Effects of exogenous 2, 4-epibrassinolide on seed germination of different salt-sensitive tomatoes under salt stress

doi:10.6048/j.issn.1001-4330.2024.08.019

Abstract

Abstract:

【Objective】 To study the effects of different concentrations of 2, 4-Epibrassinolide (EBR) on the germination characteristics and key enzyme activities of tomato seeds under salt stress and determine the optimal concentration of exogenous EBR for tomato seed germination and growth in the hope of providing theoretical support for the effective application of this plant growth regulator. 【Methods】 The seeds of salt-sensitive tomato ‘M82’ and salt-tolerant tomato ‘IL-7-5-5’ were treated with 50 mmol/L NaCl, and exogenous EBR of 0.01, 0.1 and 0.5 μmol/L were added to assess tomato seed germination stage of germination rate, germination potential, morphological characteristics, and analysis of antioxidant enzymes activity and osmotic regulation substances content changes. 【Results】 Compared with salt stress treatment, the germination potential of ‘M82’ and ‘IL-7-5-5’ after exogenous EBR treatment was increased by more than 10% to 30%, and the seed vigor index was increased by more than 100 to 200. With the increase of EBR concentration, the germination rate and germination potential of the two tomato varieties showed a decreasing trend. Compared with salt stress, the seed germ length and fresh weight of ‘M82’ treated with 0.01 μmol/L EBR increased by 94.26% and 135.71%, respectively. The germ length and fresh weight of ‘IL-7-5-5’ treated with 0.1 μmol/L EBR increased by 32.48% and 14.52%, respectively, compared with salt stress. The contents of osmoregulatory substances such as soluble protein and soluble sugar were significantly increased, and the activities of MDA, SOD, CAT and POD in the sprouts were significantly increased, but there were significant differences in physiological indexes between different EBR concentrations and different tomato varieties. 【Conclusion】 In the presence of salt stress, exogenous EBR can enhance the adaptability of tomato seeds to osmotic regulation during germination, improve the efficacy of antioxidant system in vivo, reduce the accumulation of membrane lipid peroxidation products, promote the germination and growth of tomato seeds, thus enhancing the tolerance of tomato seeds to salt during germination. 0.01 μmol/L EBR treatment has the best effect on ‘M82’ and 0.1 μmol/L on ‘IL-7-5-5’ under salt stress.

Key words: tomato; EBR; salt stress; seed germination

摘要：

【目的】研究盐胁迫下不同浓度2,4-表芸苔素内酯(2,4-Epibrassinolide,EBR)处理对番茄种子萌发特性和关键酶活性的影响,筛选影响番茄种子萌发及生长发育效果的最佳浓度,为外源EBR的科学应用提供理论依据。【方法】以盐敏感型‘M82’和耐盐型‘IL-7-5-5’番茄种子为材料,在50 mmol/L的NaCl处理下,添加0.01、0.1和0.5 μmol/L的外源EBR,测定番茄种子在萌发过程中的发芽率、发芽势等形态指标,分析抗氧化酶活性和渗透调节物质含量的变化。【结果】外源EBR处理后的‘M82’和‘IL-7-5-5’种子的发芽势较盐胁迫处理均提高了10%~30%以上,种子活力指数均提高了100~200以上,并且随着EBR浓度升高,2个番茄品种的发芽率、发芽势均呈下降的趋势。0.01 μmol/L EBR处理的‘M82’种子胚芽长和鲜重较盐胁迫下分别增加了94.26%和135.71%;0.1 μmol/L EBR处理的‘IL-7-5-5’胚芽长和鲜重较盐胁迫分别增加了32.48%和14.52%。幼芽体内MDA和SOD、CAT、POD活性显著提升,可溶性蛋白、可溶性糖等渗透调节物质含量显著增加,但不同EBR浓度、不同番茄品种之间的生理指标变化存在明显差异。【结论】外源EBR可以增强在盐胁迫条件下番茄种子萌发过程中调节渗透的能力,增强体内抗氧化系统的活性,膜脂过氧化产物的积累减少,促进番茄种子萌发和生长发育,提高番茄种子萌发期的耐盐性。盐胁迫下0.01 μmol/L的EBR处理对‘M82’的缓解效果最佳,0.1 μmol/L对‘IL-7-5-5’的缓解效果最佳。

关键词: 番茄, 2,4-表芸苔素内酯, 盐胁迫, 种子萌发

CLC Number:

S641.2

XI Rui, CHEN Yijia, LI Ning, YU Qinghui, WANG Qiang, QIN Yong. Effects of exogenous 2, 4-epibrassinolide on seed germination of different salt-sensitive tomatoes under salt stress[J]. Xinjiang Agricultural Sciences, 2024, 61(8): 1983-1992.

奚瑞, 陈怡佳, 李宁, 余庆辉, 王强, 秦勇. 外源2, 4-表芸苔素内酯对盐胁迫下不同盐敏感型番茄种子萌发的影响[J]. 新疆农业科学, 2024, 61(8): 1983-1992.

Figures/Tables 8

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处理 Treatments	胚根长 Radicle length(cm)		胚芽长 Plumule length(cm)		鲜重 Fresh weight(g)
处理 Treatments	‘M82’	‘IL-7-5-5’	‘M82’	‘IL-7-5-5’	‘M82’	‘IL-7-5-5’
CK	8.683±1.441^a	5.986±1.515^b	2.792±0.640^a	2.979±0.529^b	0.028±0.004^b	0.036±0.007^b
T₁	1.778±1.153^b	9.533±1.111^a	1.656±0.235^b	2.617±0.194^b	0.028±0.008^b	0.062±0.013^a
T₂	7.233±1.309^a	1.150±0.235^c	3.217±0.214^a	4.233±0.423^a	0.066±0.009^a	0.075±0.014^a
T₃	2.670±1.434^b	4.567±0.973^b	2.670±0.721^a	3.467±0.398^ab	0.058±0.038^a	0.071±0.010^a
T₄	0.983±0.360^b	4.500±1.028^b	3.233±0.163^a	3.817±0.417^a	0.059±0.006^a	0.071±0.007^a

处理 Treatments	胚根长 Radicle length(cm)		胚芽长 Plumule length(cm)		鲜重 Fresh weight(g)
处理 Treatments	‘M82’	‘IL-7-5-5’	‘M82’	‘IL-7-5-5’	‘M82’	‘IL-7-5-5’
CK	8.683±1.441^a	5.986±1.515^b	2.792±0.640^a	2.979±0.529^b	0.028±0.004^b	0.036±0.007^b
T₁	1.778±1.153^b	9.533±1.111^a	1.656±0.235^b	2.617±0.194^b	0.028±0.008^b	0.062±0.013^a
T₂	7.233±1.309^a	1.150±0.235^c	3.217±0.214^a	4.233±0.423^a	0.066±0.009^a	0.075±0.014^a
T₃	2.670±1.434^b	4.567±0.973^b	2.670±0.721^a	3.467±0.398^ab	0.058±0.038^a	0.071±0.010^a
T₄	0.983±0.360^b	4.500±1.028^b	3.233±0.163^a	3.817±0.417^a	0.059±0.006^a	0.071±0.007^a

处理 Treatments	盐害指数 Salt damage index(%)		盐害级别 Salt damage level
处理 Treatments	‘M82’	‘IL-7-5-5’	‘M82’	‘IL-7-5-5’
T₁	31.08	0.6	2	1
T₂	10.31	0	1	0
T₃	12.06	3.80	1	1
T₄	13.10	4.43	1	1

处理 Treatments	盐害指数 Salt damage index(%)		盐害级别 Salt damage level
处理 Treatments	‘M82’	‘IL-7-5-5’	‘M82’	‘IL-7-5-5’
T₁	31.08	0.6	2	1
T₂	10.31	0	1	0
T₃	12.06	3.80	1	1
T₄	13.10	4.43	1	1

番茄品种参数 Tomato varieties paramter	‘M82’		‘IL-7-5-5’
	提取成分 Extracted component		提取成分 Extracted component
	PC₁	PC₂	PC₁	PC₂
POD/CK	-4.516 74	0.023 612	-3.315 34	-0.123 07
POD/T₁	-2.818 11	0.024 878	-0.153 94	0.154 382
POD/T₂	-4.898 28	0.009 012	-3.138 23	-0.105 94
POD/T₃	-4.573 49	0.028 571	-4.630 61	0.241 061
POD/T₄	-0.905 18	-0.258 95	-5.738 05	-0.079 96
SOD/CK	0.592 356	0.023 681	0.606 218	0.001 529
SOD/T₁	0.522 783	0.010 684	0.656 309	-0.000 25
SOD/T₂	0.708 62	0.019 055	0.637 441	-0.000 25
SOD/T₃	0.692 586	-0.019 39	0.587 064	-0.01
SOD/T₄	0.652 105	0.013 944	0.656 031	-0.011 14
CAT/CK	0.667 488	0.009 894	0.653 886	-0.008 24
CAT/T₁	0.397 363	0.004 979	0.711 403	-0.009 52
CAT/T₂	0.597 567	0.012 894	0.550 948	0.003 602
CAT/T₃	0.510 239	0.006 908	0.583 378	-0.004 14
CAT/T₄	0.510 239	0.011 432	0.701 728	0.001 578
MAD/CK	0.753 945	0.005 247	0.690 946	-0.002 92
MAD/T₁	0.767 382	0.005 167	0.708 154	-0.003 18
MAD/T₂	0.775 85	0.004 66	0.703 684	-0.002 98
MAD/T₃	0.777 252	0.004 999	0.701 834	-0.002 94
MAD/T₄	0.769 486	0.005 383	0.709 295	-0.004 88
SP/CK	0.789 429	0.005 378	0.713 289	-0.003 37
SP/T₁	0.786 07	0.005 546	0.715 262	-0.003 18
SP/T₂	0.792 213	0.005 314	0.715 209	-0.003 43
SP/T₃	0.788 82	0.005 336	0.712 359	-0.003 42
SP/T₄	0.790 397	0.005 366	0.715 003	-0.003 43
SS/CK	0.787 845	0.005 372	0.702 173	-0.002 07
SS/T₁	0.788 676	0.005 395	0.708 79	-0.003 5
SS/T₂	0.787 871	0.004 966	0.710 174	-0.003 38
SS/T₃	0.789 014	0.005 402	0.713 701	-0.003 62
SS/T₄	0.790 074	0.005 263	0.711 894	-0.003 34