新疆农业科学 ›› 2023, Vol. 60 ›› Issue (5): 1263-1270.DOI: 10.6048/j.issn.1001-4330.2023.05.026
王宁1,2(), 史应武1,2, 牛新湘2,3, 杨红梅1,2, 楚敏1,2, 詹发强1, 包慧芳1, 杨蓉1, 龙宣杞1, 丁荣荣4()
收稿日期:
2022-08-28
出版日期:
2023-05-20
发布日期:
2023-05-22
通信作者:
丁荣荣(1978-),女,新疆呼图壁人,助理研究员,研究方向为作物病虫害防治, (E-mail) 作者简介:
王宁(1979-),女,山东人,副研究员,研究方向为微生物发酵, (E-mail) 150700126@qq.com
基金资助:
WANG Ning1,2(), SHI Yingwu1,2, NIU Xinxiang2,3, YANG Hongmei1,2, CHU Min1,2, ZHAN Faqiang1, BAO Huifang1, YANG Rong1, LONG Xuanqi1, DING Rongrong4()
Received:
2022-08-28
Online:
2023-05-20
Published:
2023-05-22
Correspondence author:
DING Rongrong (1978-), female, from Hutobi, Xinjiang, associate researcher, research direction is extermination of disease and insect pest, (E-mail) Supported by:
摘要:
【目的】研究棉花根际溶磷菌WJP-7发酵培养基优化及防病增产效果。【方法】采用实验室筛选的高效溶磷菌株P.taiwanensis WJP-7为出发菌株,在优化的假单胞菌发酵培养基上增殖培养。【结果】利用正交试验优化摇瓶发酵培养基,培养基配方为蛋白胨 20 g/L,MgCl2 15 g/L,K2SO4 1.4 g/L。使活菌数达到3.6×109CFU/mL。摇瓶增菌发酵最优培养基发酵验证试验结果最高活菌数为3.60×109CFU/mL。菌株WJP-7水剂处理对花期黄萎病防治效果为44.57%,絮期防治效果为50.53%。施用WJP-7水剂对棉田土壤除全氮含量增加不显著,对其它养分和pH值均有显著影响(P<0.05)。菌剂增产率为14.35%和19.51%,【结论】优化了台湾假单胞菌WJP-7摇瓶发酵培养基,菌株WJP-7水剂处理对棉花黄萎病防治效果为50.53%,对棉田土壤除全氮含量不显著外,对其它养分和pH值均显著影响(P<0.05),具有明显的增产效果。
中图分类号:
王宁, 史应武, 牛新湘, 杨红梅, 楚敏, 詹发强, 包慧芳, 杨蓉, 龙宣杞, 丁荣荣. 棉花根际溶磷菌WJP-7发酵培养基优化及防病增产效果[J]. 新疆农业科学, 2023, 60(5): 1263-1270.
WANG Ning, SHI Yingwu, NIU Xinxiang, YANG Hongmei, CHU Min, ZHAN Faqiang, BAO Huifang, YANG Rong, LONG Xuanqi, DING Rongrong. Inoculation technology and field application of cotton rhizosphere phosphorus-solubilizing P.Taiwanensis WJP-7[J]. Xinjiang Agricultural Sciences, 2023, 60(5): 1263-1270.
因素 Factor | A蛋白胨 A Peptone (g/L) | B氯化镁 B Mgcl2 (g/L) | C硫酸钾 C K2SO4 (g/L) |
---|---|---|---|
Level 1 | 5 | 5 | 0.7 |
Level 2 | 10 | 10 | 1.4 |
Level 3 | 20 | 15 | 2.1 |
表1 因素水平
Tab.1 the table of factor level
因素 Factor | A蛋白胨 A Peptone (g/L) | B氯化镁 B Mgcl2 (g/L) | C硫酸钾 C K2SO4 (g/L) |
---|---|---|---|
Level 1 | 5 | 5 | 0.7 |
Level 2 | 10 | 10 | 1.4 |
Level 3 | 20 | 15 | 2.1 |
试验号 Test number | A | B | C | OD600 |
---|---|---|---|---|
1 | 1 | 1 | 1 | 0.53 |
2 | 1 | 2 | 2 | 0.60 |
3 | 1 | 3 | 3 | 0.58 |
4 | 2 | 1 | 2 | 1.23 |
5 | 2 | 2 | 3 | 1.30 |
6 | 2 | 3 | 1 | 1.34 |
7 | 3 | 1 | 3 | 1.58 |
8 | 3 | 2 | 1 | 1.70 |
9 | 3 | 3 | 2 | 1.83 |
K1 | 0.57 | 1.11 | 1.19 | |
K2 | 1.29 | 1.20 | 1.22 | |
K3 | 1.70 | 1.25 | 1.15 | |
R | 1.14 | 0.14 | 0.07 |
表2 L9(34)正交试验
Tab.2 L9(34)Orthogonal experimental results
试验号 Test number | A | B | C | OD600 |
---|---|---|---|---|
1 | 1 | 1 | 1 | 0.53 |
2 | 1 | 2 | 2 | 0.60 |
3 | 1 | 3 | 3 | 0.58 |
4 | 2 | 1 | 2 | 1.23 |
5 | 2 | 2 | 3 | 1.30 |
6 | 2 | 3 | 1 | 1.34 |
7 | 3 | 1 | 3 | 1.58 |
8 | 3 | 2 | 1 | 1.70 |
9 | 3 | 3 | 2 | 1.83 |
K1 | 0.57 | 1.11 | 1.19 | |
K2 | 1.29 | 1.20 | 1.22 | |
K3 | 1.70 | 1.25 | 1.15 | |
R | 1.14 | 0.14 | 0.07 |
因素 Factor | III型 平方和 Type III of sum squares | 自由度 DOF | 均方 Mean square | F | P |
---|---|---|---|---|---|
蛋白胨 Peptone | 1.974 | 2 | 0.987 | 420.929 | 0.002 |
氯化镁 Magnesium chloride | 0.029 | 2 | 0.014 | 6.118 | 0.140 |
硫酸钾 Potassium sulfate | 0.007 | 2 | 0.003 | 1.427 | 0.412 |
误差 Error | 0.005 | 2 | 0.002 |
表3 方差分析
Tab.3 Analysis of variance
因素 Factor | III型 平方和 Type III of sum squares | 自由度 DOF | 均方 Mean square | F | P |
---|---|---|---|---|---|
蛋白胨 Peptone | 1.974 | 2 | 0.987 | 420.929 | 0.002 |
氯化镁 Magnesium chloride | 0.029 | 2 | 0.014 | 6.118 | 0.140 |
硫酸钾 Potassium sulfate | 0.007 | 2 | 0.003 | 1.427 | 0.412 |
误差 Error | 0.005 | 2 | 0.002 |
处理 Treatment | 病指Disease index | 防效Control effect(%) | ||
---|---|---|---|---|
花期 Flowering period | 絮期 Boll opening period | 花期 Flowering period | 絮期 Boll opening period | |
30×108 CFU/mL WJP-7水剂 30×108CFU/mL WJP-7 Water agent | 2.51 | 3.26 | 44.57 | 50.53 |
65×108 CFU/g芽孢杆菌可湿性粉剂 65×108CFU/g Bacillus wettable powder | 1.12 | 2.45 | 75.22 | 62.82 |
清水Fresh water | 4.52 | 6.59 |
表4 不同处理对棉花黄萎病的防治效果比较
Tab.4 Effect of different treatments on cotton Verticillium wilt
处理 Treatment | 病指Disease index | 防效Control effect(%) | ||
---|---|---|---|---|
花期 Flowering period | 絮期 Boll opening period | 花期 Flowering period | 絮期 Boll opening period | |
30×108 CFU/mL WJP-7水剂 30×108CFU/mL WJP-7 Water agent | 2.51 | 3.26 | 44.57 | 50.53 |
65×108 CFU/g芽孢杆菌可湿性粉剂 65×108CFU/g Bacillus wettable powder | 1.12 | 2.45 | 75.22 | 62.82 |
清水Fresh water | 4.52 | 6.59 |
样品 Specimen | 有机质 Organic matter (g/kg) | 全氮 Total nitrogen (g/kg) | 全磷 Total phos- phorus (g/kg) | 全钾 Total potassium (g/kg) | 碱解氮 Alkaline hydrolysis nitrogen (mg/kg) | 速效磷 Rapidly available phosphorus (mg/kg) | 速效钾 Rapidly available potassium (mg/kg) | pH值 | 总盐 Total salt (g/kg) |
---|---|---|---|---|---|---|---|---|---|
T1 | 17.54a | 0.89a | 1.19a | 20.70a | 72.24b | 40.93a | 443.83a | 6.99a | 3.28b |
T2 | 17.95a | 0.92a | 1.14a | 20.82a | 75.39a | 35.26b | 445.13a | 7.02a | 3.15b |
CK | 14.46b | 0.82a | 0.91b | 20.03c | 63.12b | 28.15c | 404.59b | 6.70b | 6.80a |
表5 不同菌剂下棉田土壤理化性质变化
Tab.5 Effects of bacterial agents on the physicochemical properties of soil in cotton fields
样品 Specimen | 有机质 Organic matter (g/kg) | 全氮 Total nitrogen (g/kg) | 全磷 Total phos- phorus (g/kg) | 全钾 Total potassium (g/kg) | 碱解氮 Alkaline hydrolysis nitrogen (mg/kg) | 速效磷 Rapidly available phosphorus (mg/kg) | 速效钾 Rapidly available potassium (mg/kg) | pH值 | 总盐 Total salt (g/kg) |
---|---|---|---|---|---|---|---|---|---|
T1 | 17.54a | 0.89a | 1.19a | 20.70a | 72.24b | 40.93a | 443.83a | 6.99a | 3.28b |
T2 | 17.95a | 0.92a | 1.14a | 20.82a | 75.39a | 35.26b | 445.13a | 7.02a | 3.15b |
CK | 14.46b | 0.82a | 0.91b | 20.03c | 63.12b | 28.15c | 404.59b | 6.70b | 6.80a |
处理 Treatment | 单位面积株数/株 Number of plants/667m2 | 单铃重 Single boll weight(g) | 衣分 Fibre optic tube yield(%) | 单位面积铃数 Number of bolls/667m2 | 籽棉产量 Seed cotton yield(kg) |
---|---|---|---|---|---|
T1 | 10 700a | 5.36a | 44.2a | 81 570a | 437.22b |
T2 | 10 800a | 5.51a | 44.9a | 82 930a | 456.94a |
CK | 10 000b | 5.12b | 43.3b | 74 680b | 382.36c |
表6 不同处理下棉花产量性状变化
Tab.6 Effects of different treatments on cotton yield traits
处理 Treatment | 单位面积株数/株 Number of plants/667m2 | 单铃重 Single boll weight(g) | 衣分 Fibre optic tube yield(%) | 单位面积铃数 Number of bolls/667m2 | 籽棉产量 Seed cotton yield(kg) |
---|---|---|---|---|---|
T1 | 10 700a | 5.36a | 44.2a | 81 570a | 437.22b |
T2 | 10 800a | 5.51a | 44.9a | 82 930a | 456.94a |
CK | 10 000b | 5.12b | 43.3b | 74 680b | 382.36c |
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