Xinjiang Agricultural Sciences ›› 2024, Vol. 61 ›› Issue (4): 1021-1028.DOI: 10.6048/j.issn.1001-4330.2024.04.027
• Plant Protection·Microbes·Agricultural Equipment Engineering and Mechanization • Previous Articles Next Articles
SUN Jian1(), LI Xue2, CHU Min1, GU Meiying1, Ainijiang Ersiman1, ZHU Jin1, HE Qi2, TAN Huilin3, ZHANG Zhidong1,2(
)
Received:
2023-08-07
Online:
2024-04-20
Published:
2024-05-31
Correspondence author:
ZHANG Zhidong
Supported by:
孙建1(), 李雪2, 楚敏1, 顾美英1, 艾尼江·尔斯满1, 朱静1, 何齐2, 谭慧林3, 张志东1,2(
)
通讯作者:
张志东
作者简介:
孙建(1977-),男,新疆乌鲁木齐人,副研究员,研究方向为特殊环境微生物及益生菌资源,(E-mail)276255465@qq.com
基金资助:
CLC Number:
SUN Jian, LI Xue, CHU Min, GU Meiying, Ainijiang Ersiman, ZHU Jin, HE Qi, TAN Huilin, ZHANG Zhidong. Screening,identification and characteristics of Lactic acid bacteria from raw camel milk[J]. Xinjiang Agricultural Sciences, 2024, 61(4): 1021-1028.
孙建, 李雪, 楚敏, 顾美英, 艾尼江·尔斯满, 朱静, 何齐, 谭慧林, 张志东. 原驼乳中乳酸菌的分离筛选及特性分析[J]. 新疆农业科学, 2024, 61(4): 1021-1028.
菌株 Bacterial strain | 温度 Temperature(℃) | NaCl (%) | pH | 胆盐 Bile salt(%) | 牛奶胨化 Milk peptone | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
20 | 45 | 3 | 5 | 7 | 3 | 4 | 0.5 | 胨化 Peptoniz- ation | 产香 Aroma production | |||||
T6 | w | + | + | + | - | - | w | ++ | + | + | ||||
T7 | w | + | + | + | - | - | w | ++ | + | + | ||||
T12 | + | + | + | + | - | - | w | ++ | + | ++ | ||||
T16 | w | + | + | + | - | - | w | ++ | + | + |
Tab.1 The growth of representative strains under different conditions
菌株 Bacterial strain | 温度 Temperature(℃) | NaCl (%) | pH | 胆盐 Bile salt(%) | 牛奶胨化 Milk peptone | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
20 | 45 | 3 | 5 | 7 | 3 | 4 | 0.5 | 胨化 Peptoniz- ation | 产香 Aroma production | |||||
T6 | w | + | + | + | - | - | w | ++ | + | + | ||||
T7 | w | + | + | + | - | - | w | ++ | + | + | ||||
T12 | + | + | + | + | - | - | w | ++ | + | ++ | ||||
T16 | w | + | + | + | - | - | w | ++ | + | + |
菌株 Bacterial strain | 功能酶Functional enzyme | ||||
---|---|---|---|---|---|
蛋白酶 Protease | 淀粉酶 Amylase | 果胶酶 Pectinase | 酯化酶 Esterase | 纤维素酶 Cellulase | |
T6 | + | - | - | - | - |
T7 | ++ | - | - | - | + |
T12 | - | - | - | - | - |
T16 | + | - | - | - | - |
Tab.2 Functional enzyme activity of strains
菌株 Bacterial strain | 功能酶Functional enzyme | ||||
---|---|---|---|---|---|
蛋白酶 Protease | 淀粉酶 Amylase | 果胶酶 Pectinase | 酯化酶 Esterase | 纤维素酶 Cellulase | |
T6 | + | - | - | - | - |
T7 | ++ | - | - | - | + |
T12 | - | - | - | - | - |
T16 | + | - | - | - | - |
菌株 Bacterial strain | 指示菌Indicator bacteria | |||||
---|---|---|---|---|---|---|
大肠埃希菌 Escherichia coli | 枯草芽孢杆菌 Bacillus subtilis | 金黄色葡萄球菌 Staphylococcus aureus | 扩展青霉 Expanded cyanobacteria | 黑曲霉 Aspergillus niger | 白色念珠菌 Candida albicans | |
T6 | +++ | + | +++ | - | - | - |
T7 | - | - | + | - | - | - |
T12 | ++ | + | +++ | - | - | - |
T16 | +++ | - | ++ | - | - | - |
Tab.3 Inhibition character of tested strains against indicator strains
菌株 Bacterial strain | 指示菌Indicator bacteria | |||||
---|---|---|---|---|---|---|
大肠埃希菌 Escherichia coli | 枯草芽孢杆菌 Bacillus subtilis | 金黄色葡萄球菌 Staphylococcus aureus | 扩展青霉 Expanded cyanobacteria | 黑曲霉 Aspergillus niger | 白色念珠菌 Candida albicans | |
T6 | +++ | + | +++ | - | - | - |
T7 | - | - | + | - | - | - |
T12 | ++ | + | +++ | - | - | - |
T16 | +++ | - | ++ | - | - | - |
抗生素种类 Types of antibi otics | 受试菌株 Test strain | |||
---|---|---|---|---|
T6 | T7 | T12 | T16 | |
青霉素PEN | +++ | + | ++ | +++ |
红霉素ERY | +++ | ++ | + | +++ |
四环素TET | - | - | ++ | +++ |
链霉素STR | - | - | + | + |
庆大霉素GEN | + | + | - | +++ |
卡那霉素KAN | ++ | + | ++ | ++ |
诺氟沙星NOR | - | - | - | - |
氨苄西林AMP | + | - | - | + |
利福平RIF | +++ | ++ | +++ | +++ |
万古霉素VAN | - | - | + | - |
Tab.4 Antibiotic sensitivity of the strains
抗生素种类 Types of antibi otics | 受试菌株 Test strain | |||
---|---|---|---|---|
T6 | T7 | T12 | T16 | |
青霉素PEN | +++ | + | ++ | +++ |
红霉素ERY | +++ | ++ | + | +++ |
四环素TET | - | - | ++ | +++ |
链霉素STR | - | - | + | + |
庆大霉素GEN | + | + | - | +++ |
卡那霉素KAN | ++ | + | ++ | ++ |
诺氟沙星NOR | - | - | - | - |
氨苄西林AMP | + | - | - | + |
利福平RIF | +++ | ++ | +++ | +++ |
万古霉素VAN | - | - | + | - |
菌株 Bacterial strain | 生物胺Biogenic amine | 吲哚 Indole | 溶血性 Hemolysis | 硝基还原酶 Nitroreductase | ||||
---|---|---|---|---|---|---|---|---|
精胺 Spermine | 酪胺 Tyramine | 色胺 Tryptamine | 尸胺 Cadaverine | 组胺 Histamine | ||||
T6 | - | - | - | - | - | - | - | - |
T7 | - | - | - | - | - | - | - | - |
T12 | - | + | + | - | + | + | - | - |
T16 | - | - | - | - | - | - | - | - |
*E.coli | (CK: -) | + | + | + |
Tab.5 The detection results of harmful metabolites of strains
菌株 Bacterial strain | 生物胺Biogenic amine | 吲哚 Indole | 溶血性 Hemolysis | 硝基还原酶 Nitroreductase | ||||
---|---|---|---|---|---|---|---|---|
精胺 Spermine | 酪胺 Tyramine | 色胺 Tryptamine | 尸胺 Cadaverine | 组胺 Histamine | ||||
T6 | - | - | - | - | - | - | - | - |
T7 | - | - | - | - | - | - | - | - |
T12 | - | + | + | - | + | + | - | - |
T16 | - | - | - | - | - | - | - | - |
*E.coli | (CK: -) | + | + | + |
[1] |
Burgain J, Scher J, Francius G, et al. Lactic acid bacteria in dairy food: surface characterization and interactions with food matrix components[J]. Advances in Colloid and Interface Science, 2014, 213: 21-35.
DOI PMID |
[2] | Evivie S E, Huo G C, Igene J O, et al. Some current applications, limitations and future perspectives of lactic acid bacteria as probiotics[J]. Food & Nutrition Research, 2017, 61(1): 1318034. |
[3] | 郝晓丽. 驼乳和牛乳乳清蛋白抗氧化活性的比较分析及其应用研究[D]. 呼和浩特: 内蒙古农业大学, 2020. |
HAO Xiaoli. Comparative analysis and application of antioxidant activity of whey protein from camel milk and bovine milk[D]. Huhhot: Inner Mongolia Agricultural University, 2020. | |
[4] | 张苗苗, 倪永清. 新疆哈密地区原驼乳中乳酸菌多样性分析[J]. 安徽农业科学, 2019, 47(15): 163-166, 182. |
ZHANG Miaomiao, NI Yongqing. Biodiversity of lactic acid bacteria isolated from camel milk of Hami Area in Xinjiang[J]. Journal of Anhui Agricultural Sciences, 2019, 47(15): 163-166, 182. | |
[5] | 张哲, 刘小鸣, 陈卫. 内蒙古传统发酵驼乳中乳酸菌和酵母菌的分离鉴定及其生物多样性分析[J]. 中国食品学报, 2018, 18(7): 230-238. |
ZHANG Zhe, LIU Xiaoming, CHEN Wei. Isolation and identification of lactic acid bacteria and yeast and biodiversity analysis of traditional fermented camel milk in Inner Mongolia[J]. Journal of Chinese Institute of Food Science and Technology, 2018, 18(7): 230-238. | |
[6] | FAHAD Shah, 徐鸿洋, 智超, 等. 阿拉善双峰驼酸驼乳中乳酸菌的分离鉴定及耐酸和耐盐研究[J]. 食品研究与开发, 2020, 41(18): 189-194. |
SHAH F, XU Hongyang, ZHI Chao, et al. Separation, Identification, Acid-Resistent and Halotolerant Characterization of Lactic acid bacteria from Shubat of Alashan Bactrian Camel[J]. Food Research and Development, 2020, 41(18): 189-194. | |
[7] | 拉提帕·艾尔肯. 新疆传统发酵驼乳中发酵菌的分离鉴定及免疫调节作用[D]. 乌鲁木齐: 新疆医科大学, 2013. |
Latipa Aierken. Isolation and identification of zymocyte from the traditional fermentation of camel milk in Xinjiang and immune adjustment effect[D]. Urumqi: Xinjiang Medical University, 2013. | |
[8] | 林杨, 顾美英, 孙建, 等. 阿克苏地区传统酸乳中乳酸菌筛选及安全性初步评价[J]. 微生物学杂志, 2022, 42(1): 26-33. |
LIN Yang, GU Meiying, SUN Jian, et al. Screening and safety evaluation of lactobacteria from traditional yogurt in Aksu Region[J]. Journal of Microbiology, 2022, 42(1): 26-33. | |
[9] | 东秀珠, 蔡妙英. 常见细菌系统鉴定手册[M]. 北京: 科学出版社, 2001: 364-370. |
DONG Xiuzhu, CAI Miaoying. Handbook of identification of common bacterial systems[M]. Beijing: Science Press, 2001: 364-370. | |
[10] | 刘晓静, 楚敏, 朱静, 等. 库车小白杏内生细菌多样性及其产酶特性[J]. 食品科学, 2020, 41(14): 203-209. |
LIU Xiaojing, CHU Min, ZHU Jing, et al. Diversity and functional enzymes of endophytic bacteria in kuqa apricot[J]. Food Science, 2020, 41(14): 203-209.
DOI |
|
[11] | 林杨. 高产γ-氨基丁酸乳酸菌的选育及其发酵工艺优化[D]. 乌鲁木齐: 新疆农业大学, 2021. |
LIN Yang. Breeding of lactic acid bacteria with high yield of γ -aminobutyric acid and optimization of its fermentation technology[D]. Urumqi: Xinjiang Agricultural University, 2021. | |
[12] | 李权威, 张开屏, 赵艳红, 等. 风干羊肉中乳酸菌的体内外抗氧化特性[J]. 微生物学通报, 2020, 47(12): 4094-4104. |
LI Quanwei, ZHANG Kaibing, ZHAO Yanhong, et al. Antioxidation properties in vitro and in vivo of Lactobacillus in air-dried mutton[J]. Microbiology China, 2020, 47(12): 4094-4104. | |
[13] | 冯大伟, 周家春. 益生乳酸菌的纸片扩散法药敏性试验评价[J]. 微生物学通报, 2010, 37(3): 454-464. |
FENG Dawei, ZHOU Jiachun. Evaluation of agar disc diffusion method for determining antibiotic susceptibility of lactic acid bacteria strains by a selected culture medium[J]. Microbiology China, 2010, 37(3): 454-464. | |
[14] | 王梦姣. 内蒙古牧区马奶及其制品中肠球菌属乳酸菌安全性评价[D]. 呼和浩特: 内蒙古农业大学, 2014. |
WANG Mengjiao. Safety Evaluation of Lactic Acid Bacteria Isolated from Mare’s Milk and Its Products in Pastoral Areas of Inner Mongolia[D]. Hohhot: Inner Mongolia Agricultural University, 2014. | |
[15] | 孟丹, 王丽群, 谢国梁, 等. 一株乳酸乳球菌产γ-氨基丁酸能力及其安全性评价[J]. 中国酿造, 2017, 36(4): 72-77. |
MENG Dan, WANG Liqun, XIE Guoliang, et al. γ-aminobutyric acid-producing ability and safety evaluation of Lactococcus lactis[J]. China Brewing, 2017, 36(4): 72-77.
DOI |
|
[16] | 王瑞雪, 伊丽, 吉日木图. 驼乳生物活性肽的研究进展[J]. 中国食品学报, 2020, 20(7): 299-306. |
WANG Ruixue, YI Li, Jirimutu. Research progress on the bioactive peptides in camel milk[J]. Journal of Chinese Institute of Food Science and Technology, 2020, 20(7): 299-306. | |
[17] | 张七斤, 吕天星, 于晨龙, 等. 酸驼乳中益生乳酸菌的分离筛选[J]. 畜牧与饲料科学, 2017, 38(8): 1-5. |
ZHANG Qijin, LYU Tianxing, YU Chenlong, et al. Isolation and screen of probiotic lactic acid bacteria from traditional fermented camel milk[J]. Animal Husbandry and Feed Science, 2017, 38(8): 1-5. | |
[18] | 张苗苗. 新疆哈密地区原驼乳中乳酸菌多样性及产细菌素菌株的筛选[D]. 石河子: 石河子大学, 2019. |
ZHANG Miaomiao. Diversity of Lactic Acid Bacteria(LAB) in Raw Camel Milk in Hami Area of Xinjiang and Screening of Bacteriocin-Producing LAB Strains[D]. Shihezi: Shihezi University, 2019. | |
[19] | 任冬艳, 李磊, 李翠平, 等. 驼奶中乳酸菌的分离鉴定[J]. 食品安全导刊, 2021,(32): 96-98. |
REN Dongyan, LI Lei, LI Cuiping, et al. Isolation and identification of lactic acid bacteria from camel milk[J]. China Food Safety Magazine, 2021,(32): 96-98. | |
[20] |
Khedid K, Faid M, Mokhtari A, et al. Characterization of lactic acid bacteria isolated from the one humped camel milk produced in Morocco[J]. Microbiological Research, 2009, 164(1): 81-91.
PMID |
[21] | Jans C, Bugnard J, Njage P M K, et al. Lactic acid bacteria diversity of African raw and fermented camel milk products reveals a highly competitive, potentially health-threatening predominant microflora[J]. LWT, 2012, 47(2): 371-379. |
[22] | Saini K, Tomar S K. Invitro evaluation of probiotic potential of Lactobacillus cultures of human origin capable of selenium bioaccumulation[J]. LWT, 2017, 84: 497-504. |
[23] |
Ji K, Jang N Y, Kim Y T. Isolation of lactic acid bacteria showing antioxidative and probiotic activities from kimchi and infant feces[J]. Journal of Microbiology and Biotechnology, 2015, 25(9): 1568-1577.
DOI PMID |
[24] |
Reuben R C, Roy P C, Sarkar S L, et al. Isolation, characterization, and assessment of lactic acid bacteria toward their selection as poultry probiotics[J]. BMC Microbiology, 2019, 19(1): 253.
DOI PMID |
[25] | 蔡静静, 张亚川, 李谞, 等. 新疆伊犁地区乳制品中乳酸菌发酵和益生特性及其复合发酵方案优化[J]. 食品科学, 2020, 41(18): 172-179. |
CAI Jingjing, ZHANG Yachuan, LI Xu, et al. Fermentation and probiotic properties of lactic acid bacteria isolated from dairy products in Yili, Xinjiang and optimization of their mixtures for mixed-culture fermentation[J]. Food Science, 2020, 41(18): 172-179.
DOI |
|
[26] | LYU X, Hu P, Dang Y, et al. Purification and partial characterization of a novel bacteriocin produced by Lactobacillus casei TN-2 isolated from fermented camel milk (Shubat) of Xinjiang Uygur Autonomous Region, China[J]. Food Control, 2014, 43: 276-283. |
[27] | 王磊, 高宗露, 宗丽娜, 等. 酸乳用乳酸菌抗生素耐药性研究[J]. 食品研究与开发, 2021, 42(6): 162-169. |
WANG Lei, GAO Zonglu, ZONG Lina, et al. Study on antibiotic resistance of lactic acid bacteria for yoghurt[J]. Food Research and Development, 2021, 42(6): 162-169. | |
[28] | 万倩, 李启明, 吴华星, 等. 传统发酵食品中乳酸菌的安全性评估[J]. 现代食品科技, 2021, 37(6): 276-286. |
WAN Qian, LI Qiming, WU Huaxing, et al. Evaluation for lactic acid bacteria safety in traditional fermented food[J]. Modern Food Science and Technology, 2021, 37(6): 276-286. | |
[29] | 热孜姑丽·库尔班, 潘庆珉, 申彤, 等. 新疆阿勒泰地区传统酸驼乳中益生乳酸菌的体外筛选[J]. 食品科技, 2021, 46(7): 48-54. |
Reziguli Kuerban, PAN Qingmin, SHEN Tong, et al. In vitro screening of lactic acid bacteria with probiotic properties from spontaneously fermented camel milk in Altay, Xinjiang[J]. Food Science and Technology, 2021, 46(7): 48-54. | |
[30] | 李晓颖, 刘虹, 张颜廷, 等. 5株植物乳杆菌生物学特性及安全性评价[J]. 食品工业科技, 2017, 38(20): 115-119, 124. |
LI Xiaoying, LIU Hong, ZHANG Yanting, et al. Biological characteristics and safety evaluation of five Lactobacillus plantarum strains[J]. Science and Technology of Food Industry, 2017, 38(20): 115-119, 124. | |
[31] | Sadok I, Tyszczuk-Rotko K, Mroczka R, et al. Simultaneous voltammetric analysis of tryptophan and kynurenine in culture medium from human cancer cells[J]. Talanta, 2020, 209: 120574. |
[32] | 闫肃, 李慧敏, 张晓冬, 等. 不同食物来源乳酸细菌的安全性评价[J]. 食品与发酵工业, 2018, 44(5): 82-89. |
YAN Su, LI Huimin, ZHANG Xiaodong, et al. Safety assessment of lactic acid bacteria from different food products[J]. Food and Fermentation Industries, 2018, 44(5): 82-89.
DOI |
|
[33] |
Bertelli C, Pillonel T, Torregrossa A, et al. Bifidobacterium longum bacteremia in preterm infants receiving probiotics[J]. Clinical Infectious Diseases, 2015, 60(6): 924-927.
DOI PMID |
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