Abstract:

【Objective】 This study aims to investigate the genetic effects of lambing traits COIL and BMPR-IB genes in the offspring of Duolang sheep and Hu sheep crossbreeding (Duohu sheep crossbreeding) cascade crosses by analyzing the association between COIL and BMPR-IB genes and the number of lambs produced. 【Methods】 Randomly selected F₁ (228), F₂ (254), and F₃ (252) generations of Duohu crossbred sheep were subjected to whole blood DNA extraction, and the COIL gene was genotyped using amplification refractory mutation system-PCR (ARMS-PCR) technique, and the BMPR-IB gene was analyzed in association with the number of lambs produced after genotyping using PCR-restriction fragment length polymorphism (PCR-RFLP) technique. 【Results】 In the Duohu F₁ sheep, the lambing numbers of the mutant pure GG type were significantly higher than those of the mutant heterozygous CG and the wild CC type (P<0.01), and that of the mutant heterozygous CG type were significantly higher than that of the wild CC type (P<0.01); in the Duohu F₂ sheep, the differences between the number of lambs produced by the mutant pure GG type and the mutant heterozygous CG and wild CC types were all highly significant (P<0.01); in the Duohu F₃ sheep, the number of lambs produced by the mutant pure GG and the mutant heterozygous CG types was highly significantly higher than that of the CC type (P<0.01), and the number of lambs produced by the GG type was highly significantly higher than that of the CG type (P<0.05). In the Duohu F₁, F₂ and F₃ sheep, the numbers of lambs produced by the mutant pure BB type were highly significantly higher than the number of lambs produced by the mutant heterozygous B+ type and the wild++ type (P<0.01), and the number of lambs produced by the mutant heterozygous B+ type was highly significantly higher than that of the wild++ type (P<0.01). 【Conclusion】 Both COIL gene and BMPR-IB gene have significant effect on lambing number of Duo and Hu crossbred sheep. However, the BMPR-IB gene has a low mutation rate in the progeny of Duohu sheep, which may not be suitable as a key gene for screening the double lambing population of Duohu sheep, and the COIL gene can be a new candidate gene for screening the high reproduction rate of Duohu sheep.

Key words: Duolang sheep; Hu sheep; crossbreed; lambing traits; COIL gene; BMPR-IB gene; genetic effects

摘要：

【目的】 分析COIL及BMPR-IB基因与产羔数关联性,探究多浪羊与湖羊(简称多湖杂交)后代产羔性状COIL及BMPR-IB基因的遗传效应。【方法】 研究以多浪羊与湖羊级进杂交F₁代、F₂代、F₃代为研究对象,随机选取多湖杂交F₁代(228只)、F₂代(254只)、F₃代(252只),进行全血DNA提取,COIL基因利用扩增阻滞突变系统PCR(ARMS-PCR)技术基因分型、BMPR-IB基因利用限制性内切酶片段长度多态性技术(PCR-RFLP)基因分型后,与产羔数关联分析。【结果】 在多湖杂交F₁代中,突变纯合GG型产羔数极显著高于突变杂合CG、野生CC型产羔数(P<0.01),突变杂合CG型产羔数极显著高于野生CC型产羔数(P<0.01);在多湖杂交F₂代中突变纯合GG型与突变杂合CG、野生CC型产羔数间均差异极显著(P<0.01);在多湖杂交F₃代中,突变纯合GG、突变杂合CG型产羔数极显著高于CC型(P<0.01),GG型产羔数极显著高于CG型(P<0.05)。在多湖杂交F₁、F₂、F₃代中均为突变纯合BB型产羔数极显著高于突变杂合B+型、野生++型产羔数(P<0.01),突变杂合B+型产羔数极显著高于野生++型产羔数(P<0.01)。【结论】 COIL基因与BMPR-IB基因均对多湖杂交后代产羔数影响显著,但BMPR-IB基因在多湖杂交后代突变率较低,可能不适合作为筛选多湖杂交双羔群体的关键基因,COIL基因可以作为筛选多湖杂交高繁殖率的新候选基因。

关键词: 多浪羊, 湖羊, 杂交, 产羔性状, COIL基因, BMPR-IB基因, 遗传效应