd	5.4^bc	22.4^d	0.45
Y7-2	61.2^ef	79.3^de	7.6^bc	6.7^bc	6.2^ab	27.5^b	0.57
Y7-3	70.8^de	80.0^de	6.4^cd	6.8^bc	5.8^bc	27.2^b	0.55
Y7-4	86.0^ab	89.3^ab	8.7^ab	6.7^bc	6.0^b	26.8^bc	0.70
Y8-1	59.4^ef	80.0^de	6.0^cd	6.0^c	5.5^bc	29.7^ab	0.52
Y8-2	68.4^de	86.0^cd	7.6^bc	6.4^bc	5.8^bc	28.3^ab	0.62
Y8-3	69.0^de	86.3^bc	7.1^bc	6.7^bc	6.0^b	26.1^bc	0.63
Y9-1	61.8^ef	87.3^bc	7.3^bc	6.6^bc	5.8^bc	26.5^bc	0.62
Y9-2	81.0^bc	88.9^bc	7.6^bc	6.6^bc	5.9^c	23.4^cd	0.68
Y9-3	81.0^bc	87.6^bc	7.8^bc	7.1^ab	6.1^b	27.9^ab	0.63
Y9-4	79.8^cd	84.9^cd	7.6^bc	6.8^bc	5.6^bc	26.9^bc	0.60
Y10-1	74.4^de	87.3^bc	5.4^d	7.3^ab	5.4^bc	20.1^d	0.58
Y10-2	78.6^cd	87.7^bc	6.7^cd	7.0^bc	5.8^bc	25.5^bc	0.53
Y10-3	81.6^bc	89.3^ab	7.7^bc	5.4^d	5.9^bc	26.3^bc	0.56
Y10-4	77.4^cd	86.3^bc	7.1^c	6.8^bc	5.5^bc	24.9^bc	0.54
Y11-1	57.6^ef	77.3^de	7.3^bc	6.6^bc	5.6^bc	23.1^cd	0.46
Y11-2	78.0^cd	89.3^ab	7.9^bc	5.8^cd	5.5^bc	27.3^b	0.56
Y11-3	79.8^cd	88.7^bc	8.0^ab	6.5^bc	5.5^bc	23.5^cd	0.59
Y11-4	85.6^bc	87.4^bc	8.4^ab	6.8^bc	5.7^bc	27.4^b	0.45
Y12-1	53.4^ef	62.1^ef	4.3^de	5.5^cd	5.6^bc	21.5^d	0.42
Y12-2	72.0^de	81.1^cd	7.5^bc	6.0^c	5.6^bc	23.8^bc	0.50
Y12-3	76.8^de	86.8^bc	8.3^ab	4.9^de	6.0^c	28.4^ab	0.53
Y12-4	82.2^bc	90.3^ab	8.6^ab	5.7^cd	5.4^bc	28.1^ab	0.46
Y13-1	55.8^ef	59.5^ef	5.8^d	6.6^bc	4.3^cd	21.6^d	0.43
Y13-2	77.4^cd	79.8^de	7.4^bc	6.4^bc	5.7^bc	29.6^ab	0.52
Y13-3	75.6^de	88.0^bc	8.2^ab	5.1^de	6.1^b	29.2^ab	0.54
Y13-4	78.0^cd	88.0^bc	7.3^bc	6.7^bc	5.5^bc	28.6^ab	0.45
Y14-1	76.0^cd	66.0^ef	6.3^cd	5.2^de	5.1^c	22.6^c	0.47
Y14-2	78.0^cd	88.0^bc	8.3^ab	5.3^d	5.7^bc	28.1^ab	0.53
Y14-3	81.6^de	89.3^ab	8.0^ab	6.2^bc	5.2^c	26.0^b	0.58
Y14-4	79.2b^c	87.3^bc	8.0^ab	6.6^bc	5.7^bc	28.5^ab	0.52
Y15-1	57.0^ef	75.3^de	5.7^d	6.6^bc	5.7^bc	20.4^d	0.52
Y15-2	79.2^cd	86.8^bc	8.1^ab	6.7^bc	6.0^b	27.6^ab	0.54
Y15-3	78.6^cd	88.7^bc	8.1^ab	5.8^cd	5.7^bc	29.4^ab	0.58
Y15-4	82.8^bc	85.5^cd	8.0^ab	6.7^bc	5.7^bc	27.2^b	0.49
Y16-1	64.2^de	72.8^ef	5.3^d	6.6^bc	5.4^bc	20.4^d	0.46
Y16-2	83.4^bc	88.1^bc	6.9^cd	6.2^bc	5.6^bc	27.5^b	0.56
Y16-3	80.4^bc	88.1^bc	7.8^bc	6.0^c	5.8^bc	29.1^ab	0.55
Y16-4	82.8^bc	88.8^bc	7.3^bc	6.7^bc	5.5^bc	25.2^bc	0.66

处理 Treatment	净光合速率Pn Net photosyn thesis rate Pn (μmol/(m²·S))	蒸腾速率Tr Transpiration rate Tr (mmol/(m²·S))	气孔导度Gs Stomatal conductance Gs (mol/(m²·S))	胞间CO₂浓度Ci Intercellular CO₂ concentration Ci (μmol/mol)	气孔限制值 Stomatal limiation	SPAD值 SPAD value
CK	6.51±0.71^b	2.12±0.28^a	0.11±0.01^ab	300.01±15.23^ab	0.41^a	38.65±2.48^b
Y1-3	5.50±0.73^c	2.30±0.28^a	0.09±0.02^b	285.03±15.48^c	0.42^a	40.25±2.31^ab
Y1-4	7.82±0.68^a	2.23±0.31^a	0.11±0.01^ab	312.24±14.69^a	0.41^a	41.08±2.34^a
Y2-4	6.92±0.74^a	2.30±0.29^a	0.13±0.03^ab	294.62±15.46^b	0.37^a	40.57±2.48a^b
Y3-2	6.27±0.72^b	2.50±0.34^a	0.15±0.01^a	289.45±16.35^bc	0.38^a	38.70±3.64^ab
Y3-3	6.22±0.81^b	2.34±0.31^a	0.08±0.03^b	286.53±15.21^bc	0.39^a	35.70±2.74^c
Y3-4	6.93±0.74^a	2.40±0.32^a	0.12±0.04^a	298.63±14.86^b	0.42^a	40.00±2.54^ab
Y4-1	6.41±0.73^b	2.65±0.33^a	0.11±0.01^ab	295.63±15.36^b	0.43^a	35.86±3.80^b
Y7-4	6.31±0.73^b	2.70±0.31^a	0.13±0.02^a	307.56±16.35^a	0.41^a	39.20±3.85^ab

处理 Treat- ment	因子Factor		F₁	F₂	F	排序 Order
处理 Treat- ment	因子1 Factor 1	因子2 Factor 2	F₁	F₂	F	排序 Order
CK	-0.07	-0.47	-0.15	-0.57	-0.25	5
Y1-3	0.39	-1.61	0.85	-1.93	0.20	4
Y1-4	1.25	2.05	2.72	2.47	2.66	1
Y2-4	1.03	0.01	2.25	0.02	1.74	3
Y3-2	-1.22	0.50	-2.66	0.60	-1.90	8
Y3-3	-1.28	-0.06	-2.80	-0.07	-2.17	9
Y3-4	1.12	0.49	2.45	0.59	2.02	2
Y4-1	-0.39	-0.64	-0.85	-0.77	-0.83	6
Y7-4	-0.83	-0.27	-1.82	-0.32	-1.47	7

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