Effects of Reclamation on the Composition and Structure of Soil Bacterial  Communities in Abandoned Salinized Farmland

doi:10.6048/j.issn.1001-4330.2018.04.017

Abstract

Abstract: 【Objective】 Abandoned salinized land in Xinjiang of China is being reclaimed for cotton production. The effect of reclamation on soil quality and function was obtained by comparing the changes of soil bacterial communities before and after reclamation.【Method】The specific objectives of this field study were: (1) to compare bacterial composition and diversity in unfarmed (i.e., unreclaimed) and cropped soils and (2) to explore the specific functions of the flora identified by Illumina MiSeq sequencing.【Result】Reclamation significantly reduced soil EC and available K, and significantly increased SOM, available N, and available P. Bacterial species richness and diversity increased when abandoned farmland was reclaimed for cotton production. Proteobacteria and Firmicutes were the dominant phyla in unfarmed soil. Reclamation reduced the relative abundance of Firmicutes, but increased the contents of Chloroflexi, Acidobacteria, and Actinobacteria. Cluster analyses showed that there was a significant difference in the bacterial community between the abandoned farmland and the cultivated land after the reclamation.【Conclusion】In general, cotton production after reclamation improved soil physical and chemical properties and changed the structure and composition of soil bacterial communities compared with unfarmed soil.

Key words: reclamation; soil bacterial community; MiSeq sequencing; bacterial diversity

摘要： 【目的】新疆盐碱弃耕地开垦后用于棉花的种植,研究比较开垦前后土壤细菌群落的变化,分析开垦对土壤质量和功能的影响。【方法】通过Illumina MiSeq测序,比较弃耕地与开垦后种植棉花的土壤细菌群落的组成和多样性;探索菌群所具有的特定功能。【结果】开垦显著地降低了土壤电导率和速效钾含量,显著地增加了土壤有机质、碱解氮和速效磷含量。弃耕地开垦为棉田后增加了细菌物种的丰富度和多样性。Proteobacteria和Firmicutes是弃耕地土壤中主要的细菌门。开垦降低了Firmicutes的相对丰度,但是增加了Chloroflexi, Acidobacteria和Actinobacteria的相对丰度。聚类分析显示弃耕地与开垦后农田的细菌群落有明显的差异性。【结论】开垦后进行棉花生产提高了土壤理化特性,改变了土壤细菌群落组成和结构。

关键词: 开垦, 土壤细菌群落, MiSeq测序, 细菌多样性

CLC Number:

S154.36

YANG Lei, ZHANG Yu-long, JIANG Tong-xuan, ZHANG Feng-hua. Effects of Reclamation on the Composition and Structure of Soil Bacterial Communities in Abandoned Salinized Farmland[J]. Xinjiang Agricultural Sciences, 2018, 55(4): 726-736.

杨磊,张玉龙,姜同轩,张凤华. 开垦对盐渍化弃耕地土壤细菌群落组成和结构的影响[J]. 新疆农业科学, 2018, 55(4): 726-736.

References

[1] Zhao, J., Zhang, R., Xue, C., Xun, W., Sun, L., & Xu, Y., et al. (2014). Pyrosequencing reveals contrasting soil bacterial diversity and community structure of two main winter wheat cropping systems in china. Microbial Ecology, 67(2): 443-453.
[2] Zhong, W., Gu, T., Wang, W., Zhang, B., Lin, X., & Huang, Q., et al. (2010). The effects of mineral fertilizer and organic manure on soil microbial community and diversity. Plant & Soil, 326(1-2): 511-523.
[3] Paul, E. A. (2014). Soil microbiology and biochemistry. Journal of Range Management, 51(2): 254.
[4] Quadros, P. D. D., Zhalnina, K., Davisrichardson, A., Fagen, J. R., Drew, J., & Bayer, C., et al. (2012). The effect of tillage system and crop rotation on soil microbial diversity and composition in a subtropical acrisol. Diversity, 4(4): 375-395.
[5] Qiu, M., Zhang, R., Xue, C., Zhang, S., Li, S., & Zhang, N., et al. (2012). Application of bio-organic fertilizer can control fusarium, wilt of cucumber plants by regulating microbial community of rhizosphere soil. Biology & Fertility of Soils, 48(7): 807-816.
[6] Johnsen, K., Jacobsen, C. S., Torsvik, V., & Sorenson, A. J. (2001). Pesticide effects of bacterial diversity in agricultural soils--a review. Biology & Fertility of Soils, 33(6): 443-453.
[7] Rietz, D. N., & Haynes, R. J. (2003). Effects of irrigation-induced salinity and sodicity on soil microbial activity. Soil Biology & Biochemistry, 35(6): 845-854.
[8] Liu, E., Yan, C., Mei, X., He, W., Bing, S. H., & Ding, L., et al. (2010). Long-term effect of chemical fertilizer, straw, and manure on soil chemical and biological properties in northwest china. Geoderma, 158(3-4): 173-180.
[9] Cui, J., Liu, C., Li, Z., Wang, L., Chen, X., & Ye, Z., et al. (2012). Long-term changes in topsoil chemical properties under centuries of cultivation after reclamation of coastal wetlands in the yangtze estuary, china. Soil & Tillage Research, 123(5): 50-60.
[10] Hartmann, M., Lee, S., Hallam, S., & Mohn, W. (2009). Bacterial, archaeal and eukaryal community structures throughout soil horizons of harvested and naturally disturbed forest stands. Environmental Microbiology, 11(12): 3,045-3,062.
[11] Will, C., Thürmer, A., Wollherr, A., Nacke, H., Herold, N., & Schrumpf, M., et al. (2010). Horizon-specific bacterial community composition of german grassland soils, as revealed by pyrosequencing-based analysis of 16s rrna genes. Applied & Environmental Microbiology, 76(20): 6,751-6,759.
[12] Oberauner, L., Zachow, C., Lackner, S., H?genauer, C., Smolle, K. H., & Berg, G. (2013). The ignored diversity: complex bacterial communities in intensive care units revealed by 16s pyrosequencing. Sci Rep, 3(3):1,413.
[13] Sun, Y., Cai, Y., Liu, L., Yu, F., & Farmerie, W. (2009). Esprit: estimating species richness using large collections of 16s rrna data. Nucleic Acids Research, 37(10):e76.
[14] ClaudioCiavatta, MarcoGovi, LiviaVittoriAntisari, & PaoloSequi. (1991). Determination of organic carbon in aqueous extracts of soils and fertilizers. Communications in Soil Science & Plant Analysis, 22(9-10):795-807.
[15] Mulvaney, R. L., & Khan, S. A. (2001). Diffusion methods to determine different forms of nitrogen in soil hydrolysates. Soil Science Society of America Journal, 65(4): 1,284-1,292.
[16] Page, A. L., Miller, R. H., & Keeney, D. R. (1982). Chemical and microbiological properties. American Society of Agronomy : Soil Science Society of America.
[17] Shen, Z., Zhong, S., Wang, Y., Wang, B., Mei, X., & Li, R., et al. (2013). Induced soil microbial suppression of banana fusarium wilt disease using compost and biofertilizers to improve yield and quality. European Journal of Soil Biology, 57(4):1-8.
[18] Yang, W., Zhao, H., Chen, X., Yin, S., Cheng, X., & An, S. (2013). Consequences of short-term c 4, plant spartina alterniflora, invasions for soil organic carbon dynamics in a coastal wetland of eastern china. Ecological Engineering, 61(12): 50-57.
[19] Vance, E. D., Brooks, P. C., & Jenkinson, D. S. (1987). An extraction method for measuring soil microbial biomass. Soil Biology & Biochemistry, 19(19): 703-707.
[20] Zhou, J., Bruns, M. A., & Tiedje, J. M. (1996). Dna recovery from soils of diverse composition. Appl Environ Microbiol, 62(2): 316-322.
[21] Xiong, J., Liu, Y., Lin, X., Zhang, H., Zeng, J., & Hou, J., et al. (2012). Geographic distance and ph drive bacterial distribution in alkaline lake sediments across tibetan plateau. Environmental Microbiology, 14(9):2,457-2,466.
[22] Edgar, R. C., Haas, B. J., Clemente, J. C., Quince, C., & Knight, R. (2011). Uchime improves sensitivity and speed of chimera detection. Bioinformatics, 27(16): 2,194-2,200.
[23] Quast, C., Pruesse, E., Yilmaz, P., Gerken, J., Schweer, T., & Yarza, P., et al. (2013). The silva ribosomal rna gene database project: improved data processing and web-based tools. Nucleic Acids Research,41(Database issue): 590-596.
[24] Schloss, P. D., Westcott, S. L., Ryabin, T., Hall, J. R., Hartmann, M., & Hollister, E. B., et al. (2009). Introducing mothur: open-source, platform-independent, community-supported software for describing and comparing microbial communities. Applied & Environmental Microbiology, 75(23): 7,537-7,541.
[25] Fouts, D. E., Szpakowski, S., Purushe, J., Torralba, M., Waterman, R. C., & Macneil, M. D., et al. (2012). Next generation sequencing to define prokaryotic and fungal diversity in the bovine rumen. Plos One, 7(11): e48289.
[26] Jiang, X. T., Peng, X., Deng, G. H., Sheng, H. F., Wang, Y., & Zhou, H. W., et al. (2013). Illumina sequencing of 16s RRNA tag revealed spatial variations of bacterial communities in a mangrove wetland. Microbial Ecology, 66(1): 96-104.
[27] 周丽, 王玉刚, 李彦,等. 盐碱荒地开垦年限对表层土壤盐分的影响[J]. 干旱区地理, 2013, 36(2): 285-291.
   ZHOU Li, WANG Yu-gang, LI Yan, et al. (2013). Effects of cultivation on soil salinity in upper soil profiles of the saline-alkali land[J]. Arid Land Geography, 36(2): 285-291. (in Chinese)
[28] Zhang, F. H., Yang, H. C., Gale, W. J., Cheng, Z. B., & Yan, J. H. (2016). Temporal changes in soil organic carbon and aggregate-associated organic carbon after reclamation of abandoned, salinized farmland. Journal of Agricultural Science, 155(2): 205-215.
[29] 王双磊. 棉花秸秆还田对盐碱地棉田土壤理化性质和生物学特性的影响[D]. 泰安:山东农业大学硕士学位论文, 2015.
   WANG Shuang-lei. (2015). Effect of cotton straw incorporated into saline-alkali soil on physical, chemical and biological properties [D]. Master Dissertation. Shandong Agricultural University, Taian. (in Chinese)
[30] 姜益娟, 郑德明, 吕双庆, 等. 连续施用棉籽饼和棉秆还田及化肥配施的培肥效应[J]. 干旱地区农业研究, 1999,(4): 19-24.
   JIANG Yi-juan, ZHENG De-ming, L Shuang-qin, ,et al. (1999). Fertilization effect of continuous application of cotton seed manure and cotton stalk returned to field together with combined application of chemical fertilizers [J]. Agricultural Research in The Arid Areas, (4): 19-24. (in Chinese)
[31] Zu, C., Li, Z. G., Yang, J. F., Yu, H., Sun, Y., & Tang, H. L., et al. (2014). Acid soil is associated with reduced yield, root growth and nutrient uptake in black pepper (piper nigrum l.). Journal of Agricultural Science, 5(5): 466-473.
[32] Lauber, C. L., Hamady, M., Knight, R., & Fierer, N. (2009). Pyrosequencing-based assessment of soil ph as a predictor of soil bacterial community structure at the continental scale. Applied & Environmental Microbiology, 75(15): 5,111-5,120.
[33] 王银亚, 李晨华, 马健. 开垦对荒漠土壤微生物群落结构特征的影响[J]. 中国沙漠, 2017, 37(3): 514-522.
   WANG Yin-ya, LI Chen-hua, MA Jian. (2017). Effects of Desert Rclamation on Soil Microbial ommunity and Microbial Diversity [J]. Journal of Desert Research, 37(3): 514-522. (in Chinese)
[34] Wilkinson, S. C., Anderson, J. M., Scardelis, S. P., Tisiafouli, M., Taylor, A., & Wolters, V. (2002). Plfa profiles of microbial communities in decomposing conifer litters subject to moisture stress. Soil Biology & Biochemistry, 34(2): 189-200.
[35] Garbeva, P., van Veen, J. A., & van Elsas, J. D. (2004). Microbail diversity in soil: selection of microbial populations by plant and soil type and implications for disease suppressiveness. Annual Review of Phytopathology, 42(42): 243-270.
[36] Wieland, G., Neumann, R., & Backhaus, H. (2001). Variation of microbial communities in soil, rhizosphere, and rhizoplane in response to crop species, soil type, and crop development. Applied & Environmental Microbiology, 67(12):5,849-5,854.
[37] Sudini, H., Liles, M. R., Arias, C. R., Bowen, K. L., & Huettel, R. N. (2011). Exploring soil bacterial communities in different peanut-cropping sequences using multiple molecular approaches. Phytopathology, 101(7): 819-827.
[38] Kberl, M., Müller, H., Ramadan, E. M., & Berg, G. (2011). Desert farming benefits from microbial potential in arid soils and promotes diversity and plant health. Plos One, 6(9): e24452.
[39] Mendes, R., Kruijt, M., De, B. I., Dekkers, E., Van, d. V. M., & Schneider, J. H., et al. (2011). Deciphering the rhizosphere microbiome for disease-suppressive bacteria. Science, 332(6033):1,097-1,100.

Effects of Reclamation on the Composition and Structure of Soil Bacterial Communities in Abandoned Salinized Farmland

开垦对盐渍化弃耕地土壤细菌群落组成和结构的影响

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