Effect of high temperature stress at anthesis on seed vigor of wheat

doi:10.6048/j.issn.1001-4330.2024.06.006

Abstract

Abstract:

【Objective】 To clarify the effect of post-anthesis high temperature treatment on grain germination and growth, and explore the psychological mechanism of wheat response to the stress of post-anthesis high temperature. 【Methods】 Xinchun 11, the main wheat cultivar in Xinjiang, was chosen as the model of crop. The mature wheat seeds were treated with the high temperature for 5 days to 8 days after flowering, and then the germination characteristics and the related enzymes activities associated with the oxidative stress of the seeds were examined. 【Results】 After high temperature treatment, the germination rate and germination potential of wheat grains were significantly reduced compared with the photo comparison (5.10% and 3.22%, respectively), and the conductivity index was significantly higher than that of the control (22.78% higher). After high temperature treatment, the bud length and fresh weight of wheat grain germination and growth decreased by 17.98% and 12.84% compared with the CK, the germination index and vigor index decreased significantly in wheat grain germination. The change in seed viability (ttc reduction intensity) was not significant, but the decrease was 26.02%. Meanwhile, the germination index and vigor index both decreased significantly in wheat grain germination. The change in seed viability (TTC reduction intensity) was not significant, but the decrease was 26.02%. 【Conclusion】 The results showed the high temperature stress at anthesis might have significant effects on wheat seed enzyme activity, and could further cause the decrease of the seed vigor and weaken the growth of wheat as a seedling, which might affect the wheat production ultimately.

Key words: wheat; seed germination; post-anthesis high temperature; amylase; peroxidase

摘要：

【目的】研究小麦花后高温处理对其籽粒发芽和生长的影响,分析小麦响应花后高温逆境的生理机制。【方法】以新疆主栽小麦品种新春11号为材料,取小麦成熟后的籽粒,待小麦开花后的5~8 d时进行高温处理,研究其萌发特性以及相关酶活性。【结果】小麦籽粒在经过高温处理后,籽粒发芽率和发芽势与对照相比较显著降低(分别降低5.10%和3.22%),电导率指标极显著高于对照(高出22.78%)。经过花后高温处理,小麦籽粒萌发并且长成嫩芽的芽长以及芽鲜重,与对照相比较显著降低17.98% 和12.84%;在小麦籽粒萌发中,发芽指数和活力指数均显著下降。种子活力(TTC还原强度)的变化虽然差异不显著,但降低程度达26.02%。【结论】小麦开花后遭受高温胁迫,导致籽粒中酶活性下降,最终造成种子活力降低,并且减缓了小麦幼苗时期的长势,小麦的产量亦受到影响。

关键词: 小麦, 花后高温, 籽粒萌发, 淀粉酶, 过氧化物酶

CLC Number:

S512

QIANG Lidong, FENG Kuan, ZHU Changan, ZHAO Yun, LI Zhaofeng, LI Weihua. Effect of high temperature stress at anthesis on seed vigor of wheat[J]. Xinjiang Agricultural Sciences, 2024, 61(6): 1345-1351.

强立栋, 冯宽, 朱长安, 赵云, 李召锋, 李卫华. 花后高温胁迫对小麦籽粒萌发及相关酶活性影响[J]. 新疆农业科学, 2024, 61(6): 1345-1351.

Figures/Tables 7

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处理 Treatments	幼苗长 Seedling length (cm)	幼苗鲜重 Seedling weight (mg)	发芽指数 Germination index.	活力指数Ⅰ Vigor index Ⅰ	活力指数Ⅱ Vigor index Ⅱ	种子活力 (TTC还原强度) Seed vigor (TTC restoration strength)
常温(CK)	22.51	120.66	155.97^*	3 510.88^*	18 819.34^*	1.668×10^-4
高温(HT)	19.71	114.66	120.22	2 368.33	13 784.42	1.234×10^-4

处理 Treatments	幼苗长 Seedling length (cm)	幼苗鲜重 Seedling weight (mg)	发芽指数 Germination index.	活力指数Ⅰ Vigor index Ⅰ	活力指数Ⅱ Vigor index Ⅱ	种子活力 (TTC还原强度) Seed vigor (TTC restoration strength)
常温(CK)	22.51	120.66	155.97^*	3 510.88^*	18 819.34^*	1.668×10^-4
高温(HT)	19.71	114.66	120.22	2 368.33	13 784.42	1.234×10^-4

相关系数 Correlation coefficient	活力指数Ⅱ Vigor index Ⅱ	发芽指数 Germination index	发芽势 Germination energy	发芽率 Germination percentage	淀粉酶活性 Amylase activity	POD活性 POD activity	CAT活性 CAT activity
活力指数Ⅰ Vigor indexⅠ	0.952^**	0.972^**	0.566^*	0.978^**	0.882^**	0.779^**	0.970^**
活力指数Ⅱ Vigor index Ⅱ		0.854^**	0.634^*	0.833^**	0.845^**	0.830^**	0.960^**
发芽指数 Germination index			0.645^*	0.968^**	0.683^*	0.989^**	0.832^**
发芽势 Germination energy				0.871^**	0.725^**	0.597^*	0.708^**
发芽率 Germination percentage					0.789^**	0.577^*	0.727^**
淀粉酶活性 Amylase activity						0.961^**	0.779^**
POD活性 POD activity							0.757^**

相关系数 Correlation coefficient	活力指数Ⅱ Vigor index Ⅱ	发芽指数 Germination index	发芽势 Germination energy	发芽率 Germination percentage	淀粉酶活性 Amylase activity	POD活性 POD activity	CAT活性 CAT activity
活力指数Ⅰ Vigor indexⅠ	0.952^**	0.972^**	0.566^*	0.978^**	0.882^**	0.779^**	0.970^**
活力指数Ⅱ Vigor index Ⅱ		0.854^**	0.634^*	0.833^**	0.845^**	0.830^**	0.960^**
发芽指数 Germination index			0.645^*	0.968^**	0.683^*	0.989^**	0.832^**
发芽势 Germination energy				0.871^**	0.725^**	0.597^*	0.708^**
发芽率 Germination percentage					0.789^**	0.577^*	0.727^**
淀粉酶活性 Amylase activity						0.961^**	0.779^**
POD活性 POD activity							0.757^**