基于分子检测新疆东部棉花根茎部病害种类及其分布

doi:10.6048/j.issn.1001-4330.2017.11.011

新疆农业科学 ›› 2017, Vol. 54 ›› Issue (11): 2046-2053.DOI: 10.6048/j.issn.1001-4330.2017.11.011

基于分子检测新疆东部棉花根茎部病害种类及其分布

马依努尔·米吉提, 族米娜, 萨吉达·艾孜则, 郭庆元

新疆农业大学/新疆农业大学农学院农林有害生物监测与安全防控重点实验室,乌鲁木齐 830052

收稿日期:2017-11-01 出版日期:2017-11-20 发布日期:2017-12-06
通信作者: 郭庆元(1962-),四川人,教授,硕士生导师,研究方向为植物病理,(E-mail)guoqingyuan3009@sina.com
作者简介:马依努尔·米吉提(1991-),新疆人,硕士研究生,研究方向为植物病理,(E-mail)2517884144@qq.com
基金资助:
农业部专项“作物根腐类病害防控技术研究与集成”(201503112);新疆维吾尔自治区重点研发计划“棉田主要病虫防治及农药减施增效新技术与产品研发”(2016B01001)

Species and Distribution of Cotton Root and Stem Diseases in Eastern Xinjiang Based on Molecular Detection

Mayinuer Mijiti, Zumina, Sajida Aizizi, GUO Qing -yuan

Xinjiang Agricultural University / College of Agronomy / Key Laboratory of the Agriculture and Forestry Pest Monitoring and Safety Control of College of Agronomy, Xinjiang Agricultural University, Urumqi 830052, China

Received:2017-11-01 Online:2017-11-20 Published:2017-12-06
Correspondence author: GUO Qing-yuan(1962-),Sichuan people, Professor of college of Agronomy, Xinjiang Agricultural University, working on plant pathology,(E-mail)guoqingyuan3009@sina.com
Supported by:
Special projects of Ministry of Agriculture “Research and integration of crop root rot disease control technology”(201503112);Key research and development plans of the xinjiang uygur autonomous region “Development of new technology and product for control Main diseases and pests and reduce Pesticide and increase effect in cotton field”(2016B01001)

摘要/Abstract

摘要： 【目的】研究新疆东部棉花根茎部病害的种类及分布,为当地高效防控此类病害提供依据。【方法】采用已报道的4种引起棉花根茎部病害的主要病原菌(尖孢镰孢霉、立枯丝核菌、大丽轮枝菌和拟轮枝镰孢霉)的特异性引物,特异性验证后,分别对新疆东部棉花区按不同以村(连)为单位采集的混合病样进行PCR检测。【结果】在检测灵敏度为0.5 ng/μL的检测体系中,从22份新疆东部棉花病株混合样品中可以检测出4种病原菌中的3种,分别为尖孢镰孢霉、立枯丝核菌、大丽轮枝菌;其中尖孢镰刀菌检出率最高,立枯丝核菌检出率次之,大丽轮枝菌检出率较低;而拟轮枝镰孢霉在22份混合病样中的检出率0%。【结论】枯萎病、立枯病和黄萎病是新疆东部根茎部主要病害;其中枯萎病的发生最为普遍,立枯病次之,黄萎病发生的普遍率相对较低,红腐病在新疆东部无发生或发生分布极少。

关键词: 棉花; 根茎部病害; PCR检测; 病害分布

Abstract: 【Objective】 Clarifying species and distribution of cotton root and stem diseases in eastern Xinjiang can provide a basis for effective prevention and control of such diseases in the local area.【Method】The reported specific primers of the 4 species pathogens (Fusarium oxysporum, Rhizoctonia solani, verticillium dahliae and Fusarium verticilliodes) which cause the root and stem disease of cotton are used for the specific verification, and carrying out PCR detection for the mixed disease samples of the collected according to different villages (townships) from the cotton areas in Eastern Xinjiang.【Result】In the tested system with sensitivity of 0.5 ng/μL, 3 species of 4 pathogenic bacteria were detected from the 22 mixed samples of cotton infected plants from the Eastern Xinjiang. They were respectively Fusarium oxysporum, Rhizoctonia solani and verticillium dahliae;Among them, Fusarium oxysporum had the highest detection rate,Rhizoctonia solani was the second, V. dahliae dad the lower detection rate. But, the detection rate ofFusarium verticilliodes was 0% in the 22 mixed samples.【Conclusion】The detection results indicated that the Fusarium wilt,Rhizoctonia damping-off and Verticillium yellow wilt were the main root and stem diseases of cotton in Eastern Xinjiang; Among which, Fusarium wilt is the most common one, followed by Rhizoctonia damping-off, and the incidence of Verticillium wilt is relatively low. In Eastern Xinjiang, red rot has not occurred or distribute very little.

Key words: cotton; root and stem disease; PCR detection; distribution of disease

中图分类号:

S435.621

马依努尔·米吉提, 族米娜, 萨吉达·艾孜则, 郭庆元. 基于分子检测新疆东部棉花根茎部病害种类及其分布[J]. 新疆农业科学, 2017, 54(11): 2046-2053.

Mayinuer Mijiti, Zumina, Sajida Aizizi, GUO Qing -yuan. Species and Distribution of Cotton Root and Stem Diseases in Eastern Xinjiang Based on Molecular Detection[J]. Xinjiang Agricultural Sciences, 2017, 54(11): 2046-2053.

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基于分子检测新疆东部棉花根茎部病害种类及其分布

Species and Distribution of Cotton Root and Stem Diseases in Eastern Xinjiang Based on Molecular Detection

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