棉花根际溶磷菌WJP-7发酵培养基优化及防病增产效果

doi:10.6048/j.issn.1001-4330.2023.05.026

新疆农业科学 ›› 2023, Vol. 60 ›› Issue (5): 1263-1270.DOI: 10.6048/j.issn.1001-4330.2023.05.026

棉花根际溶磷菌WJP-7发酵培养基优化及防病增产效果

王宁¹^,²(), 史应武¹^,², 牛新湘²^,³, 杨红梅¹^,², 楚敏¹^,², 詹发强¹, 包慧芳¹, 杨蓉¹, 龙宣杞¹, 丁荣荣⁴()

1.新疆农业科学院微生物应用研究所/新疆特殊环境微生物实验室,乌鲁木齐 830091
2.农业农村部西北绿洲农业环境重点实验室,乌鲁木齐 830091
3.新疆农业科学院土壤肥料与农业节水研究所,乌鲁木齐 830091
4.新疆生产建设兵团第七师农业科学研究所,新疆奎屯 833200

收稿日期:2022-08-28 出版日期:2023-05-20 发布日期:2023-05-22
通信作者: 丁荣荣(1978-),女,新疆呼图壁人,助理研究员,研究方向为作物病虫害防治, (E-mail) 462726314@qq.com
作者简介:王宁(1979-),女,山东人,副研究员,研究方向为微生物发酵, (E-mail) 150700126@qq.com
基金资助:
新疆生产建设兵团第七师胡杨河市财政科技计划项目(2020C14);国家自然科学基金地区科学基金项目(31860024);新疆农业科学院青年科技骨干创新能力培养项目(xjnkq-2019014);新疆维吾尔自治区科技计划重大专项(2020A01002);自治区重点研发项目(2021B02004)

Inoculation technology and field application of cotton rhizosphere phosphorus-solubilizing P.Taiwanensis WJP-7

WANG Ning¹^,²(), SHI Yingwu¹^,², NIU Xinxiang²^,³, YANG Hongmei¹^,², CHU Min¹^,², ZHAN Faqiang¹, BAO Huifang¹, YANG Rong¹, LONG Xuanqi¹, DING Rongrong⁴()

1. Institute of Institute of Applied Microbiology, Xinjiang Academy of Agricultural Sciences/Xinjiang Special Environmental Microbiology Laboratory, Urumqi 830091, China
2. Key Laboratory of Northwest Oasis Agricultural Environment, Ministry of Agriculture and Rural Affairs, Urumqi 830091, China
3. Institute of Soil and Fertilizer and Agricultural Water Conservation, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China
4. Seventh Division Agricultural Research Institute, Xinjiang Production and Construction Corps, Kuitun Xinjiang 832000, China

Received:2022-08-28 Published:2023-05-20 Online:2023-05-22
Supported by:
Huyanghe City Financial Science and Technology Project of the 7th Division of Xinjiang Production and Construction Corps(2020C14);National Natural Science Foundation of China(31860024);Innovation Ability Training Project for Young Sci-Tech Backbone Talents Sponsored by Xinjiang Academy of Agricultural Sciences(xjnkq-2019014);Major Science Project of Xinjiang Uygur Autonomous Region(2020A01002);Key R & D Project of Xinjiang Uygur Autonomous Region(2021B02004)

摘要/Abstract

摘要：

【目的】研究棉花根际溶磷菌WJP-7发酵培养基优化及防病增产效果。【方法】采用实验室筛选的高效溶磷菌株P.taiwanensis WJP-7为出发菌株,在优化的假单胞菌发酵培养基上增殖培养。【结果】利用正交试验优化摇瓶发酵培养基,培养基配方为蛋白胨 20 g/L,MgCl₂ 15 g/L,K₂SO₄ 1.4 g/L。使活菌数达到3.6×10⁹CFU/mL。摇瓶增菌发酵最优培养基发酵验证试验结果最高活菌数为3.60×10⁹CFU/mL。菌株WJP-7水剂处理对花期黄萎病防治效果为44.57%,絮期防治效果为50.53%。施用WJP-7水剂对棉田土壤除全氮含量增加不显著,对其它养分和pH值均有显著影响(P<0.05)。菌剂增产率为14.35%和19.51%,【结论】优化了台湾假单胞菌WJP-7摇瓶发酵培养基,菌株WJP-7水剂处理对棉花黄萎病防治效果为50.53%,对棉田土壤除全氮含量不显著外,对其它养分和pH值均显著影响(P<0.05),具有明显的增产效果。

关键词: 棉花; 溶磷菌; 增菌工艺; 田间应用

Abstract:

【Objective】 Inoculation technology and field application of cotton rhizosphere phosphorus-solubilizing P.Taiwanensis WJP-7.【Method】 In this paper, the self-selected high-efficiency phosphorus-dissolving strain P.taiwanensis WJP-7 was used as the starting strain, and it was propagated and cultured in the optimized Pseudomonas fermentation medium.【Results】 The results showed that the shake flask fermentation medium was optimized by orthogonal experiment, and the medium formula was determined as: peptone 20 g/L, magnesium chloride 15 g/L, potassium sulfate 1.4 g/L.The number of viable cells reached 3.6×10⁹CFU/mL.The maximum viable bacteria count of the optimal medium for enrichment fermentation in shake flask was 3.60×10⁹CFU/mL.The control effect of strain WJP-7 water treatment on Verticillium wilt at flowering stage was 44.57%, and the control effect at flocculation stage was 50.53%.The application of microbial inoculants on cotton field soils had no significant increase in total nitrogen content (P>0.05), but significant effects on other nutrients and pH values (P<0.05).The yield increase rates were 14.35% and 19.51%.【Conclusion】 The shake flask fermentation medium of Pseudomonas Taiwan WJP-7 was optimized by orthogonal experiment.Except for the total nitrogen content in cotton field soil, it has a significant effect on other nutrients and pH value (P<0.05), with a significant yield increase effect.

Key words: cotton; phosphorus-dissolving bacteria; enrichment technology; field application.

中图分类号:

S512
S188

王宁, 史应武, 牛新湘, 杨红梅, 楚敏, 詹发强, 包慧芳, 杨蓉, 龙宣杞, 丁荣荣. 棉花根际溶磷菌WJP-7发酵培养基优化及防病增产效果[J]. 新疆农业科学, 2023, 60(5): 1263-1270.

WANG Ning, SHI Yingwu, NIU Xinxiang, YANG Hongmei, CHU Min, ZHAN Faqiang, BAO Huifang, YANG Rong, LONG Xuanqi, DING Rongrong. Inoculation technology and field application of cotton rhizosphere phosphorus-solubilizing P.Taiwanensis WJP-7[J]. Xinjiang Agricultural Sciences, 2023, 60(5): 1263-1270.

图/表 8

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因素 Factor	A蛋白胨 A Peptone (g/L)	B氯化镁 B Mgcl₂ (g/L)	C硫酸钾 C K₂SO₄ (g/L)
Level 1	5	5	0.7
Level 2	10	10	1.4
Level 3	20	15	2.1

因素 Factor	A蛋白胨 A Peptone (g/L)	B氯化镁 B Mgcl₂ (g/L)	C硫酸钾 C K₂SO₄ (g/L)
Level 1	5	5	0.7
Level 2	10	10	1.4
Level 3	20	15	2.1

试验号 Test number	A	B	C	OD₆₀₀
1	1	1	1	0.53
2	1	2	2	0.60
3	1	3	3	0.58
4	2	1	2	1.23
5	2	2	3	1.30
6	2	3	1	1.34
7	3	1	3	1.58
8	3	2	1	1.70
9	3	3	2	1.83
K₁	0.57	1.11	1.19
K₂	1.29	1.20	1.22
K₃	1.70	1.25	1.15
R	1.14	0.14	0.07

试验号 Test number	A	B	C	OD₆₀₀
1	1	1	1	0.53
2	1	2	2	0.60
3	1	3	3	0.58
4	2	1	2	1.23
5	2	2	3	1.30
6	2	3	1	1.34
7	3	1	3	1.58
8	3	2	1	1.70
9	3	3	2	1.83
K₁	0.57	1.11	1.19
K₂	1.29	1.20	1.22
K₃	1.70	1.25	1.15
R	1.14	0.14	0.07

因素 Factor	III型平方和 Type III of sum squares	自由度 DOF	均方 Mean square	F	P
蛋白胨 Peptone	1.974	2	0.987	420.929	0.002
氯化镁 Magnesium chloride	0.029	2	0.014	6.118	0.140
硫酸钾 Potassium sulfate	0.007	2	0.003	1.427	0.412
误差 Error	0.005	2	0.002

棉花根际溶磷菌WJP-7发酵培养基优化及防病增产效果

Inoculation technology and field application of cotton rhizosphere phosphorus-solubilizing P.Taiwanensis WJP-7

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参考文献 29

相关文章 15

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处理 Treatment	病指Disease index		防效Control effect(%)
处理 Treatment	花期 Flowering period	絮期 Boll opening period	花期 Flowering period	絮期 Boll opening period
30×10⁸ CFU/mL WJP-7水剂 30×10⁸CFU/mL WJP-7 Water agent	2.51	3.26	44.57	50.53
65×10⁸ CFU/g芽孢杆菌可湿性粉剂 65×10⁸CFU/g Bacillus wettable powder	1.12	2.45	75.22	62.82
清水Fresh water	4.52	6.59

样品 Specimen	有机质 Organic matter (g/kg)	全氮 Total nitrogen (g/kg)	全磷 Total phos- phorus (g/kg)	全钾 Total potassium (g/kg)	碱解氮 Alkaline hydrolysis nitrogen (mg/kg)	速效磷 Rapidly available phosphorus (mg/kg)	速效钾 Rapidly available potassium (mg/kg)	pH值	总盐 Total salt (g/kg)
T₁	17.54^a	0.89^a	1.19^a	20.70^a	72.24^b	40.93^a	443.83^a	6.99^a	3.28^b
T₂	17.95^a	0.92^a	1.14^a	20.82^a	75.39^a	35.26^b	445.13^a	7.02^a	3.15^b
CK	14.46^b	0.82^a	0.91^b	20.03^c	63.12^b	28.15^c	404.59^b	6.70^b	6.80^a

处理 Treatment	单位面积株数/株 Number of plants/667m²	单铃重 Single boll weight(g)	衣分 Fibre optic tube yield(%)	单位面积铃数 Number of bolls/667m²	籽棉产量 Seed cotton yield(kg)
T₁	10 700^a	5.36^a	44.2^a	81 570^a	437.22^b
T₂	10 800^a	5.51^a	44.9^a	82 930^a	456.94^a
CK	10 000^b	5.12^b	43.3^b	74 680^b	382.36^c

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