Xinjiang Agricultural Sciences ›› 2022, Vol. 59 ›› Issue (4): 1034-1040.DOI: 10.6048/j.issn.1001-4330.2022.04.030
• Genetic Engineering·Prataculture·Agricultural Product Analysis and Detection·Animal Husbandry Veterinarian • Previous Articles
LI Hongbo1,2(), LI Na3(), YAN Xiangmin1,2, YE Zhibing1,2, CUI Fanrong1,2, ZHANG Yang1,2
Received:
2021-03-11
Online:
2022-04-20
Published:
2022-04-24
Correspondence author:
LI Na
Supported by:
李红波1,2(), 李娜3(), 闫向民1,2, 叶治兵1,2, 崔繁荣1,2, 张杨1,2
通讯作者:
李娜
作者简介:
李红波(1980-),男,新疆人,副研究员,硕士,研究方向为肉牛遗传育种与饲养管理,(E-mail) lihb8228@126.com
基金资助:
CLC Number:
LI Hongbo, LI Na, YAN Xiangmin, YE Zhibing, CUI Fanrong, ZHANG Yang. Correlation between Leptin Gene Expression Level in Different Tissues and Meat Quality in Xinjiang Brown Cattle[J]. Xinjiang Agricultural Sciences, 2022, 59(4): 1034-1040.
李红波, 李娜, 闫向民, 叶治兵, 崔繁荣, 张杨. 新疆褐牛瘦素基因在不同部位中表达水平与肉质指标的关联性[J]. 新疆农业科学, 2022, 59(4): 1034-1040.
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URL: http://www.xjnykx.com/EN/10.6048/j.issn.1001-4330.2022.04.030
基因名称 Gene Name | 方向 Direc tion | 序列(5'-3') Sequence(5'-3') | 片段 大小 Frag ment size (bp) |
---|---|---|---|
gapdh | F | TCATAGACAAGATGGTGAAGGTC | 163 |
R | TGGGTAGAGTCATACTGGAACAT | ||
leptin | F | AGACACCGTGAAAAGGAGA | 207 |
R | CACTGAATGTTTGTGGAATG |
Table 1 The primer sequence information
基因名称 Gene Name | 方向 Direc tion | 序列(5'-3') Sequence(5'-3') | 片段 大小 Frag ment size (bp) |
---|---|---|---|
gapdh | F | TCATAGACAAGATGGTGAAGGTC | 163 |
R | TGGGTAGAGTCATACTGGAACAT | ||
leptin | F | AGACACCGTGAAAAGGAGA | 207 |
R | CACTGAATGTTTGTGGAATG |
Fig.1 The expression level of leptin gene in the adipose tissues of various parts of Xinjiang brown cattle Note:** indicates that it is highly statistically significant at P<0.01
性状Traits | 性状定义 Definition of character | 平均值(n=20) Average value |
---|---|---|
活体重Live weight (kg) | 禁食24 h后临宰前的实际体重 | 620.15±74.44 |
体高Height(cm) | 从鬐甲最高点到地面的垂直距离 | 142.81±22.04 |
BPI | 体重与体高的比值 | 4.45±0.63 |
胴体重Carcass weight (kg) | 除去头蹄下水后带骨肉(含板油和肾)的重量 | 367.95±45.92 |
净肉率Net meat percentage (%) | 宰后胴体重量与活重(空腹24 h)的比率 | 59.33±1.01 |
眼肌面积Eye muscle area (cm2) | 1213肋的横断面的面积。 | 95.23±15.24 |
蒸煮损失Cooking loss (%) | 生肉加工成熟肉过程中质量减少的百分比 | 23.70±8.24 |
嫩度Tenderness (kg) | 背最长肌的剪切力 | 3.32±0.84 |
脂肪Fat (%) | 背最长肌脂肪所占比例 | 5.17±0.43 |
蛋白质Protein (%) | 背最长肌蛋白质所占比例 | 23.11±0.47 |
水分Moisture (%) | 背最长肌水分所占比例 | 71.82±1.38 |
Table 2 Meat quality characteristics of longissimus dorsi muscle tissue of Xinjiang brown cattle
性状Traits | 性状定义 Definition of character | 平均值(n=20) Average value |
---|---|---|
活体重Live weight (kg) | 禁食24 h后临宰前的实际体重 | 620.15±74.44 |
体高Height(cm) | 从鬐甲最高点到地面的垂直距离 | 142.81±22.04 |
BPI | 体重与体高的比值 | 4.45±0.63 |
胴体重Carcass weight (kg) | 除去头蹄下水后带骨肉(含板油和肾)的重量 | 367.95±45.92 |
净肉率Net meat percentage (%) | 宰后胴体重量与活重(空腹24 h)的比率 | 59.33±1.01 |
眼肌面积Eye muscle area (cm2) | 1213肋的横断面的面积。 | 95.23±15.24 |
蒸煮损失Cooking loss (%) | 生肉加工成熟肉过程中质量减少的百分比 | 23.70±8.24 |
嫩度Tenderness (kg) | 背最长肌的剪切力 | 3.32±0.84 |
脂肪Fat (%) | 背最长肌脂肪所占比例 | 5.17±0.43 |
蛋白质Protein (%) | 背最长肌蛋白质所占比例 | 23.11±0.47 |
水分Moisture (%) | 背最长肌水分所占比例 | 71.82±1.38 |
项目 Index | 皮下脂肪中leptin 表达水平 The expression level of leptin in subcutaneous fat | 肾周脂肪中leptin 表达水平 The expression level of leptin in peripheral fat | 肌间脂肪中leptin 表达水平 The expression level of leptin in intermuscular fat | 心周脂肪中leptin 表达水平 The expression level of leptin in pericardial fat |
---|---|---|---|---|
大理石花纹Marble pattern | -0.217 | 0.119 | 0.029 | 0.126 |
活体重Live weight | -0.216 | -0.251 | 0.252 | -0.111 |
脂肪覆盖等级Fat coverage grade | 0.182 | -0.283 | -0.238 | -0.191 |
BPI | -0.249 | -0.376 | 0.251 | 0.137 |
pH | -0.399 | 0.268 | -0.091 | 0.106 |
剪切力Shear force | 0.555* | -0.017 | 0.363 | 0.277 |
蒸煮损失Cooking loss | 0.479* | 0.084 | 0.134 | -0.059 |
肌内脂肪含量Intramuscular fat content | 0.474* | -0.088 | -0.435 | -0.252 |
Table 3 Correlation between leptin gene expression levels in different parts and meat quality traits
项目 Index | 皮下脂肪中leptin 表达水平 The expression level of leptin in subcutaneous fat | 肾周脂肪中leptin 表达水平 The expression level of leptin in peripheral fat | 肌间脂肪中leptin 表达水平 The expression level of leptin in intermuscular fat | 心周脂肪中leptin 表达水平 The expression level of leptin in pericardial fat |
---|---|---|---|---|
大理石花纹Marble pattern | -0.217 | 0.119 | 0.029 | 0.126 |
活体重Live weight | -0.216 | -0.251 | 0.252 | -0.111 |
脂肪覆盖等级Fat coverage grade | 0.182 | -0.283 | -0.238 | -0.191 |
BPI | -0.249 | -0.376 | 0.251 | 0.137 |
pH | -0.399 | 0.268 | -0.091 | 0.106 |
剪切力Shear force | 0.555* | -0.017 | 0.363 | 0.277 |
蒸煮损失Cooking loss | 0.479* | 0.084 | 0.134 | -0.059 |
肌内脂肪含量Intramuscular fat content | 0.474* | -0.088 | -0.435 | -0.252 |
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