Meta-analysis of influencing factors on soil microbial population in seasonal freeze-thaw period

doi:10.6048/j.issn.1001-4330.2024.05.022

Abstract

Abstract:

【Objective】To investigate the factors influencing soil bacterial and fungal populations during the seasonal freeze-thaw period.【Methods】The experimental data of relevant soil seasonal freeze-thaw periods were collected through the databases of China Knowledge Network and Web of Science, and the quality of the extracted literature was evaluated according to the exclusion and inclusion criteria and using the Newcastle-Ottawa Scale (NOS) evaluation criteria, and those with scores ≥6 and high quality were included in the study, and RevMan 5.4 was used for Meta-analysis.【Results】The final 42 papers met the inclusion criteria, among which, 7 were SCI articles and 27 were CSCD articles.The analysis showed that soil water content had the most significant effect on soil bacteria during the seasonal freeze-thaw period; soil pH had the most significant effect on soil fungi, and soil organic matter had the least significant effect on soil bacteria and fungi.During the seasonal freeze-thaw period, soil organic nitrogen had the most dominant effect on soil bacteria soil water content had the most dominant effect on soil fungi.【Conclusion】Soil pH and soil water content play a decisive role in soil bacteria and fungi during the seasonal freeze-thaw period.

Key words: Meta-analysis; soil bacteria; soil fungus; seasonal freeze-thaw

摘要：

【目的】研究季节性冻融期土壤细菌和真菌数量的影响因素。【方法】采集中国知网、Web of science等数据库相关土壤季节性冻融期的数据,根据排除和纳入标准,采用Newcastle-Ottawa Scale (NOS)评价标准对文献进行质量评价,将得分≥6分且高质量的文献纳入研究,运用RevMan5.4对其进行Meta分析。【结果】42篇文献符合纳入标准,其中,SCI文章7篇,CSCD文章27篇。季节性冻融期土壤含水量对土壤细菌的影响显著性最大;土壤pH值对土壤真菌的影响显著性最大,土壤有机质对土壤细菌和真菌影响显著性最小。季节性冻融期,土壤有机氮对土壤细菌影响的优势程度最大,土壤含水量对土壤真菌影响的优势程度最大。【结论】季节性冻融期土壤pH值和土壤含水量对土壤细菌和真菌起到决定性作用。

关键词: Meta分析, 土壤细菌, 土壤真菌, 季节性冻融期

CLC Number:

S188

YANG Yuefa, WANG Chunxia, LIANG Fei, LAN Mingju. Meta-analysis of influencing factors on soil microbial population in seasonal freeze-thaw period[J]. Xinjiang Agricultural Sciences, 2024, 61(5): 1236-1249.

杨跃发, 王春霞, 梁飞, 蓝明菊. 季节性冻融期土壤微生物的数量影响因素Meta分析[J]. 新疆农业科学, 2024, 61(5): 1236-1249.

Figures/Tables 14

References 71

[1]	Ji X M, Liu M H, Yang J L, et al. Meta-analysis of the impact of freeze-thaw cycles on soil microbial diversity and C and N dynamics[J]. Soil Biology and Biochemistry, 2022.
[2]	Kennedy A C, Smith K L. Soil microbial diversity and the sustainability of agricultural soils[J]. Plant and Soil, 1995, 170(1): 75-86.
[3]	方圆, 王娓, 姚晓东, 等. 我国北方温带草地土壤微生物群落组成及其环境影响因素[J]. 北京大学学报(自然科学版), 2017, 53(1): 142-150.
	FANG Yuan, WANG Wei, YAO Xiaodong, et al. Soil microbial community composition and environmental controls in northern temperate steppe of China[J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2017, 53(1): 142-150.
[4]	蒋双龙, 胡玉福, 蒲琴, 等. 川西北高寒草地沙化过程中土壤氮素变化特征[J]. 生态学报, 2016, 36(15): 4644-4653.
	JIANG Shuanglong, HU Yufu, PU Qin, et al. Changes in soil nitrogen characteristics during grassland desertification in Northwest Sichuan[J]. Acta Ecologica Sinica, 2016, 36(15): 4644-4653.
[5]	Brooks P D, Grogan P, Templer P H, et al. Carbon and nitrogen cycling in snow-covered environments[J]. Geography Compass, 2011, 5(9): 682-699.
[6]	Campbell J L, Ollinger S V, Flerchinger G N, et al. Past and projected future changes in snowpack and soil frost at the Hubbard Brook Experimental Forest, New Hampshire, USA[J]. Hydrological Processes, 2010, 24(17): 2465-2480.
[7]	Muller S W. Permafrost; or, Permanently frozen ground and related engineering problems[M]. Ann Arbor: J. W. Edwards, 1947
[8]	韩露, 万忠梅, 孙赫阳. 冻融作用对土壤物理、化学和生物学性质影响的研究进展[J]. 土壤通报, 2018, 49(3): 736-742.
	HAN Lu, WAN Zhongmei, SUN Heyang. Research progress on the effects of freezing and thawing on soil physical, chemical and biological properties[J]. Chinese Journal of Soil Science, 2018, 49(3): 736-742.
[9]	Finegold L. Molecular and biophysical aspects of adaptation of life to temperatures below the freezing point[J]. Advances in Space Research, 1996, 18(12): 87-95.
[10]	马晓飞, 楚新正, 马倩. 艾比湖地区融雪期梭梭和柽柳林地土壤酶活性研究[J]. 中国农学通报, 2016, 32(14): 138-145. DOI
	MA Xiaofei, CHU Xinzheng, MA Qian. Soil enzyme activity of Haloxylon ammondendron and Tamarix chinensis forest land in ebinur lake region during snow melting period[J]. Chinese Agricultural Science Bulletin, 2016, 32(14): 138-145.
[11]	刘利. 季节性冻融对亚高山/高山森林土壤微生物多样性的影响[D]. 雅安: 四川农业大学, 2010.
	LIU Li. Soil Bacterial Diversity in the Subalpine/Alpine Forests of Western Sichuan Affected by Seasonal Freeze-thaw Cycles[D]. Yaan: Sichuan Agricultural University, 2010.
[12]	Sulkava P, Huhta V. Effects of hard frost and freeze-thaw cycles on decomposer communities and N mineralisation in boreal forest soil[J]. Applied Soil Ecology, 2003, 22(3): 225-239
[13]	Hedges L V, Gurevitch J, Curtis P S. The meta-analysis of response ratios in experimental ecology[J]. Ecology, 1999, 80(4): 1150.
[14]	秦璐, 吕光辉, 何学敏. 艾比湖地区冻融作用对土壤微生物数量和群落结构的影响[J]. 冰川冻土, 2013, 35(6): 1590-1599. DOI
	QIN Lu, LYU Guanghui, HE Xuemin. Effects of freezing-thawing on soil microbial quantity and community structure around the ebinur lake[J]. Journal of Glaciology and Geocryology, 2013, 35(6): 1590-1599. DOI
[15]	Greenland S. Quantitative methods in the review of epidemiologic literature[J]. Epidemiologic Reviews, 1987, 9: 1-30. PMID
[16]	马晓飞, 楚新正, 马倩. 艾比湖地区冻融作用对梭梭群落土壤酶活性及微生物数量的影响[J]. 干旱区地理, 2015, 38(6): 1190-1201.
	MA Xiaofei, CHU Xinzheng, MA Qian. Soil enzyme activity and microbial biomass of Haloxylon ammodendron community under freeze-thaw action[J]. Arid Land Geography, 2015, 38(6): 1190-1201.
[17]	杨晓. 北极新奥尔松地区土壤微生物多样性初步分析[D]. 青岛: 青岛大学, 2016.
	YANG Xiao. Preliminary Analysis of Soil Microbial Diversity in Ny-Alesund, Arctic[D]. Qingdao: Qingdao University, 2016.
[18]	王鹭, 董小培, 张威, 等. 不同深度冻土微生物数量特征及其与土壤理化性质的关系[J]. 冰川冻土, 2011, 33(2): 436-441.
	WANG Lu, DONG Xiaopei, ZHANG Wei, et al. Quantitative characters of microorganisms in permafrost at different depths and their relation to soil physicochemical properties[J]. Journal of Glaciology and Geocryology, 2011, 33(2): 436-441.
[19]	伍文宪, 张蕾, 黄小琴, 等. 川西北高寒牧区不同人工草地对土壤微生物多样性影响[J]. 草业学报, 2019, 28(3): 29-41. DOI
	WU Wenxian, ZHANG Lei, HUANG Xiaoqin, et al. Difference in soil microbial diversity in artificial grasslands of the Northwest Plateau of Sichuan Province[J]. Acta Prataculturae Sinica, 2019, 28(3): 29-41. DOI
[20]	王楠, 王传宽, 邸雪颖, 等. 春季冻融期兴安落叶松林土壤微生物的时间动态及其影响因子[J]. 应用生态学报, 2019, 30(8): 2757-2766. DOI
	WANG Nan, WANG Chuankuan, DI Xueying, et al. Temporal dynamics and influencing factors of soil microbes in Larix gmelinii forest soil during spring freezing-thawing period[J]. Chinese Journal of Applied Ecology, 2019, 30(8): 2757-2766. DOI
[21]	余炎炎, 李梦莎, 刘啸林, 等. 大兴安岭典型永久冻土土壤细菌群落组成和多样性[J]. 微生物学通报, 2020, 47(9): 2759-2770.
	YU Yanyan, LI Mengsha, LIU Xiaolin, et al. Soil bacterial community composition and diversity of typical permafrost in Greater Khingan Mountains[J]. Microbiology China, 2020, 47(9): 2759-2770.
[22]	孙弘哲, 马大龙, 臧淑英, 等. 大兴安岭多年冻土区不同林型土壤微生物群落特征[J]. 冰川冻土, 2018, 40(5): 1028-1036. DOI
	SUN Hongzhe, MA Dalong, ZANG Shuying, et al. Characteristics of soil microbial community structure under different forest types of permafrost regions in the Greater Khingan Mountains[J]. Journal of Glaciology and Geocryology, 2018, 40(5): 1028-1036. DOI
[23]	车子涵, 陈克龙, 杜岩功, 等. 冻融凹陷对瓦颜山河源湿地土壤微生物群落结构的影响[J]. 基因组学与应用生物学, 2023, 42(2): 204-216.
	CHE Zihan, CHEN Kelong, DU Yangong, et al. Effect of freeze-thaw depression on soil microbial community structure in wayanshan Heyuan wetland[J]. Genomics and Applied Biology, 2023, 42(2): 204-216.
[24]	段亚楠, 刘恩太, 陈学森, 等. 冻融对老龄苹果园土壤微生物数量及酶活性的影响[J]. 土壤, 2021, 53(1): 125-132.
	DUAN Yanan, LIU Entai, CHEN Xuesen, et al. Effects of freezing-thawing on soil microbial quantities and enzymatic activities of old apple orchards[J]. Soils, 2021, 53(1): 125-132.
[25]	陈末, 朱新萍, 蒋靖佰伦, 等. 冻融期巴音布鲁克高寒湿地土壤细菌群落变化及其响应机制[J]. 农业环境科学学报, 2020, 39(1): 134-142.
	CHEN Mo, ZHU Xinping, JIANG Jingbailun, et al. Shifts in soil bacterial community and their response mechanism during freeze-thaw period in Bayinbuluk alpine wetland[J]. Journal of Agro-Environment Science, 2020, 39(1): 134-142.
[26]	林尤伟, 金光泽. 冻融期去根处理对小兴安岭6种林型土壤微生物量的影响[J]. 生态学报, 2016, 36(19): 6159-6169.
	LIN Youwei, JIN Guangze. Effects of root resectioning on soil microbial biomass in six forest types in the Xiaoxing’an Mountains during freezing-thawing cycles[J]. Acta Ecologica Sinica, 2016, 36(19): 6159-6169.
[27]	张宝贵, 张威, 刘光琇, 等. 冻融循环对青藏高原腹地不同生态系统土壤细菌群落结构的影响[J]. 冰川冻土, 2012, 34(6): 1499-1507.
	ZHANG Baogui, ZHANG Wei, LIU Guangxiu, et al. Effect of freeze-thaw cycles on the soil bacterial communities in different ecosystem soils in the Tibetan Plateau[J]. Journal of Glaciology and Geocryology, 2012, 34(6): 1499-1507.
[28]	李君锋, 杨建文, 杨婷婷, 等. 甘肃玛曲高寒草甸土壤微生物季节变化特性的研究[J]. 草业科学, 2012, 29(2): 189-197.
	LI Junfeng, YANG Jianwen, YANG Tingting, et al. Seasonal dynamics of soil microbes and their relationship with soil physicochemical factors in alpine meadow in Maqu of Gansu[J]. Pratacultural Science, 2012, 29(2): 189-197.
[29]	关健飞, 曹阳. 黑龙江省表层冻土细菌群落结构组成和功能特征[J]. 生态学报, 2020, 40(14): 4929-4941.
	GUAN Jianfei, CAO Yang. Bacterial community structure analysis of surface frozen soil in Heilongjiang Province[J]. Acta Ecologica Sinica, 2020, 40(14): 4929-4941.
[30]	马大龙, 刘梦洋, 陈泓硕, 等. 积雪覆盖变化对大兴安岭多年冻土区土壤微生物群落结构的影响[J]. 生态学报, 2020, 40(3): 789-799.
	MA Dalong, LIU Mengyang, CHEN Hongshuo, et al. Effects of snow cover change on soil microbial community structure in permafrost region of Great Hing’an Mountains[J]. Acta Ecologica Sinica, 2020, 40(3): 789-799.
[31]	陈泓硕, 马大龙, 姜雪薇, 等. 季节性冻融对扎龙湿地土壤微生物群落结构和胞外酶活性的影响[J]. 环境科学学报, 2020, 40(4): 1443-1451.
	CHEN Hongshuo, MA Dalong, JIANG Xuewei, et al. Effects of seasonal freeze-thaw on soil microbial community structures and extracellular enzyme activities in Zhalong wetland[J]. Acta Scientiae Circumstantiae, 2020, 40(4): 1443-1451.
[32]	李昌明, 张新芳, 赵林, 等. 青藏高原多年冻土区土壤需氧可培养细菌多样性及群落功能研究[J]. 冰川冻土, 2012, 34(3): 713-725.
	LI Changming, ZHANG Xinfang, ZHAO Lin, et al. Phylogenetic diversity of bacteria isolates and community function in permafrost-affected soil along different vegetation types in the Qinghai-Tibet Plateau[J]. Journal of Glaciology and Geocryology, 2012, 34(3): 713-725.
[33]	张宝贵, 刘晓娇, 吴青柏, 等. 青藏高原昆仑山垭口不同深度土壤可培养细菌群落特征研究[J]. 冰川冻土, 2016, 38(3): 776-784. DOI
	ZHANG Baogui, LIU Xiaojiao, WU Qingbai, et al. Research of the soil bacteria community characteristics at different depths in Kunlun Mountains Pass, Tibetan Plateau[J]. Journal of Glaciology and Geocryology, 2016, 38(3): 776-784. DOI
[34]	董康, 李师翁, 康文龙, 等. 青藏公路沿线土壤微生物数量变化及其影响因素研究[J]. 冰川冻土, 2013, 35(2): 457-464. DOI
	DONG Kang, LI Shiweng, KANG Wenlong, et al. Study of the changes in microbe amount and its affect factors in the soils along the qinghai-tibet highway[J]. Journal of Glaciology and Geocryology, 2013, 35(2): 457-464. DOI
[35]	张宝贵, 刘晓娇, 刘敏, 等. 疏勒河上游不同退化类型冻土可培养细菌数量变化[J]. 生态学杂志, 2017, 36(10): 2886-2893.
	ZHANG Baogui, LIU Xiaojiao, LIU Min, et al. Change of culturable bacterial quantity in different types of degraded permafrost in the upstream region of Shule River Basin[J]. Chinese Journal of Ecology, 2017, 36(10): 2886-2893.
[36]	Song D D, Cui Y Q, Ma D L, et al. Spatial variation of microbial community structure and its driving environmental factors in two forest types in permafrost region of greater Xing’an Mountains[J]. Sustainability, 2022, 14(15): 9284.
[37]	胡霞, 尹鹏, 彭言劼, 等. 冻融交替下高山土壤微生物和矿质氮库对外源氮输入的响应[J]. 重庆师范大学学报(自然科学版), 2021, 38(4): 121-128.
	HU Xia, YIN Peng, PENG Yanjie, et al. Response of mineral nitrogen pool and microorganism to external nitrogen input in alpine soil under freeze-thaw alternation[J]. Journal of Chongqing Normal University (Natural Science), 2021, 38(4): 121-128.
[38]	孙嘉鸿, 郭彤, 董彦民, 等. 冻融循环对金川泥炭沼泽土壤微生物量及群落结构的影响[J]. 生态学报, 2022, 42(7): 2763-2773.
	SUN Jiahong, GUO Tong, DONG Yanmin, et al. Effect of freezing and thawing on soil microbial biomass and community structure in Jinchuan peatlands[J]. Acta Ecologica Sinica, 2022, 42(7): 2763-2773.
[39]	张超凡, 盛连喜, 宫超, 等. 冻融作用对我国东北湿地土壤碳排放与土壤微生物的影响[J]. 生态学杂志, 2018, 37(2): 304-311.
	ZHANG Chaofan, SHENG Lianxi, GONG Chao, et al. Effects of freeze-thaw cycles on soil microbial biomass carbon and carbon emissions from wetland soils, Northeast China[J]. Chinese Journal of Ecology, 2018, 37(2): 304-311.
[40]	薛烨飞. 季节性冻融对农业排水渠湿地植物根系周围底泥环境及微生物的影响[D]. 长春: 东北师范大学, 2021.
	XUE Yefei. Effects of seasonal freeze-thaw on the sediment environment and microbial communities around the roots of wetland plants in an agricultural drainage ditch[D]. Changchun: Northeast Normal University, 2021.
[41]	王奥. 季节性冻融对高山森林土壤微生物与生化特性的影响[D]. 雅安: 四川农业大学, 2012.
	WANG Ao. Effect of Seasonal Freeze-thaw on Soil Microbial and Biochemical Property in Alpine Forest Soil[D]. Yaan: Sichuan Agricultural University, 2012.
[42]	李淼, 冯海艳, 杨忠芳, 等. 中国典型冻土区土壤可培养细菌多样性[J]. 微生物学报, 2011, 51(12): 1595-1604.
	LI Miao, FENG Haiyan, YANG Zhongfang, et al. Diversity of culturable bacteria in the typical frozen soil areas in China[J]. Acta Microbiologica Sinica, 2011, 51(12): 1595-1604. PMID
[43]	姜霁珊. 长白山土壤微生物对土壤基本理化性质的响应研究——冻融期不同林型[D]. 延吉: 延边大学, 2019.
	JIANG Jishan. Response Study of Soil Microorganisms to Soil Basic Physical and Chemical Properties in Changbai Mountain[D]. Yanji: Yanbian University, 2019.
[44]	刘超, 王宪伟, 宋艳宇, 等. 增温对冻土区泥炭沼泽土壤孔隙水甲烷关联微生物和溶解性有机碳的影响[J]. 生态学报, 2021, 41(1): 184-193.
	LIU Chao, WANG Xianwei, SONG Yanyu, et al. Effects of warming on abundances of methane-related microorganisms and concentration of dissolved organic carbon in soil pore water of permafrost peat swamp in Daxing’anling[J]. Acta Ecologica Sinica, 2021, 41(1): 184-193.
[45]	王艳发, 魏士平, 崔鸿鹏, 等. 祁连山冻土区土壤活动层与冻土层中甲烷代谢微生物群落结构特征[J]. 应用与环境生物学报, 2016, 22(4): 592-598.
	WANG Yanfa, WEI Shiping, CUI Hongpeng, et al. Methane metabolic microbial community structure in the active layer and the permafrost layer of the Qilian permafrost, China[J]. Chinese Journal of Applied and Environmental Biology, 2016, 22(4): 592-598.
[46]	孙秦川. 青藏高原冻土区石油污染对土壤微生物多样性的影响研究[D]. 兰州: 兰州交通大学, 2017.
	SUN Qinchuan. Effects of Oil Pollution on Soil Microbial Diversity in the Qinghai-tibet Plateau Permafrost Regions[D]. Lanzhou: Lanzhou Jiatong University, 2017.
[47]	吴明辉, 瞿德业, 李婷, 等. 祁连山疏勒河源区冻土退化对土壤微生物生物量碳氮的影响[J]. 地理科学, 2021, 41(1): 177-186. DOI
	WU Minghui, QU Deye, LI Ting, et al. Effects of permafrost degradation on soil microbial biomass carbon and nitrogen in the Shule River headwaters, the Qilian Mountains[J]. Scientia Geographica Sinica, 2021, 41(1): 177-186. DOI
[48]	胡维刚. 昆仑山垭口深层多年冻土微生物多样性及构建机制研究[D]. 兰州: 兰州大学, 2016.
	HU Weigang. Microbial Diversity And Community Assembly in Permafrost Soils from The Kunlun Mountain Pass[D]. Lanzhou: Lanzhou University, 2016.
[49]	谭波, 吴福忠, 秦嘉励, 等. 川西亚高山、高山森林土壤微生物生物量和酶活性动态特征[J]. 生态环境学报, 2014, 23(8): 1265-1271.
	TAN Bo, WU Fuzhong, QIN Jiali, et al. Dynamics of soil microbial biomass and enzyme activity in the subalpine/alpine forests of western Sichuan[J]. Ecology and Environmental Sciences, 2014, 23(8): 1265-1271.
[50]	谭波. 长江上游不同海拔代表性森林土壤动物对凋落叶分解的影响[D]. 雅安: 四川农业大学, 2013.
	TAN Bo. Effects of Soil Fauna on Forest Litter Decomposition in the Upper Reaches of the Yangtze River[D]. Yaan: Sichuan Agricultural University, 2013.
[51]	Sorensen P O, Finzi A C, Giasson M A, et al. Winter soil freeze-thaw cycles lead to reductions in soil microbial biomass and activity not compensated for by soil warming[J]. Soil Biology and Biochemistry, 2018, 116: 39-47.
[52]	Grant J, Konrad K, Fereidoun R, et al. Microbial community compositional stability in agricultural soils during freeze-thaw and fertilizer stress[J]. Frontiers in Environmental Science, 2022, 10.
[53]	Meisner Annelein, Basten L, Snoek, et al. Soil microbial legacies differ following drying rewetting and freezing-thawing cycles[J]. The ISME Journal, 2021, 15(4): 1207-1221.
[54]	Tsunehiro Watanabe, Ryunosuke Tateno, Shogo Imada, et al. The effect of a freeze-thaw cycle on dissolved nitrogen dynamics and its relation to dissolved organic matter and soil microbial biomass in the soil of a northern hardwood forest[J]. Biogeochemistry, 2019, 142(3): 319-338. DOI
[55]	Hu X, Yin P, Nong X, et al. Effect of exogenous carbon addition and the freeze-thaw cycle on soil microbes and mineral nitrogen pools1[J]. IOP Conference Series: Earth and Environmental Science, 2018, 108: 032046.
[56]	Wu H, Dannenmann M, Wolf B, et al. Seasonality of soil microbial nitrogen turnover in continental steppe soils of Inner Mongolia[J]. Ecosphere, 2012, 3(4): 1-18.
[57]	Gilichinsky D A. Permafrost model of extraterrestrial habitat[M]. Horneck G, Baumstark-Khan C. Astrobiology. Berlin, Heidelberg: Springer, 2002: 125-142.
[58]	周璟, 盛红梅, 安黎哲. 极端微生物的多样性及应用[J]. 冰川冻土, 2007, 29(2): 286-291.
	ZHOU Jing, SHENG Hongmei, AN Lizhe. Diversity of extremophilic miroorganisms and their applications[J]. Journal of Glaciology and Geocryology, 2007, 29(2): 286-291.
[59]	杨思忠, 金会军, 魏智, 等. 微生物对冻土生境的适应以及对全球变化和寒区工程扰动的响应: 进展与展望[J]. 冰川冻土, 2007, 29(2): 279-285.
	YANG Sizhong, JIN Huijun, WEI Zhi, et al. Microbial adaptation to the habitat of permafrost and their responses to global change and engineering disturbance in cold regions: advances and prospects[J]. Journal of Glaciology and Geocryology, 2007, 29(2): 279-285.
[60]	Chu H Y, Fierer N, Lauber C L, et al. Soil bacterial diversity in the Arctic is not fundamentally different from that found in other biomes[J]. Environmental Microbiology, 2010, 12(11): 2998-3006. DOI PMID
[61]	Larsen K S, Jonasson S, Michelsen A. Repeated freeze-thaw cycles and their effects on biological processes in two Arctic ecosystem types[J]. Applied Soil Ecology, 2002, 21(3): 187-195.
[62]	张立新, 韩文玉, 顾同欣. 冻融过程对景电灌区草窝滩盆地土壤水盐动态的影响[J]. 冰川冻土, 2003, 25(3): 297-302.
	ZHANG Lixin, HAN Wenyu, GU Tongxin. The influence of freezing and thawing on the moisture-salt activity of soil in caowotan basin of jingdian irrigated area[J]. Journal of Glaciology and Geocryology, 2003, 25(3): 297-302.
[63]	杨思忠, 金会军. 冻融作用对冻土区微生物生理和生态的影响[J]. 生态学报, 2008, 28(10): 5065-5074.
	YANG Sizhong, JIN Huijun. Physiological and ecological effects of freezing and thawing processes on microorganisms in seasonally-froze ground and in permafrost[J]. Acta Ecologica Sinica, 2008, 28(10): 5065-5074.
[64]	Mazur P. Cryobiology: the freezing of biological systems[J]. Science, 1970, 168(3934): 939-949. PMID
[65]	杨玉莲, 吴福忠, 何振华, 等. 雪被去除对川西高山冷杉林冬季土壤微生物生物量碳氮和可培养微生物数量的影响[J]. 应用生态学报, 2012, 23(7): 1809-1816.
	YANG Yulian, WU Fuzhong, HE Zhenhua, et al. Effects of snow pack removal on soil microbial biomass carbon and nitrogen and the number of soil culturable microorganisms during wintertime in alpine Abies faxoniana forest of western Sichuan, Southwest China[J]. Chinese Journal of Applied Ecology, 2012, 23(7): 1809-1816. PMID
[66]	Jiang L, Yue K, Yang Y. l. Leaching and freeze-thaw events contribute to litter decomposition a review[J]. Sains Malaysiana, 2016, 45(7): 1041-1047.
[67]	Groffman P M, Drisoll C T. Effects of mild winter freezing on soil nitrmgen and carbon dynamics in a northern hardwood forest[J]. Biogeochemistry, 2001, 56(2): 191-213.
[68]	吴金水, 林启美, 黄巧云. 土壤微生物生物量测定方法及其应用[M]. 北京: 气象出版社, 2006.
	WU Jinshui, LIN Qimei, HUANG Qiaoyun.. Determination method of soil microbial biomass and its application[M]. Beijing: China Meteorological Press, 2006.
[69]	康贻军, 胡健, 董必慧, 等. 滩涂盐碱土壤微生物生态特征的研究[J]. 农业环境科学学报, 2007, 26(S1): 181-183.
	KANG Yijun, HU Jian, DONG Bihui, et al. Microbial characters of a Salina-alkali soil in coastal wetland[J]. Journal of Agro-Environment Science, 2007, 26(S1): 181-183.
[70]	Tripathi S, Chakraborty A, Chakrabarti K, et al. Enzyme activities and microbial biomass in coastal soils of India[J]. Soil Biology and Biochemistry, 2007, 39(11): 2840-2848.
[71]	王飞. 盐分对土壤微生物多样性及土壤有机物转化的影响[D]. 石河子: 石河子大学, 2011.
	WANG Fei. Study on Soil Microbial Molecular Diversity and Organic Matter Transformation under Soil Salinization Condition[D]. Shihezi: Shihezi University, 2011.

序号 No.	文献出处 Sources of literature	发表时间 Publish time	对照类型 Control Type	影响因素 Influence factors	菌类 Mushrooms
1	马晓飞等^[15](新疆师范大学)	2015	农田土	①②③④	细菌、真菌
2	秦璐等^[16](新疆大学)	2013	农田土	①②③④⑤⑥	细菌、真菌
3	杨晓等^[17](青岛大学)	2016	农田土	①②	细菌、真菌
4	王鹭等^[18](兰州大学)	2011	高原土	①②⑥	细菌
5	伍文宪等^[19](四川省农业科学院)	2019	草地	④	细菌、真菌
6	王楠等^[20](东北林业大学)	2019	林地	②⑤	细菌、真菌
7	余炎炎等^[21](河南大学)	2020	高原土	①②③⑤⑥	细菌
8	孙弘哲等^[22](哈尔滨师范大学)	2018	林地	②⑤	细菌、真菌
9	车子涵等^[23](青海示范大学)	2020	高原	①②③⑤	细菌、真菌
10	段亚楠等^[24](山东农业大学)	2021	农田土	①⑥	细菌、真菌
11	陈末等^[25](新疆农业大学)	2020	湿地	②③⑥	细菌
12	林尤伟等^[26](东北林业大学)	2016	林地	②③⑤	细菌、真菌
13	张宝贵等^[27](中国科学院寒区旱区环境与工程研究所)	2012	高原	②③	细菌
14	李君锋等^[28](西北师范大学)	2012	草地	①③⑥	细菌
15	关键飞等^[29](牡丹江师范学院	2020	农田土	①④⑤	细菌
16	马大龙等^[30](哈尔滨师范大学)	2020	林地	①②⑥	细菌、真菌
17	陈泓硕等^[31](哈尔滨师范大学)	2020	农田土	①②④	细菌
18	李昌明等^[32](兰州大学)	2012	高原土	①②⑥	细菌
19	张宝贵等^[33](中国科学院寒区旱区环境与工程研究所)	2016	高原土	①②	细菌
20	董康等^[34](兰州交通大学)	2013	高原土	①②⑤	细菌、真菌
21	张宝贵等^[35](中国科学院寒区旱区环境与工程研究所)	2017	农田土	①②⑥	细菌
22	Dandan Song et al^[36](哈尔滨师范大学)	2022	林地	①②⑤	细菌、真菌
23	胡霞等^[37](乐山师范学院	2021	农田土	③⑤	细菌、真菌
24	孙嘉鸿等^[38](东北师范大学)	2022	农田土	①②③⑤	细菌、真菌
25	张超凡等^[39](东北师范大学)	2018	林地	③	细菌
26	薛烨飞等^[40](东北师范大学)	2021	农田土	④⑤	细菌、真菌
27	王奥等^[41](四川农业大学)	2012	林地	①③⑤	细菌、真菌
28	李淼等^[42](中国地质大学)	2011	高原土	①②⑤	细菌
29	姜霁珊等^[43](延边大学)	2019	林地	①②⑤	细菌、真菌
30	刘超等^[44](哈尔滨师范大学)	2021	林地	②③	细菌
31	王艳发等^[45](中国地质大学)	2016	高原土	①②	细菌、真菌
32	孙秦川等^[46](兰州交通大学)	2017	高原土	①②③④⑤	细菌
33	吴明辉等^[47](中国科学院西北生态环境资源研究院)	2021	高原土	①②③⑤	细菌
34	胡维刚等^[48](兰州大学)	2016	高原土	①②⑤	细菌、真菌
35	谭波等^[49](四川农业大学)	2014	林地	③	细菌、真菌
36	谭波等^[50](四川农业大学)	2013	林地	①③⑤	细菌、真菌
37	Patrick O.Sorensen et al^[51](波士顿大学)	2018	林地	①⑤	细菌、真菌
38	Grant Jensen et al^[52](滑铁卢大学)	2022	农田土	③⑤	细菌、真菌
39	Meisner Annelein et al^[53](隆德大学)	2021	农田土	①②③	细菌、真菌
40	Tsunehiro Watanabe et al^[54](北海道大学)	2019	林地	⑤⑥	细菌、真菌
41	xia Hu et al^[55](成都大学)	2017	高原土	③⑥	细菌、真菌
42	H.Wu et al^[56](卡尔斯鲁厄理工学院)	2012	高原土	②⑤⑥	细菌、真菌

序号 No.	文献出处 Sources of literature	发表时间 Publish time	对照类型 Control Type	影响因素 Influence factors	菌类 Mushrooms
1	马晓飞等^[15](新疆师范大学)	2015	农田土	①②③④	细菌、真菌
2	秦璐等^[16](新疆大学)	2013	农田土	①②③④⑤⑥	细菌、真菌
3	杨晓等^[17](青岛大学)	2016	农田土	①②	细菌、真菌
4	王鹭等^[18](兰州大学)	2011	高原土	①②⑥	细菌
5	伍文宪等^[19](四川省农业科学院)	2019	草地	④	细菌、真菌
6	王楠等^[20](东北林业大学)	2019	林地	②⑤	细菌、真菌
7	余炎炎等^[21](河南大学)	2020	高原土	①②③⑤⑥	细菌
8	孙弘哲等^[22](哈尔滨师范大学)	2018	林地	②⑤	细菌、真菌
9	车子涵等^[23](青海示范大学)	2020	高原	①②③⑤	细菌、真菌
10	段亚楠等^[24](山东农业大学)	2021	农田土	①⑥	细菌、真菌
11	陈末等^[25](新疆农业大学)	2020	湿地	②③⑥	细菌
12	林尤伟等^[26](东北林业大学)	2016	林地	②③⑤	细菌、真菌
13	张宝贵等^[27](中国科学院寒区旱区环境与工程研究所)	2012	高原	②③	细菌
14	李君锋等^[28](西北师范大学)	2012	草地	①③⑥	细菌
15	关键飞等^[29](牡丹江师范学院	2020	农田土	①④⑤	细菌
16	马大龙等^[30](哈尔滨师范大学)	2020	林地	①②⑥	细菌、真菌
17	陈泓硕等^[31](哈尔滨师范大学)	2020	农田土	①②④	细菌
18	李昌明等^[32](兰州大学)	2012	高原土	①②⑥	细菌
19	张宝贵等^[33](中国科学院寒区旱区环境与工程研究所)	2016	高原土	①②	细菌
20	董康等^[34](兰州交通大学)	2013	高原土	①②⑤	细菌、真菌
21	张宝贵等^[35](中国科学院寒区旱区环境与工程研究所)	2017	农田土	①②⑥	细菌
22	Dandan Song et al^[36](哈尔滨师范大学)	2022	林地	①②⑤	细菌、真菌
23	胡霞等^[37](乐山师范学院	2021	农田土	③⑤	细菌、真菌
24	孙嘉鸿等^[38](东北师范大学)	2022	农田土	①②③⑤	细菌、真菌
25	张超凡等^[39](东北师范大学)	2018	林地	③	细菌
26	薛烨飞等^[40](东北师范大学)	2021	农田土	④⑤	细菌、真菌
27	王奥等^[41](四川农业大学)	2012	林地	①③⑤	细菌、真菌
28	李淼等^[42](中国地质大学)	2011	高原土	①②⑤	细菌
29	姜霁珊等^[43](延边大学)	2019	林地	①②⑤	细菌、真菌
30	刘超等^[44](哈尔滨师范大学)	2021	林地	②③	细菌
31	王艳发等^[45](中国地质大学)	2016	高原土	①②	细菌、真菌
32	孙秦川等^[46](兰州交通大学)	2017	高原土	①②③④⑤	细菌
33	吴明辉等^[47](中国科学院西北生态环境资源研究院)	2021	高原土	①②③⑤	细菌
34	胡维刚等^[48](兰州大学)	2016	高原土	①②⑤	细菌、真菌
35	谭波等^[49](四川农业大学)	2014	林地	③	细菌、真菌
36	谭波等^[50](四川农业大学)	2013	林地	①③⑤	细菌、真菌
37	Patrick O.Sorensen et al^[51](波士顿大学)	2018	林地	①⑤	细菌、真菌
38	Grant Jensen et al^[52](滑铁卢大学)	2022	农田土	③⑤	细菌、真菌
39	Meisner Annelein et al^[53](隆德大学)	2021	农田土	①②③	细菌、真菌
40	Tsunehiro Watanabe et al^[54](北海道大学)	2019	林地	⑤⑥	细菌、真菌
41	xia Hu et al^[55](成都大学)	2017	高原土	③⑥	细菌、真菌
42	H.Wu et al^[56](卡尔斯鲁厄理工学院)	2012	高原土	②⑤⑥	细菌、真菌

	细菌Bacteria				真菌Fungi
	异质性 Heterogeneousness		整体效应 Overall effect		异质性 Heterogeneousness		整体效应 Overall effect
	P	I²	P	Z	P	I²	P	Z
温度 Temperature	0.94	0%	0.003	2.98	0.87	0%	0.02	2.37
pH值	0.87	0%	<0.000 01	4.59	0.69	0%	0.000 2	3.74
含水量 Water content	0.96	0%	<0.000 01	5.31	0.97	0%	0.001	3.85
有机质 Organic matter	0.86	0%	0.34	0.96	0.86	0%	0.35	0.94
有机氮 Organic N	0.94	0%	0.000 3	3.65	0.88	0%	0.05	1.95
全氮 Total N	0.91	0%	0.005	2.79	0.99	0%	0.05	1.98

	细菌Bacteria				真菌Fungi
	异质性 Heterogeneousness		整体效应 Overall effect		异质性 Heterogeneousness		整体效应 Overall effect
	P	I²	P	Z	P	I²	P	Z
温度 Temperature	0.94	0%	0.003	2.98	0.87	0%	0.02	2.37
pH值	0.87	0%	<0.000 01	4.59	0.69	0%	0.000 2	3.74
含水量 Water content	0.96	0%	<0.000 01	5.31	0.97	0%	0.001	3.85
有机质 Organic matter	0.86	0%	0.34	0.96	0.86	0%	0.35	0.94
有机氮 Organic N	0.94	0%	0.000 3	3.65	0.88	0%	0.05	1.95
全氮 Total N	0.91	0%	0.005	2.79	0.99	0%	0.05	1.98