Identification and genetic diversity of wild Pleurotus in Xinjiang

doi:10.6048/j.issn.1001-4330.2025.05.025

Abstract

Abstract:

【Objective】 The objective of the study is to analyze their genetic diversity of wild strains of the Pleurotus in Xinjiang in the hope of providing basic materials and theoretical basis for enriching and developing new varieties.【Methods】 The ITS sequence was used to identify the species and the ISSR markers technology to carry out the genetic diversity analysis on 40 wild oyster mushroom strains.【Results】 The mycelia of 40 strains of wild oyster mushroom in Xinjiang were basically white, and there were no significant differences in colony morphology, but certain differences in mycelia growth and growth speed. By ITS sequence analysis, 40 strains could be divided into 4 groups. 10 ISSR primers amplified a total of 83 clear DNA bands, including 82 polymorphic bands with a polymorphism ratio of 98.80%, and the 40 tested strains were divided into 5 groups at the genetic similarity coefficient of 0.66. In general, the 40 strains of wild oyster mushroom tested showed significant genetic diversity, which had important reference value for the breeding of Xinjiang oyster mushroom. 【Conclusion】 Pleurotus spp. strains have the occurrence of genetic differentiation with relatively rich genetic diversity.

Key words: oyster mushroom; Pleurotus ostreatus species complex; ITS; ISSR; genetic diversity

摘要：

【目的】分析新疆野生平菇的遗传多样性,为丰富和开发平菇新品种提供基础材料和理论依据。【方法】采用生物学和ITS、ISSR分子标记技术相结合的方法,研究40个新疆野生平菇菌株进行遗传多样性。【结果】40个新疆野生平菇菌株菌丝基本呈白色,菌落形态差异不显著,菌丝长势和长速方面有一定的区别。经ITS序列分析,可大致将40个菌株划分为4个类群;10条引物共扩增出83条清晰的DNA条带,多态性条带82条,多态性比率为98.80%。遗传相似系数为0.66时,可将40个样本分为5个类群,并显示出较强的地域相关性,供试的40株野生平菇菌株具有显著的遗传多样性。【结论】野生平菇菌株具有丰富的遗传多样性。

关键词: 平菇, 糙皮侧耳复合群, ITS, ISSR, 遗传多样性

CLC Number:

S188

LUO Ying, Nurziya Yarmamat, JIA Wenjie, ZHU Qi, LI Yutong, SHI Wenting, JIA Peisong. Identification and genetic diversity of wild Pleurotus in Xinjiang[J]. Xinjiang Agricultural Sciences, 2025, 62(5): 1266-1272.

罗影, 努尔孜亚·亚力买买提, 贾文捷, 朱琦, 石文婷, 李雨桐, 贾培松. 新疆野生平菇的遗传多样性分析[J]. 新疆农业科学, 2025, 62(5): 1266-1272.

Figures/Tables 7

References 21

[1]	黄年来, 林志彬, 陈国良, 等. 中国食药用菌学[M]. 上海: 上海科学技术文献出版社, 2010.
	HUANG Nianlai, LIN Zhibin, CHEN Guoliang. Medicinal and edible fungi[M]. Shanghai: Shanghai Scientific and Technological Literature Publishing House, 2010.
[2]	边银丙. 食用菌遗传育种学[M]. 北京: 高等教育出版社, 2022.
	BIAN Yinbing. Genetics and breeding of edible mushroom[M]. Beijing: Higher Education Press, 2022.
[3]	张瑞颖, 胡丹丹, 左雪梅, 等. 平菇和双孢蘑菇细菌性褐斑病研究进展[J]. 植物保护学报, 2007, 34(5): 549-554.
	ZHANG Ruiying, HU Dandan, ZUO Xuemei, et al. Research advancement on brown blotch disease of oyster mushroom and button mushroom[J]. Journal of Plant Protection, 2007, 34(5): 549-554.
[4]	李静. 糙皮侧耳物种复合群的物种识别、生物地理及种质资源研究[D]. 昆明: 云南大学, 2019.
	LI Jing. Study on species identification, biogeography and germplasm resources of Pleurotus ostreatus species complex[D]. Kunming: Yunnan University, 2019.
[5]	Li J, Han L H, Liu X B, et al. The saprotrophic Pleurotus ostreatus species complex: late Eocene origin in East Asia, multiple dispersal, and complex speciation[J]. IMA Fungus, 2020, 11: 10.
[6]	中国食用菌协会. 2021年度全国食用菌统计调查结果分析[J]. 中国食用菌, 2023, 42(1): 118-127.
	China Edible Fungi Association. Analysis on the results of national edible fungi statistical survey in 2021[J]. Edible Fungi of China, 2023, 42(1): 118-127.
[7]	Schoch C L, Seifert K A, Huhndorf S, et al. Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi[J]. Proceedings of the National Academy of Sciences of the United States of America, 2012, 109(16): 6241-6246. DOI PMID
[8]	He X L, Li Q, Peng W H, et al. Intra- and inter-isolate variation of ribosomal and protein-coding genes in Pleurotus: implications for molecular identification and phylogeny on fungal groups[J]. BMC Microbiology, 2017, 17(1): 139.
[9]	Ekowati N, Mumpuni A, Muljowati J S, et al. Genetic diversity of Pleurotus ostreatus (Jacq.) P. Kumm. strains in Java based on random amplified polymorphic DNA markers[J]. Biodiversitas Journal of Biological Diversity, 2021, 22(6): 3488-3493.
[10]	Lin P, Yan Z F, Kook M, et al. Genetic and chemical diversity of edible mushroom Pleurotus species[J]. BioMed Research International, 2022, 2022: 6068185.
[11]	努尔孜亚·亚力买买提, 贾文捷, 郝敬喆, 等. 野生平菇菌株的培养特征及遗传多样性分析[J]. 新疆农业科学, 2021, 58(1): 143-150. DOI
	NuerziyaYalimaimaiti, JIA Wenjie, HAO Jingzhe, et al. Analysis of culture characteristics and genetic diversity of wild Pleurotus ostreatus strains[J]. Xinjiang Agricultural Sciences, 2021, 58(1): 143-150. DOI
[12]	Barh A, Sharma V P, Annepu S K, et al. Estimation of genetic diversity for interspecific hybridization in Pleurotus spp[J]. Vegetos, 2024, 37(1): 155-164.
[13]	Li J, He X, Liu X B, et al. Species clarification of oyster mushrooms in China and their DNA barcoding[J]. Mycological Progress, 2017, 16(3): 191-203.
[14]	Flores G A, Girometta C E, Cusumano G, et al. Diversity of Pleurotus spp. (Agaricomycetes) and their metabolites of nutraceutical and therapeutic importance[J]. International Journal of Medicinal Mushrooms, 2023, 25(6): 1-20.
[15]	Wei Y M, Li L, Liu Y, et al. Identification techniques and detection methods of edible fungi species[J]. Food Chemistry, 2022, 374: 131803.
[16]	Yan Z Y, Liu H G, Li J Q, et al. Application of identification and evaluation techniques for edible mushrooms: a review[J]. Critical Reviews in Analytical Chemistry, 2023, 53(3): 634-654.
[17]	NY/T 1730—2009. 食用菌菌种真实性鉴定 ISSR法[S].
	NY/T 1730—2009. Verification of genuineness for edible mushroom spawn--ISSR[S].
[18]	Gardes M, Bruns T D. ITS primers with enhanced specificity for basidiomycetes—application to the identification of mycorrhizae and rusts[J]. Molecular Ecology, 1993, 2(2): 113-118. DOI PMID
[19]	朱坚. 食用菌品种特性与栽培[M]. 福州: 福建科学技术出版社, 2011.
	ZHU Jian. Characteristics and cultivation of edible fungi varieties[M]. Fuzhou: Fujian Science & Technology Publishing House, 2011.
[20]	李辉平. ISSR在食用菌遗传多样性研究中的应用[D]. 北京: 中国农业科学院, 2007.
	LI Huiping. Application of ISSR in genetic diversity analysis for mushroom cultivars[D]. Beijing: Chinese Academy of Agricultural Sciences, 2007.
[21]	郑素月, 张金霞, 王贺祥, 等. 我国栽培平菇近缘种的多相分类[J]. 中国食用菌, 2003, 22(3): 3-6.
	ZHENG Suyue, ZHANG Jinxia, WANG Hexiang, et al. Polyphasic taxonomy of cultivated oyster mushrooms in China[J]. Edible Fungi of China, 2003(3): 3-6.

序号 No.	引物编号 Primer No.	引物序列 Primer sequence (5'-3')	退火温度 Annealing temper- ature(℃)
1	P₁	TGCACACACACACAC	53
2	P₂	GTGACACACACACAC	48
3	P₁₀	GAGAGAGAGAGAGAGAC	52
4	P₁₁	GAGAGAGAGAGAGAGAAC	55
5	P₁₈	ACACACACACACACACC	53
6	P₁₉	ACACACACACACACACCT	53
7	P₂₂	AAGAAGAAGAAGAAGAAGC	53
8	P₂₄	CACGAGAGAGAGAGAGA	50
9	P₂₅	GAGAGAGAGAGAGAGACC	55
10	P₂₆	CACCACACACACACACA	55

序号 No.	引物编号 Primer No.	引物序列 Primer sequence (5'-3')	退火温度 Annealing temper- ature(℃)
1	P₁	TGCACACACACACAC	53
2	P₂	GTGACACACACACAC	48
3	P₁₀	GAGAGAGAGAGAGAGAC	52
4	P₁₁	GAGAGAGAGAGAGAGAAC	55
5	P₁₈	ACACACACACACACACC	53
6	P₁₉	ACACACACACACACACCT	53
7	P₂₂	AAGAAGAAGAAGAAGAAGC	53
8	P₂₄	CACGAGAGAGAGAGAGA	50
9	P₂₅	GAGAGAGAGAGAGAGACC	55
10	P₂₆	CACCACACACACACACA	55

序号 No	菌株编号 Strain	采集地 Sources	菌落形态 Colony morphology	菌丝生长速度 Growth vigor (mm/d)	菌丝长势 Growth potentia
1	XPG001	哈巴河县	菌丝浓白,边缘整齐	11.78±0.64^aAB	++++
2	XPG002	哈巴河县	菌丝较浓密,边缘整齐	6.15±0.30	+++
3	XPG003	哈巴河县	菌丝浓密,边缘整齐	11.66±0.32^abABC	++++
4	XPG004	哈巴河县	气生菌丝发达,边缘整齐	11.20±0.00^bcdABCDE	+++
5	XPG006	哈巴河县	菌丝较浓密,边缘整齐	5.75±1.96	++
6	XPG007	青河县	菌丝浓密,边缘整齐	11.51±1.78^abcABC	++++
7	XPG009	哈巴河县	菌丝较浓密,边缘较整齐	10.23±2.11^ghiHI	++++
8	XPG010	青河县	气生菌丝发达,边缘整齐	10.17±2.34^ghiHI	++++
9	XPG011	青河县	菌丝浓白,边缘整齐	10.75±2.46^defEFGH	++++
10	XPG012	青河县	菌丝较浓密,边缘整齐	6.12±2.11	++
11	XPG013	青河县	菌丝浓白,边缘整齐	11.51±1.30^abcABC	+++
12	XPG014	察布查尔锡铂自治县	菌丝,较浓密气生菌丝发达	10.75±2.46^defEFGH	+++
13	XPG015	察布查尔锡铂自治县	菌丝白色较浓密边缘整齐	6.50±3.54	+++
14	XPG016	察布查尔锡铂自治县	气生菌丝发达,边缘整齐	11.57±0.67^abcABC	+++
15	XPG017	察布查尔锡铂自治县	气生菌丝发达,边缘整齐	11.85±0.25^aA	++++
16	XPG018	察布查尔锡铂自治县	气生菌丝发达,边缘不整齐	10.50±1.42^fgFGHIJ	+++
17	XPG019	额敏县	菌丝浓白,边缘整齐	10.43±1.26^fghGHIJ	++++
18	XPG021	额敏县	菌丝浓白,边缘整齐	11.85±0.34^aA	++++
19	XPG022	额敏县	菌丝浓白,边缘整齐	9.11±0.34	+++
20	XPG023	额敏县	气生菌丝发达,边缘整齐	10.77±1.94^defDEFGH	++++
21	XPG024	额敏县	气生菌丝发达,边缘整齐	11.07±1.82^cdeCDEFG	++++
22	XPG025	哈巴河县	气生菌丝发达,边缘不整齐	6.92±1.17	++
23	XPG026	哈巴河县	菌丝较浓白,边缘较整齐	9.58±1.93	+++
24	XPG027	哈巴河县	菌丝较浓白, 边缘整齐	9.91±1.21	++++
25	XPG028	哈巴河县	菌丝浓白,边缘整齐	10.41±0.53^fghGHIJ	+++
26	XPG029	吉木萨尔县	气生菌丝发达,边缘整齐	10.56±1.77^fgEFGHIJ	++++
27	XPG030	吉木萨尔县	菌丝浓密,边缘整齐	11.15±0.71^bcdBCDEF	++++
28	XPG031	吉木萨尔县	菌丝浓密,边缘整齐	11.60±1.04^abABC	++++
29	XPG032	吉木萨尔县	菌丝较浓密,边缘较整齐	9.96±0.08^hi	+++
30	XPG033	吉木萨尔县	气生菌丝发达,边缘整齐	10.40±0.66^fghiGHIJ	++++
31	XPG034	乌鲁木齐市	菌丝较浓密边缘较整齐	11.63±0.26^abABC	++++
32	XPG035	乌鲁木齐市	菌丝浓密,边缘整齐	11.77±0.35^aAB	++++
33	XPG036	乌鲁木齐市	菌丝浓密边缘较整齐	11.62±0.80^abABC	++++
34	XPG037	乌鲁木齐市	菌丝较稀疏,边缘较整齐	11.45±0.82^abcABC	+++
35	XPG038	吉木萨尔县	菌丝较浓密,边缘整齐	10.17±2.74^ghiHI	++++
36	XPG039	吉木萨尔县	菌丝浓密,边缘较整齐	8.96±1.56	++++
37	XPG040	吉木萨尔县	气生菌丝发达,边缘整齐	11.40±1.45^abcABCD	+++
38	XPG043	哈巴河县	菌丝较浓密,边缘不整齐	10.12±2.21^ghiHI	+++
39	XPG047	哈巴河县	气生菌丝发达,边缘整齐	10.59±3.37^efgEFGHI	++++
40	XPG055	乌鲁木齐市	菌丝浓密,边缘整齐	11.62±0.94abABC	++++

序号 No	菌株编号 Strain	采集地 Sources	菌落形态 Colony morphology	菌丝生长速度 Growth vigor (mm/d)	菌丝长势 Growth potentia
1	XPG001	哈巴河县	菌丝浓白,边缘整齐	11.78±0.64^aAB	++++
2	XPG002	哈巴河县	菌丝较浓密,边缘整齐	6.15±0.30	+++
3	XPG003	哈巴河县	菌丝浓密,边缘整齐	11.66±0.32^abABC	++++
4	XPG004	哈巴河县	气生菌丝发达,边缘整齐	11.20±0.00^bcdABCDE	+++
5	XPG006	哈巴河县	菌丝较浓密,边缘整齐	5.75±1.96	++
6	XPG007	青河县	菌丝浓密,边缘整齐	11.51±1.78^abcABC	++++
7	XPG009	哈巴河县	菌丝较浓密,边缘较整齐	10.23±2.11^ghiHI	++++
8	XPG010	青河县	气生菌丝发达,边缘整齐	10.17±2.34^ghiHI	++++
9	XPG011	青河县	菌丝浓白,边缘整齐	10.75±2.46^defEFGH	++++
10	XPG012	青河县	菌丝较浓密,边缘整齐	6.12±2.11	++
11	XPG013	青河县	菌丝浓白,边缘整齐	11.51±1.30^abcABC	+++
12	XPG014	察布查尔锡铂自治县	菌丝,较浓密气生菌丝发达	10.75±2.46^defEFGH	+++
13	XPG015	察布查尔锡铂自治县	菌丝白色较浓密边缘整齐	6.50±3.54	+++
14	XPG016	察布查尔锡铂自治县	气生菌丝发达,边缘整齐	11.57±0.67^abcABC	+++
15	XPG017	察布查尔锡铂自治县	气生菌丝发达,边缘整齐	11.85±0.25^aA	++++
16	XPG018	察布查尔锡铂自治县	气生菌丝发达,边缘不整齐	10.50±1.42^fgFGHIJ	+++
17	XPG019	额敏县	菌丝浓白,边缘整齐	10.43±1.26^fghGHIJ	++++
18	XPG021	额敏县	菌丝浓白,边缘整齐	11.85±0.34^aA	++++
19	XPG022	额敏县	菌丝浓白,边缘整齐	9.11±0.34	+++
20	XPG023	额敏县	气生菌丝发达,边缘整齐	10.77±1.94^defDEFGH	++++
21	XPG024	额敏县	气生菌丝发达,边缘整齐	11.07±1.82^cdeCDEFG	++++
22	XPG025	哈巴河县	气生菌丝发达,边缘不整齐	6.92±1.17	++
23	XPG026	哈巴河县	菌丝较浓白,边缘较整齐	9.58±1.93	+++
24	XPG027	哈巴河县	菌丝较浓白, 边缘整齐	9.91±1.21	++++
25	XPG028	哈巴河县	菌丝浓白,边缘整齐	10.41±0.53^fghGHIJ	+++
26	XPG029	吉木萨尔县	气生菌丝发达,边缘整齐	10.56±1.77^fgEFGHIJ	++++
27	XPG030	吉木萨尔县	菌丝浓密,边缘整齐	11.15±0.71^bcdBCDEF	++++
28	XPG031	吉木萨尔县	菌丝浓密,边缘整齐	11.60±1.04^abABC	++++
29	XPG032	吉木萨尔县	菌丝较浓密,边缘较整齐	9.96±0.08^hi	+++
30	XPG033	吉木萨尔县	气生菌丝发达,边缘整齐	10.40±0.66^fghiGHIJ	++++
31	XPG034	乌鲁木齐市	菌丝较浓密边缘较整齐	11.63±0.26^abABC	++++
32	XPG035	乌鲁木齐市	菌丝浓密,边缘整齐	11.77±0.35^aAB	++++
33	XPG036	乌鲁木齐市	菌丝浓密边缘较整齐	11.62±0.80^abABC	++++
34	XPG037	乌鲁木齐市	菌丝较稀疏,边缘较整齐	11.45±0.82^abcABC	+++
35	XPG038	吉木萨尔县	菌丝较浓密,边缘整齐	10.17±2.74^ghiHI	++++
36	XPG039	吉木萨尔县	菌丝浓密,边缘较整齐	8.96±1.56	++++
37	XPG040	吉木萨尔县	气生菌丝发达,边缘整齐	11.40±1.45^abcABCD	+++
38	XPG043	哈巴河县	菌丝较浓密,边缘不整齐	10.12±2.21^ghiHI	+++
39	XPG047	哈巴河县	气生菌丝发达,边缘整齐	10.59±3.37^efgEFGHI	++++
40	XPG055	乌鲁木齐市	菌丝浓密,边缘整齐	11.62±0.94abABC	++++

序号 No.	引物 Primer	扩增条带数 Total bands(n)	多态性条带数 Polymorphic bands(k)	多态性条带比率 Percentage of polymorphic bands(P/% )
1	P₁	6	6	100.00
2	P₂	7	6	85.71
3	P₁₀	8	8	100.00
4	P₁₁	8	8	100.00
5	P₁₈	8	8	100.00
6	P₁₉	9	9	100.00
7	P₂₂	11	11	100.00
8	P₂₄	7	7	100.00
9	P₂₅	9	9	100.00
10	P₂₆	10	10	100.00
总计(Total)		83	82
平均 (Average)		8.3	8.2	98.80