Anther bacterial diversity of Korla Fragrant Pear before and after flowering

doi:10.6048/j.issn.1001-4330.2024.08.018

Abstract

Abstract:

【Objective】 The study investigated the bacterial presence in the anthers of Korla Fragrant pear blossoms, analyzing the changes in bacterial populations before and after flowering, to provide microbial resources for the prevention and control of common diseases in Fragrant pear trees in southern Xinjiang.【Methods】 The flat-plate coating method was employed to isolate and purify the bacteria from the anthers of Fragrant pear blossoms before and after flowering. The bacterial diversity was analyzed, and molecular identification was conducted using 16S rDNA gene sequencing to determine their taxonomic status.【Results】 The bacterial species carried by the anthers of korla Fragrant pear blossoms before and after flowering exhibited diversity. Nine strains were randomly selected for molecular identification from the bacteria isolated from the anthers before flowering, all of which were categorized under the Bacillus genus, including Bacillus subtilis, Bacillus amyloliquefaciens, and Bacillus velezensis. Additionally, seven strains were randomly selected from the bacteria isolated after flowering; these included not only Bacillus genus but also bacteria from the Sphingobium genus, indicating greater diversity in the bacteria after flowering compared to before.【Conclusion】 Among the bacteria carried by the anthers before flowering, five strains were identified as Bacillus subtilis, two as Bacillus amyloliquefaciens, and two as Bacillus velezensis. After flowering, the bacteria included three strains of Bacillus subtilis, two strains of Bacillus amyloliquefaciens, one strain of Bacillus paranthracis, and one strain of Sphingobacterium multivorum.

Key words: Korla Fragrant pear anther; Bacteria; Diversity

摘要：

【目的】研究库尔勒香梨花药细菌的多样性,分析库尔勒香梨开花前后花药细菌变化,为新疆南疆梨树常见病害的防治提供菌种资源。【方法】采用平板涂布法分离和纯化香梨开花前后花药细菌,分析细菌多样性,并借助16S rDNA基因序列分析技术对其进行分子鉴定,确定其分类地位。【结果】香梨开花前后花药所携带的细菌种类具有多样性。对开花前后花药分离的菌株分别随机挑选9株和7株进行分子鉴定,筛选的9株开花前花药细菌全部归属于芽孢杆菌属,其中包括枯草芽孢杆菌、解淀粉芽孢杆菌和贝莱斯芽孢杆菌。筛选的7株开花后花药细菌除了有芽孢杆菌属细菌,也包括鞘氨醇杆菌属细菌,开花后花药细菌多样性大于开花前。【结论】开花前花药所携带的细菌其中有5株为枯草芽孢杆菌,2株为解淀粉芽孢杆菌,2株为贝莱斯芽孢杆菌。开花后花药所携带的细菌其中有3株为枯草芽孢杆菌,2株为解淀粉芽孢杆菌,1株为副炭疽芽孢杆菌,1株为多食鞘氨醇杆菌。

关键词: 库尔勒香梨花药, 细菌, 多样性

CLC Number:

S661.2

WANG Qingpeng, YAN Chengcai, WANG Zhe, GOU Changqing, WANG Lan, FENG Hongzu, HAO Haiting. Anther bacterial diversity of Korla Fragrant Pear before and after flowering[J]. Xinjiang Agricultural Sciences, 2024, 61(8): 1976-1982.

王庆朋, 闫成才, 王喆, 苟长青, 王兰, 冯宏祖, 郝海婷. 库尔勒香梨开花前后花药细菌多样性分析[J]. 新疆农业科学, 2024, 61(8): 1976-1982.

Figures/Tables 10

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采样批次 Sampling batch	采样地点 Sampling site	采样时间 Sampling time (M/D)	采样经度纬度 Sampling latitude and longitude
Ⅰ	风雨操场旁采样	4/6	81°17'18″ E 40°32'30″ N
Ⅱ	工科楼旁采样	4/11	81°17'52″ E 40°32'30″ N
Ⅲ	校园外采样	4/11	81°17'52″ E 40°32'30″ N

采样批次 Sampling batch	采样地点 Sampling site	采样时间 Sampling time (M/D)	采样经度纬度 Sampling latitude and longitude
Ⅰ	风雨操场旁采样	4/6	81°17'18″ E 40°32'30″ N
Ⅱ	工科楼旁采样	4/11	81°17'52″ E 40°32'30″ N
Ⅲ	校园外采样	4/11	81°17'52″ E 40°32'30″ N

采样批次 Sampling batch	采样地点 Sampling site	采样时间 Sampling time (M/D)	采样经度纬度 Sampling latitude and longitude
①	工科楼旁采样	4/9	81°17'52″ E 40°32'30″ N
②	青少宫旁采样	4/13	81°17'19″ E 40°32'49″ N
③	校园外采样	4/18	81°15'42″ E 40°33'49″ N
④	工科楼旁采样	4/20	81°17'52″ E 40°32'30″ N
⑤	风雨操场旁采样	5/4	81°17'18″ E 40°32'30″ N

采样批次 Sampling batch	采样地点 Sampling site	采样时间 Sampling time (M/D)	采样经度纬度 Sampling latitude and longitude
①	工科楼旁采样	4/9	81°17'52″ E 40°32'30″ N
②	青少宫旁采样	4/13	81°17'19″ E 40°32'49″ N
③	校园外采样	4/18	81°15'42″ E 40°33'49″ N
④	工科楼旁采样	4/20	81°17'52″ E 40°32'30″ N
⑤	风雨操场旁采样	5/4	81°17'18″ E 40°32'30″ N

体系成分 System component	用量 Dosage (μL)
模板 Template	1
27F/1492R引物 Primer 27F/1492R (10 mol/μL)	0.5
Taq Master Mix	12.5
ddH₂O 10.5 μL	10.5