Xinjiang Agricultural Sciences ›› 2024, Vol. 61 ›› Issue (1): 199-208.DOI: 10.6048/j.issn.1001-4330.2024.01.022
• Agricultural Product Analysis and Detection·Forestry·Agricultural Information·Plant Protection • Previous Articles Next Articles
KONG Xiaoshuang1(), WEI Ran2, DONG Yinghong3, HOU Min3(
), Maierhaba Aihemaiti3, HOU Xinqiang3, YANG Wenqi3, CUI Weidong3(
)
Received:
2023-04-11
Online:
2024-01-20
Published:
2024-02-21
Correspondence author:
HOU Min(1983-),Urumqi,female,master,researcher, research direction in development and application of feed microorganism,(E-mail) Supported by:
孔晓双1(), 魏然2, 董应宏3, 侯敏3(
), 买尔哈巴·艾合买提3, 侯新强3, 杨文琦3, 崔卫东3(
)
通讯作者:
侯敏(1983-),女,新疆乌鲁木齐人,研究员,硕士,研究方向为饲用微生物开发与应用,(E-mail)作者简介:
孔晓双(1998-),女,河南周口人,硕士研究生,研究方向为食品工程,(E-mail)1940693886@qq.com
基金资助:
CLC Number:
KONG Xiaoshuang, WEI Ran, DONG Yinghong, HOU Min, Maierhaba Aihemaiti, HOU Xinqiang, YANG Wenqi, CUI Weidong. Isolation and screening of cultivable endophytes in sifferent organs of Glycyrrhiza uralensis Fisch and preliminary screening of β-glucosidase producing strains[J]. Xinjiang Agricultural Sciences, 2024, 61(1): 199-208.
孔晓双, 魏然, 董应宏, 侯敏, 买尔哈巴·艾合买提, 侯新强, 杨文琦, 崔卫东. 乌拉尔甘草不同组织可培养内生菌分离筛选及产β-葡萄糖苷酶菌株初筛[J]. 新疆农业科学, 2024, 61(1): 199-208.
部位 Part | 内生细菌 Endophytic bacteria | 内生真菌 Endophytic fungi | ||
---|---|---|---|---|
菌株数 Strain No.(个) | 百分率 Percentage(%) | 菌株数 Strain No.(个) | 百分率 Percentage(%) | |
主根 Taproot | 9 | 27.3 | 8 | 53.3 |
须根 Fibrous root | 8 | 24.2 | 1 | 6.7 |
茎 Stem | 9 | 27.3 | 4 | 26.7 |
叶 Leaf | 7 | 21.2 | 2 | 13.3 |
Tab.1 Results of endophyte isolation from different parts of Glycyrrhiza uralensis Fisch
部位 Part | 内生细菌 Endophytic bacteria | 内生真菌 Endophytic fungi | ||
---|---|---|---|---|
菌株数 Strain No.(个) | 百分率 Percentage(%) | 菌株数 Strain No.(个) | 百分率 Percentage(%) | |
主根 Taproot | 9 | 27.3 | 8 | 53.3 |
须根 Fibrous root | 8 | 24.2 | 1 | 6.7 |
茎 Stem | 9 | 27.3 | 4 | 26.7 |
叶 Leaf | 7 | 21.2 | 2 | 13.3 |
菌株 Strains | 部位 Part | 同源性最高的菌株 Closest species | 同源性 Samilarity(%) | GenBank登录号 GenBank accession No. |
---|---|---|---|---|
A12 | 主根 | Bacillus velezensis CR-502 | 99.43 | OP435752 |
A14 | 茎 | Fictibacillus nanhaiensis JSM 082006 | 99.16 | OP435753 |
A15 | 茎 | Bacillus amyloliquefaciens DSM 7 | 99.79 | OP435754 |
A17 | 叶 | Bacillus rugosus SPB7 | 99.52 | ON366397 |
A22 | 叶 | Bacillus velezensis CR-502 | 99.71 | OP435755 |
A23 | 须根 | Bacillus rugosus SPB7 | 97.38 | OP435756 |
A28 | 主根 | Bacillus velezensis CR-502 | 99.50 | OP435757 |
A29 | 主根 | Bacillus velezensis CR-502 | 99.43 | OP435758 |
A30 | 主根 | Bacillus subtilis 3610 | 99.20 | OP435759 |
A31 | 茎 | Bacillus cabrialesii TE3 | 99.11 | OP435760 |
A33 | 茎 | Bacillus tequilensis KCTC 13622 | 97.06 | OP435761 |
A34 | 茎 | Bacillus velezensis CR-502 | 99.29 | OP435762 |
A36 | 叶 | Janthinobacterium svalbardensis JA-1 | 99.36 | OP435763 |
A37 | 叶 | Bacillus tequilensis KCTC 13622 | 99.59 | OP435764 |
A41 | 须根 | Pseudomonas piscicoa dhj-6 | 99.92 | OP435765 |
A44 | 须根 | Bacillus tequilensis KCTC 13622 | 99.45 | OP435766 |
A45 | 须根 | Agrobacterium radiobacter ATCC19358 | 99.04 | OP435767 |
A62 | 主根 | Fictibacillus nanhaiensis JSM 082006 | 95.37 | OP435768 |
A63 | 主根 | Acinetobacter oryzae B23 | 98.95 | OP435769 |
A65 | 主根 | Bacillus subtilis 3610 | 99.14 | ON366398 |
A71 | 茎 | Fictibacillus nanhaiensisJSM 082006 | 99.16 | OP435770 |
A72 | 茎 | Bacillus cabrialesii TE3 | 99.31 | OP435771 |
A73 | 茎 | Metabacillus litoralis SW-211 | 94.55 | OP435772 |
A75 | 叶 | Niallia circulans ATCC 4513 | 99.24 | OP435773 |
A78 | 须根 | Bacillus rugosus SPB7 | 99.38 | ON366399 |
A79 | 主根 | Janthinobacterium svalbardensis JA-1 | 94.96 | OP435774 |
A80 | 主根 | Bacillus tequilensis KCTC 13622 | 99.66 | OP435775 |
A81 | 茎 | Bacillus tequilensis KCTC 13622 | 96.96 | OP435776 |
A82 | 叶 | Bacillus cabrialesii TE3 | 99.31 | OP435777 |
A83 | 叶 | Bacillus rugosus SPB7 | 99.31 | OP435778 |
A84 | 须根 | Bacillus mobilis 0711P9-1 | 99.52 | OP435779 |
A85 | 须根 | Bacillus tequilensis KCTC 13622 | 99.18 | OP435780 |
A86 | 须根 | Bacillus rugosus SPB7 | 96.83 | OP435781 |
Tab.2 Gene sequence analysis of endophytic bacteria from Glycyrrhiza uralensis Fisch
菌株 Strains | 部位 Part | 同源性最高的菌株 Closest species | 同源性 Samilarity(%) | GenBank登录号 GenBank accession No. |
---|---|---|---|---|
A12 | 主根 | Bacillus velezensis CR-502 | 99.43 | OP435752 |
A14 | 茎 | Fictibacillus nanhaiensis JSM 082006 | 99.16 | OP435753 |
A15 | 茎 | Bacillus amyloliquefaciens DSM 7 | 99.79 | OP435754 |
A17 | 叶 | Bacillus rugosus SPB7 | 99.52 | ON366397 |
A22 | 叶 | Bacillus velezensis CR-502 | 99.71 | OP435755 |
A23 | 须根 | Bacillus rugosus SPB7 | 97.38 | OP435756 |
A28 | 主根 | Bacillus velezensis CR-502 | 99.50 | OP435757 |
A29 | 主根 | Bacillus velezensis CR-502 | 99.43 | OP435758 |
A30 | 主根 | Bacillus subtilis 3610 | 99.20 | OP435759 |
A31 | 茎 | Bacillus cabrialesii TE3 | 99.11 | OP435760 |
A33 | 茎 | Bacillus tequilensis KCTC 13622 | 97.06 | OP435761 |
A34 | 茎 | Bacillus velezensis CR-502 | 99.29 | OP435762 |
A36 | 叶 | Janthinobacterium svalbardensis JA-1 | 99.36 | OP435763 |
A37 | 叶 | Bacillus tequilensis KCTC 13622 | 99.59 | OP435764 |
A41 | 须根 | Pseudomonas piscicoa dhj-6 | 99.92 | OP435765 |
A44 | 须根 | Bacillus tequilensis KCTC 13622 | 99.45 | OP435766 |
A45 | 须根 | Agrobacterium radiobacter ATCC19358 | 99.04 | OP435767 |
A62 | 主根 | Fictibacillus nanhaiensis JSM 082006 | 95.37 | OP435768 |
A63 | 主根 | Acinetobacter oryzae B23 | 98.95 | OP435769 |
A65 | 主根 | Bacillus subtilis 3610 | 99.14 | ON366398 |
A71 | 茎 | Fictibacillus nanhaiensisJSM 082006 | 99.16 | OP435770 |
A72 | 茎 | Bacillus cabrialesii TE3 | 99.31 | OP435771 |
A73 | 茎 | Metabacillus litoralis SW-211 | 94.55 | OP435772 |
A75 | 叶 | Niallia circulans ATCC 4513 | 99.24 | OP435773 |
A78 | 须根 | Bacillus rugosus SPB7 | 99.38 | ON366399 |
A79 | 主根 | Janthinobacterium svalbardensis JA-1 | 94.96 | OP435774 |
A80 | 主根 | Bacillus tequilensis KCTC 13622 | 99.66 | OP435775 |
A81 | 茎 | Bacillus tequilensis KCTC 13622 | 96.96 | OP435776 |
A82 | 叶 | Bacillus cabrialesii TE3 | 99.31 | OP435777 |
A83 | 叶 | Bacillus rugosus SPB7 | 99.31 | OP435778 |
A84 | 须根 | Bacillus mobilis 0711P9-1 | 99.52 | OP435779 |
A85 | 须根 | Bacillus tequilensis KCTC 13622 | 99.18 | OP435780 |
A86 | 须根 | Bacillus rugosus SPB7 | 96.83 | OP435781 |
菌株 Strains | 部位 Part | 同源性最高的菌株 Closest species | 同源性 Samilarity(%) | GenBank登录号 GenBank accession No. |
---|---|---|---|---|
p2 | 主根 | Penicillium sp. ST-PSB-J1 | 99.83 | OP681416 |
p3 | 主根 | Aspergillus fumigatus C1946 | 99.83 | OP681417 |
p4 | 主根 | Cladosporium cladosporioides 08SK030 | 92.03 | OP681418 |
p6 | 主根 | Alternaria alternata JN10 | 99.81 | OP681419 |
p9 | 茎 | Candolleomyces candolleanus NW550 | 99.42 | ON529501 |
p10 | 茎 | Penicillium cyclopium CICC 4026 | 99.15 | OP681420 |
p11 | 茎 | Cladosporium fusiforme a2 | 100.00 | OP681421 |
p12 | 茎 | Aspergillus fumigatus MUST | 99.15 | OP681422 |
p13 | 主根 | Cladosporium cladosporioides 08SK030 | 99.51 | OP681423 |
p15 | 须根 | Fusarium oxysporum NDJ2 | 99.65 | OP681424 |
p16 | 主根 | Aspergillus fumigatus MUST | 100.00 | OP681425 |
p17 | 叶 | Cladosporium perangustum IA39 | 97.42 | OP681426 |
p36 | 主根 | Trichothecium sp. 10731 | 99.42 | OP681427 |
p38 | 主根 | Alternaria alstroemeriae | 96.85 | OP681428 |
p44 | 叶 | Penicillium glabrum PM2 | 99.64 | OP681429 |
Tab.3 Gene sequence analysis of endophytic fungi in Glycyrrhiza uralensis Fisch
菌株 Strains | 部位 Part | 同源性最高的菌株 Closest species | 同源性 Samilarity(%) | GenBank登录号 GenBank accession No. |
---|---|---|---|---|
p2 | 主根 | Penicillium sp. ST-PSB-J1 | 99.83 | OP681416 |
p3 | 主根 | Aspergillus fumigatus C1946 | 99.83 | OP681417 |
p4 | 主根 | Cladosporium cladosporioides 08SK030 | 92.03 | OP681418 |
p6 | 主根 | Alternaria alternata JN10 | 99.81 | OP681419 |
p9 | 茎 | Candolleomyces candolleanus NW550 | 99.42 | ON529501 |
p10 | 茎 | Penicillium cyclopium CICC 4026 | 99.15 | OP681420 |
p11 | 茎 | Cladosporium fusiforme a2 | 100.00 | OP681421 |
p12 | 茎 | Aspergillus fumigatus MUST | 99.15 | OP681422 |
p13 | 主根 | Cladosporium cladosporioides 08SK030 | 99.51 | OP681423 |
p15 | 须根 | Fusarium oxysporum NDJ2 | 99.65 | OP681424 |
p16 | 主根 | Aspergillus fumigatus MUST | 100.00 | OP681425 |
p17 | 叶 | Cladosporium perangustum IA39 | 97.42 | OP681426 |
p36 | 主根 | Trichothecium sp. 10731 | 99.42 | OP681427 |
p38 | 主根 | Alternaria alstroemeriae | 96.85 | OP681428 |
p44 | 叶 | Penicillium glabrum PM2 | 99.64 | OP681429 |
Fig.4 The activity of β-glucosidase in fermentation broth of strains Note:Different lowercase letters indicate that the enzyme activities of endophytic bacteria or endophytic fungi are significantly different(P<0.05)
[1] |
Arora P, Wani Z A, Ahmad T, et al. Community structure,spatial distribution,diversity and functional characterization of culturable endophytic fungi associated with Glycyrrhiza glabra L.[J]. Fungal Biology, 2019, 123(5):373-383.
DOI URL |
[2] | 任群利, 王苗, 李小兰, 等. 黄芪内生菌多样性及其次生代谢产物研究进展[J]. 中国饲料, 2022,(15):13-18. |
REN Qun li, WANG Miao, LI Xiaolan, et al. Research progress on diversity and secondary metabolites of endophytic bacteria in Astragalus membranaceus[J]. China Feed, 2022,(15):13-18. | |
[3] |
张昊, 刘苗苗, 刘晓娜, 等. 内生菌影响药用植物产生药理活性化合物的研究进展[J]. 生物技术通报, 2022, 38(8):41-51.
DOI |
ZHANG Hao, LIU Miaomiao, LIU Xiaona, et al. Impact of Endophytic Microorganisms on the Pharmaco-active Compounds Production in Medicinal Plants:A Review[J]. Biotechnology Bulletin, 2022, 38(8):41-51.
DOI |
|
[4] |
Kusari P, Kusari S, Lamshöft M, et al. Quorum quenching is an antivirulence strategy employed by endophytic bacteria[J]. Applied Microbiology and Biotechnology, 2014, 98(16):7173-7183.
DOI PMID |
[5] | 马宗敏, 段绪红, 秦梦, 等. 微生物发酵技术在中药苷类生物转化中的应用进展[J]. 世界科学技术-中医药现代化, 2017, 13(5):858-864. |
MA Zongmin, DUAN Xuhong, QIN Meng, et al. Microbial fermentation technology in traditional Chinese medicine glycosides application progress in biotransformation[J]. World Science and Technology/Modernization of Traditional Chinese Medicine and Materia Medica, 2017, 13(5):858-864. | |
[6] |
梁金凤, 汪涯, 肖依文, 等. 内生真菌Eupenicillium javanicum R57水解京尼平苷β-葡萄糖苷酶的分离纯化及其酶学性质[J]. 菌物学报, 2017, 36(11):1543-1555.
DOI |
LIANG Jinfeng, WANG Ya, XIAO Yiwen, et al. Purification and characterization of geniposide- hydrolyzing β-glucosidase from endophytic Eupenicillium javanicum R57 harboring in Dongxiang wild rice[J]. Mycosystema, 2017, 36(11):1543-1555.
DOI |
|
[7] | 覃玲灵, 何钢, 陈介南. 里氏木霉及其纤维素酶高产菌株的研究进展[J]. 生物技术通报, 2011,(5):43-49. |
QIN Lingling, HE Gang, CHEN Jienan, et al. Research development of trichoderma reesei and its cellulase hyperproduction strains[J]. Biotechnology Bulletin, 2011,(5):43-49. | |
[8] |
Meysam M, Song G J, SunF B, et al. Positive role of non-catalytic proteins on mitigating inhibitory effects of lignin and enhancing cellulase activity in enzymatic hydrolysis:Application,mechanism and prospective[J]. Environmental Research, 2022, 215:114291.
DOI URL |
[9] | Ahmed A, Aslam M, Ashraf M, et al. Microbial β-Glucosidases:Screening,Characterization,Cloning and Applications[J]. Science and Education Publishing, 2017, 5(2):57-73. |
[10] |
刘宏丽, 郭晓军, 狄聪颖, 等. 产β-葡萄糖苷酶芽孢杆菌的筛选及水解大豆异黄酮糖苷研究[J]. 河北大学学报(自然科学版), 2017, 37(6):621-629.
DOI |
LIU Hongli, GUO Xiaojun, DI Congying, et al. Screening of bacillus strains producing β-Glucosidase and study on hydrolysis of soybean isoflavone glycosides[J]. Journal of Hebei University(Natural Science Ed.), 2017, 37(6):621-629. | |
[11] | 于洁. 虎杖内生真菌的分离筛选及其转化虎杖苷的研究[D]. 桂林: 桂林理工大学, 2020. |
YU Jie. Isolation and identification of endophytic fungi from Polygonum Cuspidatum and studies on the transformation of polydatin[D]. Guilin:Guilin University of Technology, 2020. | |
[12] |
Xie J C., Xu H., Jiang J C, et al. Characterization of a novel thermostable glucose-tolerant GH1 β-glucosidase from the hyperthermophile Ignisphaera aggregans and its application in the efficient production of baohuoside I from icariin and total epimedium flavonoids[J]. Bioorganic Chemistry, 2020, 104:104296.
DOI URL |
[13] |
Emran K C, Junhyun J, Soon O R, et al. Composition, diversity and bioactivity of culturable bacterial endophytes in mountain-cultivated ginseng in Korea[J]. Scientific Reports, 2017, 7(1):10098.
DOI |
[14] | Ahmed A, Nasim F, Batool K, et al. Microbial β-Glucosidase:Sources,Production and Applications[J]. Science and Education Publishing, 2017, 5(1):31-46. |
[30] | 刘姜华. 微生物转化槐角苷制备染料木素的研究[D]. 杭州: 浙江工业大学, 2017. |
LIU Jianghua. Production of genistein from sophoricoside by microbial transformation[D]. Hangzhou: Zhejiang University of Technology, 2017. | |
[31] | Tam N T, Tam N P, Nguyen V, et al. Isolation and screening of endophytic bacteria from Ngoc Linh ginseng(Panax vietnamensis Ha et Grushv) for biosynthesis β-glucosidase[J]. Vietnam Academy of Science and Technology, 2018, 40(2):153-161. |
[32] | Liu Y H, Guo J W, Salam N, et al. Culturable endophytic bacteria associated with medicinal plant Ferula songorica:molecular phylogeny,distribution and screening for industrially important traits[J]. 3 Biotech, 2016, 6(2):209. |
[33] | 张敏, 沈德龙, 饶小莉, 等. 甘草内生细菌多样性研究[J]. 微生物学通报, 2008, 35(4):524-528. |
ZHANG Min, SHEN Delong, RAO Xiaoli, et al. Diversity of Endophytic Bacteria isolated from Glycyrrhiza[J]. Microbiology China, 2008, 35(4):524-528. | |
[15] | 张媛媛, 苏敏, 朴春红, 等. 微生物来源的β-葡萄糖苷酶在食品工业中应用进展[J]. 食品工业科技, 2019, 40(16):329-335. |
ZHANG Yuanyuan, SU Min, PIAO Chunhong, et al. Progress on the β-Glucosidase from Microganisms and Its Applications in Food Industry[J]. Science and Technology of Food Industry, 2019, 40(16):329-335. | |
[16] | 杜晓宁. 宁夏枸杞可培养内生菌的多样性及其生物活性研究[D]. 银川: 宁夏大学, 2016. |
DU Xiaoning. Diversity and biological activity of endophytes isolated from Lycium barbarum of Ning xia[D]. Yinchuan: Ningxia University, 2016. | |
[17] |
Zhang J, Liu YX, Guo X, et al. High-throughput cultivation and identification of bacteria from the plant root microbiota[J]. Nature Protocols, 2021, 16(2):988-1012.
DOI PMID |
[18] | 刘文静, 程晗, 陈崇艺, 等. 产β-葡萄糖苷酶菌株的筛选及产酶条件优化[J]. 食品与发酵工业, 2019, 45(23):43-49. |
LIU Wenjing, CHENG Han, CHEN Chongyi, et al. Screening of β-glucosidase producing strains and optimization of enzyme production conditions[J]. Food and Fermentation Industries, 2019, 45(23):43-49. | |
[19] |
尤梦瑶, 万璐, 闫佳佳, 等. 甘草内生菌研究概况[J]. 中国农学通报, 2022, 38(26):20-26.
DOI |
YOU Mengyao, WAN Lu, YAN Jiajia, et al. Research Progress on Endophytes from Glycyrrhiza uralensis[J]. Chinese Agricultural Science Bulletin, 2022, 38(26):20-26.
DOI |
|
[20] | 曼琼, 杨志军, 邓毅, 等. 甘草内生菌的鉴定与药理作用和活性成分研究进展[J]. 中国临床药理学杂志, 2018, 34(9):1125-1128. |
MAN Qiong, YANG Zhijun, DENG Yi, et al. Research progress of identification,pharmacological action and active ingredients of endophytes isolated from Glycyrrhiza uralensis[J]. The Chinese Journal of Clinical Pharmacology, 2018, 34(9):1125-1128. | |
[21] | 陈静, 许贞, 张雪, 等. 不同产地甘草内生真菌多样性及分离条件研究[J]. 药学学报, 2019, 54(2):373-379. |
CHEN Jing, XU Zhen, ZHANG Xue, et al. Diversity and isolation parameters of endophytes from Glycyrrhiza uralensis of different habitats[J]. Acta Pharmaceutica Sinica, 2019, 54(2):373-379. | |
[22] | 张燃, 李佳. 宁夏野生甘草和种植甘草内生菌的分离和初鉴定[J]. 安徽农业科学, 2022, 50(13):169-171,178. |
ZHANG Ran, LI Jia. Isolation and preliminary identification of endophyte of wild and cultivated Glycyrrhiza uralensis in Ningxia[J]. Journal of Anhui Agricultural Sciences, 2022, 50(13):169-171,178. | |
[23] |
Li L, Mohamad O A A, Ma J, et al. Synergistic plant-microbe interactions between endophytic bacterial communities and the medicinal plant Glycyrrhiza uralensis F.[J]. Antonie Van Leeuwenhoek, 2018, 111(10):1735-1748.
DOI |
[24] | 陈昕, 李琪, 曹倩倩, 等. 麻花秦艽不同组织部位可培养内生菌群结构及其与龙胆苦苷含量的相关性[J]. 食品与生物技术学报, 2019, 38(4):21-29. |
CHEN Xin, LI Qi, CAO Qianqian, et al. Community Structure of Cultivable Endophytes in Different Organs of Gentiana straminea Maxim and Its Correlation with the Content of Gentiopicrin[J]. Journal of Food Science and Biotechnology, 2019, 38(4):21-29. | |
[25] |
Kaneko R, Kaneko S. The Effect of Bagging Branches on Levels of Endophytic Fungal Infection in Japanese Beech Leaves[J]. Forest Pathology, 2004, 34(2):65-78.
DOI URL |
[26] | Liu Z, Chen Y, Lian B, et al. Comparative Study on Population Ecological Distribution and Extracellular Enzyme Activities of Endophytic Fungi in Artemisia annua[J]. Journal of Bioscience and Medicine, 2019, 7(8):94-105. |
[27] | 张臣. 西洋参内生菌和根际微生物菌群结构分析及皂苷生物转化研究[D]. 郑州: 郑州大学, 2021. |
ZHANG Chen. Analysis of Structure Characteristics of Endogenous and soil microbial in Panax quinquefolius L. and Biotransformation Ginsenoside[D]. Zhengzhou: Zhengzhou University, 2021. | |
[28] | 张琴, 李艳宾, 李华. 产β-葡萄糖苷酶甘草内生菌的筛选及对甘草黄酮转化的研究[J]. 食品科学, 2013, 34(1):194-198. |
ZHANG Qin, LI Yanbin, LI Hua. Isolation of β-Glucosidase-Producing Endophytes from Glycyrrhiza inflate and Their Effects on Flavonoid Transformation[J]. Food Science, 2013, 34(1):194-198. | |
[29] |
Huang C, Feng Y, Patel G, et al. Production,immobilization and characterization of beta-glucosidase for application in cellulose degradation from a novel Aspergillus versicolor[J]. International Journal of Biological Macromolecules, 2021, 177:437-446.
DOI URL |
[1] | SUN Jian, LI Xue, CHU Min, GU Meiying, Ainijiang Ersiman, ZHU Jin, HE Qi, TAN Huilin, ZHANG Zhidong. Screening,identification and characteristics of Lactic acid bacteria from raw camel milk [J]. Xinjiang Agricultural Sciences, 2024, 61(4): 1021-1028. |
[2] | KANG Mintai, DU Xiaojing, ZHANG Yanhong, CHEN Yuhuan, WEN Xiaorong, TANG Fusen, ZHAO Zhiqiang, YUAN Jie, WANG Fengbin. Salt tolerance screening and fertility performance of rice varieties in saline areas of Xinjiang [J]. Xinjiang Agricultural Sciences, 2024, 61(3): 591-598. |
[3] | QI Ping, Suoyin Tuya, WEI Yang, ZHANG Shuo, Adili Shataer, Adili Aihemaiti. Evaluation of field control efficacy of 10 medications against Acalitus phloeocoptes Nalepa [J]. Xinjiang Agricultural Sciences, 2024, 61(11): 2761-2768. |
[4] | HE Qi, FENG Qian, LI Xue, YI Yuanyang, GU Meiying, ZHU Jin, SUN Jian, ZHANG Zhidong. Screening, identification and characteristics of Lactic acid bacteria from the sayram ketteki [J]. Xinjiang Agricultural Sciences, 2023, 60(9): 2323-2330. |
[5] | LU Tao, ZENG Qingtao, ZHANG Wen, WANG Wenbo, WANG Zhengyang, YANG Rui, SUN Yuyan. Comprehensive evaluation of cotton yield and quality by principal component analysis and grey correlation analysis [J]. Xinjiang Agricultural Sciences, 2023, 60(5): 1099-1109. |
[6] | Halidan Yikeremu, LIU Na, LIU Lianzheng, ZHOU Anding, JIANG Qiyan, Damailijiang Hezier, CAO Junmei, ZHANG Xinzhong. Identification and evaluation of salt tolerance in wheat relatives at germination and seedling stages [J]. Xinjiang Agricultural Sciences, 2023, 60(5): 1118-1126. |
[7] | DOU Ziwei, YANG Lu, CHENG Ping, ZHANG Zhigang, LI Hong. Analysis and Comprehensive Evaluation of Nutritional Quality of Different Mulberry Varieties [J]. Xinjiang Agricultural Sciences, 2023, 60(1): 127-139. |
[8] | ZHENG Ruiming, WANG Li, ZHANG Jungao, YAN Rong, LI Jin, LIANG Jing, LEI Bin, ZHOU Xiaoyun. Screening and Evaluation of Wheat Germination and Seedling Growth Activity by 16 Plant Methanol Extracts [J]. Xinjiang Agricultural Sciences, 2023, 60(1): 32-42. |
[9] | SAI Jingyi, WEN Yue, HAO Zhichao, TIAN Jia. Construction of Yeast Two-Hybrid Three-Frame cDNA Library and Screening of Interacting Proteins in FWL1 Membrane System of Pear Young Fruit [J]. Xinjiang Agricultural Sciences, 2022, 59(8): 1877-1888. |
[10] | RONG Hua, ZHANG Luhui, LIU Long, ZHENG Tongtong, LEI Bin, GUO Qingyuan. Study on Biological properties of Verticillium and Virulence Comparison of 16 Fungicides [J]. Xinjiang Agricultural Sciences, 2022, 59(8): 1984-1992. |
[11] | ZHANG Jianqiang, Abdramane Salah Zene, WANG Jianglai, LI Jiajia, ZHANG Xiaomeng, WU Kangli, TIAN Yongqinang. Selection of Agents for Alternaria tenuissima of Pathogen of Celery Leaf Spot [J]. Xinjiang Agricultural Sciences, 2022, 59(7): 1748-1757. |
[12] | LI Xiao, GUO Dongliang, LI Gongze, XUE Min, JIANG Haixia, YE Jiali, XIE Liqiong. Optimization of Salt Tolerance Identification System at Germination Stage of Flax and Comprehensive Assessment of Salt Tolerance of 150 Germplasms [J]. Xinjiang Agricultural Sciences, 2022, 59(6): 1438-1449. |
[13] | ZHANG Li, YANG Xiaolong, WANG Hailiang, NAN Shanshan, MA Xueer, NIE Cunxi, ZHANG Wenju, CHEN Cheng. Selection and Identification of a Cotton Phenol Degradation Strain and Its Detoxification Effect on Cotton Meal [J]. Xinjiang Agricultural Sciences, 2022, 59(12): 3057-3065. |
[14] | CHU Zhanyu, JIA Peisong, LUO Ying, Nurziya Yarmamat, FU Yongping, LI Yu. Efficacy Evaluation of Fungicides against Brown Rot of Pleurotus tuoliensis [J]. Xinjiang Agricultural Sciences, 2022, 59(11): 2682-2687. |
[15] | LIU Liya, YE Feng, MA Xiaojing, GU Wenxi, XIE Caiyun, ZHONG Qi, YI Xinping. Analysis of the Difference in Detecting Brucellosis with Different Initial Screening Methods and Different Diagnostic Reagents [J]. Xinjiang Agricultural Sciences, 2022, 59(1): 215-222. |
Viewed | ||||||
Full text |
|
|||||
Abstract |
|
|||||